Standard name | |
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Human Ortholog | |
Description | Vacuolar aminopeptidase Y; processed to mature form by Prb1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 2 |
Bud | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 4 | 1 | 0 | 2 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 3 |
Bud Neck | 0 | 2 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 0 | 0 | 0 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 1 | 2 | 1 | 0 | 0 | 2 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 174 | 202 | 124 | 242 | 290 | 329 | 357 | 415 | 208 | 296 | 188 | 294 | 134 | 241 | 268 | 98 | 171 | 120 | 101 | 180 | 87 | 46 | 83 | 149 |
Endoplasmic Reticulum | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 5 | 18 | 9 | 4 | 0 | 2 | 0 | 1 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 0 | 1 | 0 | 2 | 0 | 2 | 2 | 2 | 0 | 2 | 6 | 2 | 1 | 0 | 0 | 2 |
Golgi | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 |
Mitochondria | 19 | 3 | 0 | 0 | 0 | 3 | 2 | 5 | 5 | 5 | 35 | 17 | 0 | 2 | 0 | 3 | 4 | 1 | 25 | 8 | 15 | 3 | 4 | 4 |
Nucleus | 0 | 0 | 1 | 1 | 0 | 8 | 11 | 10 | 10 | 2 | 5 | 4 | 2 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 5 | 3 | 1 | 2 | 46 | 48 | 93 | 46 | 30 | 54 | 24 | 36 | 0 | 2 | 0 | 14 | 21 | 15 | 41 | 21 | 25 | 15 | 24 | 41 |
Unique Cell Count | 186 | 208 | 126 | 249 | 313 | 362 | 403 | 448 | 232 | 331 | 222 | 330 | 137 | 250 | 273 | 110 | 193 | 135 | 191 | 221 | 141 | 71 | 125 | 217 |
Labelled Cell Count | 201 | 213 | 129 | 254 | 339 | 389 | 467 | 485 | 254 | 364 | 253 | 358 | 140 | 251 | 274 | 122 | 219 | 153 | 191 | 221 | 141 | 71 | 125 | 217 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.3 | 3.9 | 5.3 | 5.0 | 6.0 | 4.8 | 5.2 | 5.4 | 4.6 | 4.7 | 4.2 | 4.5 | 4.7 | 5.3 | 5.3 | 5.7 | 5.7 | 5.3 | 4.4 | 5.2 | 5.3 |
Std Deviation (1e-4) | 0.5 | 0.5 | 2.1 | 1.7 | 2.0 | 1.2 | 1.2 | 1.8 | 1.3 | 1.1 | 1.2 | 1.4 | 1.1 | 1.5 | 2.1 | 1.6 | 1.6 | 1.1 | 1.0 | 2.0 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.1 | 0.17 | -0.13 | -0.04 | 0.03 | -0.22 | -0.18 | -0.35 | -0.25 | -0.17 | -0.0 | -0.02 | 0.1 | 0.1 | 0.01 | -0.29 | -0.03 | 0.01 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 3.7 | 3.5 | 3.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.8527 | -0.5142 | -0.6435 | -0.1294 | -0.5451 | -0.4966 | -1.8007 | -0.9791 | -0.9215 | -1.3667 | -0.9183 | -1.2272 | -1.1359 | -1.0435 | -1.2442 | -0.3614 | -0.9353 | -1.2643 |
Actin | 0.0175 | 0.002 | 0.0051 | 0.0469 | 0.0132 | 0.0067 | 0.0164 | 0.0001 | 0.0008 | 0.0001 | 0.0059 | 0.0021 | 0.0208 | 0.0001 | 0.0015 | 0.0063 | 0.0091 | 0.0084 |
Bud | 0.0016 | 0.0062 | 0.002 | 0.0025 | 0.0019 | 0.0013 | 0.0018 | 0.0076 | 0.0064 | 0.0002 | 0.011 | 0.0001 | 0.0018 | 0.0009 | 0.0017 | 0.0014 | 0.0088 | 0.0003 |
Bud Neck | 0.0051 | 0.0005 | 0.0047 | 0.0016 | 0.007 | 0.0066 | 0.0004 | 0.0005 | 0.0012 | 0.0001 | 0.0006 | 0.0004 | 0.0078 | 0.0007 | 0.0041 | 0.0044 | 0.003 | 0.0014 |
Bud Periphery | 0.0051 | 0.0088 | 0.0051 | 0.0051 | 0.0055 | 0.0026 | 0.0016 | 0.0014 | 0.0144 | 0.0003 | 0.044 | 0.0003 | 0.004 | 0.0012 | 0.0052 | 0.0021 | 0.0145 | 0.0005 |
Bud Site | 0.004 | 0.0107 | 0.0068 | 0.0037 | 0.0085 | 0.0014 | 0.0009 | 0.0137 | 0.01 | 0.0003 | 0.0026 | 0.0005 | 0.008 | 0.0138 | 0.0046 | 0.0079 | 0.0136 | 0.0005 |
Cell Periphery | 0.0005 | 0.0004 | 0.0003 | 0.0002 | 0.0004 | 0.0002 | 0.0002 | 0.0006 | 0.0011 | 0.0001 | 0.0006 | 0.0002 | 0.0006 | 0.0005 | 0.0007 | 0.0011 | 0.0008 | 0.0002 |
Cytoplasm | 0.1136 | 0.1056 | 0.0795 | 0.1503 | 0.1261 | 0.1546 | 0.0357 | 0.0474 | 0.0185 | 0.0483 | 0.0156 | 0.1259 | 0.1177 | 0.1696 | 0.1314 | 0.1068 | 0.0872 | 0.2518 |
Cytoplasmic Foci | 0.0474 | 0.0224 | 0.0401 | 0.0795 | 0.0667 | 0.0424 | 0.0193 | 0.012 | 0.031 | 0.0344 | 0.0592 | 0.0223 | 0.054 | 0.0229 | 0.0236 | 0.0934 | 0.0449 | 0.0528 |
Eisosomes | 0.0003 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0002 | 0.0013 | 0.0003 | 0.0004 | 0.0001 |
Endoplasmic Reticulum | 0.0029 | 0.0013 | 0.0021 | 0.0021 | 0.0014 | 0.0018 | 0.0015 | 0.001 | 0.0012 | 0.0005 | 0.0007 | 0.0017 | 0.0048 | 0.0028 | 0.0018 | 0.0019 | 0.0064 | 0.0021 |
Endosome | 0.0547 | 0.0194 | 0.0404 | 0.0825 | 0.0665 | 0.0398 | 0.0155 | 0.012 | 0.0544 | 0.0363 | 0.0563 | 0.01 | 0.0437 | 0.0332 | 0.0344 | 0.0825 | 0.0963 | 0.0381 |
Golgi | 0.0043 | 0.0007 | 0.0019 | 0.009 | 0.0075 | 0.0019 | 0.002 | 0.0003 | 0.004 | 0.0006 | 0.0118 | 0.0015 | 0.0084 | 0.0005 | 0.0012 | 0.01 | 0.0138 | 0.0029 |
Lipid Particles | 0.0094 | 0.0011 | 0.0047 | 0.0044 | 0.0193 | 0.0095 | 0.0054 | 0.0028 | 0.0127 | 0.0018 | 0.0046 | 0.0063 | 0.028 | 0.003 | 0.0084 | 0.0182 | 0.0273 | 0.0144 |
Mitochondria | 0.0093 | 0.0017 | 0.0034 | 0.0089 | 0.0129 | 0.0038 | 0.0027 | 0.0008 | 0.0103 | 0.003 | 0.1669 | 0.0044 | 0.0148 | 0.001 | 0.0028 | 0.0222 | 0.0113 | 0.0036 |
None | 0.6702 | 0.7798 | 0.7515 | 0.5097 | 0.5125 | 0.6681 | 0.8745 | 0.8698 | 0.7366 | 0.8503 | 0.5828 | 0.802 | 0.5765 | 0.6884 | 0.7026 | 0.5477 | 0.5199 | 0.5693 |
Nuclear Periphery | 0.0031 | 0.001 | 0.005 | 0.0018 | 0.0022 | 0.0021 | 0.0013 | 0.0005 | 0.002 | 0.0006 | 0.0009 | 0.0022 | 0.0099 | 0.0011 | 0.0016 | 0.0021 | 0.0095 | 0.0012 |
Nucleolus | 0.0024 | 0.0022 | 0.0018 | 0.0024 | 0.0028 | 0.0011 | 0.0011 | 0.0032 | 0.0033 | 0.0004 | 0.0008 | 0.0006 | 0.0027 | 0.001 | 0.0032 | 0.0051 | 0.0027 | 0.0009 |
Nucleus | 0.0046 | 0.0048 | 0.0097 | 0.0055 | 0.0063 | 0.0041 | 0.0015 | 0.0031 | 0.0043 | 0.0011 | 0.0009 | 0.0054 | 0.0052 | 0.0026 | 0.0047 | 0.0051 | 0.0087 | 0.0023 |
Peroxisomes | 0.0053 | 0.001 | 0.0059 | 0.0079 | 0.0203 | 0.0068 | 0.0011 | 0.0002 | 0.0047 | 0.0007 | 0.004 | 0.0016 | 0.0345 | 0.0007 | 0.0018 | 0.0172 | 0.0098 | 0.0081 |
Punctate Nuclear | 0.0041 | 0.0071 | 0.0091 | 0.0024 | 0.0043 | 0.0042 | 0.0011 | 0.0017 | 0.0141 | 0.0007 | 0.0018 | 0.0013 | 0.0074 | 0.0008 | 0.0065 | 0.0044 | 0.0221 | 0.0047 |
Vacuole | 0.0317 | 0.0215 | 0.0183 | 0.0664 | 0.1054 | 0.0376 | 0.0146 | 0.0203 | 0.0527 | 0.0167 | 0.0205 | 0.0104 | 0.0447 | 0.0521 | 0.0523 | 0.0543 | 0.0788 | 0.0326 |
Vacuole Periphery | 0.003 | 0.0015 | 0.0021 | 0.0069 | 0.0091 | 0.0034 | 0.001 | 0.0008 | 0.016 | 0.0033 | 0.0084 | 0.0008 | 0.0042 | 0.0029 | 0.0046 | 0.0053 | 0.0111 | 0.0039 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 496.3269 | 479.1319 | 557.7933 | 587.5701 | 526.5078 | 484.4366 | 563.2347 | 584.7108 | 566.8887 | 529.8523 |
Translational Efficiency | 1.4711 | 1.4262 | 1.3267 | 1.1873 | 1.1774 | 1.6165 | 1.3513 | 1.201 | 1.3094 | 1.3247 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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