Standard name
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0.05 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.05 0 0 0 0 – 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.68 0.84 0.9 0.89 0.85 0.8 0.84 – 0.8 0.78 0.67 0.78 0.96 0.86 0.81 0.11 0.15 0.12 0.19 0.27 0.24 0.42 0.5 0.43
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Endosome 0 0.07 0 0 0.05 0.08 0.06 – 0 0 0 0 0 0 0.11 0.12 0.14 0.17 0.24 0.21 0.1 0.06 0 0.08
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.4 0.36 0.37 0 0 0 0 0 0
Mitochondria 0.22 0.05 0 0 0 0.05 0.07 – 0.18 0.09 0.4 0.15 0 0 0 0.1 0.09 0.13 0.2 0.24 0.3 0.21 0.18 0.17
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.06 0.05 0 – 0 0.06 0 0 0 0.08 0.06 0.06 0.07 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0 0.07 0 0 0 0 – 0 0.06 0 0.05 0 0 0 0.53 0.46 0.45 0.26 0.16 0.24 0.13 0.15 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 2 2 5 4 3 5 – 0 2 0 0 0 6 1 15 7 2 3 4 4 1 3 9
Bud 3 2 1 3 1 4 7 – 6 6 7 2 0 1 1 2 0 3 0 0 1 0 2 6
Bud Neck 1 0 4 2 3 5 11 – 6 13 4 6 0 2 2 0 0 0 0 2 0 0 0 1
Bud Site 0 0 0 0 0 0 0 – 0 0 2 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 1 1 0 1 – 2 1 0 1 1 1 0 1 2 0 0 0 0 0 0 0
Cytoplasm 117 112 78 87 183 180 296 – 177 221 131 161 23 205 209 39 43 26 41 43 29 22 121 139
Endoplasmic Reticulum 3 3 0 0 1 3 0 – 0 1 3 2 1 4 4 17 8 12 1 0 1 0 2 3
Endosome 6 9 1 3 10 19 20 – 5 11 0 3 0 4 28 44 40 37 53 33 12 3 10 25
Golgi 0 0 0 0 0 1 0 – 0 2 0 0 0 2 3 141 102 81 5 3 1 0 0 2
Mitochondria 38 7 1 0 1 11 24 – 40 26 78 32 0 0 3 37 25 28 44 39 36 11 44 55
Nucleus 0 1 0 0 0 0 0 – 2 2 2 2 0 0 2 1 3 1 0 0 0 0 1 2
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 1 1 0 1 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 2 0 0 0 1 5 8 1 0 0 0 0 0 0
Peroxisomes 0 1 0 1 0 1 5 – 0 1 0 0 0 1 8 0 1 3 5 2 1 0 0 1
SpindlePole 3 2 0 4 13 11 15 – 8 18 8 9 0 18 15 22 20 9 4 2 0 1 9 9
Vac/Vac Membrane 9 4 6 1 7 6 11 – 9 17 4 10 0 2 5 187 128 99 57 26 30 7 35 61
Unique Cell Count 173 134 87 98 216 224 353 222 283 196 207 24 239 259 356 280 219 222 161 123 55 241 327
Labelled Cell Count 182 144 93 107 224 244 395 255 321 241 229 25 246 282 512 388 302 222 161 123 55 241 327


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 4.9 5.4 5.2 5.8 4.6 4.7 – 3.9 4.3 3.4 3.8 5.1 5.5 5.6 12.2 12.5 11.3 4.8 5.2 5.5
Std Deviation (1e-4) 0.5 1.0 1.6 1.8 1.7 1.2 1.5 – 0.8 1.5 0.8 0.8 0.7 1.1 1.6 2.5 2.5 2.5 1.0 1.4 1.3
Intensity Change (Log2) – – – -0.05 0.1 -0.25 -0.22 – -0.49 -0.33 -0.68 -0.5 -0.09 0.03 0.04 1.17 1.21 1.06 -0.18 -0.05 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.2 -1.1 -2.0 -1.4 – -2.1 -2.4 -4.0 -2.4 0.9 -0.9 -1.9 -14.9 -12.9 -13.0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 2.8 3.1 3.4 3.8
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 7.1 6.6 6.6
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 2.8 2.5 3.1
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 -0.3 0 0 0 0 0 7.7 6.6 6.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.8894 -0.6689 -0.7384 -1.002 -0.8039 -0.7442 0.3934 0.9824 0.5965 0.464 0.4567 0.6265 -0.4398 -0.6105 -0.571 0.4214 -0.0645 -0.585
Actin 0.0193 0.0044 0.0041 0.0355 0.0006 0.0273 0.0061 0.0125 0.0095 0.0011 0.0105 0.0063 0.0227 0.0096 0.0128 0.011 0.0604 0.007
Bud 0.0007 0.0014 0.0113 0.0022 0.0004 0.0041 0.0004 0.0022 0.0009 0.0017 0.0005 0.0005 0.0007 0.0004 0.0007 0.0018 0.0004 0.0001
Bud Neck 0.001 0.0006 0.005 0.0007 0.0266 0.0091 0.0007 0.0008 0.0006 0.0008 0.0005 0.0042 0.0013 0.0003 0.0075 0.0008 0.0008 0.001
Bud Periphery 0.002 0.0045 0.0027 0.0031 0.0009 0.0037 0.0007 0.0037 0.0024 0.0024 0.0007 0.001 0.0008 0.0003 0.0005 0.0023 0.0004 0.0002
Bud Site 0.004 0.0054 0.0071 0.0035 0.0819 0.0021 0.0018 0.009 0.0016 0.0028 0.0016 0.0004 0.0022 0.0025 0.0032 0.009 0.0011 0.0002
Cell Periphery 0.0006 0.0006 0.0005 0.0005 0.0021 0.0005 0.0002 0.0003 0.0002 0.0001 0.0001 0.0001 0.0002 0.0002 0.0003 0.0003 0.0001 0.0001
Cytoplasm 0.0441 0.0518 0.0534 0.1131 0.0549 0.056 0.0609 0.0805 0.0578 0.0355 0.0276 0.0705 0.0605 0.1358 0.0696 0.0348 0.0765 0.0968
Cytoplasmic Foci 0.1616 0.177 0.171 0.246 0.0552 0.1621 0.2339 0.2204 0.2434 0.2638 0.2365 0.2132 0.2063 0.2165 0.2147 0.141 0.2321 0.2168
Eisosomes 0.0008 0.0022 0.0004 0.0004 0.0005 0.0004 0.0002 0.0002 0.0002 0 0.0002 0.0001 0.0011 0.0001 0.0003 0.0003 0.0001 0.0001
Endoplasmic Reticulum 0.0036 0.003 0.007 0.0067 0.0078 0.0027 0.002 0.0021 0.0022 0.0009 0.0018 0.0014 0.0058 0.0031 0.0028 0.0012 0.0028 0.0031
Endosome 0.1247 0.1648 0.197 0.2517 0.2562 0.093 0.2018 0.23 0.2158 0.2831 0.2064 0.1729 0.2248 0.2406 0.2124 0.3375 0.2528 0.2259
Golgi 0.0436 0.0209 0.0444 0.0452 0.0051 0.0315 0.0545 0.0513 0.0505 0.0472 0.063 0.0583 0.0359 0.0353 0.0346 0.0732 0.0562 0.0481
Lipid Particles 0.0935 0.0481 0.0564 0.0634 0.09 0.0439 0.0534 0.0347 0.0262 0.0363 0.0669 0.0285 0.0771 0.0403 0.0282 0.0729 0.081 0.0406
Mitochondria 0.0515 0.0445 0.0249 0.0227 0.0189 0.0351 0.021 0.0391 0.0319 0.0331 0.0292 0.0278 0.0164 0.0119 0.0102 0.0121 0.0042 0.007
None 0.3659 0.4211 0.316 0.1707 0.3428 0.444 0.2322 0.2068 0.2182 0.1601 0.1913 0.2493 0.23 0.2537 0.3389 0.2506 0.1091 0.2743
Nuclear Periphery 0.0062 0.0014 0.0023 0.0019 0.0075 0.0006 0.0011 0.0019 0.0013 0.0006 0.0007 0.0026 0.0142 0.0015 0.0026 0.0018 0.003 0.0038
Nucleolus 0.0022 0.0008 0.0014 0.0004 0.0066 0.0006 0.0018 0.0018 0.0008 0.0004 0.0003 0.0005 0.0017 0.0004 0.0015 0.0101 0.0009 0.0003
Nucleus 0.0047 0.0009 0.0014 0.0011 0.0034 0.0006 0.0028 0.0035 0.0014 0.0008 0.0004 0.0014 0.0039 0.0012 0.0015 0.0027 0.0014 0.0009
Peroxisomes 0.0458 0.035 0.0589 0.0144 0.01 0.071 0.1126 0.0802 0.122 0.1154 0.1545 0.1498 0.0671 0.0318 0.041 0.0241 0.062 0.0637
Punctate Nuclear 0.0071 0.0017 0.0159 0.0039 0.0025 0.0033 0.004 0.0058 0.0048 0.0017 0.0022 0.006 0.0175 0.0017 0.008 0.0049 0.0496 0.0031
Vacuole 0.0127 0.0083 0.0157 0.0096 0.02 0.0057 0.0063 0.0104 0.0061 0.0099 0.0035 0.0037 0.0068 0.01 0.0064 0.0055 0.0035 0.0049
Vacuole Periphery 0.0045 0.0017 0.0031 0.0032 0.0059 0.0025 0.0016 0.0027 0.0021 0.0022 0.0014 0.0016 0.0031 0.0026 0.0021 0.0021 0.0016 0.0019

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 36.6638 33.8156 29.7252 29.8544 26.0317 57.0009 55.4946 56.8039 49.9437 39.8509
Translational Efficiency 1.1281 1.0839 1.215 1.2816 1.0122 1.0381 1.0826 1.0897 1.4139 0.9949

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✔
Standard name
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Tvs1

Tvs1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tvs1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available