Standard name | |
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Human Ortholog | |
Description | Subunit of the mitochondrial (mt) i-AAA protease supercomplex; i-AAA degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr3p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 14 | 4 | 8 | 3 | 10 | 6 | 4 | 1 | 3 | 0 | 11 | 11 |
Bud | 4 | 0 | 5 | 6 | 10 | 6 | 21 | 26 | 22 | 28 | 16 | 22 | 6 | 4 | 4 | 5 | 12 | 13 | 1 | 0 | 1 | 0 | 8 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 0 | 3 | 0 | 2 | 1 | 8 | 10 |
Bud Site | 0 | 0 | 1 | 2 | 1 | 2 | 11 | 10 | 9 | 8 | 7 | 6 | 0 | 0 | 0 | 0 | 1 | 4 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 7 | 9 | 4 | 1 | 2 | 2 | 5 | 1 | 2 | 5 | 9 | 8 | 11 | 14 | 17 | 12 | 0 | 0 | 0 | 0 | 1 | 1 |
Cytoplasm | 6 | 4 | 19 | 14 | 16 | 17 | 17 | 20 | 10 | 20 | 5 | 15 | 131 | 183 | 284 | 30 | 29 | 32 | 1 | 3 | 0 | 4 | 23 | 14 |
Endoplasmic Reticulum | 6 | 2 | 1 | 0 | 2 | 2 | 1 | 2 | 2 | 0 | 0 | 1 | 9 | 15 | 8 | 13 | 11 | 17 | 2 | 0 | 1 | 0 | 3 | 3 |
Endosome | 7 | 0 | 43 | 8 | 37 | 9 | 11 | 11 | 0 | 1 | 2 | 1 | 48 | 53 | 93 | 32 | 32 | 42 | 22 | 5 | 27 | 7 | 27 | 23 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 4 | 10 | 29 | 24 | 3 | 0 | 8 | 0 | 6 | 2 |
Mitochondria | 200 | 38 | 192 | 201 | 268 | 306 | 453 | 566 | 418 | 618 | 258 | 405 | 83 | 37 | 47 | 25 | 26 | 32 | 183 | 57 | 206 | 105 | 362 | 306 |
Nucleus | 6 | 0 | 1 | 1 | 2 | 11 | 16 | 20 | 9 | 9 | 4 | 4 | 2 | 3 | 2 | 10 | 9 | 9 | 0 | 1 | 0 | 0 | 7 | 9 |
Nuclear Periphery | 5 | 0 | 0 | 3 | 1 | 5 | 5 | 10 | 4 | 8 | 5 | 13 | 1 | 0 | 0 | 3 | 0 | 2 | 0 | 0 | 2 | 1 | 1 | 1 |
Nucleolus | 3 | 0 | 2 | 2 | 2 | 5 | 9 | 11 | 2 | 9 | 4 | 1 | 0 | 0 | 0 | 2 | 2 | 6 | 1 | 1 | 1 | 1 | 3 | 4 |
Peroxisomes | 2 | 0 | 43 | 17 | 28 | 9 | 10 | 12 | 5 | 4 | 2 | 4 | 8 | 17 | 64 | 7 | 8 | 14 | 6 | 0 | 14 | 1 | 5 | 6 |
SpindlePole | 3 | 0 | 22 | 7 | 8 | 10 | 15 | 20 | 16 | 22 | 2 | 10 | 10 | 18 | 18 | 9 | 13 | 11 | 2 | 0 | 3 | 3 | 11 | 9 |
Vac/Vac Membrane | 21 | 3 | 32 | 13 | 36 | 27 | 26 | 41 | 29 | 40 | 20 | 20 | 47 | 73 | 83 | 60 | 68 | 72 | 9 | 8 | 6 | 8 | 42 | 43 |
Unique Cell Count | 215 | 40 | 261 | 221 | 322 | 351 | 494 | 620 | 438 | 642 | 268 | 427 | 328 | 361 | 523 | 191 | 217 | 233 | 246 | 83 | 283 | 140 | 533 | 462 |
Labelled Cell Count | 263 | 47 | 368 | 283 | 415 | 410 | 598 | 751 | 531 | 768 | 327 | 507 | 371 | 420 | 628 | 223 | 267 | 296 | 246 | 83 | 283 | 140 | 533 | 462 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.1 | 4.5 | 4.8 | 4.6 | 4.6 | 4.5 | 4.2 | 4.2 | 4.1 | 4.0 | 3.8 | 4.0 | 6.0 | 5.9 | 5.6 | 6.6 | 7.2 | 7.0 | 5.4 | 5.9 | 6.0 |
Std Deviation (1e-4) | 0.8 | 0.6 | 1.3 | 1.6 | 1.0 | 1.9 | 1.2 | 1.6 | 1.1 | 1.3 | 1.6 | 1.5 | 1.0 | 0.8 | 0.8 | 1.3 | 1.5 | 1.6 | 1.1 | 1.0 | 1.2 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.05 | -0.11 | -0.21 | -0.19 | -0.25 | -0.28 | -0.34 | -0.25 | 0.31 | 0.28 | 0.22 | 0.45 | 0.58 | 0.55 | 0.18 | 0.29 | 0.32 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 5.0 | 5.1 | 5.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.3461 | 2.5773 | 2.5166 | 1.8684 | 1.6902 | 2.5033 | 0.4224 | 0.8445 | 0.5853 | 0.3343 | 0.0966 | 0.4294 | -0.0852 | 0.3816 | 0.1592 | 0.3483 | -0.0705 | 0.2008 |
Actin | 0.0064 | 0.0867 | 0.0152 | 0.0015 | 0.0005 | 0.0109 | 0.0522 | 0.0207 | 0.013 | 0.0012 | 0.0274 | 0.0097 | 0.0157 | 0.0168 | 0.0246 | 0.0196 | 0.0043 | 0.0044 |
Bud | 0.0007 | 0.0012 | 0.0044 | 0.0011 | 0.0023 | 0.0007 | 0.0023 | 0.0044 | 0.0071 | 0.0005 | 0.0007 | 0.0021 | 0.0017 | 0.0042 | 0.005 | 0.0058 | 0.0033 | 0.0024 |
Bud Neck | 0.0003 | 0.0016 | 0.002 | 0.0056 | 0.0002 | 0.0145 | 0.0032 | 0.0011 | 0.0025 | 0.0003 | 0.0025 | 0.0198 | 0.0031 | 0.0027 | 0.002 | 0.0234 | 0.0018 | 0.0022 |
Bud Periphery | 0.0017 | 0.0023 | 0.0113 | 0.0039 | 0.0056 | 0.0015 | 0.0041 | 0.0056 | 0.0058 | 0.001 | 0.0011 | 0.0035 | 0.0032 | 0.0047 | 0.0078 | 0.0113 | 0.0075 | 0.0032 |
Bud Site | 0.0018 | 0.0358 | 0.019 | 0.0031 | 0.0015 | 0.0019 | 0.0125 | 0.0385 | 0.0166 | 0.0005 | 0.0038 | 0.0024 | 0.0054 | 0.0376 | 0.0102 | 0.0287 | 0.0061 | 0.0011 |
Cell Periphery | 0.0008 | 0.0007 | 0.0014 | 0.0041 | 0.0009 | 0.0007 | 0.0027 | 0.001 | 0.0012 | 0.0002 | 0.0005 | 0.0007 | 0.0011 | 0.0023 | 0.0014 | 0.0042 | 0.0012 | 0.0004 |
Cytoplasm | 0.0137 | 0.013 | 0.0344 | 0.0052 | 0.003 | 0.0456 | 0.0132 | 0.0289 | 0.0229 | 0.0095 | 0.0076 | 0.0168 | 0.0109 | 0.0338 | 0.041 | 0.0242 | 0.0082 | 0.044 |
Cytoplasmic Foci | 0.0083 | 0.1016 | 0.0727 | 0.0186 | 0.0047 | 0.0467 | 0.0687 | 0.0563 | 0.0491 | 0.021 | 0.1086 | 0.0478 | 0.0678 | 0.0551 | 0.0584 | 0.023 | 0.0396 | 0.0473 |
Eisosomes | 0.0004 | 0.0007 | 0.0005 | 0.0011 | 0.0001 | 0.0003 | 0.0008 | 0.0006 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0005 | 0.0007 | 0.0004 | 0.0003 | 0.0002 | 0.0002 |
Endoplasmic Reticulum | 0.0265 | 0.0044 | 0.0042 | 0.0008 | 0.0013 | 0.0018 | 0.0063 | 0.0044 | 0.0043 | 0.0014 | 0.003 | 0.004 | 0.0104 | 0.0041 | 0.0119 | 0.0029 | 0.0051 | 0.0053 |
Endosome | 0.0828 | 0.1937 | 0.1258 | 0.0868 | 0.0912 | 0.0969 | 0.1866 | 0.1572 | 0.1461 | 0.1036 | 0.2129 | 0.1219 | 0.1845 | 0.1992 | 0.1807 | 0.1176 | 0.1177 | 0.156 |
Golgi | 0.0157 | 0.0629 | 0.0676 | 0.051 | 0.0302 | 0.0587 | 0.1004 | 0.0675 | 0.0854 | 0.0369 | 0.1478 | 0.0814 | 0.0922 | 0.1047 | 0.075 | 0.0526 | 0.0493 | 0.0868 |
Lipid Particles | 0.2099 | 0.0072 | 0.0158 | 0.0135 | 0.0009 | 0.0139 | 0.0568 | 0.0095 | 0.0189 | 0.0036 | 0.0555 | 0.0065 | 0.0678 | 0.0145 | 0.0251 | 0.0164 | 0.0085 | 0.0158 |
Mitochondria | 0.1235 | 0.3737 | 0.4623 | 0.6974 | 0.8064 | 0.618 | 0.372 | 0.5344 | 0.537 | 0.6973 | 0.3509 | 0.6267 | 0.4192 | 0.3918 | 0.4595 | 0.5652 | 0.7074 | 0.5753 |
None | 0.3284 | 0.089 | 0.049 | 0.0749 | 0.0004 | 0.0402 | 0.0101 | 0.0026 | 0.0127 | 0.0461 | 0.0011 | 0.0014 | 0.0062 | 0.0161 | 0.0082 | 0.0007 | 0.0011 | 0.0053 |
Nuclear Periphery | 0.0929 | 0.0015 | 0.0018 | 0.0005 | 0.0005 | 0.0006 | 0.0187 | 0.0022 | 0.0056 | 0.0015 | 0.001 | 0.003 | 0.0176 | 0.0045 | 0.0163 | 0.0014 | 0.0032 | 0.0014 |
Nucleolus | 0.002 | 0.0003 | 0.0011 | 0.0011 | 0.0002 | 0.0015 | 0.0017 | 0.0013 | 0.0023 | 0.0002 | 0.0138 | 0.0008 | 0.0035 | 0.0046 | 0.0012 | 0.0005 | 0.0004 | 0.0004 |
Nucleus | 0.0089 | 0.0005 | 0.0014 | 0.0008 | 0.0004 | 0.0019 | 0.0056 | 0.0009 | 0.0017 | 0.0013 | 0.0027 | 0.0011 | 0.0104 | 0.0019 | 0.0081 | 0.0022 | 0.0016 | 0.0011 |
Peroxisomes | 0.0194 | 0.0094 | 0.0704 | 0.0094 | 0.0017 | 0.024 | 0.0341 | 0.0303 | 0.0328 | 0.0204 | 0.0257 | 0.0145 | 0.0376 | 0.0186 | 0.0203 | 0.0527 | 0.007 | 0.0162 |
Punctate Nuclear | 0.0014 | 0.0011 | 0.0016 | 0.0002 | 0 | 0.0007 | 0.0088 | 0.0003 | 0.0005 | 0.0001 | 0.0016 | 0.0002 | 0.0107 | 0.0003 | 0.0066 | 0.0002 | 0.0058 | 0.0006 |
Vacuole | 0.024 | 0.0079 | 0.0284 | 0.0089 | 0.0292 | 0.0139 | 0.0244 | 0.0228 | 0.0243 | 0.0408 | 0.0194 | 0.0169 | 0.0188 | 0.0592 | 0.0232 | 0.0292 | 0.0111 | 0.0172 |
Vacuole Periphery | 0.0303 | 0.0048 | 0.0097 | 0.0102 | 0.0188 | 0.005 | 0.0148 | 0.0094 | 0.0097 | 0.0125 | 0.0122 | 0.0185 | 0.0117 | 0.0225 | 0.0129 | 0.0179 | 0.0094 | 0.0133 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 14.9584 | 13.3719 | 18.6777 | 21.1859 | 13.6901 | 18.4678 | 16.7016 | 15.0299 | 19.0534 | 18.3379 |
Translational Efficiency | 1.2947 | 1.4683 | 1.1691 | 1.0062 | 1.3886 | 1.19 | 1.2424 | 1.4811 | 1.1363 | 1.0867 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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