Standard name
Human Ortholog
Description Low-affinity amino acid permease with broad substrate range; involved in uptake of asparagine, glutamine, and other amino acids; expression regulated by SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); AGP1 has a paralog, GNP1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery – 0 0.24 0.18 0.11 0 – 0.05 0.06 0.09 0 0.1 0.08 0.12 0.14 0 0.06 – 0 0 0 0 0 0
Cytoplasm – 0.61 0.39 0.47 0.16 0.18 – 0.22 0.41 0.28 0.55 0.27 0.11 0.1 0.1 0.39 0.72 – 0.05 0.17 0 0.06 0 0
Endoplasmic Reticulum – 0.36 0.36 0.18 0.62 0.8 – 0.72 0.43 0.6 0.34 0.57 0.81 0.79 0.77 0.14 0.06 – 0.88 0.7 0.84 0.72 0.81 0.8
Endosome – 0 0 0.06 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Golgi – 0 0 0.06 0 0 – 0 0 0 0 0 0 0 0 0.05 0 – 0 0 0 0.08 0.07 0.08
Mitochondria – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.34 0 – 0 0 0 0 0 0
Nucleus – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus – 0 0 0.06 0 0 – 0 0 0 0.06 0.05 0 0 0 0.08 0.11 – 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 0.06 0 0 – 0 0.06 0 0 0 0 0 0 0 0.06 – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 3 10 3 4 4
Bud – 0 1 0 1 0 – 0 1 1 0 2 2 3 5 2 0 – 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery – 3 8 3 5 2 – 9 4 11 3 8 25 51 68 1 1 – 2 3 4 0 2 4
Cytoplasm – 69 13 8 7 26 – 38 28 34 42 22 32 43 47 23 13 – 3 44 5 6 9 9
Endoplasmic Reticulum – 41 12 3 28 113 – 125 29 73 26 47 238 341 363 8 1 – 60 181 213 75 215 261
Endosome – 0 0 1 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1
Golgi – 0 0 1 2 0 – 1 0 0 0 1 1 2 4 3 0 – 0 5 5 8 18 25
Mitochondria – 0 0 0 0 0 – 1 0 1 1 0 0 0 0 20 0 – 0 2 3 3 3 5
Nucleus – 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nuclear Periphery – 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 1 0
Nucleolus – 0 0 1 2 1 – 4 3 3 5 4 0 0 0 5 2 – 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 1 2 2 – 1 4 2 1 2 0 3 3 0 1 – 0 6 1 0 3 4
Unique Cell Count 113 33 17 45 142 174 68 122 77 83 295 430 473 59 18 68 259 253 104 267 327
Labelled Cell Count 115 34 18 47 144 179 69 125 78 86 298 443 490 62 18 68 259 253 104 267 327


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 300.4 265.5 280.5 266.8 220.5 – 201.2 223.2 224.3 220.5 233.3 260.1 234.1 217.0 189.9 220.8 – 229.6 262.0 260.7
Std Deviation (1e-4) – 79.3 75.8 115.2 56.8 63.1 – 57.8 46.8 52.7 51.6 48.7 55.9 53.2 49.7 60.1 85.7 – 43.5 59.2 58.3
Intensity Change (Log2) – – – 0.08 0.01 -0.27 – -0.4 -0.25 -0.24 -0.27 -0.19 -0.03 -0.18 -0.29 -0.48 -0.27 – -0.21 -0.02 -0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
Cell Periphery 0 -1.5 0 – 0 0 0 0 0 0 0 0 0 0 –
Cytoplasm 0.5 -2.4 -2.6 – -2.1 0.2 -1.3 1.5 -1.4 0 0 0 0 2.2 –
Endoplasmic Reticulum -1.4 2.3 4.9 – 3.9 0.6 2.4 -0.3 2.0 5.7 5.6 5.1 -2.5 0 –
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
Mitochondria 0 0 0 – 0 0 0 0 0 0 0 0 3.8 0 –
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 144.1508 148.8272 130.2553 127.5055 124.163 147.6274 105.8801 123.0855 102.0106 109.3579 113.9919 112.5958 54.0825 119.2488 130.1585 99.5426 144.7048 130.1642
Actin 0.0011 0.0002 0.0013 0.0005 0.0004 0.0003 0.0092 0.0011 0.0262 0.0004 0.0186 0.0051 0.0467 0.0209 0.0055 0.0093 0.0003 0.0069
Bud 0.0001 0.0001 0.0004 0 0.0001 0 0.0002 0.001 0.0006 0.0001 0.0001 0 0.0011 0.0036 0.0001 0.0007 0 0.0004
Bud Neck 0.0002 0.0003 0.0014 0.0005 0.0012 0.0012 0.0007 0.0009 0.0011 0.001 0.0023 0.0017 0.0014 0.0008 0.0003 0.004 0.0015 0.0006
Bud Periphery 0.0002 0.0003 0.0011 0.0002 0.0004 0.0002 0.0004 0.0019 0.0019 0.0004 0.0008 0.0003 0.0025 0.0048 0.0001 0.002 0.0001 0.0007
Bud Site 0.0001 0.0005 0.0061 0 0.0001 0 0.0018 0.0004 0.005 0.0001 0.0002 0 0.0049 0.007 0.0005 0.005 0 0.0001
Cell Periphery 0.0495 0.1165 0.0781 0.0772 0.0261 0.0423 0.035 0.0693 0.061 0.0614 0.12 0.0246 0.0718 0.1181 0.0591 0.1491 0.0183 0.0379
Cytoplasm 0.0112 0.0186 0.0041 0.0095 0.0054 0.0063 0.0224 0.0125 0.0041 0.0014 0.0022 0.0012 0.0291 0.0029 0.0118 0.1735 0.0015 0.0199
Cytoplasmic Foci 0.0082 0.0001 0.007 0.0002 0.0062 0.0001 0.0231 0.0008 0.0024 0.0015 0.0015 0.0002 0.045 0.0039 0.0024 0.1405 0.0001 0.0013
Eisosomes 0.0027 0.0028 0.0097 0.0076 0.0028 0.0049 0.009 0.0244 0.0096 0.0425 0.0342 0.0076 0.0038 0.0079 0.0045 0.166 0.0003 0.0202
Endoplasmic Reticulum 0.569 0.7375 0.7106 0.6432 0.6768 0.7522 0.394 0.745 0.685 0.5559 0.6055 0.7035 0.1503 0.49 0.686 0.1023 0.783 0.433
Endosome 0.0048 0.0002 0.0017 0.0013 0.0013 0.0004 0.0065 0.0006 0.0158 0.0006 0.0156 0.0032 0.0405 0.0058 0.0207 0.0026 0.0011 0.0178
Golgi 0.0017 0.0005 0.0021 0.0051 0.0016 0.0042 0.0052 0.0021 0.0127 0.0029 0.0108 0.0043 0.0105 0.0099 0.0028 0.0607 0.006 0.0112
Lipid Particles 0.0054 0.0153 0.0124 0.0066 0.0099 0.0041 0.0178 0.004 0.0144 0.0082 0.0125 0.0043 0.0803 0.02 0.0019 0.0271 0.0019 0.0103
Mitochondria 0.0072 0.0008 0.0164 0.002 0.0145 0.0004 0.028 0.0041 0.0146 0.0163 0.0058 0.001 0.0125 0.0493 0.0013 0.0396 0.0002 0.0134
None 0.0041 0.0004 0.0104 0.0017 0.022 0.0011 0.0185 0.0035 0.0012 0.0047 0.0024 0.0007 0.0208 0.0006 0.0022 0.0108 0.0001 0.0017
Nuclear Periphery 0.2815 0.083 0.1091 0.2003 0.1856 0.1652 0.3592 0.1104 0.1243 0.2455 0.1329 0.2254 0.2734 0.1692 0.1775 0.0627 0.1687 0.3487
Nucleolus 0.0149 0.0004 0.0023 0.0009 0.0018 0.0002 0.0108 0.0007 0.0003 0.0111 0.0018 0.0002 0.031 0.0013 0.0002 0.005 0 0.0012
Nucleus 0.0082 0.0018 0.0015 0.0037 0.0019 0.003 0.0164 0.0018 0.0021 0.004 0.0048 0.0023 0.0221 0.0021 0.0065 0.0012 0.0011 0.0045
Peroxisomes 0.0057 0 0.0008 0 0.001 0 0.0024 0.0001 0.0007 0.0004 0.0014 0 0.0018 0.0013 0.0003 0.0146 0 0.0002
Punctate Nuclear 0.0008 0.0001 0.0005 0.0003 0.0006 0.0001 0.0077 0.0002 0.0005 0.0002 0.0038 0.0006 0.0195 0.0001 0.0027 0.0029 0.0001 0.0005
Vacuole 0.015 0.0196 0.0152 0.0222 0.0264 0.0104 0.0155 0.0108 0.0108 0.0127 0.0181 0.0056 0.0862 0.0645 0.0086 0.0073 0.0075 0.0165
Vacuole Periphery 0.0086 0.0012 0.0077 0.0169 0.0138 0.0036 0.0164 0.0044 0.0055 0.0286 0.0048 0.0081 0.0449 0.0159 0.0049 0.0132 0.0083 0.053

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.0224 15.0394 25.0135 28.8481 34.2234 14.4952 15.0752 16.8105 23.4614 22.916
Translational Efficiency 1.029 0.7588 0.8531 1.1599 0.7799 0.6655 0.6497 0.68 0.8724 0.818

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Low-affinity amino acid permease with broad substrate range; involved in uptake of asparagine, glutamine, and other amino acids; expression regulated by SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); AGP1 has a paralog, GNP1, that arose from the whole genome duplication
Localization
Cell Percentages ER (70%)
Cell Cycle Regulation No
Subcompartmental Group ER-4

Agp1

Agp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Agp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available