Standard name
Human Ortholog
Description Mitochondrial thioredoxin; highly conserved oxidoreductase required to maintain the redox homeostasis of the cell, forms the mitochondrial thioredoxin system with Trr2p, redox state is maintained by both Trr2p and Glr1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0 0 0 0 0 0 0.05
Bud 0 0 0.06 0.05 0.06 0 0 0.06 0 0 0.06 0 0 0 0 0.05 0.09 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0.1 0 0 0 0 0 0
Cytoplasm 0.07 0.25 0.18 0.06 0.07 0.05 0 0 0 0 0 0 0.16 0.21 0.27 0.11 0.18 0.08 0 0.05 0 0 0 0
Endoplasmic Reticulum 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0
Endosome 0 0.11 0 0 0.24 0 0 0 0 0 0 0 0.09 0.15 0.2 0.07 0.05 0.12 0.08 0.07 0 0.08 0.1 0.09
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.4 0.43 0.35 0 0 0 0 0.05 0
Mitochondria 0.94 0.59 0.82 0.93 0.73 0.9 0.89 0.84 0.94 0.91 0.95 0.89 0.69 0.6 0.48 0.05 0 0.07 0.66 0.69 0.7 0.7 0.66 0.6
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0.09 0.06 0.07 0.07 0.06 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0.07 0.08 0.05 0.11 0.07 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.05 0.14 0 0 0 0 0 0 0 0.05 0.1 0.16 0.12 0 0.05 0 0 0 0 0 0.06
SpindlePole 0 0 0 0 0.07 0 0 0.05 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.1 0.11 0 0 0.05 0 0 0.05 0 0 0 0 0.11 0.09 0.07 0.12 0.14 0.17 0.08 0 0.07 0.06 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 0 0 0 0 0 0 0 0 0 3 0 0 1 5 0 2 5 0 2 8 20
Bud 1 6 3 6 12 9 10 26 12 17 13 9 8 16 4 3 4 1 0 1 0 0 4 9
Bud Neck 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 2 8
Bud Site 1 0 1 0 0 1 3 7 9 7 1 9 1 0 0 1 0 0 – – – – – –
Cell Periphery 0 11 1 2 2 3 0 1 2 3 2 2 5 11 13 5 1 4 0 1 0 0 0 0
Cytoplasm 7 47 9 7 14 12 13 9 9 9 8 9 49 80 93 6 8 3 2 10 2 2 4 4
Endoplasmic Reticulum 5 2 1 1 1 3 3 1 0 1 1 1 11 8 4 2 3 2 1 3 2 1 2 3
Endosome 0 21 0 0 45 3 5 11 1 3 0 3 27 58 70 4 2 5 9 15 2 8 31 37
Golgi 0 1 0 0 1 0 1 0 0 0 0 0 2 4 5 23 19 14 3 4 1 3 15 16
Mitochondria 96 112 41 115 139 211 329 381 357 363 207 211 205 232 169 3 1 3 77 163 50 73 200 243
Nucleus 2 0 1 1 1 1 6 13 6 4 2 2 1 0 2 3 2 0 0 1 2 0 0 0
Nuclear Periphery 1 0 0 0 1 7 32 26 28 29 13 23 0 0 0 0 0 0 1 2 0 0 2 1
Nucleolus 0 0 1 2 0 6 25 37 20 42 15 22 1 1 4 2 1 1 0 0 0 0 1 0
Peroxisomes 0 5 0 6 26 3 1 11 2 2 0 3 14 40 56 7 0 2 1 4 3 2 10 26
SpindlePole 0 4 2 3 14 9 11 21 7 16 8 4 7 8 9 3 0 1 3 5 0 1 3 4
Vac/Vac Membrane 10 21 0 2 9 9 16 22 16 10 5 3 34 36 25 7 6 7 9 10 4 6 12 22
Unique Cell Count 102 189 50 124 191 234 368 451 380 397 219 236 297 384 350 57 44 40 118 236 72 105 302 406
Labelled Cell Count 123 231 61 145 265 278 455 566 470 506 275 301 368 494 454 70 52 43 118 236 72 105 302 406


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 5.0 4.6 4.8 5.4 4.7 4.6 5.1 4.3 4.8 4.6 4.6 5.0 5.5 5.4 8.5 8.2 8.4 5.4 5.7 6.0
Std Deviation (1e-4) 0.6 1.4 2.6 3.2 2.9 2.3 2.0 2.0 1.8 1.7 2.0 1.9 1.0 1.7 1.6 2.2 1.9 2.3 3.4 2.0 3.2
Intensity Change (Log2) – – – 0.04 0.21 0.01 -0.01 0.14 -0.1 0.05 -0.02 -0.01 0.12 0.25 0.21 0.88 0.82 0.85 0.21 0.29 0.37


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – 0 – – 0 – – – – 0 0 0 0 0 0
Bud Neck – 0 – – 0 – – – – 0 0 0 0 0 0
Bud Site – 0 – – 0 – – – – 0 0 0 0 0 0
Cell Periphery – 0 – – 0 – – – – 0 0 0 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – 0 – – 0 – – – – 0 0 0 0 0 0
Endosome – 3.8 – – 0 – – – – 0 3.0 3.5 0 0 0
Golgi – 0 – – 0 – – – – 0 0 0 5.1 5.2 4.6
Mitochondria – -1.3 – – 0.5 – – – – -1.9 -3.0 -4.5 -8.0 -7.8 -7.0
Nuclear Periphery – 0 – – 0 – – – – 0 0 0 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – 0 – – 0 – – – – 0 0 0 0 0 0
Peroxisomes – 2.8 – – 0 – – – – 0 0 3.0 0 0 0
SpindlePole – 0 – – 0 – – – – 0 0 0 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – 0 – – 0 – – – – 0 0 0 0 0 0
Cytoplasm – 0 – – 0 – – – – -0.3 0.5 1.3 -1.1 0 -1.5
Nucleus – 0 – – 0 – – – – 0 0 0 0 0 0
Vacuole – 0 – – 0 – – – – 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9131 1.5407 1.3071 0.5021 1.0067 1.2239 1.3549 1.4205 1.3755 1.0181 1.2662 1.2559 2.7749 3.1992 2.7479 2.641 2.9336 3.1615
Actin 0.0177 0.0159 0.0155 0.001 0.0013 0.0656 0.0147 0.0156 0.0148 0.0044 0.0157 0.004 0.0253 0.0162 0.0151 0.0096 0.0019 0.0173
Bud 0.002 0.0048 0.0035 0.0005 0.0023 0.0004 0.0006 0.0054 0.0008 0.0004 0.0023 0.0003 0.0005 0.0014 0.0008 0.0016 0.0004 0.0003
Bud Neck 0.002 0.0015 0.0065 0.0001 0.0006 0.0035 0.0006 0.0011 0.0025 0.0001 0.0004 0.0006 0.0007 0.001 0.0018 0.0011 0.0002 0.0037
Bud Periphery 0.0052 0.0062 0.0077 0.0017 0.0246 0.0008 0.0009 0.0058 0.0016 0.0021 0.0065 0.0008 0.0014 0.0023 0.0022 0.0055 0.0004 0.0005
Bud Site 0.0068 0.0443 0.0309 0.0004 0.0008 0.0006 0.0018 0.0376 0.0122 0.0003 0.0013 0.0003 0.0021 0.0169 0.0093 0.0055 0.0008 0.0012
Cell Periphery 0.0016 0.0015 0.0015 0.0004 0.0005 0.0003 0.0003 0.0019 0.0007 0.0007 0.0004 0.0002 0.001 0.0009 0.0079 0.0018 0.0001 0.0002
Cytoplasm 0.0048 0.0141 0.0079 0.0006 0.0004 0.0005 0.0082 0.0239 0.0005 0.0001 0.0091 0.0005 0.0087 0.0097 0.0069 0.0134 0.0011 0.0051
Cytoplasmic Foci 0.046 0.04 0.0506 0.0054 0.0125 0.0127 0.0339 0.0426 0.0085 0.006 0.026 0.0201 0.0232 0.0322 0.022 0.0223 0.0853 0.0199
Eisosomes 0.0005 0.0004 0.0006 0.0003 0.0001 0.0006 0.0003 0.0006 0.002 0.0001 0.0004 0.0001 0.0006 0.0006 0.0023 0.0003 0.0001 0.0003
Endoplasmic Reticulum 0.0088 0.0037 0.0012 0.0004 0.0001 0.0005 0.0043 0.0068 0.0003 0.0001 0.0016 0.0003 0.0081 0.0016 0.0022 0.0005 0.0021 0.0014
Endosome 0.1045 0.0538 0.0641 0.0312 0.0227 0.0323 0.1165 0.0786 0.0385 0.0036 0.0609 0.043 0.0638 0.0855 0.0328 0.0358 0.0934 0.052
Golgi 0.0584 0.0666 0.0458 0.0469 0.0287 0.0436 0.0931 0.0616 0.0397 0.0111 0.0832 0.0614 0.0272 0.0445 0.0422 0.0297 0.1015 0.0654
Lipid Particles 0.0614 0.0158 0.0078 0.0007 0.0196 0.0233 0.0533 0.0077 0.0054 0.0007 0.0426 0.0037 0.0731 0.009 0.0209 0.0057 0.014 0.0043
Mitochondria 0.5456 0.6517 0.7034 0.902 0.8444 0.7962 0.6074 0.6329 0.8521 0.9323 0.6767 0.8418 0.6595 0.7139 0.7805 0.8084 0.63 0.7438
None 0.0046 0.0101 0.014 0.0002 0.0003 0.0003 0.0034 0.009 0.0002 0.0001 0.0031 0.0001 0.0223 0.0029 0.0108 0.0026 0.0003 0.0008
Nuclear Periphery 0.0081 0.0011 0.0003 0.0001 0 0.0007 0.0087 0.001 0.0001 0 0.0236 0.001 0.0132 0.0006 0.0005 0.0006 0.0017 0.0099
Nucleolus 0.0017 0.0005 0.0011 0 0.0002 0.0001 0.0011 0.0016 0.0002 0.0001 0.0006 0.0002 0.0013 0.0003 0.0003 0.0004 0.0001 0.0002
Nucleus 0.0023 0.0006 0.0007 0.0001 0.0002 0.0002 0.0031 0.0057 0.0001 0.0001 0.0019 0.0004 0.0021 0.0005 0.0003 0.0004 0.0003 0.0088
Peroxisomes 0.0729 0.0469 0.0276 0.0043 0.037 0.0126 0.0345 0.0474 0.0163 0.0334 0.0274 0.0173 0.0434 0.0419 0.0355 0.0276 0.0525 0.058
Punctate Nuclear 0.0034 0.0004 0.0003 0 0 0.0003 0.0034 0.0007 0.0001 0 0.0018 0.0003 0.008 0.0002 0.0002 0.0222 0.0002 0.0004
Vacuole 0.0268 0.0128 0.0063 0.0011 0.0018 0.0023 0.0047 0.0095 0.0017 0.0025 0.0075 0.0017 0.009 0.0119 0.0033 0.0032 0.0061 0.0022
Vacuole Periphery 0.0148 0.0073 0.0027 0.0024 0.0017 0.0027 0.0055 0.003 0.0019 0.0018 0.0073 0.002 0.0053 0.006 0.0023 0.0018 0.0074 0.0043

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 43.1017 48.8051 36.2155 44.6606 26.0789 54.5653 33.7636 40.3175 38.8882 42.7747
Translational Efficiency 1.8996 1.2614 1.7861 1.9138 2.401 1.8503 1.974 1.639 1.9962 1.7476

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mitochondrial thioredoxin; highly conserved oxidoreductase required to maintain the redox homeostasis of the cell, forms the mitochondrial thioredoxin system with Trr2p, redox state is maintained by both Trr2p and Glr1p
Localization
Cell Percentages mitochondrion (85%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Trx3

Trx3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Trx3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available