Standard name | |
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Human Ortholog | |
Description | Subunit of the Set3C deacetylase complex; interacts directly with the Set3C subunit, Sif2p; putative DNA-binding protein; mutant has increased aneuploidy tolerance; relocalizes to the cytosol in response to hypoxia |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 |
Bud | 0 | 0 | 1 | 1 | 3 | 4 | 5 | 7 | 8 | 7 | 2 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 3 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 |
Bud Site | 1 | 0 | 0 | 3 | 9 | 10 | 17 | 32 | 23 | 16 | 7 | 22 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 14 | 2 | 7 | 9 | 12 | 16 | 11 | 21 | 20 | 24 | 10 | 18 | 3 | 7 | 17 | 34 | 22 | 28 | 3 | 0 | 0 | 0 | 1 | 2 |
Endoplasmic Reticulum | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 8 | 4 | 5 | 1 | 0 | 2 | 0 | 1 | 0 |
Endosome | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7 | 3 | 1 | 4 | 0 | 3 | 0 | 3 | 7 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 5 | 0 | 0 | 0 | 0 | 1 | 5 |
Mitochondria | 4 | 59 | 155 | 140 | 140 | 248 | 332 | 327 | 303 | 302 | 140 | 221 | 6 | 12 | 32 | 24 | 10 | 18 | 7 | 3 | 5 | 0 | 3 | 4 |
Nucleus | 270 | 86 | 199 | 159 | 191 | 251 | 335 | 338 | 255 | 284 | 123 | 194 | 39 | 87 | 84 | 112 | 122 | 84 | 191 | 86 | 172 | 42 | 106 | 97 |
Nuclear Periphery | 2 | 0 | 1 | 2 | 2 | 0 | 4 | 3 | 4 | 8 | 5 | 4 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 1 |
Nucleolus | 50 | 11 | 3 | 5 | 4 | 10 | 32 | 55 | 37 | 58 | 27 | 62 | 11 | 16 | 13 | 9 | 3 | 3 | 94 | 15 | 41 | 9 | 27 | 31 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 6 | 0 | 9 | 5 | 6 | 2 | 10 | 0 | 1 | 2 | 5 | 2 | 0 | 0 | 0 | 2 | 4 | 5 | 7 | 2 | 8 | 2 | 5 | 6 |
Vac/Vac Membrane | 2 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 1 | 1 | 6 | 4 | 5 | 14 | 10 | 18 | 0 | 7 | 17 |
Unique Cell Count | 306 | 107 | 254 | 197 | 247 | 346 | 451 | 464 | 385 | 418 | 200 | 307 | 48 | 98 | 110 | 168 | 158 | 129 | 337 | 127 | 261 | 59 | 168 | 184 |
Labelled Cell Count | 352 | 158 | 377 | 325 | 368 | 543 | 747 | 785 | 653 | 703 | 321 | 529 | 61 | 123 | 147 | 204 | 175 | 154 | 337 | 127 | 261 | 59 | 168 | 184 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.2 | 3.9 | 3.6 | 3.6 | 3.8 | 3.6 | 3.2 | 3.4 | 3.2 | 3.3 | 3.4 | 3.2 | 5.3 | 5.0 | 4.4 | 7.4 | 7.4 | 8.6 | 4.7 | 5.6 | 6.3 |
Std Deviation (1e-4) | 0.9 | 0.6 | 0.7 | 1.3 | 1.2 | 1.2 | 0.8 | 1.0 | 1.3 | 1.3 | 1.5 | 1.4 | 1.2 | 0.9 | 0.9 | 2.2 | 2.5 | 3.2 | 0.7 | 1.9 | 2.2 |
Intensity Change (Log2) | – | – | – | -0.0 | 0.07 | -0.03 | -0.16 | -0.09 | -0.17 | -0.14 | -0.09 | -0.18 | 0.55 | 0.46 | 0.27 | 1.03 | 1.03 | 1.25 | 0.37 | 0.64 | 0.8 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.6104 | 1.8795 | 1.7381 | 1.7272 | 0.9734 | 1.9018 | 2.8661 | 2.5921 | 2.6401 | 2.1154 | 2.3141 | 2.5375 | 3.4787 | 3.5894 | 3.5614 | 3.9472 | 3.574 | 3.2423 |
Actin | 0.0073 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0138 | 0.0004 | 0.0069 | 0.0331 | 0.0063 | 0.0051 | 0.0021 | 0 | 0 | 0.0088 | 0.001 | 0.0005 |
Bud | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0.0001 | 0.0005 | 0.0003 | 0.0002 | 0.0001 | 0.0009 | 0.0002 | 0.0001 | 0 | 0 | 0.0002 | 0.0001 | 0 |
Bud Neck | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0.0069 | 0.0023 | 0.0014 | 0.0004 | 0.0004 | 0.0005 | 0.0013 | 0.0011 | 0.0003 | 0.0001 | 0.0001 | 0.0012 | 0.0022 | 0.001 |
Bud Periphery | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0004 | 0.0001 | 0.0006 | 0.0008 | 0.0005 | 0.0001 | 0.0034 | 0.0006 | 0.0003 | 0 | 0 | 0.0002 | 0.0003 | 0.0001 |
Bud Site | 0.0004 | 0.0011 | 0.0001 | 0.0001 | 0.0008 | 0.0002 | 0.0047 | 0.0006 | 0.0008 | 0.0007 | 0.0017 | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0.0013 | 0.0005 | 0.0001 |
Cell Periphery | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 |
Cytoplasm | 0.0013 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0108 | 0.0001 | 0.0023 | 0.0001 | 0.0015 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0011 | 0.0017 | 0 |
Cytoplasmic Foci | 0.0046 | 0.0001 | 0 | 0 | 0 | 0 | 0.0202 | 0.0001 | 0.0122 | 0.0027 | 0.0042 | 0.0028 | 0.0025 | 0 | 0 | 0.0067 | 0.0126 | 0.0002 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0007 | 0 | 0.0001 | 0 | 0 | 0 | 0.0076 | 0.0001 | 0.0003 | 0 | 0.0015 | 0.0011 | 0.0001 | 0 | 0 | 0.0002 | 0.0006 | 0 |
Endosome | 0.0067 | 0.0001 | 0.0006 | 0 | 0.0002 | 0.0001 | 0.0427 | 0.0003 | 0.0044 | 0.0005 | 0.0166 | 0.0068 | 0.0064 | 0 | 0 | 0.0056 | 0.0151 | 0.0013 |
Golgi | 0.0016 | 0 | 0.0001 | 0 | 0 | 0 | 0.0171 | 0.0001 | 0.0062 | 0.0017 | 0.0163 | 0.0045 | 0.0025 | 0 | 0 | 0.0048 | 0.0008 | 0.0027 |
Lipid Particles | 0.0045 | 0 | 0.0002 | 0 | 0 | 0 | 0.0054 | 0.0003 | 0.009 | 0.0017 | 0.0068 | 0.0102 | 0.0138 | 0 | 0 | 0.0069 | 0.0072 | 0.0002 |
Mitochondria | 0.0007 | 0.0002 | 0.0074 | 0.0001 | 0.0006 | 0.0001 | 0.0042 | 0.0035 | 0.0046 | 0.0004 | 0.054 | 0.012 | 0.0195 | 0.0001 | 0.0002 | 0.0016 | 0.0021 | 0.0067 |
None | 0.0039 | 0.0002 | 0.0008 | 0.0001 | 0.0001 | 0.0001 | 0.0058 | 0.0001 | 0.0002 | 0.0001 | 0.0007 | 0.0001 | 0.0002 | 0 | 0 | 0.0146 | 0.0037 | 0 |
Nuclear Periphery | 0.003 | 0.0033 | 0.0026 | 0.0014 | 0.0005 | 0.0011 | 0.0174 | 0.0005 | 0.0016 | 0.0003 | 0.0306 | 0.0062 | 0.0015 | 0.0007 | 0.0005 | 0.0034 | 0.0164 | 0.0001 |
Nucleolus | 0.0209 | 0.0554 | 0.0288 | 0.0215 | 0.2943 | 0.1187 | 0.0432 | 0.0392 | 0.0371 | 0.0191 | 0.1749 | 0.0915 | 0.0415 | 0.039 | 0.0351 | 0.0558 | 0.1839 | 0.1008 |
Nucleus | 0.9319 | 0.9214 | 0.953 | 0.9758 | 0.6912 | 0.853 | 0.7737 | 0.9486 | 0.8948 | 0.9333 | 0.6587 | 0.8461 | 0.9015 | 0.958 | 0.9625 | 0.8696 | 0.6945 | 0.8786 |
Peroxisomes | 0.0014 | 0 | 0.0001 | 0 | 0 | 0 | 0.016 | 0.0001 | 0.0136 | 0.0039 | 0.0048 | 0.0063 | 0.0035 | 0 | 0 | 0.007 | 0.0011 | 0.0001 |
Punctate Nuclear | 0.01 | 0.0167 | 0.0047 | 0.0006 | 0.0033 | 0.0239 | 0.011 | 0.0029 | 0.0029 | 0.0015 | 0.0077 | 0.0034 | 0.0027 | 0.0017 | 0.0015 | 0.0104 | 0.0546 | 0.0072 |
Vacuole | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0007 | 0.0001 | 0.0018 | 0.0009 | 0.0011 | 0 | 0.0046 | 0.0009 | 0.0004 | 0 | 0 | 0.0002 | 0.0008 | 0.0001 |
Vacuole Periphery | 0.0002 | 0.0001 | 0.0005 | 0 | 0.0002 | 0 | 0.0019 | 0.0004 | 0.0007 | 0 | 0.003 | 0.0007 | 0.0004 | 0 | 0 | 0.0001 | 0.0005 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 17.8355 | 17.6856 | 16.1061 | 20.1036 | 20.7927 | 14.0808 | 24.4522 | 26.6069 | 23.434 | 25.1957 |
Translational Efficiency | 0.6073 | 0.703 | 0.6375 | 0.4256 | 0.4549 | 0.7373 | 0.4495 | 0.4105 | 0.4492 | 0.4715 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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