Standard name | |
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Human Ortholog | |
Description | S-adenosylmethionine-dependent methyltransferase; seven-beta-strand lysine methyltransferase which dimethylates translation elongation factor EF2 (Eft1p and Eft2p) at lysine 613 and methylates EF3 (Yef3p) at lysine 187; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; involved in regulation of translational termination; predicted involvement in ribosome biogenesis |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 2 | 2 |
Bud | 1 | 0 | 3 | 3 | 1 | 3 | 20 | 6 | 10 | 21 | 23 | 9 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 6 | 0 | 6 | 11 |
Bud Neck | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 2 | 0 | 4 | 1 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 14 | 5 | 8 | 5 | 5 | 8 | 5 | 5 | 0 | 5 | 5 | 8 | 1 | 8 | 12 | 12 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 300 | 163 | 437 | 383 | 557 | 518 | 896 | 843 | 424 | 569 | 314 | 345 | 411 | 737 | 647 | 196 | 217 | 234 | 298 | 160 | 402 | 317 | 485 | 520 |
Endoplasmic Reticulum | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 11 | 0 | 2 | 2 | 14 | 11 | 14 | 0 | 0 | 6 | 1 | 3 | 2 |
Endosome | 0 | 0 | 2 | 0 | 6 | 5 | 9 | 12 | 3 | 2 | 0 | 2 | 1 | 2 | 0 | 1 | 2 | 1 | 0 | 0 | 3 | 1 | 2 | 4 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 4 | 0 |
Mitochondria | 10 | 9 | 3 | 27 | 67 | 89 | 355 | 244 | 228 | 347 | 332 | 296 | 0 | 0 | 1 | 6 | 2 | 7 | 4 | 8 | 10 | 1 | 4 | 5 |
Nucleus | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 1 | 3 | 2 | 0 | 0 | 2 | 2 | 0 | 0 | 4 | 3 | 0 | 0 | 0 | 1 | 2 | 3 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 9 | 5 | 3 | 2 | 5 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 1 | 4 | 5 |
Vac/Vac Membrane | 1 | 0 | 3 | 2 | 5 | 1 | 10 | 23 | 4 | 13 | 5 | 10 | 0 | 0 | 2 | 2 | 3 | 5 | 6 | 1 | 0 | 0 | 0 | 1 |
Unique Cell Count | 307 | 170 | 444 | 388 | 582 | 556 | 1021 | 961 | 535 | 736 | 501 | 532 | 412 | 743 | 652 | 202 | 227 | 243 | 321 | 176 | 443 | 330 | 526 | 575 |
Labelled Cell Count | 316 | 175 | 465 | 421 | 645 | 626 | 1301 | 1151 | 682 | 969 | 681 | 685 | 420 | 755 | 655 | 227 | 252 | 277 | 321 | 176 | 443 | 330 | 526 | 575 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.1 | 4.5 | 4.6 | 4.3 | 4.2 | 3.6 | 3.5 | 3.6 | 3.5 | 3.0 | 2.9 | 3.0 | 5.7 | 5.9 | 5.7 | 7.2 | 7.4 | 6.5 | 6.0 | 6.3 | 6.3 |
Std Deviation (1e-4) | 0.5 | 0.9 | 1.1 | 1.4 | 1.4 | 0.8 | 1.4 | 1.4 | 1.5 | 1.1 | 0.8 | 0.6 | 0.8 | 0.9 | 1.1 | 1.2 | 1.2 | 1.1 | 1.1 | 1.4 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.11 | -0.35 | -0.4 | -0.35 | -0.39 | -0.61 | -0.68 | -0.63 | 0.31 | 0.35 | 0.3 | 0.65 | 0.69 | 0.49 | 0.39 | 0.46 | 0.46 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.3698 | 3.8732 | 3.613 | 3.0779 | 2.8607 | 3.4249 | -1.339 | -0.5548 | -1.0793 | -1.2858 | -0.08 | -1.033 | -1.2895 | -0.5638 | -0.9 | -0.6771 | -0.717 | -1.043 |
Actin | 0.0406 | 0.0004 | 0.0153 | 0.0015 | 0.0015 | 0.0055 | 0.0511 | 0.0002 | 0.0238 | 0.0001 | 0.0176 | 0.018 | 0.0382 | 0.0007 | 0.0181 | 0.0098 | 0.003 | 0.0014 |
Bud | 0.0013 | 0.0026 | 0.0067 | 0.0032 | 0.0022 | 0.0019 | 0.0036 | 0.0001 | 0.0029 | 0.0004 | 0.0179 | 0.0012 | 0.0018 | 0.0058 | 0.0101 | 0.0012 | 0.0236 | 0.0006 |
Bud Neck | 0.0072 | 0.0003 | 0.003 | 0.0004 | 0.0003 | 0.0008 | 0.0043 | 0.0001 | 0.0005 | 0.0001 | 0.017 | 0.001 | 0.0013 | 0.0001 | 0.0011 | 0.0008 | 0.0002 | 0.0002 |
Bud Periphery | 0.0039 | 0.0032 | 0.0039 | 0.0139 | 0.0103 | 0.0013 | 0.0075 | 0.0001 | 0.0059 | 0.0003 | 0.0563 | 0.003 | 0.0013 | 0.0013 | 0.0034 | 0.0017 | 0.0091 | 0.0037 |
Bud Site | 0.0101 | 0.0224 | 0.0097 | 0.0012 | 0.0002 | 0.0002 | 0.0151 | 0.0006 | 0.0068 | 0.0001 | 0.0426 | 0.0001 | 0.0044 | 0.0072 | 0.0074 | 0.0021 | 0.0004 | 0.0001 |
Cell Periphery | 0.0004 | 0.0004 | 0.0004 | 0.0002 | 0.0003 | 0.0002 | 0.0011 | 0.0002 | 0.0004 | 0 | 0.0027 | 0.0001 | 0.0005 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.0893 | 0.2845 | 0.2048 | 0.1755 | 0.1355 | 0.219 | 0.2402 | 0.4393 | 0.4246 | 0.4578 | 0.3028 | 0.504 | 0.1337 | 0.3403 | 0.2743 | 0.2504 | 0.3884 | 0.273 |
Cytoplasmic Foci | 0.0115 | 0.0055 | 0.0091 | 0.0094 | 0.017 | 0.0072 | 0.0382 | 0.008 | 0.0238 | 0.0116 | 0.0383 | 0.0119 | 0.019 | 0.0035 | 0.0095 | 0.007 | 0.0167 | 0.0189 |
Eisosomes | 0.0009 | 0.0001 | 0.0004 | 0.0001 | 0.0012 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0007 | 0.0001 | 0.0003 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0031 | 0.0013 | 0.0013 | 0.0018 | 0.0015 | 0.0017 | 0.006 | 0.0038 | 0.004 | 0.0018 | 0.0088 | 0.0026 | 0.0024 | 0.001 | 0.0015 | 0.0013 | 0.0031 | 0.0015 |
Endosome | 0.0202 | 0.0008 | 0.0027 | 0.0145 | 0.0011 | 0.0024 | 0.0191 | 0.0035 | 0.0176 | 0.0127 | 0.0671 | 0.0192 | 0.0132 | 0.001 | 0.002 | 0.0095 | 0.0352 | 0.0048 |
Golgi | 0.0056 | 0.0001 | 0.0013 | 0.0012 | 0.0003 | 0.0012 | 0.0078 | 0.0002 | 0.0023 | 0.0002 | 0.025 | 0.0013 | 0.0056 | 0.0001 | 0.0012 | 0.0104 | 0.0032 | 0.0003 |
Lipid Particles | 0.0069 | 0.0001 | 0.0011 | 0.0047 | 0.0014 | 0.0017 | 0.0103 | 0.0012 | 0.0031 | 0 | 0.0914 | 0.0004 | 0.0068 | 0.0001 | 0.0011 | 0.0011 | 0.0035 | 0.0006 |
Mitochondria | 0.0048 | 0.0003 | 0.0104 | 0.0032 | 0.001 | 0.0017 | 0.0021 | 0.0002 | 0.011 | 0.0002 | 0.009 | 0.0011 | 0.0045 | 0.0002 | 0.0014 | 0.0048 | 0.0033 | 0.0007 |
None | 0.7613 | 0.675 | 0.7173 | 0.7576 | 0.8198 | 0.7456 | 0.5584 | 0.5383 | 0.4563 | 0.5085 | 0.248 | 0.4308 | 0.7335 | 0.6361 | 0.6587 | 0.6957 | 0.4847 | 0.6887 |
Nuclear Periphery | 0.0059 | 0.0003 | 0.0009 | 0.0012 | 0.0008 | 0.0022 | 0.005 | 0.0007 | 0.0018 | 0.0007 | 0.0068 | 0.0008 | 0.0065 | 0.0003 | 0.0008 | 0.0004 | 0.0153 | 0.0021 |
Nucleolus | 0.0014 | 0.0002 | 0.0008 | 0.0017 | 0.0012 | 0.0003 | 0.0012 | 0.0001 | 0.001 | 0 | 0.0027 | 0.0001 | 0.0015 | 0.0001 | 0.0006 | 0.0001 | 0.0005 | 0.0001 |
Nucleus | 0.0051 | 0.0006 | 0.0047 | 0.002 | 0.0009 | 0.0014 | 0.0031 | 0.0007 | 0.0015 | 0.0009 | 0.0043 | 0.0007 | 0.007 | 0.0006 | 0.0023 | 0.0004 | 0.0021 | 0.0008 |
Peroxisomes | 0.0058 | 0.0002 | 0.0027 | 0.0028 | 0.0008 | 0.0035 | 0.0052 | 0.0001 | 0.0047 | 0 | 0.0114 | 0.0002 | 0.0091 | 0 | 0.0019 | 0.0015 | 0.0007 | 0.0001 |
Punctate Nuclear | 0.0117 | 0.0008 | 0.0018 | 0.0026 | 0.0017 | 0.0012 | 0.0158 | 0.0004 | 0.002 | 0.0004 | 0.0127 | 0.0005 | 0.0061 | 0.0002 | 0.0026 | 0.0003 | 0.0044 | 0.0015 |
Vacuole | 0.002 | 0.0007 | 0.0015 | 0.001 | 0.0008 | 0.0007 | 0.0038 | 0.0019 | 0.0049 | 0.0034 | 0.0143 | 0.0022 | 0.0019 | 0.0008 | 0.0012 | 0.0011 | 0.0017 | 0.0006 |
Vacuole Periphery | 0.001 | 0.0001 | 0.0002 | 0.0004 | 0.0002 | 0.0003 | 0.0008 | 0.0002 | 0.0009 | 0.0006 | 0.0031 | 0.0006 | 0.001 | 0.0001 | 0.0002 | 0.0003 | 0.0009 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 24.958 | 18.7881 | 11.618 | 15.1844 | 21.005 | 9.6276 | 10.2899 | 12.0201 | 11.5728 | 19.6007 |
Translational Efficiency | 0.9698 | 1.0772 | 1.6276 | 1.0717 | 0.884 | 1.9141 | 1.2111 | 0.8541 | 0.7481 | 0.8208 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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