Standard name | |
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Human Ortholog | |
Description | Protein of the endoplasmic reticulum; required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 3 | 11 | 8 | 9 | 4 | 4 | 6 | 4 | 3 | 7 | 6 | 10 | 29 | 35 | 24 | 10 | 12 | 8 | 3 | 5 | 7 | 5 | 8 | 13 |
Bud | 0 | 0 | 3 | 7 | 3 | 29 | 12 | 17 | 28 | 19 | 40 | 30 | 2 | 2 | 2 | 0 | 4 | 3 | 1 | 0 | 1 | 1 | 3 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 1 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 8 | 4 | 14 | 16 | 12 | 16 | 14 | 22 | 24 | 20 | 24 | 5 | 3 | 8 | 10 | 11 | 13 | 0 | 0 | 1 | 2 | 2 | 3 |
Cytoplasm | 56 | 93 | 85 | 162 | 200 | 243 | 254 | 314 | 313 | 269 | 224 | 280 | 91 | 76 | 148 | 65 | 73 | 61 | 12 | 23 | 17 | 21 | 35 | 45 |
Endoplasmic Reticulum | 196 | 225 | 203 | 114 | 224 | 152 | 258 | 128 | 151 | 177 | 97 | 193 | 229 | 237 | 215 | 2 | 3 | 2 | 327 | 321 | 358 | 288 | 340 | 356 |
Endosome | 4 | 2 | 11 | 21 | 21 | 12 | 7 | 41 | 17 | 16 | 16 | 9 | 11 | 27 | 45 | 5 | 16 | 8 | 1 | 1 | 1 | 2 | 3 | 3 |
Golgi | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 5 | 1 | 0 | 3 | 4 | 0 | 1 | 1 | 4 | 3 | 2 |
Mitochondria | 8 | 1 | 51 | 109 | 174 | 313 | 490 | 262 | 353 | 366 | 358 | 560 | 0 | 0 | 4 | 10 | 16 | 9 | 7 | 6 | 7 | 26 | 30 | 27 |
Nucleus | 0 | 0 | 0 | 0 | 3 | 8 | 13 | 17 | 8 | 9 | 8 | 11 | 1 | 2 | 1 | 2 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 7 | 2 | 5 | 4 | 6 | 17 | 38 | 18 | 10 | 34 | 25 | 21 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 2 | 0 | 0 | 3 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 5 | 2 | 0 | 1 | 10 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 2 | 0 | 0 | 4 | 6 | 1 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 0 |
Vac/Vac Membrane | 62 | 33 | 49 | 125 | 97 | 147 | 197 | 206 | 199 | 276 | 129 | 150 | 8 | 11 | 38 | 22 | 68 | 47 | 2 | 6 | 3 | 14 | 16 | 28 |
Unique Cell Count | 299 | 326 | 353 | 471 | 635 | 736 | 1048 | 865 | 861 | 968 | 715 | 1052 | 339 | 343 | 450 | 116 | 186 | 144 | 362 | 373 | 407 | 376 | 452 | 503 |
Labelled Cell Count | 339 | 376 | 420 | 567 | 750 | 941 | 1296 | 1026 | 1109 | 1202 | 928 | 1299 | 379 | 402 | 497 | 128 | 211 | 158 | 362 | 373 | 407 | 376 | 452 | 503 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.1 | 8.9 | 7.2 | 7.0 | 6.2 | 5.9 | 5.4 | 6.0 | 5.7 | 5.5 | 5.5 | 5.6 | 8.6 | 8.3 | 8.9 | 8.4 | 8.8 | 8.7 | 9.5 | 9.4 | 9.8 |
Std Deviation (1e-4) | 1.5 | 1.5 | 1.4 | 1.6 | 1.4 | 1.4 | 1.1 | 1.3 | 1.3 | 1.2 | 1.4 | 1.3 | 1.3 | 1.2 | 1.4 | 1.4 | 1.6 | 1.6 | 1.6 | 1.5 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.03 | -0.21 | -0.29 | -0.41 | -0.26 | -0.33 | -0.37 | -0.38 | -0.36 | 0.27 | 0.21 | 0.31 | 0.23 | 0.29 | 0.28 | 0.41 | 0.39 | 0.45 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.7 | 4.3 | 2.2 | 0 | 2.4 | 0 |
Bud | 0 | 0 | 2.8 | 0 | 1.4 | 2.4 | 1.4 | 3.7 | 2.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.8 | 1.5 | 0.6 | 0.5 | 0.6 | 1.6 | 1.5 | 1.7 | 1.3 | 0 | 0 | 0.7 | 0 | 3.2 | 0 |
Cytoplasm | 3.2 | 2.5 | 3.0 | 0.1 | 4.1 | 4.1 | 1.3 | 2.5 | 0.9 | 0.8 | -0.6 | 2.7 | 6.4 | 3.7 | 4.1 |
Endoplasmic Reticulum | -9.7 | -6.8 | -12.1 | -11.4 | -15.2 | -13.9 | -13.9 | -15.0 | -14.2 | 2.7 | 3.2 | -2.7 | -10.5 | -12.7 | -11.5 |
Endosome | 1.0 | 0.2 | -1.6 | 0 | 1.3 | -1.2 | -1.7 | -0.9 | -3.1 | 0.1 | 2.8 | 3.8 | 0 | 2.8 | 1.3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.1 | 4.7 | 9.2 | 10.8 | 5.7 | 8.9 | 8.1 | 11.3 | 12.7 | -7.3 | -7.3 | -7.5 | -1.6 | -2.0 | -2.5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 1.0 | 2.1 | 0.8 | 0 | 2.0 | 1.9 | 0.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 4.4 | 0.6 | 2.4 | 2.1 | 3.9 | 3.6 | 5.5 | 1.7 | 0.2 | -5.5 | -5.0 | -2.5 | 1.3 | 6.1 | 4.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.8026 | 4.1719 | 3.9596 | 3.3406 | 3.4454 | 3.9029 | 5.2192 | 6.1034 | 5.5089 | 4.9187 | 4.8442 | 5.3474 | 3.0139 | 3.4651 | 3.0347 | 3.4493 | 2.8607 | 3.2047 |
Actin | 0.012 | 0.0026 | 0.0167 | 0.0187 | 0.0017 | 0.0048 | 0.0064 | 0.0008 | 0.0106 | 0.0038 | 0.0049 | 0.0076 | 0.011 | 0.0028 | 0.0122 | 0.0043 | 0.0085 | 0.0018 |
Bud | 0.0044 | 0.0108 | 0.0146 | 0.0027 | 0.0002 | 0.0058 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0 |
Bud Neck | 0.0087 | 0.0004 | 0.0017 | 0.0009 | 0.0005 | 0.0037 | 0.0011 | 0.0001 | 0.0004 | 0.0003 | 0.0025 | 0.0009 | 0.0011 | 0.0003 | 0.0014 | 0.0003 | 0.0011 | 0.0003 |
Bud Periphery | 0.0026 | 0.0016 | 0.0037 | 0.0011 | 0.0002 | 0.0024 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0.0006 | 0.0003 | 0.0001 |
Bud Site | 0.0087 | 0.0308 | 0.0134 | 0.001 | 0.0005 | 0.0012 | 0.0018 | 0.0006 | 0.0014 | 0.0001 | 0.0006 | 0.0001 | 0.0022 | 0.001 | 0.0011 | 0.0005 | 0.0002 | 0.0001 |
Cell Periphery | 0.0015 | 0.001 | 0.0017 | 0.0003 | 0.0002 | 0.0004 | 0.0009 | 0.0009 | 0.001 | 0.0005 | 0.0012 | 0.0003 | 0.0006 | 0.0006 | 0.0004 | 0.0003 | 0.0003 | 0.0001 |
Cytoplasm | 0.1263 | 0.2143 | 0.1411 | 0.1194 | 0.1504 | 0.2205 | 0.1095 | 0.1626 | 0.1648 | 0.0957 | 0.1644 | 0.1535 | 0.1062 | 0.175 | 0.1476 | 0.1206 | 0.0478 | 0.1333 |
Cytoplasmic Foci | 0.0356 | 0.0164 | 0.0223 | 0.0269 | 0.0303 | 0.0227 | 0.017 | 0.0109 | 0.0128 | 0.005 | 0.0074 | 0.016 | 0.0205 | 0.0095 | 0.012 | 0.0052 | 0.0067 | 0.0048 |
Eisosomes | 0.0002 | 0.0001 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0006 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.4992 | 0.5746 | 0.6061 | 0.5886 | 0.5593 | 0.561 | 0.7349 | 0.6908 | 0.6871 | 0.8182 | 0.7573 | 0.7253 | 0.7088 | 0.7362 | 0.7299 | 0.7544 | 0.8148 | 0.7736 |
Endosome | 0.0871 | 0.0635 | 0.0466 | 0.0785 | 0.0751 | 0.0563 | 0.025 | 0.0289 | 0.0321 | 0.0315 | 0.0209 | 0.034 | 0.052 | 0.0282 | 0.0293 | 0.0498 | 0.0249 | 0.027 |
Golgi | 0.0259 | 0.0054 | 0.0151 | 0.0248 | 0.0074 | 0.0063 | 0.0042 | 0.005 | 0.0086 | 0.0065 | 0.0022 | 0.0035 | 0.0094 | 0.0032 | 0.0069 | 0.0066 | 0.0082 | 0.0034 |
Lipid Particles | 0.0249 | 0.0069 | 0.0166 | 0.0198 | 0.0094 | 0.0071 | 0.0131 | 0.019 | 0.0143 | 0.0033 | 0.0054 | 0.0085 | 0.0188 | 0.0079 | 0.0071 | 0.0034 | 0.0305 | 0.0031 |
Mitochondria | 0.0246 | 0.0018 | 0.0183 | 0.0142 | 0.0814 | 0.0063 | 0.0035 | 0.003 | 0.0013 | 0.0016 | 0.0005 | 0.0009 | 0.0161 | 0.0009 | 0.0022 | 0.002 | 0.0019 | 0.0019 |
None | 0.0416 | 0.0233 | 0.0198 | 0.0218 | 0.0047 | 0.0388 | 0.0549 | 0.0436 | 0.032 | 0.0089 | 0.01 | 0.0247 | 0.0111 | 0.0066 | 0.0206 | 0.02 | 0.0011 | 0.0098 |
Nuclear Periphery | 0.0315 | 0.0207 | 0.0185 | 0.0235 | 0.026 | 0.0271 | 0.0136 | 0.0157 | 0.0166 | 0.017 | 0.0109 | 0.0141 | 0.0207 | 0.0179 | 0.0199 | 0.0211 | 0.0362 | 0.0299 |
Nucleolus | 0.003 | 0.0005 | 0.0035 | 0.0003 | 0.0007 | 0.0013 | 0.0002 | 0.0002 | 0.0003 | 0 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0001 |
Nucleus | 0.0083 | 0.0072 | 0.0114 | 0.0063 | 0.0296 | 0.0119 | 0.0019 | 0.0069 | 0.0046 | 0.0022 | 0.0026 | 0.0026 | 0.0021 | 0.0021 | 0.0018 | 0.0026 | 0.0039 | 0.0035 |
Peroxisomes | 0.0222 | 0.0008 | 0.0104 | 0.0405 | 0.0068 | 0.003 | 0.0042 | 0.0038 | 0.0017 | 0.0001 | 0.0003 | 0.0011 | 0.0051 | 0.0004 | 0.0012 | 0.0003 | 0.0029 | 0.0025 |
Punctate Nuclear | 0.0082 | 0.0046 | 0.0098 | 0.0016 | 0.0034 | 0.0107 | 0.0033 | 0.0008 | 0.0035 | 0.0003 | 0.0006 | 0.0019 | 0.0012 | 0.0012 | 0.0006 | 0.001 | 0.0049 | 0.0008 |
Vacuole | 0.0184 | 0.0109 | 0.006 | 0.0065 | 0.0078 | 0.0068 | 0.0031 | 0.0052 | 0.0054 | 0.0038 | 0.0071 | 0.004 | 0.0088 | 0.005 | 0.0045 | 0.0052 | 0.004 | 0.003 |
Vacuole Periphery | 0.0051 | 0.0017 | 0.0023 | 0.0022 | 0.0045 | 0.0019 | 0.0008 | 0.0007 | 0.0011 | 0.001 | 0.0005 | 0.0008 | 0.0029 | 0.0009 | 0.0008 | 0.0012 | 0.0016 | 0.0011 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 43.1499 | 35.8181 | 36.1196 | 35.8127 | 34.6331 | 42.0992 | 45.0374 | 36.038 | 40.8946 | 44.7342 |
Translational Efficiency | 0.6035 | 0.5782 | 0.6475 | 0.6433 | 0.652 | 0.6998 | 0.5753 | 0.5688 | 0.6236 | 0.5691 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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