Standard name
Human Ortholog
Description Subunit of GID Complex that binds directly to central component Vid30p; GID complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; Gid7p contains six WD40 repeats; computational analysis suggests that Gid7p and Moh1p have similar functions
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.6 0.59 0.81 0.78 0.68 0.58 0.57 0.56 0.42 – 0.39 0.5 0.54 0.5 0.53 0.58 0.48 0.52 0.56 0.44 0.56 0.44 0.39 0.37
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05
Endosome 0.05 0 0 0 0 0 0 0 0 – 0 0 0 0.08 0.15 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0.11 0 0.07 0.05 0.22 0.19 0.11 0.5 – 0.6 0.3 0.06 0 0 0 0 0 0.05 0 0.1 0.09 0.11 0.08
Nucleus 0 0 0 0 0.06 0.09 0.12 0.15 0.08 – 0.07 0.14 0 0 0 0.06 0.06 0.05 0.11 0.17 0.07 0.05 0.06 0.05
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.36 0.37 0.18 0.14 0.28 0.2 0.26 0.25 0.26 – 0.16 0.21 0.46 0.55 0.5 0.51 0.55 0.5 0.13 0.24 0.16 0.24 0.29 0.29
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 1 0 – 0 2 0 0 0 0 1 0 0 0 2 0 3 3
Bud 0 1 1 3 1 1 4 5 9 – 2 6 2 1 1 0 0 0 1 1 1 5 7 12
Bud Neck 0 0 2 0 0 1 0 1 0 – 0 0 0 0 0 0 0 0 2 2 2 1 3 6
Bud Site 0 0 0 0 1 1 2 0 0 – 0 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 1 1 0 0 0 – 3 3 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 106 58 151 116 144 140 232 210 123 – 105 152 164 180 237 151 78 104 126 69 146 71 146 137
Endoplasmic Reticulum 5 1 3 1 5 0 2 11 3 – 1 7 4 3 1 11 1 7 3 0 2 6 17 18
Endosome 8 0 1 2 4 4 8 4 2 – 0 4 11 30 67 6 4 5 7 4 3 3 9 12
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 2 1 1 0 0 0 1 2
Mitochondria 17 11 1 11 11 52 75 41 144 – 162 91 17 4 5 2 2 1 10 3 25 13 40 28
Nucleus 2 2 6 6 13 21 47 54 22 – 19 43 4 7 11 16 9 11 23 26 19 8 22 19
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 1 0 0 1 0 0 0 1 4 0 0 0 1 2
Nucleolus 0 0 0 0 0 0 0 0 0 – 1 1 0 0 0 0 0 0 1 1 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 2 0 0 0 – 0 1 0 0 1 0 0 0 9 5 7 4 6 12
Vac/Vac Membrane 63 37 34 21 60 48 104 94 75 – 44 64 138 199 224 132 89 101 29 36 43 39 111 108
Unique Cell Count 176 99 187 148 212 241 405 372 290 268 306 303 359 451 260 161 201 224 156 264 161 379 375
Labelled Cell Count 201 110 200 161 240 271 474 421 378 338 376 340 425 547 319 186 231 224 156 264 161 379 375


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 5.1 5.3 4.9 5.9 4.9 5.3 5.7 4.7 – 4.5 5.4 5.2 5.7 5.8 9.6 9.8 10.0 4.7 4.8 5.4
Std Deviation (1e-4) 0.6 0.8 0.9 0.9 1.2 0.9 1.0 1.1 1.0 – 0.9 1.0 0.8 1.0 1.0 1.3 1.8 1.8 1.1 1.1 1.3
Intensity Change (Log2) – – – -0.12 0.15 -0.12 -0.0 0.09 -0.19 – -0.25 0.0 -0.03 0.09 0.12 0.84 0.88 0.91 -0.19 -0.14 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm -0.5 -2.9 -5.0 -5.6 -5.7 -8.3 – -8.8 -6.9 -6.0 -6.9 -6.6 -5.1 -6.3 -6.0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 1.6 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 3.7 5.3 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 3.4 2.7 6.6 6.1 4.4 0 – 0 8.1 2.9 0 0 0 0 0
Nucleus 0.4 1.4 2.3 3.3 4.1 2.0 – 1.8 3.9 0 0 0 1.4 1.1 1.1
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole -1.0 2.4 0.5 2.0 1.9 2.0 – -0.5 0.7 6.2 8.4 7.4 7.0 7.2 6.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.5633 1.1289 0.885 1.0323 0.6472 1.0384 0.8064 1.0364 0.8391 0.676 0.7827 1.0026 0.8515 1.4433 0.8748 0.7897 0.8692 1.0429
Actin 0.0339 0.0032 0.0147 0.0257 0.0091 0.0011 0.0352 0.0003 0.0021 0.0058 0.004 0.0036 0.0068 0.0016 0.0055 0.0011 0.0156 0.0016
Bud 0.0007 0.0002 0.0003 0.0002 0.001 0.0004 0.0008 0.0002 0.0006 0.0006 0.0041 0.0006 0.0005 0.0009 0.0004 0.0005 0.0008 0.0011
Bud Neck 0.0034 0.0004 0.0012 0.0012 0.0066 0.0047 0.001 0.0005 0.0012 0.0017 0.0026 0.0057 0.0035 0.0005 0.0007 0.0049 0.0012 0.005
Bud Periphery 0.0007 0.0001 0.0002 0.0004 0.001 0.0003 0.0011 0.0001 0.0005 0.0008 0.0053 0.0009 0.0005 0.0006 0.0002 0.0003 0.0009 0.0013
Bud Site 0.0014 0.0015 0.0013 0.0006 0.0028 0.0006 0.002 0.0012 0.0024 0.0009 0.0061 0.0016 0.0017 0.0024 0.0018 0.0014 0.0014 0.0005
Cell Periphery 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0 0.0009 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0
Cytoplasm 0.4737 0.5644 0.5384 0.4933 0.4439 0.5403 0.404 0.6146 0.5437 0.4061 0.3977 0.5127 0.4235 0.6086 0.4465 0.4249 0.403 0.4553
Cytoplasmic Foci 0.0209 0.0084 0.0075 0.0052 0.0119 0.0129 0.0276 0.0064 0.0096 0.0161 0.0243 0.0135 0.0371 0.0111 0.0107 0.0106 0.0269 0.0093
Eisosomes 0.0002 0 0.0001 0.0017 0.0001 0 0.0002 0 0 0 0.0001 0 0.0001 0 0.0001 0 0.0002 0
Endoplasmic Reticulum 0.0157 0.0177 0.0152 0.0035 0.0111 0.0103 0.0163 0.0079 0.01 0.0048 0.0073 0.0064 0.0105 0.0078 0.0046 0.0127 0.0083 0.0046
Endosome 0.033 0.0085 0.0106 0.0057 0.0237 0.0192 0.0586 0.0118 0.0203 0.0134 0.0281 0.0184 0.0275 0.0065 0.0183 0.0156 0.0514 0.0132
Golgi 0.0098 0.0037 0.001 0.0059 0.002 0.0009 0.0186 0.0003 0.0059 0.0069 0.0219 0.0046 0.0056 0.0013 0.0025 0.0004 0.0146 0.0025
Lipid Particles 0.0044 0.0017 0.0007 0.0326 0.0011 0.0011 0.0163 0.0004 0.0014 0.0018 0.0496 0.0025 0.0074 0.001 0.0015 0.0022 0.0128 0.0027
Mitochondria 0.0103 0.0043 0.001 0.0237 0.0014 0.0014 0.0141 0.0005 0.0024 0.0132 0.0744 0.0028 0.0075 0.0008 0.0115 0.0007 0.0483 0.0022
None 0.1147 0.1588 0.1202 0.1407 0.1257 0.0813 0.1019 0.0751 0.0949 0.058 0.1309 0.0422 0.0879 0.0588 0.0787 0.1003 0.0324 0.0404
Nuclear Periphery 0.0277 0.0167 0.0235 0.009 0.0224 0.0132 0.049 0.0185 0.0239 0.0121 0.0169 0.0138 0.0371 0.0172 0.016 0.0311 0.0194 0.0186
Nucleolus 0.0013 0.0005 0.0016 0.0006 0.0019 0.0014 0.0017 0.0006 0.0009 0.0008 0.0026 0.0018 0.0009 0.0008 0.0013 0.0013 0.0007 0.001
Nucleus 0.2202 0.1961 0.2428 0.194 0.3109 0.282 0.2097 0.2501 0.254 0.4044 0.1784 0.3398 0.3011 0.2554 0.3746 0.3442 0.3404 0.4217
Peroxisomes 0.002 0.0044 0.0003 0.0458 0.0009 0.0003 0.0109 0.0002 0.0009 0.0048 0.0143 0.0024 0.017 0.0054 0.0022 0.0003 0.0051 0.0016
Punctate Nuclear 0.0195 0.0058 0.0152 0.0072 0.0142 0.0216 0.0142 0.0069 0.0189 0.0446 0.0165 0.0207 0.0172 0.0154 0.0194 0.0423 0.0112 0.0135
Vacuole 0.0051 0.0029 0.0034 0.0024 0.0073 0.0061 0.0098 0.0038 0.005 0.0023 0.0113 0.0045 0.0051 0.0034 0.0027 0.0042 0.0035 0.0028
Vacuole Periphery 0.0013 0.0003 0.0007 0.0006 0.0011 0.0011 0.0066 0.0006 0.0012 0.0006 0.0026 0.0013 0.0015 0.0005 0.0006 0.0008 0.0017 0.0011

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.6281 6.1704 7.5221 10.1213 9.9082 12.319 12.9233 16.2417 13.4235 11.245
Translational Efficiency 0.7548 1.0875 0.8002 0.6503 0.6074 0.6743 0.6847 0.5004 0.7103 0.658

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of GID Complex that binds directly to central component Vid30p; GID complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; Gid7p contains six WD40 repeats; computational analysis suggests that Gid7p and Moh1p have similar functions
Localization
Cell Percentages cytoplasm (46%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Gid7

Gid7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gid7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available