Standard name
Human Ortholog
Description S-phase checkpoint protein required for DNA replication; couples DNA helicase and polymerase; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; defines a novel S-phase checkpoint with Hog1p that coordinates DNA replication and transcription upon osmostress; protects uncapped telomeres; Dia2p-dependent degradation mediates checkpoint recovery; mammalian claspin homolog
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0.06 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.05 0 0 0 0 – 0 0 0.13 0.18 0.17 0.09 0.08 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.09 0.21 0.42 0.16 0.54 0.51 0.46 0.71 – 0.71 0.74 0.08 0 0.19 0 0 0.05 0 0 0 0 0 0
Nucleus 0.94 0.9 0.9 0.83 0.86 0.76 0.78 0.81 0.75 – 0.71 0.66 0.84 0.79 0.72 0.83 0.83 0.82 0.83 0.84 0.81 0.82 0.66 0.43
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.06
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.14
Vac/Vac Membrane 0 0.05 0 0 0 0 0 0 0 – 0 0 0 0 0 0.07 0.08 0.05 0.05 0.09 0.1 0.1 0.18 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 1
Bud 0 0 0 0 0 3 3 2 4 – 8 4 0 1 1 0 0 0 4 6 4 1 5 5
Bud Neck 0 0 1 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 1 0
Bud Site 0 0 2 1 4 4 3 17 6 – 15 9 0 0 2 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 16 10 13 17 10 15 19 12 – 10 5 29 56 55 24 15 24 0 4 2 3 5 3
Endoplasmic Reticulum 3 0 1 0 0 0 0 0 0 – 0 0 0 0 0 6 2 7 0 0 0 0 2 0
Endosome 0 1 1 0 2 0 0 0 0 – 0 0 1 2 0 8 5 0 1 3 1 1 2 22
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 1 2 3 1 0 2
Mitochondria 1 36 56 100 58 133 201 218 200 – 184 213 18 6 59 11 5 11 1 4 1 1 3 10
Nucleus 97 371 245 199 309 186 307 381 211 – 182 190 195 246 228 225 148 169 106 359 231 171 164 133
Nuclear Periphery 0 0 0 1 4 2 9 7 1 – 3 2 1 2 1 0 1 0 0 0 0 0 0 0
Nucleolus 3 5 1 3 8 10 8 18 8 – 6 12 11 9 14 4 4 3 2 3 6 0 3 17
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 2 3 3 0 3 4 1 – 1 1 1 2 0 2 5 1 2 2 5 2 11 45
Vac/Vac Membrane 0 19 3 2 11 4 4 3 4 – 4 6 4 5 8 19 14 11 6 36 28 20 44 58
Unique Cell Count 103 414 271 240 359 246 392 471 283 258 286 231 310 315 270 179 205 128 430 287 208 249 313
Labelled Cell Count 108 448 322 322 417 352 553 669 447 413 442 260 329 368 299 200 227 128 430 287 208 249 313


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 5.1 4.3 4.2 4.5 3.9 3.9 3.8 3.3 – 3.2 3.0 5.3 5.3 4.5 6.7 7.2 7.2 5.1 5.1 5.6
Std Deviation (1e-4) 0.7 1.0 0.8 1.0 1.3 0.9 1.3 1.0 0.7 – 0.6 0.7 1.3 1.3 1.1 1.5 1.7 1.9 1.0 1.5 1.5
Intensity Change (Log2) – – – -0.03 0.09 -0.14 -0.11 -0.18 -0.37 – -0.41 -0.5 0.32 0.32 0.09 0.64 0.75 0.75 0.27 0.27 0.39


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.9 0.6 0.2 0.1 0.2 0.3 – 0.1 -1.4 3.7 5.4 5.3 2.5 2.1 3.4
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 -4.1 -7.3 0 -5.9 -5.4 -4.8
Nucleus -2.5 -1.7 -4.5 -4.1 -3.4 -4.9 – -5.8 -6.8 -2.0 -3.7 -5.5 -2.4 -2.4 -2.6
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 3.2 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 1.6 0 0 0 0 – 0 0 0 0 1.3 3.5 3.7 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.0547 2.9082 2.8522 2.9393 2.1249 2.645 5.1252 3.6242 3.7745 3.6283 3.3681 3.4137 3.9913 4.1686 4.2223 4.5004 3.8035 4.0604
Actin 0.1428 0 0.0208 0.0409 0.0053 0.0004 0.0601 0 0.0096 0.0259 0.0155 0.002 0.0272 0 0.0005 0.0001 0 0.0003
Bud 0.0015 0 0.0002 0.0004 0.0007 0.0012 0.0013 0.0001 0.0005 0.0013 0.0129 0.0006 0.0001 0 0.0001 0 0 0.0002
Bud Neck 0.0161 0.0002 0.0005 0.0005 0.001 0.0015 0.0156 0.0003 0.0051 0.0155 0.0167 0.0022 0.0006 0.0003 0.0002 0.0001 0.0007 0.001
Bud Periphery 0.0032 0 0.0003 0.0003 0.0013 0.0005 0.0033 0.0001 0.0007 0.0027 0.0388 0.0009 0.0004 0 0.0002 0 0 0.0007
Bud Site 0.0091 0.0002 0.0013 0.0021 0.0024 0.0004 0.0228 0.0002 0.008 0.0102 0.0272 0.0006 0.0004 0.0004 0.0004 0 0.0001 0.0002
Cell Periphery 0.0019 0 0.0001 0 0.0004 0.0001 0.0008 0 0.0004 0.0007 0.0011 0.0001 0.0002 0 0.0001 0 0 0
Cytoplasm 0.0264 0.0011 0.0026 0.004 0.0026 0.0027 0.0329 0.001 0.0018 0.0032 0.0015 0.0035 0.0215 0.0048 0.0004 0.0027 0.0006 0.0093
Cytoplasmic Foci 0.0282 0 0.0023 0.0206 0.0124 0.0077 0.0122 0.0001 0.0068 0.0051 0.0017 0.0009 0.0039 0.0001 0 0.0001 0 0.0004
Eisosomes 0.0031 0 0.0005 0.0002 0.0001 0 0.0007 0 0.0001 0.0003 0.0001 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0155 0.0001 0.0045 0.0002 0.0007 0.0005 0.0099 0.0001 0.0014 0.0014 0.0014 0.0038 0.0032 0.0003 0.0003 0.0001 0.0001 0.0003
Endosome 0.0494 0.0001 0.0117 0.0035 0.0307 0.0034 0.0222 0.0001 0.0109 0.0384 0.0057 0.0084 0.0111 0.0007 0.0002 0.0001 0.0001 0.0005
Golgi 0.0237 0 0.0092 0.0059 0.0214 0.0007 0.0105 0 0.0064 0.0068 0.0032 0.002 0.0024 0 0 0 0 0
Lipid Particles 0.0247 0 0.0019 0.01 0.016 0.0023 0.0049 0 0.0063 0.0058 0.0011 0.0004 0.0121 0 0 0 0 0.0004
Mitochondria 0.0121 0.0001 0.0035 0.0015 0.0348 0.0011 0.0138 0.0002 0.0047 0.0237 0.0048 0.0055 0.0067 0.0001 0.0015 0.0001 0.0002 0.0021
None 0.0342 0.0001 0.0011 0.0065 0.0039 0.0031 0.0172 0.0001 0.0004 0.0005 0.0003 0.0009 0.008 0.0094 0.0002 0.0281 0.0001 0.0055
Nuclear Periphery 0.0394 0.0028 0.0081 0.0009 0.0047 0.0058 0.0304 0.0039 0.013 0.0043 0.0033 0.0132 0.0265 0.0055 0.005 0.0023 0.0022 0.0019
Nucleolus 0.0134 0.0102 0.0074 0.0066 0.019 0.0206 0.0129 0.0103 0.0115 0.0043 0.0106 0.0184 0.0142 0.0109 0.0087 0.0054 0.0186 0.0167
Nucleus 0.4977 0.9815 0.9051 0.8582 0.7722 0.9392 0.6896 0.9638 0.8879 0.7925 0.84 0.9296 0.8283 0.9491 0.9805 0.9264 0.9377 0.9565
Peroxisomes 0.0225 0 0.0006 0.0195 0.0095 0.0006 0.0042 0 0.0015 0.0178 0.0007 0.0004 0.0021 0 0 0 0 0.0002
Punctate Nuclear 0.0221 0.0029 0.0161 0.0181 0.05 0.0072 0.0195 0.018 0.0195 0.0028 0.0013 0.0036 0.0142 0.0137 0.0007 0.0341 0.0395 0.0032
Vacuole 0.0091 0.0004 0.0014 0.0002 0.0077 0.0009 0.0076 0.0014 0.0026 0.0296 0.0094 0.0016 0.0113 0.0038 0.0005 0.0001 0.0001 0.0006
Vacuole Periphery 0.0039 0.0001 0.0007 0.0002 0.0031 0.0003 0.0076 0.0003 0.0008 0.0072 0.0025 0.0011 0.0054 0.0005 0.0004 0.0001 0 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.99 8.3921 11.7874 16.5219 27.3864 30.4935 10.9097 7.9769 12.9721 27.8428
Translational Efficiency 1.981 1.451 0.7761 1.1595 1.2746 1.537 0.9082 0.9811 1.02 1.0847

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description S-phase checkpoint protein required for DNA replication; couples DNA helicase and polymerase; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; defines a novel S-phase checkpoint with Hog1p that coordinates DNA replication and transcription upon osmostress; protects uncapped telomeres; Dia2p-dependent degradation mediates checkpoint recovery; mammalian claspin homolog
Localization
Cell Percentages nucleus (86%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Mrc1

Mrc1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrc1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available