Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.09 0.11 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.06 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0.1 0.26 0.09 0.31 0 0.06 0 0 0 0 0.06 0.1 0.16 0.28 0.53 0.51 0.26 0 0.09 0.05 0.06 0.07 0.07
Mitochondria 0.9 0.95 0.87 0.9 0.74 0.85 0.73 0.81 0.88 0.86 0.87 0.87 0.91 0.87 0.79 0.06 0.07 0.08 0.81 0.83 0.81 0.76 0.75 0.75
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.09 0 0 0 0.05 0.08 0.11 0.07 0.11 0.07 0.09 0.08 0.05 0 0 0 0 0.08 0 0 0 0 0 0
Nucleolus 0.13 0 0 0 0.08 0.11 0.11 0.09 0.09 0.12 0.12 0.07 0 0 0 0 0 0.1 0 0 0 0 0 0
Peroxisomes 0 0 0.06 0.07 0 0 0 0 0 0 0 0 0.06 0.05 0.07 0.11 0.11 0.19 0.05 0 0.09 0.07 0.08 0.07
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0.08 0 0.05 0.09 0.07 0.07 0.07 0 0 0 0 0 0.08 0.07 0.23 0.2 0.16 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 3 6 3 1 0 1 4 1 1 3 0 8 7 20 9 8 17 5 8 2 25 31 32
Bud 1 0 0 4 0 2 6 6 3 4 3 3 4 3 7 1 5 7 1 0 0 0 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2
Bud Site 1 0 0 0 0 2 0 2 0 2 1 1 0 0 0 0 0 1 – – – – – –
Cell Periphery 2 12 2 2 0 0 3 3 0 2 0 0 20 13 30 27 21 23 0 0 1 1 1 1
Cytoplasm 1 0 1 2 4 12 11 12 8 14 11 6 4 16 20 6 3 5 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 1 1 2 2 4 1 2 2 3 10 11 12 2 5 4 0 0 0 1 2 3
Endosome 1 1 4 4 3 3 8 13 2 6 2 3 7 24 18 2 5 2 1 4 2 13 13 22
Golgi 2 37 35 12 40 0 17 14 3 7 5 10 73 146 275 118 123 53 9 35 7 44 56 59
Mitochondria 179 347 116 117 97 180 216 364 223 270 174 142 649 801 790 13 17 16 165 313 120 531 632 654
Nucleus 1 0 0 0 0 1 0 0 0 2 1 1 0 0 0 1 1 1 0 0 0 0 2 0
Nuclear Periphery 17 14 3 2 7 16 31 33 27 21 17 13 37 37 21 4 6 17 1 2 1 8 11 9
Nucleolus 26 10 5 1 10 23 32 41 24 36 24 12 27 29 14 5 8 20 2 2 0 3 3 7
Peroxisomes 8 2 8 9 1 1 8 10 2 0 1 2 41 45 66 24 26 39 10 4 13 47 63 59
SpindlePole 1 0 2 3 2 2 6 5 3 0 2 1 2 3 1 7 3 2 3 0 0 2 3 5
Vac/Vac Membrane 13 30 4 7 12 14 21 30 5 13 5 6 27 70 69 52 49 34 1 5 0 12 8 11
Unique Cell Count 198 367 133 130 131 212 294 452 253 313 199 164 717 918 994 222 243 207 204 379 149 698 843 877
Labelled Cell Count 253 456 186 167 178 258 362 541 302 380 251 203 909 1205 1343 271 280 241 204 379 149 698 843 877


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.4 14.0 12.8 12.9 15.0 9.8 13.1 12.9 12.0 12.2 11.3 11.7 14.8 14.6 15.1 16.9 15.2 13.9 12.5 12.8 13.2
Std Deviation (1e-4) 3.0 2.3 2.3 4.0 3.4 3.0 3.5 3.3 3.1 3.4 3.7 3.3 3.4 3.3 3.2 8.6 4.2 4.3 3.2 3.3 3.1
Intensity Change (Log2) – – – 0.0 0.23 -0.39 0.03 0.01 -0.1 -0.07 -0.19 -0.14 0.21 0.19 0.23 0.39 0.25 0.11 -0.05 -0.0 0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 -0.2 0 1.3
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 3.5 2.8 3.3
Cytoplasm 0 0 2.3 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi -3.6 0.8 -7.9 -6.0 -8.5 -7.9 -8.0 -6.5 -4.8 -5.1 -3.0 0.3 4.9 4.6 -0.1
Mitochondria 0.7 -2.7 -0.6 -3.2 -1.8 0.3 -0.3 0.1 -0.2 1.2 0 -2.1 -15.4 -15.6 -14.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 2.1 2.9 2.1 2.9 1.9 2.4 2.2 1.5 1.0 0 0 0 2.3
Nucleolus 0 1.4 2.3 2.4 2.0 2.0 2.6 2.6 1.3 0 0 0 0 0 2.0
Peroxisomes 0.3 0 0 0 0 0 0 0 0 -0.1 -0.5 0.3 1.5 1.5 3.3
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.0 2.1 1.5 1.7 1.6 0 0.6 0 0 0 1.9 1.7 5.1 4.6 3.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.6975 9.6957 9.1021 8.4719 7.949 9.1576 15.1613 14.1767 14.4847 15.5493 14.0474 14.3618 15.5366 15.9977 15.8716 15.2769 12.7166 15.4572
Actin 0.0301 0.0038 0.0076 0.0029 0.0576 0.0118 0.005 0.0017 0.0035 0.0015 0.0003 0.0007 0.0353 0.0022 0.0069 0.0003 0.0317 0.0002
Bud 0.0005 0.0004 0.0004 0.0001 0.0003 0.0002 0.0001 0.0003 0.0001 0.0001 0 0 0.0002 0.0001 0.0006 0 0.0002 0
Bud Neck 0.0011 0.0001 0.0002 0 0.0013 0.0004 0.0002 0.0001 0.0002 0 0 0.0001 0.0003 0.0001 0.0002 0 0.0002 0
Bud Periphery 0.001 0.0006 0.0007 0.0001 0.0007 0.0004 0.0001 0.0006 0.0003 0.0003 0 0.0001 0.0004 0.0003 0.001 0 0.0004 0.0001
Bud Site 0.0033 0.0009 0.0012 0.0001 0.0008 0.0002 0.0001 0.0011 0.0008 0.0001 0 0 0.0004 0.0002 0.0013 0 0.0005 0
Cell Periphery 0.0029 0.0003 0.0002 0.0001 0.0003 0.0001 0.0002 0.0007 0.0006 0.0001 0 0.0002 0.0002 0.0001 0.0002 0 0.0001 0.0001
Cytoplasm 0.0079 0.0001 0.0011 0.0001 0.0003 0.0008 0 0.0001 0.0001 0.0003 0 0 0.0009 0 0.0005 0 0.0055 0
Cytoplasmic Foci 0.0298 0.0023 0.003 0.0094 0.0051 0.0021 0.0006 0.0023 0.0006 0.0031 0.0003 0.0001 0.0067 0.0005 0.0026 0.0001 0.0207 0
Eisosomes 0.0028 0.0002 0.0002 0.0001 0.0026 0.0001 0.0005 0.0002 0.0003 0.0001 0.0002 0.0002 0.0008 0.0001 0.0005 0 0.0006 0.0001
Endoplasmic Reticulum 0.005 0.0001 0.0015 0.0002 0.0012 0.0018 0.0003 0.0002 0.0008 0.0001 0 0.0001 0.0018 0.0001 0.0028 0 0.0039 0.0001
Endosome 0.0373 0.0095 0.0138 0.0044 0.0101 0.0091 0.0038 0.0123 0.0073 0.0033 0.002 0.0018 0.022 0.0049 0.0122 0.0021 0.0186 0.0006
Golgi 0.0118 0.0248 0.0337 0.0041 0.0116 0.0063 0.0108 0.0201 0.02 0.0204 0.0132 0.0133 0.0131 0.0108 0.0103 0.0143 0.0137 0.0016
Lipid Particles 0.0181 0.0065 0.0074 0.0169 0.0114 0.0098 0.0041 0.0094 0.0051 0.0013 0 0.0006 0.0181 0.0021 0.0026 0.0002 0.0234 0.0039
Mitochondria 0.7798 0.9284 0.9 0.9418 0.8736 0.9396 0.9618 0.9232 0.9379 0.9619 0.9832 0.9755 0.8607 0.9629 0.9401 0.9817 0.81 0.9842
None 0.005 0 0.0007 0.0004 0.0013 0.0002 0.0002 0 0 0 0 0 0.0045 0 0.0002 0 0.0189 0
Nuclear Periphery 0.0186 0.0001 0.0073 0.0003 0.0053 0.0015 0.0016 0.0001 0.0005 0 0 0.0001 0.0069 0.0004 0.0017 0 0.0069 0.0009
Nucleolus 0.0013 0.0004 0.0003 0.0001 0.0003 0.0002 0.0016 0.0033 0.0003 0 0 0 0.0004 0.0001 0.0001 0 0.0005 0.0001
Nucleus 0.0103 0.0001 0.001 0.0001 0.0005 0.0003 0.0003 0.0004 0.0001 0 0 0 0.0006 0.0001 0.0004 0 0.001 0.0001
Peroxisomes 0.008 0.0038 0.0035 0.0156 0.0057 0.0016 0.0015 0.0026 0.0009 0.006 0.0001 0.0002 0.0065 0.0014 0.0049 0.0003 0.0226 0.0006
Punctate Nuclear 0.0119 0 0.0005 0.0013 0.0028 0.0014 0.0001 0 0 0 0 0 0.0044 0 0.0001 0 0.0181 0
Vacuole 0.0049 0.0062 0.0031 0.0004 0.0011 0.0011 0.002 0.0078 0.0071 0.0004 0 0.0022 0.0077 0.0019 0.0024 0.0001 0.0008 0.0008
Vacuole Periphery 0.0087 0.0113 0.0126 0.0015 0.006 0.011 0.0052 0.0133 0.0134 0.001 0.0005 0.0048 0.0083 0.0115 0.0084 0.0009 0.0016 0.0068

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.9919 32.1644 26.5193 23.4359 19.4377 13.9847 23.9394 29.8761 27.6892 24.8678
Translational Efficiency 2.0672 0.9645 1.1688 1.2297 1.7199 3.0407 1.9362 1.8835 2.0241 2.0652

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages mitochondrion (99%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Mrpl32

Mrpl32


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrpl32-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available