ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0.06 0 0 0 0 0.07 0.11 0.13 0.06 0.11 0.1 0 0 0 0.12 0.11 0.08
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0.08 0.07 0.08 0 0 0 0 0.07 0 0.09 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0 0.17 0.06 0.07 0.06 0.23 0.16 0.22 0.21 0 0 0 0.11 0 0.06 0 0 0 0 0.08 0
Nucleus 0.28 0.22 0.17 0.11 0.11 0.1 0.09 0.14 0.12 0.14 0.15 0.17 0.14 0.08 0.08 0.26 0.16 0.19 0.17 0.13 0.13 0.08 0.05 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.81 0.82 0.79 0.85 0.82 0.76 0.79 0.71 0.75 0.72 0.77 0.7 0.84 0.79 0.79 0.74 0.73 0.74 0.73 0.73 0.63 0.52 0.45 0.55
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.05 0.11 0.26 0.35 0.28 0.39 0.43 0.41 0.36 0.41 0.26 0.27 0.29 0.37 0.35 0.06 0.06 0.1 0 0.07 0.13 0.12 0.15 0.13
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0
Bud 0 1 0 0 2 2 4 1 8 7 2 9 0 0 0 0 0 0 6 3 2 4 8 9
Bud Neck 3 1 5 3 11 3 3 3 2 1 2 5 0 8 3 1 0 0 0 0 0 0 3 5
Bud Site 0 3 1 4 9 8 8 1 22 14 9 28 0 0 0 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 1 0 1
Cytoplasm 4 5 10 5 3 9 17 22 9 8 4 6 11 28 38 17 18 15 0 0 2 31 32 25
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 3 1 2 0 0 0 2 5 2
Endosome 5 11 9 8 4 29 30 32 9 21 11 8 11 11 26 6 3 1 6 9 12 9 9 9
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 1 1 5 3 0 1 2
Mitochondria 20 22 5 6 35 22 30 22 115 76 78 104 1 2 1 29 6 10 2 7 0 10 25 9
Nucleus 121 107 48 22 22 38 42 55 58 68 54 85 23 21 25 69 26 29 76 69 40 19 13 11
Nuclear Periphery 2 6 4 0 1 2 2 2 0 12 3 3 0 0 0 0 0 0 1 1 1 1 2 7
Nucleolus 352 403 228 172 167 282 360 277 374 349 275 342 141 199 236 197 115 115 335 379 189 134 138 172
Peroxisomes 0 3 2 0 0 4 3 2 5 4 1 1 1 1 3 0 0 1 0 0 0 0 0 0
SpindlePole 21 56 76 71 57 145 197 162 179 200 94 135 48 93 104 17 10 15 20 36 37 30 46 40
Vac/Vac Membrane 4 7 1 2 0 3 0 2 2 0 1 2 1 3 3 8 3 3 6 6 8 3 8 7
Unique Cell Count 436 491 289 203 204 369 453 391 502 484 355 492 168 253 297 268 158 155 461 523 301 256 307 313
Labelled Cell Count 532 625 389 293 311 547 696 581 783 760 535 728 239 367 439 348 184 192 461 523 301 256 307 313


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.2 9.6 9.9 8.6 6.1 6.8 6.7 6.8 5.6 6.0 5.7 5.7 9.8 9.5 9.2 10.7 13.6 14.3 9.6 10.2 11.0
Std Deviation (1e-4) 2.7 2.3 2.6 2.0 1.6 1.6 1.7 1.9 1.9 1.6 1.4 1.6 2.8 3.1 3.0 3.5 4.3 6.3 2.4 2.5 2.9
Intensity Change (Log2) – – – -0.21 -0.7 -0.54 -0.56 -0.54 -0.83 -0.72 -0.8 -0.81 -0.02 -0.07 -0.11 0.1 0.45 0.52 -0.05 0.04 0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 2.3 0 0 0 0 0 0 0 0 1.1 0 0 0 0
Bud Site 0 0 0 0 0 3.3 2.5 0 3.8 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.6 -1.4 -0.8 0.2 1.3 -1.5 -1.6 -2.0 -2.1 1.5 3.5 4.1 1.6 3.3 2.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.5 -0.8 2.6 2.1 2.7 -1.2 0.9 0 -1.4 1.7 0.8 2.9 -0.6 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 6.2 2.7 3.1 2.6 8.0 6.1 7.6 7.6 0 0 0 4.5 0 2.6
Nucleus -1.8 -1.8 -2.4 -3.0 -0.9 -2.0 -1.0 -0.5 0.2 -0.8 -2.9 -3.0 2.6 0 0.6
Nuclear Periphery 0 0 0 0 0 0 1.0 0 0 0 0 0 0 0 0
Nucleolus 1.6 0.8 -0.8 0.2 -2.4 -1.4 -2.1 -0.4 -2.9 1.3 -0.1 0.2 -1.5 -1.5 -1.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 2.1 0.4 3.5 4.7 4.1 2.7 4.2 0.1 0.3 0.5 2.6 2.3 -6.3 -5.1 -4.1
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 10.1621 11.9156 10.3506 9.693 10.3087 10.3349 10.3852 15.3347 13.0148 11.3454 13.018 13.0162 10.6863 11.0685 10.4345 9.3973 10.3193 10.4072
Actin 0.0199 0 0.0074 0 0.0002 0.0021 0.0005 0 0.0162 0.0734 0.0002 0 0.0158 0.0003 0.0013 0.0085 0 0.001
Bud 0.0002 0 0.0001 0 0.0001 0 0.0002 0.0001 0.0004 0.0003 0.0008 0 0.0002 0.0001 0 0.0001 0 0
Bud Neck 0.0057 0.0003 0.0004 0.0002 0.0014 0.0002 0.0004 0.0001 0.0006 0.0004 0.0012 0.0001 0.0008 0.0002 0.0002 0.0004 0.0002 0.0003
Bud Periphery 0.0004 0 0.0003 0 0.0003 0 0.0003 0.0001 0.0013 0.0004 0.0017 0 0.0002 0.0002 0.0001 0.0002 0 0
Bud Site 0.0066 0.0002 0.001 0.0001 0.0002 0.0001 0.0009 0.0005 0.0019 0.0006 0.0019 0 0.0014 0.0012 0.0002 0.0008 0 0.0002
Cell Periphery 0.0004 0.0001 0.0002 0.0002 0.0001 0 0.0008 0.0001 0.001 0.0001 0.0004 0 0.0002 0.0001 0.0001 0.0006 0.0001 0
Cytoplasm 0.001 0 0.0007 0 0 0 0.1292 0 0.0029 0.0002 0.0035 0 0.0048 0 0.0003 0.0016 0 0
Cytoplasmic Foci 0.0059 0.0001 0.0074 0.0001 0.0013 0.0008 0.0319 0.0001 0.0236 0.0161 0.0053 0.0002 0.012 0.0025 0.0083 0.0175 0 0.0019
Eisosomes 0.0004 0 0.0001 0 0.0001 0 0.0001 0 0.0004 0.0002 0.0001 0 0.0001 0 0 0.0003 0 0
Endoplasmic Reticulum 0.0011 0 0.0009 0 0 0 0.0046 0 0.0064 0.0002 0.002 0 0.0019 0 0.0004 0.0082 0 0
Endosome 0.0046 0 0.0067 0 0.0003 0.0008 0.0059 0 0.0185 0.012 0.1139 0 0.0157 0.0002 0.0034 0.0049 0 0.0004
Golgi 0.0066 0 0.0021 0 0.0003 0.002 0.001 0 0.0148 0.0531 0.006 0 0.0045 0.0003 0.0009 0.0033 0 0.001
Lipid Particles 0.0095 0.0003 0.0048 0.0002 0.0048 0.0023 0.0195 0.0002 0.0146 0.0128 0.0033 0.0013 0.0045 0.0018 0.0114 0.0199 0.0001 0.0027
Mitochondria 0.0151 0 0.0037 0.0001 0.0037 0.0013 0.008 0.0011 0.017 0.0065 0.0036 0.0001 0.0012 0.0009 0.0038 0.0158 0 0.0004
None 0.0017 0 0.0026 0 0.0001 0 0.0034 0 0.0041 0.0002 0.0004 0 0.0007 0 0.0001 0.0029 0 0
Nuclear Periphery 0.007 0 0.0049 0 0.0013 0.0001 0.0054 0 0.0239 0.0001 0.0033 0 0.0061 0 0.001 0.0108 0 0.0001
Nucleolus 0.8159 0.867 0.867 0.9189 0.8454 0.8952 0.6886 0.8253 0.748 0.7062 0.7358 0.9053 0.8296 0.84 0.8715 0.7238 0.8851 0.8919
Nucleus 0.0872 0.1291 0.0833 0.0793 0.0891 0.0829 0.0544 0.1711 0.0727 0.0706 0.0755 0.0875 0.0837 0.148 0.0839 0.15 0.1095 0.0941
Peroxisomes 0.0049 0 0.0026 0 0.0004 0.002 0.0016 0 0.02 0.0457 0.0005 0 0.0017 0.0019 0.0047 0.0114 0 0.001
Punctate Nuclear 0.0048 0.0023 0.002 0.0005 0.0502 0.0099 0.0311 0.0008 0.0021 0.0008 0.0102 0.0052 0.013 0.0019 0.007 0.013 0.0046 0.0044
Vacuole 0.0007 0.0004 0.0011 0.0003 0.0004 0.0001 0.0072 0.0002 0.0051 0.0002 0.0243 0.0001 0.0013 0.0003 0.0009 0.0022 0.0001 0.0002
Vacuole Periphery 0.0005 0 0.0006 0.0001 0.0004 0 0.0051 0.0002 0.0043 0.0002 0.0062 0 0.0006 0.0001 0.0005 0.0038 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 32.862 31.6363 25.9908 28.8423 27.0391 32.8438 29.4171 19.2762 25.3563 34.4046
Translational Efficiency 3.4997 2.6678 2.9045 2.2232 3.3553 2.2284 1.7574 2.0858 1.6693 1.8923

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis
Localization
Cell Percentages nucleolus (79%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-2

YCR016W

YCR016W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ycr016w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available