Standard name
Human Ortholog
Description Protein involved in retention of membrane proteins; including Sec12p, in the ER; localized to Golgi; functions as a retrieval receptor in returning membrane proteins to the ER

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.11 0.15 0.08 0 0 0 0 0 0 0 0 0 0.15 0.08 0 0.12 0.12 0.14 0.06 0.16 0.09 0.06 0.08 0.06
Bud 0 0 0 0 0 0 0.05 0.06 0.06 0.06 0.08 0.06 0 0 0 0 0 0.05 0 0 0 0.05 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.46 0.5 0.37 0.5 0.56 0.51 0.46 0.47 0.41 0.51 0.46 0.44 0.46 0.49 0.51 0.6 0.63 0.42 0.07 0.12 0.07 0.07 0.08 0.14
Endoplasmic Reticulum 0.33 0.36 0.26 0.1 0 0 0 0 0 0 0 0 0.28 0.36 0.1 0 0 0.08 0.16 0.17 0.16 0.1 0.12 0.15
Endosome 0.09 0.06 0.1 0.11 0.18 0 0.05 0 0 0 0 0 0.15 0.07 0.26 0.21 0.16 0.25 0.19 0.13 0.18 0.13 0.13 0.09
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.22 0.2 0.21 0.16 0.11 0.08
Mitochondria 0.05 0 0.36 0.29 0.26 0.52 0.59 0.59 0.67 0.54 0.69 0.66 0 0 0 0.08 0 0.09 0.19 0.13 0.2 0.29 0.32 0.3
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.06 0 0.12 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.07 0.08 0.05 0 0 0.07 0.08 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 11 24 11 5 4 4 0 2 0 0 0 3 19 15 4 15 10 14 10 33 14 7 12 11
Bud 2 0 4 8 8 12 27 29 22 34 28 37 1 2 2 1 3 5 1 2 0 6 3 3
Bud Neck 0 0 0 0 0 0 1 0 0 0 0 0 1 1 1 0 0 0 3 3 1 1 0 2
Bud Site 0 0 0 0 0 0 3 2 5 9 2 5 0 0 0 0 0 0
Cell Periphery 2 0 0 0 1 2 0 2 2 1 1 4 0 1 0 2 0 1 0 0 0 0 0 0
Cytoplasm 44 81 48 113 171 148 236 232 154 282 162 266 57 88 66 76 52 43 11 25 11 8 12 25
Endoplasmic Reticulum 32 59 34 23 11 12 11 2 1 3 4 19 34 64 13 5 3 8 27 36 27 11 18 27
Endosome 9 10 13 25 55 8 27 13 4 18 1 0 18 12 34 27 13 26 31 28 29 16 20 16
Golgi 1 5 3 7 0 0 0 0 0 0 0 0 0 9 3 0 1 1 36 42 34 19 17 15
Mitochondria 5 0 47 65 79 151 298 289 249 296 244 404 1 1 1 10 3 9 30 27 32 35 50 54
Nucleus 0 0 0 0 0 0 2 3 1 3 2 4 0 0 0 0 0 0 0 0 1 0 0 0
Nuclear Periphery 0 1 0 0 0 1 1 1 1 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0
Nucleolus 0 0 0 0 2 0 1 1 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 5 5 0 4 4 2 6 0 2 1 0 1 0 0 0 2 1 5 4 2 2
SpindlePole 1 0 1 7 6 3 14 15 15 26 4 21 2 2 4 8 3 12 2 1 1 0 1 1
Vac/Vac Membrane 1 1 0 1 7 0 6 9 4 11 5 5 5 2 11 2 6 8 7 6 2 8 12 15
Unique Cell Count 96 162 130 226 303 290 508 491 373 551 356 608 123 178 130 127 83 103 167 213 165 123 157 180
Labelled Cell Count 108 181 161 259 349 341 631 604 461 689 454 770 139 198 141 146 94 127 167 213 165 123 157 180


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.4 8.1 5.6 5.0 5.3 4.3 3.9 3.8 3.4 3.5 3.2 3.3 7.2 7.2 7.2 7.3 7.8 7.3 7.9 8.0 7.3
Std Deviation (1e-4) 1.0 1.6 1.2 1.3 2.0 1.2 1.3 1.2 0.9 0.9 0.8 0.7 1.5 1.4 1.3 1.2 1.4 1.3 1.8 1.8 1.4
Intensity Change (Log2) -0.16 -0.08 -0.37 -0.53 -0.57 -0.69 -0.69 -0.79 -0.75 0.36 0.38 0.36 0.4 0.49 0.39 0.5 0.52 0.4


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.7 0 0 0 0 0 0 0 0 1.7 0 -1.9 0.9 0.9 1.3
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.4 3.7 2.7 2.0 2.1 0.9 2.9 1.7 1.4 1.5 2.2 2.2 3.7 3.7 0.7
Endoplasmic Reticulum -4.0 -7.0 0 0 0 0 0 0 0 0.3 1.8 -3.4 -5.0 -4.2 -3.6
Endosome 0.3 2.1 0 0 0 0 0 0 0 1.1 -1.0 3.4 2.5 1.2 3.1
Golgi 0 0 0 0 0 0 0 0 0 0 1.2 0 0 0 0
Mitochondria -1.4 -2.1 0 0 0 0 0 0 0 -7.2 -8.5 -7.4 -5.5 -5.5 -4.9
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.6
Vacuole 0 0 0 0 0 0 0 0 0 0 0 3.4 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.4874 3.01 3.0138 2.3508 2.4259 2.9063 3.3991 4.8694 4.0284 4.1966 4.2895 3.9486 1.2437 2.7431 2.3331 2.1607 2.1103 1.9772
Actin 0.0559 0.0117 0.024 0.0387 0.0347 0.0313 0.0613 0.0805 0.062 0.0013 0.0342 0.0287 0.0215 0.0059 0.0158 0.0075 0.0043 0.0155
Bud 0.0006 0.0008 0.0006 0.0004 0.0006 0.0004 0.0005 0.0004 0.0009 0.0005 0.008 0.0005 0.0004 0.0006 0.0005 0.0008 0.0003 0.0015
Bud Neck 0.0014 0.0006 0.0009 0.0007 0.0012 0.0047 0.0022 0.001 0.0064 0.0001 0.0003 0.0035 0.0029 0.0004 0.0008 0.0005 0.0005 0.0019
Bud Periphery 0.0008 0.0006 0.0005 0.0002 0.0006 0.0008 0.0005 0.0005 0.001 0.0001 0.0243 0.0013 0.0006 0.0005 0.0007 0.002 0.0003 0.0006
Bud Site 0.0057 0.0048 0.0056 0.0006 0.0018 0.0009 0.0258 0.0088 0.0035 0.0004 0.0025 0.0006 0.0043 0.0028 0.0027 0.0008 0.0004 0.0005
Cell Periphery 0.0006 0.0004 0.0004 0.0002 0.0003 0.0004 0.0017 0.002 0.0002 0 0.0004 0.0003 0.0005 0.0002 0.0004 0.0006 0.0001 0.0002
Cytoplasm 0.076 0.1815 0.1279 0.0371 0.0301 0.0558 0.0577 0.0474 0.0589 0.0592 0.0405 0.0855 0.0644 0.0646 0.0512 0.0787 0.0483 0.0747
Cytoplasmic Foci 0.1725 0.2028 0.1681 0.138 0.1759 0.1715 0.1671 0.2525 0.202 0.1158 0.1563 0.1677 0.1798 0.1885 0.1727 0.153 0.1973 0.1895
Eisosomes 0.0003 0.0001 0.0002 0.0002 0.0006 0.0006 0.0005 0.0014 0.0001 0 0.0005 0.0002 0.0003 0.0001 0.0002 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0262 0.0229 0.0303 0.0099 0.0305 0.0179 0.023 0.0083 0.0234 0.0122 0.0202 0.0116 0.0138 0.0099 0.0082 0.0285 0.017 0.0093
Endosome 0.2994 0.2836 0.309 0.4887 0.3207 0.2988 0.2151 0.2567 0.2819 0.3835 0.3469 0.2365 0.3285 0.3624 0.34 0.3564 0.2912 0.3367
Golgi 0.2216 0.2092 0.26 0.253 0.2908 0.3257 0.2503 0.2352 0.2861 0.4085 0.1801 0.3366 0.2608 0.2805 0.3173 0.251 0.3023 0.283
Lipid Particles 0.0257 0.0136 0.0097 0.0097 0.0132 0.0178 0.0307 0.0122 0.0154 0.0056 0.0137 0.0295 0.0375 0.0187 0.0187 0.0222 0.0197 0.0167
Mitochondria 0.0215 0.0128 0.0222 0.0126 0.0169 0.0188 0.0115 0.0056 0.0097 0.0054 0.0326 0.0216 0.0218 0.0172 0.0291 0.0587 0.0102 0.0193
None 0.0533 0.0303 0.0206 0.0009 0.0521 0.0249 0.069 0.0678 0.0133 0.0003 0.0894 0.0292 0.0306 0.0279 0.0134 0.007 0.0461 0.0322
Nuclear Periphery 0.0027 0.0022 0.0022 0.0006 0.0045 0.0007 0.0052 0.0008 0.0013 0.0008 0.0098 0.0006 0.0015 0.0006 0.0024 0.0063 0.0214 0.0014
Nucleolus 0.0012 0.0001 0.0001 0 0.0009 0.0001 0.0014 0.0001 0.0005 0 0.0006 0.0001 0.0043 0.0001 0.0001 0.0002 0.0004 0.0001
Nucleus 0.002 0.0006 0.0005 0.0001 0.0015 0.0002 0.0132 0.0002 0.0006 0.0001 0.0018 0.0003 0.0017 0.0004 0.0004 0.0064 0.0015 0.0004
Peroxisomes 0.0179 0.0137 0.0117 0.0031 0.0109 0.0246 0.0535 0.0151 0.0255 0.0014 0.0243 0.0415 0.0181 0.014 0.0194 0.0099 0.0245 0.0106
Punctate Nuclear 0.0086 0.0008 0.0006 0.0002 0.0067 0.0004 0.0017 0.0006 0.0023 0.0003 0.0073 0.0007 0.0023 0.0004 0.0007 0.0024 0.0049 0.0009
Vacuole 0.004 0.005 0.0029 0.0029 0.0027 0.0018 0.0065 0.0021 0.0028 0.0021 0.0032 0.002 0.0027 0.0028 0.0031 0.0044 0.0041 0.0028
Vacuole Periphery 0.0018 0.0019 0.0018 0.0022 0.0028 0.0018 0.0017 0.0009 0.0019 0.0022 0.003 0.0015 0.0016 0.0015 0.0023 0.0025 0.005 0.0021

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 90.4908 48.7099 47.7629 58.4821 61.8166 38.8992 76.5143 66.4816 53.9134 78.4236
Translational Efficiency 1.9373 3.158 2.115 1.456 1.9313 4.4396 1.7812 1.4781 1.6172 1.8059

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Protein involved in retention of membrane proteins; including Sec12p, in the ER; localized to Golgi; functions as a retrieval receptor in returning membrane proteins to the ER
Localization
Cell Percentages ER (28%)
Cell Cycle Regulation No
Subcompartmental Group ER-2

Rer1

Rer1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rer1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available