Standard name
Human Ortholog
Description Adaptor protein for various signaling pathways; involved in mating response, invasive/filamentous growth, osmotolerance; acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – – 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0.14 0.14 0.13 0 0 0 0 0 0
Cytoplasm 0.98 0.98 – – 1.0 0.88 0.91 0.93 0.76 0.86 0.71 0.67 1.0 0.99 0.99 0.92 0.96 0.97 0.88 0.92 0.72 0.89 0.86 0.88
Endoplasmic Reticulum 0 0 – – 0 0 0 0 0 0 0 0.05 0 0 0 0 0.07 0 0 0 0 0 0 0
Endosome 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 – – 0 0.29 0.19 0.11 0.39 0.18 0.54 0.38 0 0 0 0 0 0 0 0 0.14 0 0.06 0
Nucleus 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 0 – – 0 0 0 1 0 0 0 0 1 0 0 3 0 0 1 0 0 1 1 6
Bud 1 2 – – 0 1 4 1 4 2 5 2 2 0 0 0 0 1 0 0 0 0 2 4
Bud Neck 0 0 – – 0 0 0 1 0 2 0 0 0 0 1 0 0 0 3 1 0 1 2 2
Bud Site 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 – – 1 0 2 5 0 4 2 1 4 6 3 30 23 20 0 0 0 0 1 1
Cytoplasm 276 140 – – 37 43 145 145 90 124 45 44 222 186 248 197 155 146 258 135 13 125 208 277
Endoplasmic Reticulum 3 3 – – 0 0 0 2 2 4 1 3 2 1 0 8 11 4 5 1 0 2 3 5
Endosome 0 0 – – 0 0 1 1 0 0 1 0 0 0 1 7 0 0 3 0 0 0 2 0
Golgi 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 2 1 0 2 0 0 0 0 0
Mitochondria 16 1 – – 0 14 31 17 46 26 34 25 0 0 0 1 5 0 9 2 2 6 13 3
Nucleus 1 0 – – 0 2 0 1 0 1 0 1 0 0 0 0 1 1 0 0 0 0 0 0
Nuclear Periphery 0 0 – – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 – – 0 1 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – – 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Vac/Vac Membrane 0 1 – – 0 1 1 0 0 2 0 0 0 1 0 11 2 0 6 4 0 1 3 3
Unique Cell Count 283 143 37 49 159 156 118 144 63 66 222 188 251 214 161 151 294 147 19 142 243 317
Labelled Cell Count 300 148 38 63 185 174 143 165 88 76 231 194 255 259 198 172 294 147 19 142 243 317


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 6.1 – – 5.8 4.4 4.2 4.8 4.0 4.2 4.1 4.1 6.1 6.3 6.4 8.9 8.2 8.4 5.6 6.1 6.4
Std Deviation (1e-4) 0.5 0.8 – – 0.8 0.7 0.5 0.6 0.5 0.6 0.5 0.5 0.8 0.9 1.3 1.6 1.2 1.3 1.1 1.3 1.7
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.4851 2.251 1.8969 1.8014 1.7859 2.0127 1.242 1.8875 1.6699 1.7823 1.8098 1.7434 2.5521 1.1839 1.3838 0.4533 0.2904 0.5885
Actin 0.0068 0.0204 0.0098 0.0043 0.0272 0.0124 0.0271 0.0004 0.0142 0.0319 0.0169 0.0066 0.0376 0.0006 0.0137 0.1467 0.0046 0.013
Bud 0.0033 0.0006 0.0012 0.0015 0.0017 0.0008 0.0046 0.0086 0.0025 0.0016 0.0072 0.0066 0.0014 0.0001 0.0006 0.0018 0.0001 0.0002
Bud Neck 0.0025 0.0006 0.0022 0.0008 0.0007 0.0118 0.0022 0.0004 0.0019 0.0024 0.002 0.0049 0.0019 0.0001 0.0003 0.0073 0.0002 0.0011
Bud Periphery 0.0052 0.0007 0.0019 0.0022 0.0035 0.0026 0.0054 0.0015 0.0019 0.002 0.0095 0.0083 0.0027 0.0001 0.0004 0.0024 0 0.0004
Bud Site 0.01 0.0068 0.0067 0.0011 0.0023 0.0005 0.0062 0.0312 0.0098 0.0106 0.0093 0.0015 0.0062 0.0018 0.0022 0.0066 0.0001 0.0004
Cell Periphery 0.0015 0.0008 0.0005 0.0003 0.0002 0.0002 0.0009 0.0004 0.0005 0.0005 0.0007 0.0005 0.0019 0.0003 0.0006 0.0008 0.0001 0.0002
Cytoplasm 0.6327 0.7963 0.7196 0.616 0.5709 0.7762 0.5524 0.7 0.6955 0.6474 0.6545 0.7381 0.6226 0.8266 0.7729 0.4157 0.7573 0.7935
Cytoplasmic Foci 0.0319 0.0137 0.0266 0.0118 0.0523 0.0162 0.0308 0.026 0.0245 0.0438 0.028 0.0256 0.0358 0.0165 0.0155 0.052 0.056 0.0275
Eisosomes 0.0021 0.0005 0.0003 0.0001 0.0003 0.0001 0.0005 0 0.0001 0.0003 0.0003 0.0002 0.0007 0.0001 0.0011 0.0007 0 0
Endoplasmic Reticulum 0.0051 0.002 0.0024 0.0032 0.0014 0.0025 0.0111 0.0019 0.0038 0.0051 0.0043 0.0032 0.0117 0.0046 0.0056 0.0127 0.0074 0.0087
Endosome 0.0307 0.0105 0.0205 0.0183 0.0575 0.0138 0.0456 0.0175 0.0249 0.0473 0.0622 0.0165 0.0297 0.0076 0.0147 0.132 0.0068 0.0386
Golgi 0.0085 0.0047 0.0055 0.004 0.0378 0.0034 0.0067 0.0014 0.0038 0.0053 0.0097 0.0026 0.0108 0.001 0.0022 0.0668 0.0016 0.0229
Lipid Particles 0.0089 0.0003 0.0015 0.0004 0.0147 0.0007 0.009 0.0031 0.0039 0.0175 0.0164 0.0024 0.0153 0.0004 0.0095 0.0149 0.0083 0.0026
Mitochondria 0.0164 0.0046 0.0079 0.0302 0.0489 0.0027 0.007 0.0004 0.0039 0.0041 0.0085 0.0062 0.051 0.0001 0.0314 0.0358 0.0003 0.0302
None 0.2029 0.1327 0.173 0.2856 0.1053 0.1507 0.2545 0.1919 0.1837 0.0955 0.0738 0.1615 0.0935 0.1358 0.1129 0.0485 0.0939 0.0461
Nuclear Periphery 0.0033 0.0003 0.0006 0.0016 0.0006 0.0003 0.0067 0.0007 0.0025 0.0083 0.0253 0.0011 0.0196 0.0006 0.0008 0.0034 0.001 0.0007
Nucleolus 0.0012 0.0001 0.0003 0.0002 0.0003 0 0.0015 0.0003 0.0008 0.0021 0.0017 0.0015 0.0022 0.0001 0.0002 0.0003 0 0.0001
Nucleus 0.0027 0.0009 0.0013 0.0016 0.0007 0.0005 0.0064 0.0023 0.0031 0.006 0.0363 0.0022 0.0043 0.0008 0.0009 0.001 0.0013 0.0006
Peroxisomes 0.0124 0.0015 0.0107 0.0007 0.0642 0.0024 0.0045 0.0048 0.0069 0.0447 0.0053 0.0051 0.0141 0.0002 0.0087 0.0378 0.058 0.0082
Punctate Nuclear 0.0045 0.0003 0.0008 0.0009 0.0008 0.0003 0.0078 0.0034 0.0027 0.0098 0.0178 0.0024 0.0055 0.0006 0.0004 0.0013 0.0009 0.0005
Vacuole 0.0062 0.0016 0.0058 0.0097 0.0064 0.0013 0.0077 0.0032 0.008 0.0118 0.0078 0.0025 0.0164 0.0019 0.0047 0.0086 0.0019 0.0031
Vacuole Periphery 0.001 0.0003 0.0009 0.0054 0.0022 0.0003 0.0015 0.0004 0.0011 0.0017 0.0024 0.0006 0.0152 0.0002 0.0006 0.0026 0.0003 0.0012

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.6594 1.8951 10.5466 11.1892 13.0555 14.831 14.8497 16.812 13.1636 12.5102
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –
Standard name
Human Ortholog
Description Adaptor protein for various signaling pathways; involved in mating response, invasive/filamentous growth, osmotolerance; acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction
Localization
Cell Percentages cytoplasm (94%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ste50

Ste50


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ste50-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available