Standard name
Human Ortholog
Description Protein of the endoplasmic reticulum; required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.1 0.05 0.09 0.06 0.06 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.06 0.09 0 0 0 0 0 0
Cytoplasm 0.19 0.29 0.24 0.34 0.31 0.33 0.24 0.36 0.36 0.28 0.31 0.27 0.27 0.22 0.33 0.56 0.39 0.42 0 0.06 0 0.06 0.08 0.09
Endoplasmic Reticulum 0.66 0.69 0.58 0.24 0.35 0.21 0.25 0.15 0.18 0.18 0.14 0.18 0.68 0.69 0.48 0 0 0 0.9 0.86 0.88 0.77 0.75 0.71
Endosome 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.08 0.1 0 0.09 0.06 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.14 0.23 0.27 0.43 0.47 0.3 0.41 0.38 0.5 0.53 0 0 0 0.09 0.09 0.06 0 0 0 0.07 0.07 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.21 0.1 0.14 0.27 0.15 0.2 0.19 0.24 0.23 0.29 0.18 0.14 0 0 0.08 0.19 0.37 0.33 0 0 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 11 8 9 4 4 6 4 3 7 6 10 29 35 24 10 12 8 3 5 7 5 8 13
Bud 0 0 3 7 3 29 12 17 28 19 40 30 2 2 2 0 4 3 1 0 1 1 3 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 2 4
Bud Site 0 0 0 0 0 3 1 0 1 3 1 1 0 0 0 0 0 0
Cell Periphery 3 8 4 14 16 12 16 14 22 24 20 24 5 3 8 10 11 13 0 0 1 2 2 3
Cytoplasm 56 93 85 162 200 243 254 314 313 269 224 280 91 76 148 65 73 61 12 23 17 21 35 45
Endoplasmic Reticulum 196 225 203 114 224 152 258 128 151 177 97 193 229 237 215 2 3 2 327 321 358 288 340 356
Endosome 4 2 11 21 21 12 7 41 17 16 16 9 11 27 45 5 16 8 1 1 1 2 3 3
Golgi 0 1 1 1 1 0 0 0 0 0 0 0 3 5 1 0 3 4 0 1 1 4 3 2
Mitochondria 8 1 51 109 174 313 490 262 353 366 358 560 0 0 4 10 16 9 7 6 7 26 30 27
Nucleus 0 0 0 0 3 8 13 17 8 9 8 11 1 2 1 2 3 1 0 0 0 0 0 0
Nuclear Periphery 7 2 5 4 6 17 38 18 10 34 25 21 0 0 0 1 1 0 2 0 2 0 0 3
Nucleolus 0 0 0 0 1 1 3 5 2 0 1 10 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 1 0 0 0 0 0 0 1 0 0 0 4 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 0 2 2 2 0 0 4 6 1 1 2 0 0 0 1 0 0
Vac/Vac Membrane 62 33 49 125 97 147 197 206 199 276 129 150 8 11 38 22 68 47 2 6 3 14 16 28
Unique Cell Count 299 326 353 471 635 736 1048 865 861 968 715 1052 339 343 450 116 186 144 362 373 407 376 452 503
Labelled Cell Count 339 376 420 567 750 941 1296 1026 1109 1202 928 1299 379 402 497 128 211 158 362 373 407 376 452 503


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.1 8.9 7.2 7.0 6.2 5.9 5.4 6.0 5.7 5.5 5.5 5.6 8.6 8.3 8.9 8.4 8.8 8.7 9.5 9.4 9.8
Std Deviation (1e-4) 1.5 1.5 1.4 1.6 1.4 1.4 1.1 1.3 1.3 1.2 1.4 1.3 1.3 1.2 1.4 1.4 1.6 1.6 1.6 1.5 1.5
Intensity Change (Log2) -0.03 -0.21 -0.29 -0.41 -0.26 -0.33 -0.37 -0.38 -0.36 0.27 0.21 0.31 0.23 0.29 0.28 0.41 0.39 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.4 0 0 0 0 0 0 0 0 3.7 4.3 2.2 0 2.4 0
Bud 0 0 2.8 0 1.4 2.4 1.4 3.7 2.1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.8 1.5 0.6 0.5 0.6 1.6 1.5 1.7 1.3 0 0 0.7 0 3.2 0
Cytoplasm 3.2 2.5 3.0 0.1 4.1 4.1 1.3 2.5 0.9 0.8 -0.6 2.7 6.4 3.7 4.1
Endoplasmic Reticulum -9.7 -6.8 -12.1 -11.4 -15.2 -13.9 -13.9 -15.0 -14.2 2.7 3.2 -2.7 -10.5 -12.7 -11.5
Endosome 1.0 0.2 -1.6 0 1.3 -1.2 -1.7 -0.9 -3.1 0.1 2.8 3.8 0 2.8 1.3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.1 4.7 9.2 10.8 5.7 8.9 8.1 11.3 12.7 -7.3 -7.3 -7.5 -1.6 -2.0 -2.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 1.0 2.1 0.8 0 2.0 1.9 0.7 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 4.4 0.6 2.4 2.1 3.9 3.6 5.5 1.7 0.2 -5.5 -5.0 -2.5 1.3 6.1 4.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.8026 4.1719 3.9596 3.3406 3.4454 3.9029 5.2192 6.1034 5.5089 4.9187 4.8442 5.3474 3.0139 3.4651 3.0347 3.4493 2.8607 3.2047
Actin 0.012 0.0026 0.0167 0.0187 0.0017 0.0048 0.0064 0.0008 0.0106 0.0038 0.0049 0.0076 0.011 0.0028 0.0122 0.0043 0.0085 0.0018
Bud 0.0044 0.0108 0.0146 0.0027 0.0002 0.0058 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0003 0.0001 0.0001 0.0003 0.0001 0
Bud Neck 0.0087 0.0004 0.0017 0.0009 0.0005 0.0037 0.0011 0.0001 0.0004 0.0003 0.0025 0.0009 0.0011 0.0003 0.0014 0.0003 0.0011 0.0003
Bud Periphery 0.0026 0.0016 0.0037 0.0011 0.0002 0.0024 0.0002 0.0002 0.0001 0.0001 0.0003 0.0001 0.0004 0.0002 0.0002 0.0006 0.0003 0.0001
Bud Site 0.0087 0.0308 0.0134 0.001 0.0005 0.0012 0.0018 0.0006 0.0014 0.0001 0.0006 0.0001 0.0022 0.001 0.0011 0.0005 0.0002 0.0001
Cell Periphery 0.0015 0.001 0.0017 0.0003 0.0002 0.0004 0.0009 0.0009 0.001 0.0005 0.0012 0.0003 0.0006 0.0006 0.0004 0.0003 0.0003 0.0001
Cytoplasm 0.1263 0.2143 0.1411 0.1194 0.1504 0.2205 0.1095 0.1626 0.1648 0.0957 0.1644 0.1535 0.1062 0.175 0.1476 0.1206 0.0478 0.1333
Cytoplasmic Foci 0.0356 0.0164 0.0223 0.0269 0.0303 0.0227 0.017 0.0109 0.0128 0.005 0.0074 0.016 0.0205 0.0095 0.012 0.0052 0.0067 0.0048
Eisosomes 0.0002 0.0001 0.0004 0.0003 0.0001 0.0001 0.0003 0.0001 0.0001 0.0002 0 0.0001 0.0006 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.4992 0.5746 0.6061 0.5886 0.5593 0.561 0.7349 0.6908 0.6871 0.8182 0.7573 0.7253 0.7088 0.7362 0.7299 0.7544 0.8148 0.7736
Endosome 0.0871 0.0635 0.0466 0.0785 0.0751 0.0563 0.025 0.0289 0.0321 0.0315 0.0209 0.034 0.052 0.0282 0.0293 0.0498 0.0249 0.027
Golgi 0.0259 0.0054 0.0151 0.0248 0.0074 0.0063 0.0042 0.005 0.0086 0.0065 0.0022 0.0035 0.0094 0.0032 0.0069 0.0066 0.0082 0.0034
Lipid Particles 0.0249 0.0069 0.0166 0.0198 0.0094 0.0071 0.0131 0.019 0.0143 0.0033 0.0054 0.0085 0.0188 0.0079 0.0071 0.0034 0.0305 0.0031
Mitochondria 0.0246 0.0018 0.0183 0.0142 0.0814 0.0063 0.0035 0.003 0.0013 0.0016 0.0005 0.0009 0.0161 0.0009 0.0022 0.002 0.0019 0.0019
None 0.0416 0.0233 0.0198 0.0218 0.0047 0.0388 0.0549 0.0436 0.032 0.0089 0.01 0.0247 0.0111 0.0066 0.0206 0.02 0.0011 0.0098
Nuclear Periphery 0.0315 0.0207 0.0185 0.0235 0.026 0.0271 0.0136 0.0157 0.0166 0.017 0.0109 0.0141 0.0207 0.0179 0.0199 0.0211 0.0362 0.0299
Nucleolus 0.003 0.0005 0.0035 0.0003 0.0007 0.0013 0.0002 0.0002 0.0003 0 0.0003 0.0001 0.0002 0.0001 0 0.0001 0.0003 0.0001
Nucleus 0.0083 0.0072 0.0114 0.0063 0.0296 0.0119 0.0019 0.0069 0.0046 0.0022 0.0026 0.0026 0.0021 0.0021 0.0018 0.0026 0.0039 0.0035
Peroxisomes 0.0222 0.0008 0.0104 0.0405 0.0068 0.003 0.0042 0.0038 0.0017 0.0001 0.0003 0.0011 0.0051 0.0004 0.0012 0.0003 0.0029 0.0025
Punctate Nuclear 0.0082 0.0046 0.0098 0.0016 0.0034 0.0107 0.0033 0.0008 0.0035 0.0003 0.0006 0.0019 0.0012 0.0012 0.0006 0.001 0.0049 0.0008
Vacuole 0.0184 0.0109 0.006 0.0065 0.0078 0.0068 0.0031 0.0052 0.0054 0.0038 0.0071 0.004 0.0088 0.005 0.0045 0.0052 0.004 0.003
Vacuole Periphery 0.0051 0.0017 0.0023 0.0022 0.0045 0.0019 0.0008 0.0007 0.0011 0.001 0.0005 0.0008 0.0029 0.0009 0.0008 0.0012 0.0016 0.0011

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 43.1499 35.8181 36.1196 35.8127 34.6331 42.0992 45.0374 36.038 40.8946 44.7342
Translational Efficiency 0.6035 0.5782 0.6475 0.6433 0.652 0.6998 0.5753 0.5688 0.6236 0.5691

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Protein of the endoplasmic reticulum; required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1
Localization
Cell Percentages ER (95%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Per1

Per1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Per1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available