Standard name
Human Ortholog
Description UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p; UBX7 has a paralog, UBX6, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.38 0.34 0.27 0.26 0.33 0.22 0.19 0.2 0.17 0.19 0.17 0.15 0.37 0.39 0.47 0.4 0.37 0.37 0.09 0.18 0.07 0.17 0.13 0.11
Endoplasmic Reticulum 0 0.32 0.07 0.13 0 0.05 0 0 0 0 0 0 0.5 0.16 0.09 0 0 0 0.55 0.43 0.57 0.39 0.44 0.44
Endosome 0.09 0 0.21 0.09 0.14 0 0 0.07 0.06 0 0 0 0 0.05 0.06 0.06 0.05 0.1 0 0 0 0 0.05 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.33 0.08 0.13 0.29 0.31 0.59 0.6 0.44 0.4 0.55 0.58 0.61 0 0 0 0 0 0 0.1 0.09 0.09 0.12 0.08 0.08
Nucleus 0 0 0 0 0 0 0 0.06 0.05 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0.05 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0.09 0.09 0.15 0.08 0.1 0.11
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.42 0.33 0.52 0.36 0.35 0.27 0.34 0.43 0.48 0.34 0.37 0.36 0.2 0.48 0.49 0.52 0.5 0.54 0 0.09 0 0.1 0.1 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 0 1 0 0 1 1 1 0 0 0 0 0 2 5 6 2 3 8 22 5 6 8
Bud 2 4 3 2 17 12 26 25 29 27 36 28 0 1 1 1 2 1 4 6 2 5 7 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 3 2 2 3 7
Bud Site 0 0 0 0 0 3 2 2 2 15 4 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 3 2 2 2 4 6 5 6 5 2 8 3 5 11 5 0 0 0 0 1 2
Cytoplasm 50 95 139 81 137 187 187 157 168 203 161 153 95 203 244 121 105 101 18 68 44 69 66 61
Endoplasmic Reticulum 3 88 34 42 15 44 15 8 17 9 3 17 126 86 49 5 5 5 112 161 383 156 217 250
Endosome 12 5 108 30 59 16 12 54 57 37 21 13 8 26 29 19 15 28 6 10 15 15 24 41
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 3 2 0 6 2 3 5
Mitochondria 43 23 65 91 126 511 605 353 389 596 557 603 0 0 1 2 10 4 20 33 62 49 41 44
Nucleus 0 2 0 4 6 22 30 49 49 75 55 29 5 13 15 8 7 6 0 2 0 5 2 1
Nuclear Periphery 0 2 10 15 1 34 25 37 51 22 17 42 0 5 4 7 6 2 18 32 101 33 48 61
Nucleolus 0 0 2 0 0 0 4 14 3 11 4 3 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 4 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 3 0 0 0
SpindlePole 0 1 3 2 1 1 1 0 3 3 3 1 0 1 1 1 0 1 3 4 5 6 10 14
Vac/Vac Membrane 55 91 266 113 143 236 337 342 473 369 349 356 51 252 258 158 143 149 9 34 17 39 51 52
Unique Cell Count 130 279 514 316 413 863 1002 802 983 1074 954 994 254 523 522 306 284 276 204 378 677 398 495 570
Labelled Cell Count 165 313 635 384 507 1069 1247 1046 1248 1372 1216 1254 287 596 607 333 311 309 204 378 677 398 495 570


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 6.0 5.7 5.2 5.4 4.6 4.6 4.9 4.9 4.6 4.4 4.5 7.3 7.4 7.2 9.0 8.6 8.9 7.5 8.1 8.5
Std Deviation (1e-4) 0.8 0.9 0.9 1.4 1.4 1.0 1.0 1.2 1.1 1.0 1.0 1.0 0.9 1.0 1.0 1.6 1.4 1.6 1.2 1.3 1.4
Intensity Change (Log2) – – – -0.13 -0.09 -0.31 -0.33 -0.22 -0.22 -0.31 -0.37 -0.36 0.36 0.36 0.33 0.65 0.58 0.64 0.4 0.5 0.56


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 3.7 0 0 3.1 3.0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.4 2.0 -2.3 -3.8 -3.2 -4.5 -3.7 -4.6 -5.4 2.9 4.0 6.6 3.7 2.9 2.8
Endoplasmic Reticulum 3.2 -2.0 0 0 -5.7 -4.9 0 0 0 13.8 4.9 1.6 -3.2 -3.0 -3.0
Endosome -4.3 -2.6 0 0 -7.7 -8.9 0 0 0 -6.5 -7.7 -7.3 -5.7 -5.9 -3.9
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 5.8 6.7 0 0 11.9 10.8 0 0 0 -5.9 -8.4 -8.2 -6.1 -4.2 -5.3
Nucleus 0 0 3.6 4.0 5.7 5.1 6.1 5.5 3.9 0 3.6 3.9 0 0 0
Nuclear Periphery 2.3 0 0 0 2.5 3.0 0 0 0 0 -1.3 -1.6 0.3 0.2 0
Nucleolus 0 0 0 0 2.2 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -4.5 -5.2 -9.1 -6.8 -3.2 -1.3 -6.6 -5.6 -6.0 -8.4 -1.1 -0.7 0 -0.4 0.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.2413 5.4979 5.4538 4.7235 4.8057 5.6133 3.9747 3.2534 3.4363 3.006 3.1618 3.6437 3.7571 2.8612 3.0809 2.7065 2.32 2.9511
Actin 0.0022 0.0008 0.0018 0.0015 0.0027 0.0015 0.0033 0.001 0.0027 0.0016 0.0107 0.0026 0.0346 0.0008 0.0017 0.0017 0.0435 0.0087
Bud 0.0001 0.0004 0.0018 0.0001 0.0011 0.0002 0.0002 0.002 0.0018 0.0001 0.0002 0.0001 0.0004 0.0032 0.0014 0.0078 0.0148 0.0017
Bud Neck 0.0003 0.0004 0.002 0.0027 0.0012 0.002 0.0032 0.0012 0.0029 0.002 0.0015 0.0028 0.0014 0.0005 0.001 0.0012 0.0078 0.004
Bud Periphery 0.0001 0.0002 0.0004 0.0001 0.0011 0.0002 0.0003 0.0004 0.0006 0.0001 0.0002 0.0002 0.0005 0.0005 0.0005 0.0023 0.0039 0.0009
Bud Site 0.0011 0.003 0.0015 0.0004 0.0012 0.0003 0.0023 0.0076 0.0026 0.0002 0.0007 0.0002 0.0028 0.0086 0.0083 0.0004 0.0013 0.0004
Cell Periphery 0.0005 0.0003 0.0003 0.0002 0.0003 0.0002 0.0001 0.0003 0.0002 0.0001 0.0001 0.0001 0.0002 0.0002 0.0002 0.0002 0.0002 0.0002
Cytoplasm 0.1937 0.334 0.2579 0.1932 0.2967 0.2968 0.1681 0.2996 0.2176 0.199 0.1707 0.2135 0.1917 0.3624 0.2776 0.3829 0.3549 0.3532
Cytoplasmic Foci 0.007 0.008 0.0151 0.0106 0.0127 0.0125 0.0098 0.0049 0.0063 0.0054 0.0032 0.007 0.0207 0.0066 0.0115 0.0115 0.0163 0.0152
Eisosomes 0.0002 0.0001 0.0001 0.0002 0.0001 0.0001 0 0 0 0 0.0001 0 0.0004 0 0.0001 0 0.0002 0.0001
Endoplasmic Reticulum 0.3013 0.1968 0.2482 0.314 0.2212 0.2654 0.2433 0.2452 0.2326 0.3205 0.2715 0.2595 0.1969 0.2659 0.2675 0.2161 0.194 0.2201
Endosome 0.0266 0.0349 0.0309 0.0453 0.0548 0.0333 0.0386 0.0247 0.0208 0.0264 0.0375 0.0234 0.052 0.0237 0.0331 0.0235 0.0394 0.0334
Golgi 0.0008 0.0006 0.0008 0.0007 0.0018 0.0009 0.0032 0.0005 0.0008 0.0005 0.0023 0.0005 0.0029 0.0004 0.0007 0.0005 0.0063 0.0027
Lipid Particles 0.0044 0.0024 0.0043 0.003 0.0052 0.004 0.009 0.0012 0.0011 0.0009 0.0026 0.0014 0.006 0.002 0.003 0.0017 0.0084 0.0045
Mitochondria 0.0016 0.0016 0.0016 0.0022 0.027 0.0021 0.0022 0.0013 0.0012 0.0012 0.0023 0.0012 0.002 0.0007 0.0021 0.0011 0.0024 0.0017
None 0.1218 0.1505 0.1485 0.1348 0.1014 0.125 0.03 0.0351 0.0484 0.0139 0.0071 0.0345 0.0497 0.0557 0.0935 0.1262 0.0346 0.0416
Nuclear Periphery 0.2455 0.1651 0.1945 0.2001 0.1733 0.1495 0.3444 0.2122 0.2814 0.3084 0.2923 0.2894 0.3188 0.1847 0.1838 0.1011 0.1457 0.1677
Nucleolus 0.0006 0.0009 0.001 0.0009 0.0008 0.001 0.0009 0.0022 0.0017 0.0004 0.0016 0.0008 0.0008 0.0008 0.0098 0.0011 0.0011 0.0009
Nucleus 0.0736 0.0714 0.0595 0.064 0.0756 0.0738 0.1118 0.124 0.1313 0.0886 0.1663 0.1307 0.0897 0.0623 0.0746 0.0764 0.0974 0.1035
Peroxisomes 0.0006 0.0002 0.0006 0.0003 0.0015 0.0006 0.0032 0.0001 0.0004 0.0002 0.0005 0.0002 0.0026 0.0001 0.0003 0.0003 0.0087 0.0015
Punctate Nuclear 0.0061 0.0121 0.0168 0.0136 0.0101 0.0205 0.0152 0.0246 0.0365 0.0212 0.0153 0.024 0.0136 0.0074 0.0191 0.0372 0.0124 0.0288
Vacuole 0.008 0.0122 0.0089 0.0084 0.007 0.0074 0.007 0.0089 0.0055 0.0054 0.0098 0.0053 0.0078 0.0108 0.0071 0.0051 0.0048 0.0068
Vacuole Periphery 0.0038 0.004 0.0038 0.0036 0.0031 0.0028 0.004 0.0027 0.0034 0.0039 0.0034 0.0029 0.0044 0.0027 0.0031 0.0017 0.0019 0.0024

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.6248 15.0477 17.3074 22.1174 11.458 15.9825 15.5951 24.1319 30.6876 22.06
Translational Efficiency 2.5146 1.7472 1.5834 1.7804 2.8911 2.1775 1.7528 1.5522 1.437 1.653

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p; UBX7 has a paralog, UBX6, that arose from the whole genome duplication
Localization
Cell Percentages ER (74%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Ubx7

Ubx7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ubx7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available