Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.27
Bud 0 0
Bud Neck 0.09 0
Bud Site
Cell Periphery 0 0
Cytoplasm 0 0
Endoplasmic Reticulum 0 0
Endosome 0 0.06
Golgi 0 0
Mitochondria 0 0.62
Nucleus 0 0
Nuclear Periphery 0 0
Nucleolus 0.49 0
Peroxisomes 0 0
SpindlePole 0 0
Vac/Vac Membrane 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0
Bud 0 0
Bud Neck 0 0
Bud Site
Cell Periphery 0 0
Cytoplasm 0 0
Endoplasmic Reticulum 0 0
Endosome 0 0
Golgi 0 0
Mitochondria 0 0
Nucleus 0 0
Nuclear Periphery 0 0
Nucleolus 0 0
Peroxisomes 0 0
SpindlePole 0 0
Vac/Vac Membrane 0 0
Unique Cell Count 1 1
Labelled Cell Count 1 1


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4)
Std Deviation (1e-4)
Intensity Change (Log2)


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.0583 9.3854 9.0362 6.4372 4.53 7.264 4.0457 11.8891 10.5974 9.7588 25.8047 8.2484 0.3523 0.5648 3.3962 1.1729 7.246 3.0269
Actin 0.0667 0.0134 0.0037 0.0479 0.0106 0.0211 0.0636 0.0014 0.0021 0.0359 0.0464 0.004 0.0018 0.0044 0.0175 0.0335 0.0007 0.001
Bud 0.0102 0.0165 0.0118 0.0009 0.0167 0.0015 0.0024 0.013 0.0101 0.0014 0.0086 0.0013 0.0019 0.0378 0.0022 0.0004 0.0001 0.0009
Bud Neck 0.0063 0.001 0.0003 0.0014 0.0017 0.0024 0.0013 0.0005 0.0004 0.001 0.0019 0.0015 0.0005 0.0009 0.0008 0.0025 0.0008 0.0008
Bud Periphery 0.0166 0.0076 0.0063 0.0013 0.0263 0.002 0.0017 0.0053 0.0045 0.0013 0.0058 0.0011 0.0012 0.0123 0.0018 0.0006 0 0.001
Bud Site 0.034 0.053 0.0032 0.003 0.0098 0.0003 0.0055 0.0049 0.0029 0.0043 0.0025 0.0003 0.0009 0.0154 0.0147 0.0045 0.0018 0.0001
Cell Periphery 0.0027 0.0015 0.0007 0.0004 0.005 0.0003 0.0007 0.0005 0.0001 0.0002 0.0007 0.0002 0.0005 0.0004 0.0005 0.0001 0 0.0001
Cytoplasm 0.322 0.5369 0.6112 0.5514 0.5759 0.6677 0.4152 0.6552 0.7106 0.6512 0.7611 0.6831 0.3296 0.371 0.6406 0.6125 0.4958 0.5177
Cytoplasmic Foci 0.0206 0.0044 0.0055 0.0123 0.0076 0.0179 0.0473 0.0073 0.0073 0.0178 0.0144 0.0137 0.0866 0.0563 0.0044 0.0311 0.0711 0.0114
Eisosomes 0.0015 0.0007 0.0008 0.001 0.0011 0.0001 0.001 0.0002 0 0.0001 0.0002 0.0001 0.0001 0.0001 0.0002 0.0002 0.0001 0
Endoplasmic Reticulum 0.0119 0.0025 0.0053 0.0028 0.0074 0.0045 0.0091 0.0013 0.0017 0.0018 0.0018 0.0038 0.0044 0.0018 0.0022 0.0005 0.0013 0.0019
Endosome 0.0357 0.0023 0.0079 0.0127 0.0164 0.0047 0.0335 0.0028 0.0112 0.051 0.0078 0.0088 0.0042 0.0008 0.0039 0.0026 0.0006 0.0008
Golgi 0.0179 0.0005 0.0007 0.0263 0.0087 0.0025 0.013 0.0026 0.0137 0.0148 0.0078 0.0027 0.0019 0.0008 0.0061 0.0067 0.0008 0.0001
Lipid Particles 0.0149 0.0015 0.0007 0.0047 0.0029 0.0009 0.0242 0.0006 0.0075 0.0322 0.0197 0.0032 0.0052 0.0002 0.0021 0.0168 0.0056 0.0003
Mitochondria 0.0273 0.0066 0.0387 0.0524 0.0547 0.0027 0.0423 0.0006 0.0172 0.013 0.0029 0.0125 0.0122 0.0004 0.0243 0.0089 0.0015 0.0001
None 0.3732 0.3374 0.2916 0.2688 0.2191 0.2592 0.2477 0.2992 0.2038 0.1366 0.0911 0.2516 0.4828 0.4896 0.2734 0.2333 0.3507 0.4595
Nuclear Periphery 0.0062 0.0007 0.0018 0.0009 0.0027 0.0013 0.0149 0.0004 0.001 0.0012 0.0024 0.0045 0.0016 0.0005 0.0005 0.0002 0.0004 0.0006
Nucleolus 0.0011 0.0004 0.0003 0.0001 0.0004 0.0002 0.0027 0.0004 0.0001 0.0029 0.0003 0.0002 0.003 0.0001 0.0001 0.0001 0.0003 0
Nucleus 0.0029 0.004 0.0034 0.0027 0.0065 0.0042 0.0213 0.0013 0.0014 0.0023 0.0033 0.0028 0.0072 0.0023 0.0013 0.0008 0.0026 0.0016
Peroxisomes 0.0112 0.0036 0.0007 0.0061 0.0025 0.001 0.0248 0.0003 0.0025 0.0144 0.0142 0.0004 0.0434 0.0041 0.0021 0.0427 0.0639 0.0007
Punctate Nuclear 0.0055 0.0009 0.0006 0.0004 0.0003 0.0024 0.0162 0.0012 0.0007 0.0042 0.0052 0.0017 0.0032 0.0004 0.0005 0.0014 0.0016 0.0004
Vacuole 0.0084 0.0044 0.0041 0.0018 0.0202 0.0027 0.0085 0.0009 0.0007 0.0092 0.0016 0.0015 0.0057 0.0004 0.0006 0.0002 0.0002 0.0007
Vacuole Periphery 0.0032 0.0002 0.0007 0.0007 0.0036 0.0006 0.0029 0.0001 0.0004 0.0032 0.0004 0.0011 0.0022 0.0001 0.0002 0.0001 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.5904 6.4469 12.9848 14.7169 8.1816 12.0988 13.5025 14.0627 12.4258 14.1179
Translational Efficiency 0.3418 0.5559 0.3322 0.2742 0.3659 0.2906 0.3349 0.2288 0.3045 0.2529

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression
Localization
Cell Percentages cytoplasm (73%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Srb8

Srb8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Srb8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available