Standard name
Human Ortholog
Description Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p)

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.96 0.94 1.0 0.94 0.92 0.93 0.92 0.83 0.73 0.77 0.99 0.99 0.99 0.9 0.89 0.88 0.9 0.95 0.82 0.82 0.74 0.67
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.08 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.05 0 0.09 0.14 0.08 0.33 0.47 0.31 0 0 0 0 0 0 0 0 0 0.06 0.09 0.09
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 0 0 0 1 1 0 0 0 0 0 1 0 0 1 2 5 10
Bud 0 1 1 1 0 1 1 2 5 7 7 1 1 1 0 0 0 0 0 2 2 10 22
Bud Neck 0 0 1 1 0 1 1 2 0 0 3 0 0 0 0 0 0 0 0 1 0 2 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 3 1 1 0 2 2 4 0 2 1 1 1 8 3 0 0 0 0 0 2 2
Cytoplasm 105 110 195 133 179 194 297 317 222 146 191 379 352 448 100 98 59 115 119 179 171 347 261
Endoplasmic Reticulum 0 2 3 0 1 1 1 1 2 1 2 0 0 1 7 9 2 0 0 2 5 6 5
Endosome 1 0 5 0 4 5 3 4 0 0 0 0 4 0 2 4 3 2 2 6 0 12 14
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 3 1 0 0 0 5 3
Mitochondria 2 2 1 6 4 19 46 26 89 94 76 3 0 2 4 2 2 2 1 9 13 41 35
Nucleus 1 0 2 1 2 2 3 3 1 0 8 1 0 1 1 2 1 0 0 2 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 1 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
SpindlePole 0 0 3 0 2 1 3 0 2 0 1 0 0 0 1 1 1 0 0 3 0 9 8
Vac/Vac Membrane 1 2 4 1 1 4 1 6 5 4 3 2 1 2 6 4 3 2 0 3 9 18 16
Unique Cell Count 109 114 207 133 191 210 321 343 269 200 249 381 356 453 111 110 67 129 126 218 210 470 390
Labelled Cell Count 110 117 218 145 194 228 359 363 331 253 295 387 359 456 134 125 76 129 126 218 210 470 390


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 5.0 5.7 5.1 5.5 4.5 4.6 5.0 4.2 3.8 4.1 5.4 5.4 5.4 9.1 9.9 8.5 5.2 4.9 5.3
Std Deviation (1e-4) 1.0 0.8 1.1 1.6 1.2 1.0 1.3 1.6 1.7 1.0 1.3 0.9 1.3 1.5 2.3 2.7 1.9 1.8 1.5 2.1
Intensity Change (Log2) -0.17 -0.06 -0.35 -0.33 -0.2 -0.44 -0.6 -0.47 -0.1 -0.09 -0.09 0.66 0.79 0.56 -0.15 -0.23 -0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.8 -0.2 -0.7 -0.7 -0.8 -3.8 -5.8 -5.2 4.0 3.2 3.5 -1.3 -1.6 -1.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 3.7 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.9153 2.9425 2.6653 2.1506 1.8444 2.7591 0.1786 1.3754 1.0833 0.9818 0.5236 0.9422 1.4052 2.2476 1.7737 1.8513 1.8882 1.6853
Actin 0.0275 0.0013 0.0112 0.0013 0.0003 0.0021 0.0085 0.0007 0.0091 0.04 0.0008 0.0009 0.0301 0.0071 0.0118 0.0114 0.0216 0.0108
Bud 0.001 0.0007 0.0014 0.0001 0.0001 0.0015 0.0014 0.0039 0.0007 0.026 0.0001 0.0028 0.0145 0.0264 0.0273 0.0115 0.024 0.0148
Bud Neck 0.0009 0.0004 0.0005 0.0011 0.0002 0.0039 0.0019 0.0003 0.0005 0.001 0.0002 0.0033 0.0015 0.0011 0.0013 0.0039 0.0012 0.0037
Bud Periphery 0.0049 0.0005 0.0015 0.0001 0.0001 0.0064 0.0014 0.0048 0.0006 0.0142 0.0001 0.0021 0.0097 0.0123 0.0156 0.0097 0.0192 0.0119
Bud Site 0.0017 0.0069 0.0016 0.0002 0.0001 0.0013 0.0091 0.0136 0.0045 0.0036 0 0.0004 0.0284 0.0914 0.0346 0.0091 0.003 0.0019
Cell Periphery 0.0003 0.0003 0.0002 0.0002 0.0004 0.0003 0.0003 0.0002 0.0001 0.0004 0 0.0001 0.0016 0.0016 0.0014 0.0008 0.0006 0.0005
Cytoplasm 0.286 0.4727 0.451 0.5474 0.4671 0.5841 0.4622 0.76 0.6819 0.5955 0.7108 0.7835 0.344 0.4931 0.4397 0.4254 0.5146 0.5548
Cytoplasmic Foci 0.0209 0.0119 0.0099 0.0117 0.0085 0.0067 0.0461 0.0113 0.0218 0.0347 0.0136 0.0152 0.0518 0.0182 0.0275 0.0206 0.0211 0.0297
Eisosomes 0.0008 0.0002 0.0002 0.0003 0.0003 0.0001 0.0002 0 0.0002 0.0001 0 0 0.0007 0.0003 0.0003 0.0002 0.0002 0.0002
Endoplasmic Reticulum 0.011 0.0045 0.0051 0.0046 0.0014 0.0034 0.0035 0.0022 0.0032 0.0023 0.0054 0.0021 0.0127 0.0025 0.0059 0.0066 0.0076 0.0055
Endosome 0.0095 0.0063 0.0051 0.0079 0.0015 0.0045 0.049 0.0038 0.016 0.0526 0.0101 0.0087 0.0245 0.0024 0.0082 0.0312 0.0114 0.0188
Golgi 0.003 0.0006 0.0007 0.0005 0.0001 0.0006 0.0168 0.0003 0.0106 0.0391 0.0004 0.0003 0.0117 0.0014 0.003 0.0122 0.0056 0.0054
Lipid Particles 0.0064 0.0005 0.0019 0.0023 0.0009 0.0003 0.0263 0.0001 0.0027 0.0047 0.0001 0.0001 0.0103 0.0008 0.005 0.0132 0.0094 0.0092
Mitochondria 0.0027 0.0006 0.0006 0.0008 0.0005 0.0013 0.0126 0.0003 0.0092 0.0052 0.0003 0.0003 0.0129 0.0016 0.0101 0.0096 0.0297 0.0052
None 0.5921 0.4773 0.4953 0.409 0.5149 0.377 0.3167 0.192 0.2284 0.1575 0.2524 0.1744 0.3987 0.3042 0.3536 0.3953 0.2789 0.2759
Nuclear Periphery 0.0035 0.0021 0.0016 0.0022 0.0004 0.0011 0.0034 0.0006 0.0011 0.0017 0.0012 0.0008 0.0069 0.0021 0.006 0.005 0.0153 0.0069
Nucleolus 0.0004 0.0004 0.0005 0.0002 0.0002 0.0002 0.0025 0.0001 0.0001 0.0002 0 0.0001 0.0033 0.0025 0.0024 0.0011 0.0012 0.0015
Nucleus 0.0022 0.0048 0.003 0.0068 0.0011 0.0025 0.0027 0.0026 0.0021 0.0067 0.0011 0.0021 0.0076 0.0096 0.0151 0.0138 0.016 0.0173
Peroxisomes 0.007 0.0005 0.0045 0.0003 0.0002 0.0003 0.0215 0.0004 0.0041 0.0066 0.0001 0.0002 0.0092 0.0014 0.0065 0.0015 0.002 0.0011
Punctate Nuclear 0.0156 0.0039 0.0015 0.0013 0.0006 0.0006 0.0075 0.0011 0.0009 0.0016 0.0004 0.0009 0.0134 0.017 0.0198 0.011 0.0113 0.021
Vacuole 0.0019 0.0031 0.0024 0.0015 0.001 0.0016 0.0044 0.0015 0.0016 0.0046 0.0023 0.0015 0.0052 0.0025 0.0041 0.0055 0.0038 0.0026
Vacuole Periphery 0.0006 0.0004 0.0003 0.0004 0.0001 0.0004 0.0016 0.0002 0.0004 0.0018 0.0005 0.0003 0.0013 0.0003 0.0009 0.0015 0.0022 0.0014

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 29.9179 17.9545 19.4294 26.6718 21.1581 28.8558 19.0993 22.9736 22.052 25.0711
Translational Efficiency 0.9694 1.0177 0.907 0.7102 0.7471 0.8527 0.8209 0.5992 0.6984 0.6232

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p)
Localization
Cell Percentages cytoplasm (80%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Dug2

Dug2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dug2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available