Standard name | |
---|---|
Human Ortholog | |
Description | Protein that binds to the Rap1p C-terminus; acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; involved in control of DNA replication; contributes to resection of DNA double strand breaks (DSBs); deletion results in telomere elongation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 4 | 7 | 2 | 5 | 3 |
Bud | 0 | 0 | 5 | 12 | 12 | 13 | 26 | 27 | 19 | – | 29 | 23 | 0 | 1 | 7 | 1 | 1 | 2 | 2 | 2 | 6 | 0 | 8 | 2 |
Bud Neck | 1 | 0 | 8 | 0 | 4 | 4 | 4 | 4 | 1 | – | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 2 | 1 |
Bud Site | 1 | 0 | 1 | 2 | 4 | 4 | 11 | 17 | 7 | – | 6 | 16 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 23 | 29 | 138 | 88 | 76 | 92 | 99 | 124 | 68 | – | 44 | 50 | 78 | 103 | 132 | 43 | 46 | 54 | 5 | 12 | 47 | 24 | 46 | 29 |
Endoplasmic Reticulum | 3 | 3 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | – | 0 | 0 | 0 | 2 | 4 | 7 | 9 | 13 | 0 | 1 | 6 | 2 | 2 | 6 |
Endosome | 95 | 55 | 34 | 13 | 72 | 14 | 23 | 16 | 4 | – | 3 | 2 | 45 | 66 | 30 | 44 | 75 | 46 | 97 | 99 | 131 | 62 | 104 | 98 |
Golgi | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 5 | 2 | 1 | 2 | 0 | 1 |
Mitochondria | 15 | 67 | 111 | 180 | 138 | 284 | 344 | 471 | 340 | – | 284 | 401 | 39 | 16 | 81 | 17 | 8 | 18 | 25 | 32 | 94 | 33 | 52 | 46 |
Nucleus | 10 | 4 | 4 | 4 | 7 | 9 | 24 | 29 | 16 | – | 19 | 21 | 19 | 27 | 17 | 5 | 4 | 2 | 6 | 5 | 4 | 4 | 5 | 0 |
Nuclear Periphery | 15 | 7 | 0 | 2 | 0 | 0 | 2 | 5 | 2 | – | 1 | 2 | 4 | 2 | 2 | 0 | 1 | 3 | 26 | 24 | 19 | 7 | 9 | 6 |
Nucleolus | 8 | 3 | 1 | 2 | 3 | 2 | 5 | 10 | 9 | – | 5 | 8 | 7 | 0 | 0 | 2 | 1 | 4 | 7 | 9 | 9 | 5 | 6 | 4 |
Peroxisomes | 1 | 0 | 2 | 4 | 16 | 3 | 10 | 4 | 1 | – | 0 | 2 | 0 | 1 | 0 | 2 | 1 | 3 | 2 | 1 | 7 | 3 | 2 | 5 |
SpindlePole | 47 | 13 | 55 | 35 | 60 | 45 | 74 | 59 | 26 | – | 21 | 30 | 21 | 21 | 12 | 25 | 40 | 34 | 79 | 71 | 94 | 72 | 101 | 89 |
Vac/Vac Membrane | 44 | 83 | 7 | 9 | 40 | 15 | 16 | 23 | 8 | – | 13 | 16 | 50 | 29 | 33 | 22 | 27 | 19 | 11 | 16 | 24 | 11 | 24 | 14 |
Unique Cell Count | 199 | 213 | 323 | 300 | 329 | 410 | 489 | 637 | 409 | 334 | 456 | 224 | 242 | 285 | 123 | 165 | 151 | 287 | 302 | 492 | 252 | 396 | 327 | |
Labelled Cell Count | 264 | 266 | 367 | 351 | 432 | 486 | 639 | 790 | 501 | 425 | 572 | 263 | 270 | 318 | 169 | 215 | 202 | 287 | 302 | 492 | 252 | 396 | 327 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.9 | 4.1 | 3.7 | 3.4 | 3.8 | 3.4 | 3.1 | 3.2 | 2.8 | – | 3.0 | 2.7 | 4.3 | 4.3 | 3.7 | 5.7 | 6.3 | 5.8 | 4.3 | 4.3 | 4.7 |
Std Deviation (1e-4) | 0.7 | 0.9 | 1.6 | 1.3 | 1.4 | 1.6 | 0.9 | 1.3 | 1.2 | – | 1.4 | 0.9 | 1.1 | 1.1 | 1.1 | 1.7 | 2.5 | 2.0 | 1.3 | 1.2 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.11 | 0.03 | -0.14 | -0.26 | -0.2 | -0.4 | – | -0.32 | -0.47 | 0.21 | 0.21 | 0.02 | 0.62 | 0.77 | 0.65 | 0.23 | 0.21 | 0.33 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 6.0 | 5.8 | 4.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.2515 | 3.5162 | 3.4635 | 2.8496 | 3.8622 | 3.5664 | 2.5303 | 2.4385 | 2.3306 | 2.8387 | 1.7211 | 2.0744 | 3.5495 | 3.5869 | 3.192 | 3.4163 | 3.7652 | 3.3274 |
Actin | 0.0275 | 0.0028 | 0.0172 | 0.0195 | 0.0185 | 0.0042 | 0.0486 | 0.0028 | 0.0151 | 0.0359 | 0.0014 | 0.004 | 0.0183 | 0.0014 | 0.0096 | 0.0048 | 0.0025 | 0.0019 |
Bud | 0.0008 | 0.0037 | 0.0003 | 0.0002 | 0.0004 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0019 | 0 | 0.0048 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 |
Bud Neck | 0.0029 | 0.0019 | 0.0018 | 0.0021 | 0.0115 | 0.0105 | 0.0007 | 0.0012 | 0.0009 | 0.0135 | 0.001 | 0.0042 | 0.0014 | 0.0006 | 0.0008 | 0.0003 | 0.0023 | 0.006 |
Bud Periphery | 0.0018 | 0.0007 | 0.0007 | 0.0003 | 0.0008 | 0.0004 | 0.0005 | 0.0002 | 0.0002 | 0.0027 | 0.0001 | 0.0025 | 0.0005 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0003 |
Bud Site | 0.0033 | 0.0047 | 0.004 | 0.0007 | 0.0016 | 0.0006 | 0.001 | 0.0016 | 0.0012 | 0.0193 | 0.0002 | 0.0005 | 0.0038 | 0.0005 | 0.0009 | 0.0001 | 0.001 | 0.0003 |
Cell Periphery | 0.0008 | 0.0003 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.001 | 0 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 |
Cytoplasm | 0.0425 | 0.0417 | 0.0223 | 0.0023 | 0.049 | 0.0241 | 0.0503 | 0.0307 | 0.0113 | 0.0032 | 0.0286 | 0.0133 | 0.0124 | 0.0181 | 0.0134 | 0.0102 | 0.0051 | 0.0134 |
Cytoplasmic Foci | 0.0343 | 0.0158 | 0.0213 | 0.0206 | 0.0275 | 0.0104 | 0.0146 | 0.0113 | 0.0228 | 0.052 | 0.0226 | 0.0068 | 0.0179 | 0.0068 | 0.0141 | 0.0165 | 0.0442 | 0.0079 |
Eisosomes | 0.0008 | 0.0001 | 0.0003 | 0.0002 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0.0001 |
Endoplasmic Reticulum | 0.0179 | 0.0105 | 0.0057 | 0.0024 | 0.01 | 0.0075 | 0.0081 | 0.005 | 0.0026 | 0.0015 | 0.001 | 0.0015 | 0.0057 | 0.0058 | 0.0019 | 0.0022 | 0.0017 | 0.0034 |
Endosome | 0.0388 | 0.0281 | 0.0183 | 0.0124 | 0.0362 | 0.0156 | 0.0337 | 0.0124 | 0.0083 | 0.0505 | 0.0135 | 0.0084 | 0.0327 | 0.0071 | 0.0119 | 0.0066 | 0.0166 | 0.0153 |
Golgi | 0.0101 | 0.0027 | 0.0045 | 0.0056 | 0.0215 | 0.0052 | 0.0116 | 0.0015 | 0.003 | 0.0371 | 0.004 | 0.0058 | 0.0138 | 0.0019 | 0.0031 | 0.0027 | 0.0054 | 0.0026 |
Lipid Particles | 0.0476 | 0.0521 | 0.0377 | 0.0846 | 0.1062 | 0.0333 | 0.0185 | 0.0126 | 0.0388 | 0.0625 | 0.1312 | 0.0182 | 0.0467 | 0.0385 | 0.0396 | 0.04 | 0.1336 | 0.0206 |
Mitochondria | 0.0412 | 0.0015 | 0.0026 | 0.0009 | 0.0223 | 0.0076 | 0.0032 | 0.0008 | 0.0009 | 0.0628 | 0.0003 | 0.0041 | 0.0059 | 0.0007 | 0.0027 | 0.0006 | 0.0009 | 0.0008 |
None | 0.0635 | 0.0338 | 0.0215 | 0.0033 | 0.0694 | 0.023 | 0.0471 | 0.0218 | 0.029 | 0.0034 | 0.0041 | 0.0259 | 0.017 | 0.0396 | 0.0147 | 0.0466 | 0.0524 | 0.0287 |
Nuclear Periphery | 0.2667 | 0.2366 | 0.2783 | 0.2516 | 0.3057 | 0.1976 | 0.2087 | 0.2224 | 0.2264 | 0.1123 | 0.2001 | 0.2478 | 0.3824 | 0.3358 | 0.363 | 0.3142 | 0.1847 | 0.2722 |
Nucleolus | 0.0264 | 0.0306 | 0.0375 | 0.0136 | 0.0066 | 0.0601 | 0.0111 | 0.0244 | 0.0205 | 0.0075 | 0.0447 | 0.0378 | 0.0148 | 0.0162 | 0.0304 | 0.0078 | 0.0489 | 0.0619 |
Nucleus | 0.1119 | 0.2087 | 0.1019 | 0.0686 | 0.0743 | 0.2942 | 0.218 | 0.2682 | 0.2203 | 0.1266 | 0.1647 | 0.2508 | 0.1314 | 0.2832 | 0.1091 | 0.0348 | 0.0441 | 0.2452 |
Peroxisomes | 0.0163 | 0.0023 | 0.0048 | 0.0122 | 0.0181 | 0.0016 | 0.0031 | 0.0014 | 0.0043 | 0.0319 | 0.0059 | 0.003 | 0.0088 | 0.0015 | 0.0039 | 0.0065 | 0.0215 | 0.0016 |
Punctate Nuclear | 0.2278 | 0.3116 | 0.4123 | 0.4953 | 0.2086 | 0.2976 | 0.3163 | 0.3769 | 0.3898 | 0.3672 | 0.3734 | 0.3573 | 0.2798 | 0.2367 | 0.3757 | 0.5019 | 0.4308 | 0.3144 |
Vacuole | 0.009 | 0.0067 | 0.0027 | 0.0008 | 0.0042 | 0.0034 | 0.0015 | 0.0022 | 0.0016 | 0.004 | 0.0007 | 0.0013 | 0.0023 | 0.0015 | 0.0013 | 0.0008 | 0.0009 | 0.0014 |
Vacuole Periphery | 0.0083 | 0.003 | 0.004 | 0.0029 | 0.0069 | 0.0027 | 0.0027 | 0.0025 | 0.003 | 0.0022 | 0.0023 | 0.0018 | 0.0035 | 0.0037 | 0.0032 | 0.0031 | 0.0027 | 0.0018 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 28.1079 | 10.8054 | 8.2726 | 14.1345 | 19.5276 | 23.9696 | 13.4399 | 10.0074 | 12.311 | 26.8984 |
Translational Efficiency | 0.6609 | 0.6647 | 0.5357 | 0.3721 | 0.5536 | 0.7099 | 0.4789 | 0.4852 | 0.3728 | 0.4561 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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