Standard name | |
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Human Ortholog | |
Description | Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 1 | – | 0 | 0 | 0 | 2 | 5 | 5 | 2 | 1 | 0 | 1 | 1 | 1 | 0 | 3 | 4 |
Bud | 0 | 4 | 1 | 0 | 0 | 2 | 5 | 6 | – | 6 | 3 | 2 | 1 | 2 | 1 | 0 | 0 | 0 | 2 | 6 | 7 | 10 | 9 | 8 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 2 | 4 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 4 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | – | 0 | 1 | 1 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 24 | 54 | 68 | 45 | 97 | 191 | 289 | 280 | – | 328 | 272 | 334 | 194 | 457 | 563 | 64 | 73 | 48 | 3 | 8 | 4 | 9 | 4 | 5 |
Endoplasmic Reticulum | 3 | 14 | 17 | 0 | 4 | 6 | 12 | 5 | – | 4 | 0 | 0 | 9 | 19 | 6 | 10 | 19 | 14 | 3 | 4 | 9 | 8 | 13 | 22 |
Endosome | 27 | 14 | 18 | 1 | 19 | 5 | 2 | 0 | – | 3 | 0 | 0 | 14 | 19 | 3 | 13 | 13 | 16 | 12 | 12 | 9 | 18 | 18 | 27 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 1 | 1 | 2 | 0 | 0 | 3 | 7 |
Mitochondria | 4 | 36 | 11 | 1 | 6 | 36 | 51 | 22 | – | 51 | 36 | 28 | 0 | 0 | 0 | 12 | 12 | 8 | 3 | 8 | 1 | 10 | 5 | 12 |
Nucleus | 62 | 61 | 92 | 57 | 53 | 53 | 38 | 32 | – | 25 | 12 | 12 | 28 | 8 | 2 | 103 | 122 | 103 | 0 | 1 | 0 | 1 | 1 | 2 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 0 | 2 | 9 | 2 | – | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 3 | 6 | 5 |
Nucleolus | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 2 | 1 | 5 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 3 | 2 | 0 | 6 | 0 | 3 | 0 | 2 | 0 | 2 |
Vac/Vac Membrane | 177 | 257 | 210 | 168 | 244 | 271 | 298 | 189 | – | 182 | 92 | 127 | 152 | 88 | 33 | 155 | 200 | 171 | 265 | 413 | 395 | 426 | 736 | 628 |
Unique Cell Count | 254 | 380 | 363 | 211 | 342 | 442 | 554 | 463 | 497 | 343 | 428 | 350 | 573 | 606 | 297 | 355 | 296 | 298 | 470 | 433 | 498 | 811 | 735 | |
Labelled Cell Count | 300 | 444 | 426 | 272 | 426 | 566 | 704 | 540 | 601 | 418 | 507 | 400 | 604 | 621 | 363 | 443 | 369 | 298 | 470 | 433 | 498 | 811 | 735 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.5 | 7.2 | 6.7 | 6.9 | 6.7 | 5.8 | 5.6 | 5.8 | – | 5.0 | 5.1 | 5.3 | 7.0 | 6.8 | 6.6 | 7.5 | 8.4 | 9.3 | 7.9 | 8.4 | 8.6 |
Std Deviation (1e-4) | 1.8 | 1.7 | 1.4 | 1.4 | 1.7 | 1.8 | 1.7 | 1.5 | – | 1.4 | 1.5 | 1.4 | 1.1 | 1.1 | 1.1 | 1.7 | 2.1 | 2.6 | 1.7 | 1.6 | 1.6 |
Intensity Change (Log2) | – | – | – | 0.03 | 0.0 | -0.2 | -0.26 | -0.22 | – | -0.41 | -0.38 | -0.35 | 0.06 | 0.02 | -0.03 | 0.16 | 0.32 | 0.47 | 0.23 | 0.33 | 0.36 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.8 | 3.0 | 7.4 | 10.2 | 12.1 | – | 13.7 | 16.1 | 16.6 | 10.2 | 18.3 | 23.4 | 0.9 | 0.6 | -0.8 |
Endoplasmic Reticulum | -3.2 | -2.7 | -2.8 | -2.1 | -3.2 | – | -3.6 | -4.1 | -4.5 | -1.5 | -1.1 | -3.7 | -0.8 | 0.4 | 0 |
Endosome | -2.9 | 0.4 | -3.2 | -4.7 | -4.8 | – | -4.1 | -4.2 | -4.7 | -0.6 | -1.3 | -4.6 | -0.4 | -0.9 | 0.3 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | -1.1 | 3.1 | 3.6 | 1.3 | – | 4.0 | 4.0 | 2.3 | -3.3 | 0 | 0 | 0.7 | 0.3 | -0.2 |
Nucleus | 0.4 | -3.2 | -4.9 | -7.8 | -7.4 | – | -8.6 | -8.2 | -9.3 | -6.2 | -11.6 | -12.7 | 2.6 | 2.6 | 2.6 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 5.3 | 3.7 | 1.0 | -1.2 | -4.9 | – | -6.2 | -8.3 | -8.0 | -3.9 | -13.6 | -18.2 | -1.5 | -0.4 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | – | – | – | – | – | – | 0.0021 | 0 | 0.0141 | 0 | 0.0273 | 0.0003 | 0 | 0.0004 | 0.0001 | 0 | 0 | 0 |
Bud | – | – | – | – | – | – | 0.0004 | 0.0043 | 0.001 | 0.0004 | 0.0005 | 0.0004 | 0.0003 | 0.0005 | 0.0007 | 0.0003 | 0.0003 | 0.0005 |
Bud Neck | – | – | – | – | – | – | 0.0017 | 0.002 | 0.0034 | 0.0013 | 0.0022 | 0.0035 | 0.0009 | 0.0022 | 0.0066 | 0.002 | 0.0031 | 0.0021 |
Bud Periphery | – | – | – | – | – | – | 0.0016 | 0.0021 | 0.0023 | 0.0011 | 0.0015 | 0.0014 | 0.0005 | 0.0009 | 0.0016 | 0.0005 | 0.0008 | 0.0012 |
Bud Site | – | – | – | – | – | – | 0.0013 | 0.0115 | 0.0062 | 0.0013 | 0.0016 | 0.0012 | 0.0009 | 0.0023 | 0.0027 | 0.0019 | 0.0006 | 0.0017 |
Cell Periphery | – | – | – | – | – | – | 0.008 | 0.0038 | 0.0038 | 0.0031 | 0.0038 | 0.0039 | 0.0011 | 0.0028 | 0.0023 | 0.003 | 0.0021 | 0.0036 |
Cytoplasm | – | – | – | – | – | – | 0.0272 | 0.0151 | 0.0193 | 0.0131 | 0.0051 | 0.0099 | 0.0531 | 0.0476 | 0.0656 | 0.0089 | 0.0062 | 0.0292 |
Cytoplasmic Foci | – | – | – | – | – | – | 0.0083 | 0.0075 | 0.022 | 0.0054 | 0.0119 | 0.008 | 0.0139 | 0.0077 | 0.0107 | 0.0036 | 0.0071 | 0.0125 |
Eisosomes | – | – | – | – | – | – | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | – | – | – | – | – | – | 0.0227 | 0.0141 | 0.0068 | 0.0048 | 0.0057 | 0.0085 | 0.0235 | 0.0112 | 0.0183 | 0.0024 | 0.003 | 0.0085 |
Endosome | – | – | – | – | – | – | 0.2321 | 0.1448 | 0.225 | 0.1877 | 0.1735 | 0.2015 | 0.256 | 0.1122 | 0.2252 | 0.1562 | 0.2149 | 0.1984 |
Golgi | – | – | – | – | – | – | 0.009 | 0.0025 | 0.0164 | 0.0026 | 0.0122 | 0.0054 | 0.0029 | 0.0019 | 0.0037 | 0.0012 | 0.003 | 0.0049 |
Lipid Particles | – | – | – | – | – | – | 0.019 | 0.0053 | 0.0081 | 0.0204 | 0.0213 | 0.0086 | 0.0046 | 0.0037 | 0.0044 | 0.003 | 0.0064 | 0.0094 |
Mitochondria | – | – | – | – | – | – | 0.0079 | 0.0032 | 0.0184 | 0.005 | 0.0046 | 0.0159 | 0.0011 | 0.0009 | 0.0046 | 0.0004 | 0.0007 | 0.0016 |
None | – | – | – | – | – | – | 0.0181 | 0.008 | 0.0123 | 0.0002 | 0.0018 | 0.0011 | 0.0013 | 0.028 | 0.0023 | 0.0001 | 0.0002 | 0.0025 |
Nuclear Periphery | – | – | – | – | – | – | 0.0143 | 0.0082 | 0.0084 | 0.0026 | 0.008 | 0.0046 | 0.0059 | 0.0097 | 0.0087 | 0.0023 | 0.0033 | 0.0079 |
Nucleolus | – | – | – | – | – | – | 0.0032 | 0.0025 | 0.0019 | 0.0023 | 0.0036 | 0.0015 | 0.0014 | 0.0015 | 0.0017 | 0.0029 | 0.0016 | 0.0031 |
Nucleus | – | – | – | – | – | – | 0.0169 | 0.0075 | 0.0039 | 0.0035 | 0.0054 | 0.0033 | 0.0049 | 0.008 | 0.0063 | 0.0036 | 0.0023 | 0.0208 |
Peroxisomes | – | – | – | – | – | – | 0.0021 | 0.0003 | 0.0083 | 0.0003 | 0.0099 | 0.0007 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0003 |
Punctate Nuclear | – | – | – | – | – | – | 0.0009 | 0.0003 | 0.0017 | 0.0001 | 0.0035 | 0.0001 | 0.0002 | 0.0011 | 0.0002 | 0.0001 | 0.0001 | 0.0003 |
Vacuole | – | – | – | – | – | – | 0.5352 | 0.7056 | 0.5685 | 0.6953 | 0.6292 | 0.6513 | 0.5745 | 0.7163 | 0.5867 | 0.7569 | 0.6734 | 0.6394 |
Vacuole Periphery | – | – | – | – | – | – | 0.0678 | 0.0512 | 0.048 | 0.0494 | 0.0673 | 0.0687 | 0.0529 | 0.0409 | 0.0475 | 0.0507 | 0.0705 | 0.0521 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.0562 | 15.3821 | 13.7921 | 19.356 | 12.5492 | 12.1816 | 20.2511 | 24.9306 | 21.5751 | 21.6506 |
Translational Efficiency | 0.9988 | 0.757 | 0.9406 | 0.6896 | 0.844 | 1.0387 | 0.7868 | 0.4487 | 0.4869 | 0.4966 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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