Standard name | |
---|---|
Human Ortholog | |
Description | Putative Rheb-related GTPase; involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 |
Bud | 1 | 1 | 0 | 0 | 1 | 4 | 5 | 3 | – | 4 | 7 | 6 | 2 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 10 | 10 | 0 | 4 | 4 | 9 | 4 | 5 | – | 10 | 7 | 12 | 5 | 3 | 8 | 5 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 324 | 306 | 231 | 289 | 347 | 350 | 430 | 387 | – | 318 | 262 | 292 | 201 | 269 | 302 | 111 | 48 | 72 | 330 | 312 | 221 | 117 | 201 | 244 |
Endoplasmic Reticulum | 3 | 2 | 2 | 1 | 1 | 1 | 3 | 0 | – | 6 | 1 | 3 | 0 | 1 | 1 | 2 | 0 | 2 | 1 | 0 | 0 | 0 | 7 | 2 |
Endosome | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | – | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 5 | 1 | 0 | 0 | 1 | 1 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 2 |
Mitochondria | 0 | 1 | 6 | 12 | 23 | 80 | 177 | 152 | – | 162 | 137 | 155 | 0 | 0 | 0 | 2 | 1 | 3 | 1 | 0 | 0 | 2 | 2 | 2 |
Nucleus | 4 | 3 | 0 | 0 | 1 | 3 | 2 | 6 | – | 1 | 1 | 3 | 1 | 0 | 2 | 2 | 0 | 4 | 0 | 0 | 1 | 1 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 0 | 3 | 4 | 0 | – | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | 0 | 1 | 0 | 4 | 10 | 13 | – | 17 | 8 | 10 | 1 | 1 | 0 | 1 | 0 | 1 | 9 | 7 | 0 | 13 | 5 | 4 |
Unique Cell Count | 327 | 312 | 235 | 293 | 359 | 387 | 516 | 473 | 430 | 350 | 404 | 202 | 272 | 306 | 115 | 49 | 74 | 357 | 324 | 228 | 143 | 226 | 266 | |
Labelled Cell Count | 343 | 324 | 240 | 307 | 378 | 455 | 635 | 567 | 522 | 425 | 484 | 210 | 276 | 314 | 124 | 51 | 84 | 357 | 324 | 228 | 143 | 226 | 266 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.0 | 8.6 | 6.0 | 6.2 | 5.8 | 5.3 | 4.8 | 5.1 | – | 4.7 | 4.9 | 5.0 | 8.5 | 8.5 | 8.4 | 7.7 | 7.8 | 7.8 | 8.7 | 9.7 | 10.4 |
Std Deviation (1e-4) | 1.1 | 1.1 | 0.9 | 1.4 | 1.4 | 1.3 | 1.4 | 1.3 | – | 0.9 | 0.9 | 1.1 | 1.2 | 1.2 | 1.1 | 1.0 | 1.5 | 1.2 | 1.8 | 1.6 | 1.6 |
Intensity Change (Log2) | – | – | – | 0.03 | -0.05 | -0.18 | -0.32 | -0.22 | – | -0.36 | -0.29 | -0.26 | 0.51 | 0.49 | 0.47 | 0.36 | 0.37 | 0.37 | 0.53 | 0.69 | 0.78 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.3 | -1.2 | -3.8 | -5.9 | -6.2 | – | -7.9 | -7.6 | -8.2 | 1.2 | 0.6 | 0.4 | -1.0 | -0.2 | -0.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.0 | 2.1 | 6.3 | 0 | 8.9 | – | 0 | 10.1 | 10.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | – | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.6181 | 3.056 | 2.6451 | 2.1614 | 2.782 | 2.6462 | 3.5621 | 4.0211 | 3.7442 | 3.5186 | 4.0361 | 4.1119 | 2.3328 | 2.7173 | 2.4444 | 2.4436 | 2.0388 | 2.3965 |
Actin | 0.011 | 0.0013 | 0.0093 | 0.0239 | 0.0329 | 0.005 | 0.0438 | 0.0016 | 0.0175 | 0.0003 | 0.045 | 0.0058 | 0.0004 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0.0002 |
Bud | 0.0036 | 0.0057 | 0.0008 | 0.0007 | 0.0003 | 0.0083 | 0.0003 | 0.0005 | 0.0009 | 0.0004 | 0.0038 | 0.0103 | 0 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0 |
Bud Neck | 0.0151 | 0.0002 | 0.0007 | 0.0006 | 0.0024 | 0.0021 | 0.0008 | 0.0003 | 0.0004 | 0.0002 | 0.0009 | 0.0023 | 0.0003 | 0.0001 | 0.0002 | 0.0003 | 0.0005 | 0.0007 |
Bud Periphery | 0.0029 | 0.0016 | 0.0011 | 0.0009 | 0.0004 | 0.0053 | 0.0005 | 0.0008 | 0.0008 | 0.0005 | 0.0035 | 0.0091 | 0 | 0 | 0.0001 | 0.0004 | 0.0001 | 0 |
Bud Site | 0.0022 | 0.0122 | 0.0015 | 0.0008 | 0.0004 | 0.0003 | 0.0006 | 0.0043 | 0.0066 | 0.0002 | 0.004 | 0.0011 | 0.0003 | 0.0003 | 0.0004 | 0.0002 | 0.0001 | 0 |
Cell Periphery | 0.0004 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0007 | 0.0005 | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 |
Cytoplasm | 0.6811 | 0.8119 | 0.7356 | 0.7567 | 0.589 | 0.7754 | 0.7618 | 0.7666 | 0.8059 | 0.8872 | 0.6731 | 0.7956 | 0.8617 | 0.8938 | 0.9005 | 0.8683 | 0.9032 | 0.9039 |
Cytoplasmic Foci | 0.0067 | 0.0053 | 0.009 | 0.0232 | 0.0059 | 0.0135 | 0.0043 | 0.0079 | 0.0063 | 0.01 | 0.0124 | 0.0121 | 0.0043 | 0.0033 | 0.0047 | 0.0111 | 0.0057 | 0.0032 |
Eisosomes | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0003 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0039 | 0.0023 | 0.0031 | 0.0071 | 0.002 | 0.0015 | 0.0048 | 0.0041 | 0.0035 | 0.0026 | 0.0093 | 0.002 | 0.0024 | 0.0011 | 0.0022 | 0.0026 | 0.0029 | 0.0012 |
Endosome | 0.009 | 0.0021 | 0.0045 | 0.0115 | 0.0039 | 0.0013 | 0.0038 | 0.0031 | 0.0028 | 0.0038 | 0.0087 | 0.0027 | 0.0015 | 0.0012 | 0.0022 | 0.0085 | 0.0018 | 0.001 |
Golgi | 0.0015 | 0.0002 | 0.0009 | 0.0055 | 0.003 | 0.0004 | 0.0041 | 0.0006 | 0.001 | 0.0003 | 0.0034 | 0.0005 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0001 |
Lipid Particles | 0.0005 | 0.0001 | 0.0007 | 0.0045 | 0.0021 | 0.0009 | 0.001 | 0.0003 | 0.0003 | 0.0001 | 0.0006 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 |
Mitochondria | 0.0009 | 0.001 | 0.0006 | 0.0012 | 0.0005 | 0.0004 | 0.001 | 0.0003 | 0.0002 | 0.0001 | 0.0029 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 |
None | 0.2525 | 0.1495 | 0.2254 | 0.1478 | 0.3515 | 0.1779 | 0.1673 | 0.2018 | 0.1499 | 0.0898 | 0.2238 | 0.1526 | 0.1239 | 0.0971 | 0.0842 | 0.1028 | 0.0813 | 0.0877 |
Nuclear Periphery | 0.0027 | 0.0008 | 0.0012 | 0.002 | 0.0012 | 0.0005 | 0.0014 | 0.0006 | 0.0007 | 0.0005 | 0.0021 | 0.0004 | 0.0013 | 0.0004 | 0.0006 | 0.0008 | 0.0006 | 0.0002 |
Nucleolus | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0002 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 |
Nucleus | 0.0021 | 0.0022 | 0.0016 | 0.0015 | 0.0008 | 0.0014 | 0.001 | 0.0016 | 0.001 | 0.001 | 0.0016 | 0.0015 | 0.0025 | 0.0014 | 0.0025 | 0.0016 | 0.0017 | 0.0009 |
Peroxisomes | 0.0005 | 0.0001 | 0.0007 | 0.0082 | 0.0006 | 0.004 | 0.001 | 0.0001 | 0.0002 | 0.0001 | 0.0017 | 0.0005 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 |
Punctate Nuclear | 0.0013 | 0.0014 | 0.0012 | 0.0019 | 0.0018 | 0.0009 | 0.0009 | 0.0008 | 0.0006 | 0.0002 | 0.0015 | 0.0013 | 0.0003 | 0.0005 | 0.0005 | 0.0005 | 0.0006 | 0.0002 |
Vacuole | 0.0013 | 0.0012 | 0.0014 | 0.0014 | 0.0007 | 0.0005 | 0.0005 | 0.0035 | 0.0008 | 0.0021 | 0.0012 | 0.0012 | 0.0006 | 0.0004 | 0.0007 | 0.0013 | 0.0006 | 0.0003 |
Vacuole Periphery | 0.0002 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.1358 | 14.0911 | 13.9416 | 12.8241 | 10.2187 | 7.0019 | 13.0602 | 10.6845 | 14.5008 | 13.2223 |
Translational Efficiency | 0.6414 | 0.7845 | 0.8031 | 1.2316 | 1.3283 | 1.4552 | 1.0548 | 1.2647 | 0.823 | 1.0007 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|