Standard name
Human Ortholog
Description Methyltransferase that methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern; functional homolog of human WBSCR22
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.1 0.09 0.08
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0.1 0.1 0.1 0.05 0.07 0.07 0.08 0.07 0.1 0.1 0.09 0.16 0.25 0.25 0.13 0.18 0.2 0.07 0.05 0 0 0.09 0.13
Endoplasmic Reticulum – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome – 0.1 0.28 0.23 0 0 0 0 0 0 0 0 0.12 0.17 0.06 0.2 0.14 0.08 0.15 0.11 0.13 0.11 0.11 0.15
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.09 0 0 0 0 0 0
Mitochondria – 0 0 0 0.06 0.34 0.51 0.4 0.44 0.52 0.45 0.53 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus – 0.08 0.08 0.11 0.59 0.5 0.3 0.31 0.43 0.33 0.4 0.35 0.25 0.1 0.3 0.08 0.09 0.06 0.09 0.26 0.19 0.22 0.19 0.15
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0.32 0.33 0.41 0.28 0.18 0.19 0.21 0.2 0.17 0.18 0.18 0.15 0.15 0.08 0.19 0.22 0.19 0.2 0.28 0.24 0.25 0.11 0.11
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.33 0.11 0.26 0.11 0.09 0.09
Vac/Vac Membrane – 0.53 0.31 0.3 0.15 0.12 0.18 0.14 0.12 0.13 0.1 0.1 0.45 0.46 0.39 0.61 0.5 0.55 0 0.05 0.05 0.1 0.2 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 1 0 1 0 0 0 0 0 0 0 0 1 0 1 1 3 1 1 0 0 0 2 0
Bud – 0 1 2 1 4 6 8 4 9 9 13 0 0 0 0 0 0 1 6 2 9 18 21
Bud Neck – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 1 1
Bud Site – 0 0 0 0 6 8 3 9 10 4 13 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 1 0 0 0 0 0 0
Cytoplasm – 6 4 16 13 23 26 49 24 41 39 46 18 54 65 37 53 58 4 4 2 4 18 32
Endoplasmic Reticulum – 0 0 0 0 0 0 0 0 0 0 1 0 1 0 7 10 13 0 0 1 1 2 4
Endosome – 6 11 36 8 5 12 23 6 10 5 15 13 36 16 58 41 23 8 10 11 10 23 37
Golgi – 1 0 4 0 0 0 0 0 0 0 0 1 8 1 8 19 28 0 1 0 0 0 2
Mitochondria – 2 1 4 15 114 194 229 141 215 186 260 0 0 0 2 12 5 0 1 0 0 2 1
Nucleus – 5 3 17 157 169 116 179 139 136 164 174 28 21 77 24 28 18 5 25 16 21 38 37
Nuclear Periphery – 0 0 0 1 2 1 5 0 6 0 3 0 0 1 0 0 0 0 0 1 0 2 3
Nucleolus – 19 13 65 75 60 74 123 65 69 74 90 17 33 20 55 65 56 11 27 22 25 22 28
Peroxisomes – 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 1 3 3 17 2 10 1 13 0 0 2 2 4 6 18 10 22 11 18 23
Vac/Vac Membrane – 32 12 48 39 41 67 83 40 54 41 49 50 100 99 179 151 161 1 4 4 10 41 52
Unique Cell Count 60 39 160 267 335 381 579 320 415 409 493 110 217 256 292 302 295 56 96 90 99 204 255
Labelled Cell Count 72 45 193 310 427 508 719 430 561 523 678 129 253 282 373 387 370 56 96 90 99 204 255


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 14.2 9.5 12.4 9.3 6.9 5.9 6.0 6.1 5.4 5.7 5.6 13.9 14.7 13.1 13.8 14.1 15.3 15.0 15.4 15.4
Std Deviation (1e-4) – 4.6 3.2 3.7 2.9 2.1 1.7 1.8 1.8 1.7 1.7 1.9 4.2 4.1 4.6 3.2 3.4 3.8 4.7 5.0 4.7
Intensity Change (Log2) – – – 0.38 -0.03 -0.45 -0.69 -0.67 -0.63 -0.82 -0.74 -0.75 0.56 0.63 0.47 0.54 0.57 0.69 0.66 0.7 0.7


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0.9 2.0 2.1 0 1.1 1.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -0.8 0 0 0 0 0 0 0 0 -2.4 -1.7 0 -1.2 -2.4 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.0 5.8 4.6 5.0 5.9 5.2 6.0 0 0 0 0 0 0
Nucleus 0 6.0 5.1 3.0 3.1 4.3 3.2 4.0 3.5 2.3 0 2.9 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.8 -0.7 -2.3 -2.0 -1.8 -1.9 -2.6 -2.3 -2.3 -2.4 -2.7 0 -2.1 -1.7 -2.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.1 -2.5 -3.1 -2.0 -2.8 -3.1 -3.0 0 0 1.6 1.8 0.9 3.6 2.3 2.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0084 0.0011 0.0103 0.0001 0.0001 0.0004 0.0151 0.0001 0.0002 0.0005 0.0008 0.0001 – – – – – –
Bud 0.001 0.0022 0.0013 0.0014 0.0015 0.0009 0.0006 0.001 0.0011 0.0022 0.0015 0.0006 – – – – – –
Bud Neck 0.0093 0.0168 0.0108 0.0109 0.0175 0.0131 0.0081 0.0047 0.0096 0.0302 0.0077 0.0114 – – – – – –
Bud Periphery 0.0011 0.0009 0.0014 0.0026 0.0018 0.001 0.0009 0.0007 0.0009 0.0019 0.0032 0.001 – – – – – –
Bud Site 0.0116 0.0186 0.016 0.0082 0.009 0.0046 0.0049 0.0229 0.0205 0.0657 0.0064 0.004 – – – – – –
Cell Periphery 0.0006 0.0009 0.0008 0.0022 0.0014 0.0005 0.0005 0.0009 0.0011 0.0016 0.0009 0.0004 – – – – – –
Cytoplasm 0.0574 0.0253 0.0251 0.0052 0.0077 0.0197 0.0725 0.0917 0.0144 0.0194 0.0012 0.0028 – – – – – –
Cytoplasmic Foci 0.0491 0.0277 0.0184 0.0019 0.0211 0.0231 0.0112 0.027 0.045 0.0257 0.0048 0.0295 – – – – – –
Eisosomes 0.0011 0 0.0001 0 0 0 0.0004 0 0 0 0 0 – – – – – –
Endoplasmic Reticulum 0.0118 0.0019 0.0016 0.0005 0.0004 0.0012 0.0067 0.0021 0.0012 0.0013 0.0007 0.0003 – – – – – –
Endosome 0.0759 0.0123 0.0304 0.0059 0.0124 0.0396 0.051 0.0033 0.0266 0.0291 0.0041 0.0067 – – – – – –
Golgi 0.0069 0.0037 0.007 0.0004 0.0016 0.0045 0.004 0.0005 0.0025 0.0025 0.0018 0.0022 – – – – – –
Lipid Particles 0.017 0.0035 0.0138 0.0011 0.0072 0.0098 0.0178 0.0028 0.0227 0.0271 0.0089 0.015 – – – – – –
Mitochondria 0.0027 0.0074 0.0045 0.0003 0.0009 0.0038 0.0044 0.0007 0.0004 0.001 0.0011 0.0009 – – – – – –
None 0.0647 0.016 0.0017 0.0002 0.0004 0.001 0.0106 0.0309 0.0091 0.0012 0.0003 0.0005 – – – – – –
Nuclear Periphery 0.048 0.0034 0.0055 0.013 0.0083 0.0089 0.0644 0.0065 0.008 0.0101 0.0966 0.0031 – – – – – –
Nucleolus 0.1736 0.3719 0.3988 0.3541 0.4792 0.4077 0.2168 0.267 0.4612 0.3784 0.3654 0.4377 – – – – – –
Nucleus 0.3525 0.4342 0.4165 0.535 0.294 0.3562 0.4514 0.5128 0.3069 0.3535 0.3773 0.3943 – – – – – –
Peroxisomes 0.0017 0.0008 0.0023 0.0002 0.0005 0.001 0.0032 0.0024 0.0012 0.0031 0.0007 0.0079 – – – – – –
Punctate Nuclear 0.0811 0.0339 0.0189 0.0171 0.1165 0.0696 0.0244 0.0038 0.0254 0.0223 0.1052 0.0769 – – – – – –
Vacuole 0.0211 0.0164 0.0139 0.0383 0.0173 0.0305 0.0259 0.0172 0.0408 0.022 0.0052 0.0046 – – – – – –
Vacuole Periphery 0.0035 0.0011 0.0009 0.0013 0.001 0.0031 0.0054 0.001 0.0014 0.0011 0.0061 0.0004 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 31.3164 27.3993 40.663 28.3146 63.9283 34.6288 29.5102 27.234 36.4946 30.394
Translational Efficiency 3.1018 3.652 1.8988 3.0356 1.4187 1.5623 1.9528 1.7492 1.0802 1.7742

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Methyltransferase that methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern; functional homolog of human WBSCR22
Localization
Cell Percentages nucleus (33%), nucleolus (16%), mixed (39%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Bud23

Bud23


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bud23-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available