Standard name | |
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Human Ortholog | |
Description | [PIN(+)] prion; an infectious protein conformation that is generally an ordered protein aggregate |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 |
Bud | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 3 | 3 | 2 | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 2 | 1 | 1 | 3 | 4 | 5 | 4 | 6 | 3 | 4 | 0 | 0 | 1 | 3 | 5 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 171 | 145 | 142 | 99 | 130 | 146 | 150 | 165 | 136 | 144 | 76 | 119 | 190 | 228 | 259 | 264 | 243 | 190 | 169 | 132 | 141 | 80 | 145 | 173 |
Endoplasmic Reticulum | 0 | 0 | 1 | 1 | 0 | 0 | 3 | 13 | 3 | 9 | 2 | 4 | 0 | 1 | 2 | 9 | 5 | 4 | 0 | 0 | 0 | 1 | 0 | 0 |
Endosome | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 1 | 0 | 1 | 4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 1 | 1 | 0 | 0 | 5 | 17 | 11 | 25 | 36 | 15 | 15 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 1 | 0 | 1 | 1 | 0 | 1 | 4 | 6 | 6 | 0 | 4 | 6 | 4 | 8 | 6 | 2 | 1 | 1 | 1 | 5 | 1 | 9 | 4 | 3 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1 | 1 | 0 | 1 | 2 | 1 | 1 | 2 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | 8 | 19 | 16 | 15 | 36 | 29 | 51 | 39 | 28 | 28 | 4 | 14 | 10 | 31 | 4 | 21 | 0 | 1 | 0 | 2 | 1 | 0 |
Unique Cell Count | 172 | 146 | 152 | 113 | 145 | 166 | 206 | 220 | 216 | 220 | 120 | 159 | 195 | 238 | 265 | 292 | 251 | 209 | 174 | 146 | 150 | 97 | 158 | 186 |
Labelled Cell Count | 173 | 147 | 158 | 122 | 147 | 171 | 219 | 235 | 231 | 240 | 130 | 179 | 199 | 252 | 280 | 311 | 264 | 228 | 174 | 146 | 150 | 97 | 158 | 186 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 12.5 | 16.0 | 15.1 | 13.1 | 13.6 | 12.3 | 11.9 | 12.4 | 11.4 | 11.9 | 10.9 | 11.0 | 16.1 | 15.7 | 14.5 | 23.7 | 23.8 | 22.1 | 13.4 | 15.6 | 17.8 |
Std Deviation (1e-4) | 2.4 | 3.4 | 2.8 | 2.8 | 2.7 | 2.7 | 2.3 | 3.0 | 2.4 | 3.1 | 2.6 | 3.0 | 3.1 | 3.2 | 2.9 | 7.5 | 6.9 | 7.0 | 3.1 | 3.1 | 3.5 |
Intensity Change (Log2) | – | – | – | -0.2 | -0.15 | -0.3 | -0.35 | -0.28 | -0.41 | -0.34 | -0.47 | -0.46 | 0.09 | 0.06 | -0.06 | 0.65 | 0.66 | 0.55 | -0.17 | 0.05 | 0.24 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.6 | -1.2 | -1.7 | -5.0 | -4.6 | -6.7 | -6.3 | -6.2 | -4.5 | 1.8 | 1.0 | 2.2 | -1.1 | 1.6 | -0.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 3.3 | 0 | 4.0 | 5.0 | 4.1 | 3.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 3.1 | 1.8 | 1.3 | 3.5 | 2.5 | 4.7 | 3.6 | 4.4 | 3.4 | -1.6 | 0.3 | -0.7 | 1.9 | 0 | 1.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 9.4437 | 10.7552 | 9.7332 | 10.056 | 8.4884 | 10.642 | 7.2842 | 10.0442 | 9.5801 | 8.6108 | 8.3553 | 8.9155 | 8.9851 | 10.8473 | 9.6721 | 8.7845 | 8.2566 | 8.9567 |
Actin | 0.0233 | 0.0011 | 0.0099 | 0.0003 | 0.014 | 0.0004 | 0.0491 | 0.0004 | 0.0006 | 0.0002 | 0.0096 | 0.0003 | 0.0019 | 0.0012 | 0.0008 | 0.0002 | 0.0006 | 0.0016 |
Bud | 0.0003 | 0.0002 | 0.0004 | 0.0001 | 0.0004 | 0.0004 | 0.0029 | 0.0001 | 0.0005 | 0.0002 | 0.0004 | 0.0001 | 0.0009 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0008 |
Bud Neck | 0.0013 | 0.0004 | 0.0006 | 0.0008 | 0.0008 | 0.0017 | 0.0066 | 0.0001 | 0.0002 | 0.0005 | 0.0007 | 0.0007 | 0.0003 | 0.0003 | 0.0006 | 0.0006 | 0.0009 | 0.0011 |
Bud Periphery | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0034 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0004 | 0 | 0 | 0.0002 |
Bud Site | 0.0007 | 0.0046 | 0.0013 | 0.0001 | 0.0005 | 0.0004 | 0.038 | 0.0006 | 0.0002 | 0.0001 | 0.0007 | 0 | 0.0008 | 0.0022 | 0.0004 | 0.0001 | 0.0001 | 0.0001 |
Cell Periphery | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.8479 | 0.8936 | 0.8748 | 0.9258 | 0.785 | 0.8727 | 0.6745 | 0.9879 | 0.9796 | 0.9852 | 0.7892 | 0.964 | 0.9148 | 0.9515 | 0.9169 | 0.9047 | 0.9304 | 0.8975 |
Cytoplasmic Foci | 0.0161 | 0.0292 | 0.0074 | 0.0009 | 0.0074 | 0.0041 | 0.0565 | 0.0008 | 0.0008 | 0.0019 | 0.0603 | 0.0012 | 0.0109 | 0.0055 | 0.008 | 0.0187 | 0.0031 | 0.0042 |
Eisosomes | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 | 0.0005 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0064 | 0.001 | 0.0027 | 0.0009 | 0.0082 | 0.0027 | 0.0114 | 0.0004 | 0.0008 | 0.0003 | 0.0011 | 0.0005 | 0.0024 | 0.0008 | 0.0013 | 0.0013 | 0.0014 | 0.0017 |
Endosome | 0.0138 | 0.0025 | 0.0052 | 0.0005 | 0.0138 | 0.002 | 0.0531 | 0.0001 | 0.0002 | 0.0005 | 0.0487 | 0.0004 | 0.0076 | 0.0013 | 0.0076 | 0.0073 | 0.0075 | 0.004 |
Golgi | 0.0042 | 0.0005 | 0.0011 | 0 | 0.0102 | 0.0005 | 0.0219 | 0 | 0 | 0 | 0.0633 | 0 | 0.0012 | 0.0002 | 0.0011 | 0.0003 | 0.0007 | 0.0009 |
Lipid Particles | 0.0061 | 0.0003 | 0.0015 | 0 | 0.0087 | 0.0001 | 0.0188 | 0 | 0 | 0 | 0.0049 | 0 | 0.0009 | 0 | 0.0018 | 0.0012 | 0.0031 | 0.0009 |
Mitochondria | 0.0025 | 0.0201 | 0.0019 | 0.0001 | 0.0021 | 0.0068 | 0.0073 | 0.0001 | 0.0001 | 0.0001 | 0.0022 | 0.0001 | 0.0014 | 0.0002 | 0.0011 | 0.0001 | 0.0005 | 0.0027 |
None | 0.0041 | 0.0121 | 0.011 | 0.0011 | 0.0462 | 0.0156 | 0.0061 | 0.0013 | 0.0013 | 0.0009 | 0.0005 | 0.0008 | 0.0055 | 0.0013 | 0.0016 | 0.0018 | 0.0021 | 0.0015 |
Nuclear Periphery | 0.0093 | 0.002 | 0.0085 | 0.0029 | 0.0083 | 0.0038 | 0.009 | 0.0006 | 0.001 | 0.0007 | 0.0015 | 0.0015 | 0.0068 | 0.0018 | 0.0033 | 0.0029 | 0.0118 | 0.0084 |
Nucleolus | 0.0002 | 0.0027 | 0.0003 | 0 | 0.0001 | 0.0046 | 0.0006 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0009 | 0.0001 |
Nucleus | 0.0505 | 0.0229 | 0.065 | 0.0654 | 0.0849 | 0.0795 | 0.0125 | 0.0072 | 0.0141 | 0.0085 | 0.0086 | 0.0295 | 0.0384 | 0.0306 | 0.05 | 0.0578 | 0.0288 | 0.0693 |
Peroxisomes | 0.0076 | 0.0014 | 0.0016 | 0 | 0.0034 | 0.0001 | 0.0144 | 0 | 0 | 0.0001 | 0.0062 | 0 | 0.0027 | 0.0006 | 0.0024 | 0.0004 | 0.0003 | 0.002 |
Punctate Nuclear | 0.0029 | 0.0051 | 0.0051 | 0.0007 | 0.0027 | 0.0035 | 0.0045 | 0.0002 | 0.0002 | 0.0001 | 0.0009 | 0.0005 | 0.0017 | 0.0016 | 0.001 | 0.002 | 0.0065 | 0.002 |
Vacuole | 0.0016 | 0.0002 | 0.0011 | 0.0003 | 0.0023 | 0.0006 | 0.0054 | 0.0001 | 0.0002 | 0.0004 | 0.0004 | 0.0002 | 0.0008 | 0.0004 | 0.001 | 0.0004 | 0.0007 | 0.0005 |
Vacuole Periphery | 0.0005 | 0.0001 | 0.0004 | 0.0001 | 0.0005 | 0.0002 | 0.0017 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0007 | 0.0001 | 0.0003 | 0.0001 | 0.0004 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 59.3374 | 59.1178 | 44.4689 | 63.4016 | 76.9336 | 57.041 | 65.301 | 64.3836 | 56.686 | 69.451 |
Translational Efficiency | 3.1714 | 2.8564 | 3.5359 | 1.9821 | 2.4367 | 3.7919 | 2.096 | 2.4502 | 2.5965 | 2.623 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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