Standard name
Human Ortholog
Description Component of glycosylphosphatidylinositol-mannosyltransferase I; essential component; required for the autocatalytic post-translational processing of the protease B precursor Prb1p; localizes to ER in lumenal orientation; homolog of mammalian PIG-X
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0 0 0 0 0 0 – 0 0 – 0 0 0 – 0 0.13 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 – 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 – 0.05 0.05 0 – 0.06 0.16 0 0 0 0 0 0
Cytoplasm 0.1 0.28 0.19 0.33 0.25 0.25 0.24 0.31 – 0.37 0.36 – 0.29 0.29 0.41 – 0.72 0.6 0 0.07 0 0 0.05 0.05
Endoplasmic Reticulum 0.87 0.68 0.62 0.37 0.23 0.37 0.31 0.15 – 0.08 0.16 – 0.64 0.66 0.36 – 0 0 0.88 0.76 0.85 0.8 0.76 0.78
Endosome 0 0 0 0.05 0.08 0 0 0 – 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.06 0.05 0.14 0.27 0.23 0.37 0.39 0.42 – 0.53 0.54 – 0 0 0 – 0.12 0 0.06 0.06 0 0.07 0.07 0.05
Nucleus 0 0 0 0 0 0 0 0 – 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0.13 0.14 0.34 0.18 0.19 0.24 – 0.16 0.09 – 0.1 0.1 0.27 – 0.08 0.07 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 6 8 5 4 4 5 5 – 4 3 – 5 8 4 – 5 7 1 0 9 6 22 19
Bud 0 0 4 5 3 1 5 9 – 20 17 – 2 2 0 – 1 2 0 0 2 2 1 3
Bud Neck 0 0 0 1 0 0 0 0 – 0 0 – 0 2 0 – 0 0 0 1 3 3 7 6
Bud Site 0 0 0 0 0 0 1 0 – 0 0 – 0 0 0 – 0 0 – – – – – –
Cell Periphery 1 3 9 1 14 12 8 13 – 17 14 – 31 31 18 – 9 9 0 0 3 3 21 23
Cytoplasm 7 37 74 87 130 97 140 208 – 224 194 – 193 193 207 – 109 33 1 11 13 19 36 48
Endoplasmic Reticulum 60 90 241 97 121 145 183 97 – 48 85 – 425 444 178 – 6 1 90 123 404 412 589 770
Endosome 1 0 7 13 43 5 8 21 – 11 3 – 0 0 3 – 2 1 0 2 3 5 6 6
Golgi 0 0 0 0 0 0 0 0 – 0 0 – 0 0 0 – 0 0 0 1 1 1 1 3
Mitochondria 4 7 53 70 119 146 231 278 – 318 290 – 0 0 2 – 18 2 6 10 20 38 53 50
Nucleus 0 1 1 0 2 4 6 4 – 5 2 – 7 7 3 – 1 0 0 0 0 1 0 0
Nuclear Periphery 1 2 6 0 7 2 14 9 – 7 3 – 1 1 0 – 0 0 0 0 3 1 0 1
Nucleolus 0 0 0 0 0 0 1 1 – 1 1 – 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 1 0 0 1 0 – 0 0 – 0 0 0 – 0 0 0 0 0 1 0 1
SpindlePole 0 0 2 0 0 0 3 0 – 3 0 – 0 0 0 – 2 0 0 0 0 0 0 1
Vac/Vac Membrane 2 7 51 37 178 72 114 163 – 97 48 – 65 67 133 – 12 4 2 8 5 13 32 43
Unique Cell Count 69 133 390 263 516 394 590 667 604 533 661 677 500 151 55 103 161 477 515 780 990
Labelled Cell Count 78 153 456 317 621 488 720 808 755 660 729 755 548 165 59 103 161 477 515 780 990


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 6.3 5.6 5.6 6.0 5.1 4.9 5.2 – 4.7 4.7 – 7.9 7.9 8.3 – 5.8 7.8 7.7 8.1 8.3
Std Deviation (1e-4) 0.9 0.8 0.7 0.8 1.1 0.9 0.8 0.8 – 1.0 1.0 – 0.8 0.8 6.0 – 1.1 1.3 1.1 1.3 1.3
Intensity Change (Log2) – – – -0.01 0.09 -0.13 -0.19 -0.11 – -0.26 -0.25 – 0.49 0.49 0.56 – 0.04 0.47 0.46 0.53 0.56


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.1 -1.7 -1.2 -1.6 0 – 0 0 – 0 -1.1 -1.6 – 0 0
Bud 0 0 0 0 0 – 0 0 – 0 0 0 – 0 0
Bud Neck 0 0 0 0 0 – 0 0 – 0 0 0 – 0 0
Bud Site 0 0 0 0 0 – 0 0 – 0 0 0 – 0 0
Cell Periphery 0 0.4 0.6 -1.1 -0.4 – 0 0 – 1.9 1.9 1.1 – 2.1 0
Cytoplasm 4.1 2.2 1.9 1.8 4.3 – 6.1 5.8 – 3.7 3.5 7.1 – 11.7 6.7
Endoplasmic Reticulum -6.2 -11.7 -7.0 -9.5 -15.9 – 0 0 – 0.8 1.2 -7.8 – -12.1 -8.4
Endosome 2.3 4.3 -0.6 -0.5 1.3 – 0 0 – 0 0 0 – 0 0
Golgi 0 0 0 0 0 – 0 0 – 0 0 0 – 0 0
Mitochondria 4.2 3.6 7.5 8.6 9.5 – 0 0 – -9.7 -9.8 -8.1 – -0.5 -2.1
Nucleus 0 0 0 0 0 – 0 0 – 0 0 0 – 0 0
Nuclear Periphery 0 -0.2 0 0.9 -0.3 – 0 0 – 0 0 0 – 0 0
Nucleolus 0 0 0 0 0 – 0 0 – 0 0 0 – 0 0
Peroxisomes 0 0 0 0 0 – 0 0 – 0 0 0 – 0 0
SpindlePole 0 0 0 0 0 – 0 0 – 0 0 0 – 0 0
Vacuole 0.4 7.3 2.0 2.6 4.4 – 1.3 -2.0 – -1.6 -1.6 4.9 – -1.7 -1.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.7515 2.8596 2.682 1.9002 2.304 2.6001 1.9737 2.0229 1.943 1.7724 1.3168 1.9599 1.1613 2.0044 1.3259 1.6517 0.6559 1.4083
Actin 0.0232 0.0057 0.0224 0.0008 0.0016 0.003 0.0239 0.0004 0.0045 0.0023 0.0002 0.0016 0.0469 0.0018 0.001 0.0004 0.0051 0.0006
Bud 0.0002 0.0002 0.0005 0 0.0001 0.0002 0.0001 0 0.0004 0.0002 0 0.0001 0.0003 0 0.0001 0 0.0004 0
Bud Neck 0.0006 0.0004 0.0021 0.0002 0.0005 0.0011 0.0009 0.0001 0.0044 0.0127 0.0008 0.0005 0.0006 0.0005 0.0011 0.0004 0.0025 0.0007
Bud Periphery 0.0003 0.0003 0.0003 0 0.0001 0.0004 0.0002 0 0.0006 0.0006 0.0001 0.0002 0.0005 0 0.0002 0 0.001 0.0004
Bud Site 0.0003 0.0026 0.003 0.0002 0.0002 0.0001 0.0015 0.0004 0.0134 0.0008 0 0.0001 0.0003 0.0012 0.0003 0 0.0017 0
Cell Periphery 0.0013 0.0024 0.0018 0.0007 0.0004 0.0005 0.0009 0.0007 0.0011 0.0023 0.0004 0.0007 0.001 0.0033 0.0046 0.0021 0.001 0.0576
Cytoplasm 0.1293 0.2716 0.2264 0.1368 0.2674 0.2162 0.065 0.0691 0.1285 0.2745 0.1867 0.142 0.0253 0.2897 0.2054 0.0451 0.222 0.2052
Cytoplasmic Foci 0.0132 0.0111 0.0081 0.0033 0.0055 0.0078 0.0316 0.0014 0.0178 0.0126 0.0021 0.007 0.0061 0.012 0.0028 0.0007 0.0162 0.0043
Eisosomes 0.0003 0.0003 0.0004 0.0002 0 0 0.0004 0.0002 0.0002 0.0002 0 0.0001 0.0007 0.0001 0.0001 0 0.0002 0.0002
Endoplasmic Reticulum 0.6821 0.5633 0.6076 0.7346 0.5195 0.6645 0.6818 0.8245 0.6683 0.5956 0.7385 0.7318 0.6353 0.6132 0.7117 0.9291 0.5621 0.5925
Endosome 0.0155 0.0264 0.017 0.0086 0.025 0.0174 0.0489 0.0097 0.0186 0.0168 0.0155 0.0283 0.0779 0.0227 0.0058 0.0019 0.0271 0.0043
Golgi 0.0039 0.0022 0.0029 0.0005 0.001 0.0008 0.0178 0.0004 0.0063 0.004 0.0004 0.0075 0.1541 0.0019 0.0013 0.0002 0.0107 0.0003
Lipid Particles 0.0084 0.0139 0.0117 0.0027 0.0061 0.0022 0.0353 0.003 0.0143 0.0028 0.001 0.0112 0.0051 0.0218 0.0074 0.0014 0.025 0.0026
Mitochondria 0.0014 0.0011 0.0017 0.0009 0.0015 0.0006 0.0039 0.0003 0.002 0.005 0.0019 0.0045 0.0176 0.0001 0.0002 0.0001 0.0591 0.0001
None 0.0902 0.0648 0.0501 0.0555 0.0811 0.0596 0.0431 0.0595 0.0725 0.0501 0.0048 0.0411 0.002 0.0108 0.0431 0.0049 0.0408 0.1229
Nuclear Periphery 0.0145 0.0186 0.018 0.0169 0.0546 0.0142 0.0264 0.023 0.0209 0.0099 0.0343 0.0117 0.0181 0.0122 0.0087 0.0096 0.0103 0.0038
Nucleolus 0.0005 0.0002 0.0008 0.0036 0.0003 0.0001 0.0005 0 0.0016 0.0001 0 0.0002 0 0 0 0 0.0003 0.0001
Nucleus 0.0021 0.0038 0.0117 0.015 0.0203 0.0054 0.0024 0.0018 0.0025 0.0022 0.0036 0.0026 0.001 0.0013 0.0009 0.0011 0.0026 0.0012
Peroxisomes 0.0031 0.0002 0.003 0.0003 0.0002 0.0001 0.0051 0 0.0121 0.0004 0 0.0004 0.0002 0.0001 0 0 0.001 0
Punctate Nuclear 0.0052 0.0021 0.0042 0.016 0.0057 0.0013 0.0026 0.0003 0.0029 0.0004 0.0003 0.0007 0.0003 0.0012 0.0002 0.0002 0.0012 0.0005
Vacuole 0.0033 0.008 0.0057 0.0024 0.0069 0.0038 0.0059 0.0043 0.0059 0.0053 0.0081 0.0059 0.0042 0.0056 0.0047 0.0026 0.0079 0.0025
Vacuole Periphery 0.0009 0.0008 0.0007 0.0006 0.002 0.0005 0.0017 0.0008 0.0012 0.0011 0.0015 0.0019 0.0025 0.0003 0.0003 0.0002 0.0019 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.8454 12.6155 12.8717 26.9817 19.4408 10.5784 21.1264 23.137 25.1377 27.5259
Translational Efficiency 0.7186 1.0233 0.8549 0.6205 0.6578 1.3472 0.7515 0.6352 0.7039 0.5477

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Component of glycosylphosphatidylinositol-mannosyltransferase I; essential component; required for the autocatalytic post-translational processing of the protease B precursor Prb1p; localizes to ER in lumenal orientation; homolog of mammalian PIG-X
Localization
Cell Percentages ER (97%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Pbn1

Pbn1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pbn1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available