Standard name | |
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Human Ortholog | |
Description | Serine/threonine kinase and DNA damage checkpoint effector; mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 3 | 0 | 2 | 1 | 2 | 1 |
Bud | 0 | 0 | 0 | 1 | 2 | 2 | 5 | 1 | 6 | 8 | 2 | 4 | 0 | 0 | 0 | – | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 4 |
Bud Neck | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 6 | 5 | 3 | 2 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 0 | 1 | 0 | – | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 120 | 155 | 86 | 101 | 116 | 157 | 195 | 278 | 137 | 182 | 82 | 106 | 66 | 176 | 153 | – | 176 | 99 | 135 | 142 | 63 | 84 | 150 | 119 |
Endoplasmic Reticulum | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | – | 13 | 6 | 7 | 0 | 0 | 0 | 0 | 0 |
Endosome | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | – | 9 | 5 | 5 | 2 | 2 | 0 | 0 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 0 |
Mitochondria | 14 | 24 | 17 | 19 | 19 | 60 | 64 | 109 | 96 | 107 | 62 | 83 | 4 | 1 | 1 | – | 14 | 7 | 19 | 13 | 14 | 2 | 4 | 6 |
Nucleus | 0 | 2 | 0 | 1 | 1 | 0 | 1 | 2 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | – | 15 | 24 | 1 | 6 | 2 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 0 | 1 | 3 | 5 | 5 | 2 | 9 | 2 | 7 | 1 | 0 | 0 | – | 2 | 1 | 3 | 4 | 2 | 0 | 0 | 1 |
Vac/Vac Membrane | 2 | 3 | 5 | 1 | 0 | 1 | 0 | 3 | 1 | 3 | 0 | 0 | 2 | 4 | 6 | – | 45 | 40 | 5 | 5 | 4 | 0 | 2 | 5 |
Unique Cell Count | 131 | 174 | 99 | 110 | 124 | 192 | 230 | 336 | 200 | 244 | 126 | 154 | 71 | 182 | 162 | 241 | 164 | 192 | 186 | 99 | 95 | 167 | 147 | |
Labelled Cell Count | 139 | 188 | 111 | 124 | 140 | 225 | 271 | 407 | 250 | 315 | 150 | 205 | 75 | 183 | 165 | 278 | 184 | 192 | 186 | 99 | 95 | 167 | 147 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.2 | 3.2 | 3.7 | 3.0 | 3.2 | 2.9 | 2.5 | 3.0 | 2.7 | 2.5 | 2.7 | 2.4 | 4.3 | 4.2 | 4.3 | 4.9 | 5.5 | 5.8 | 3.9 | 4.1 | 5.0 |
Std Deviation (1e-4) | 0.9 | 0.7 | 1.9 | 1.0 | 1.4 | 1.3 | 1.4 | 1.6 | 1.5 | 1.6 | 1.7 | 1.1 | 1.9 | 1.6 | 1.9 | 1.5 | 1.7 | 1.1 | 1.2 | 1.6 | 2.2 |
Intensity Change (Log2) | – | – | – | -0.3 | -0.21 | -0.36 | -0.57 | -0.29 | -0.48 | -0.56 | -0.46 | -0.66 | 0.21 | 0.17 | 0.21 | 0.38 | 0.57 | 0.64 | 0.06 | 0.12 | 0.42 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | 3.2 | 4.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.0218 | 0.9252 | 0.6434 | 0.3191 | 0.2486 | 0.4383 | 0.0788 | -0.2734 | 0.2784 | 0.1009 | -0.17 | 0.1905 | -0.1579 | 0.6076 | 0.2294 | 0.4846 | -0.1494 | 0.0178 |
Actin | 0 | 0.0259 | 0.0021 | 0.0003 | 0.0001 | 0.0012 | 0.0401 | 0.0003 | 0.0256 | 0.0124 | 0.0003 | 0.0032 | 0.0008 | 0.0004 | 0.001 | 0.0001 | 0.0004 | 0.0001 |
Bud | 0 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0011 | 0.0004 | 0.0002 | 0.0016 | 0.0008 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Bud Neck | 0 | 0.0009 | 0.0002 | 0.0001 | 0.0001 | 0.002 | 0.0147 | 0 | 0.0005 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 |
Bud Periphery | 0 | 0.0003 | 0.0002 | 0.0003 | 0 | 0.0007 | 0.0018 | 0.0005 | 0.0003 | 0.0028 | 0.0009 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Bud Site | 0 | 0.018 | 0.0007 | 0.0001 | 0 | 0.0006 | 0.036 | 0.0004 | 0.0027 | 0.0005 | 0.0016 | 0.0001 | 0.0002 | 0.0002 | 0.0004 | 0 | 0.0001 | 0 |
Cell Periphery | 0 | 0.0005 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0011 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 |
Cytoplasm | 0.0326 | 0.0431 | 0.0671 | 0.0282 | 0.0669 | 0.0487 | 0.0263 | 0.0399 | 0.0178 | 0.0076 | 0.0586 | 0.0266 | 0.0575 | 0.0558 | 0.0533 | 0.047 | 0.171 | 0.0967 |
Cytoplasmic Foci | 0.0004 | 0.0022 | 0.0116 | 0.0286 | 0.0011 | 0.0173 | 0.0277 | 0.0046 | 0.0112 | 0.0053 | 0.005 | 0.0269 | 0.007 | 0.0034 | 0.0061 | 0.0016 | 0.0019 | 0.0022 |
Eisosomes | 0.0001 | 0.0011 | 0.0004 | 0.0008 | 0.0001 | 0.0002 | 0.0017 | 0.0003 | 0.0008 | 0.0007 | 0.0002 | 0.0002 | 0.0003 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0003 | 0.0009 | 0.001 | 0.0016 | 0.0006 | 0.0006 | 0.0035 | 0.0008 | 0.0006 | 0.0007 | 0.0011 | 0.0003 | 0.002 | 0.0042 | 0.0008 | 0.0004 | 0.0034 | 0.0005 |
Endosome | 0 | 0.0006 | 0.0056 | 0.0167 | 0.0004 | 0.0004 | 0.0271 | 0.0047 | 0.0112 | 0.0146 | 0.0142 | 0.0084 | 0.0132 | 0.026 | 0.0019 | 0.0002 | 0.0019 | 0.0007 |
Golgi | 0 | 0.0007 | 0.0013 | 0.0013 | 0 | 0.0001 | 0.0101 | 0.0006 | 0.0098 | 0.0403 | 0.0017 | 0.0082 | 0.0004 | 0.0026 | 0.0024 | 0 | 0.0001 | 0 |
Lipid Particles | 0 | 0.0004 | 0.0164 | 0.0042 | 0.0001 | 0.0005 | 0.0178 | 0.0004 | 0.0059 | 0.0024 | 0.0015 | 0.0162 | 0.0009 | 0.0005 | 0.0066 | 0.0001 | 0.0001 | 0 |
Mitochondria | 0 | 0.0015 | 0.0027 | 0.0024 | 0.0001 | 0.0002 | 0.0521 | 0.0074 | 0.0278 | 0.0576 | 0.0574 | 0.0182 | 0.0002 | 0.0015 | 0.0051 | 0.0001 | 0.0003 | 0.0001 |
None | 0.9663 | 0.9018 | 0.8557 | 0.8247 | 0.8559 | 0.9227 | 0.6926 | 0.9361 | 0.8601 | 0.8434 | 0.8121 | 0.8733 | 0.8942 | 0.901 | 0.8938 | 0.9489 | 0.7345 | 0.8959 |
Nuclear Periphery | 0 | 0.0003 | 0.0029 | 0.0016 | 0.0014 | 0.0001 | 0.004 | 0.0003 | 0.0003 | 0.001 | 0.0014 | 0.0001 | 0.0057 | 0.0018 | 0.0006 | 0.0002 | 0.0119 | 0.0002 |
Nucleolus | 0 | 0.0001 | 0.0006 | 0.0006 | 0.0005 | 0.0003 | 0.0037 | 0.0001 | 0.0003 | 0.0006 | 0.0006 | 0.0002 | 0.0004 | 0.0001 | 0.0008 | 0.0001 | 0.0005 | 0.0001 |
Nucleus | 0.0001 | 0.0004 | 0.0054 | 0.0827 | 0.0691 | 0.0004 | 0.0025 | 0.0004 | 0.0191 | 0.0016 | 0.0211 | 0.0001 | 0.0046 | 0.0003 | 0.0161 | 0.0004 | 0.0648 | 0.0014 |
Peroxisomes | 0 | 0.0004 | 0.0138 | 0.002 | 0 | 0.0004 | 0.0239 | 0.0006 | 0.0038 | 0.0023 | 0.0031 | 0.0158 | 0.0001 | 0.0001 | 0.0051 | 0 | 0 | 0.0001 |
Punctate Nuclear | 0 | 0.0006 | 0.011 | 0.0014 | 0.0032 | 0.0032 | 0.0087 | 0.0002 | 0.0004 | 0.0025 | 0.002 | 0.0009 | 0.0073 | 0.0002 | 0.0052 | 0.0004 | 0.008 | 0.0017 |
Vacuole | 0 | 0.0002 | 0.0008 | 0.0015 | 0.0003 | 0.0002 | 0.0027 | 0.0012 | 0.0009 | 0.0008 | 0.0141 | 0.0005 | 0.0048 | 0.0009 | 0.0004 | 0.0001 | 0.0004 | 0.0002 |
Vacuole Periphery | 0 | 0 | 0.0002 | 0.0005 | 0 | 0 | 0.001 | 0.0005 | 0.0006 | 0.0011 | 0.0019 | 0.0003 | 0.0003 | 0.0007 | 0.0001 | 0 | 0.0001 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 12.1904 | 15.5678 | 9.7037 | 15.896 | 13.999 | 9.7469 | 13.0647 | 12.1112 | 15.9712 | 17.0358 |
Translational Efficiency | 0.6642 | 0.5483 | 0.8527 | 0.5178 | 0.5348 | 0.8818 | 0.718 | 0.798 | 0.5622 | 0.4216 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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