Standard name
Human Ortholog
Description General repressor of transcription; forms complex with Cyc8p, involved in the establishment of repressive chromatin structure through interactions with histones H3 and H4, appears to enhance expression of some genes

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0 0 0 0 0.05 0 0 0 0 0 0.06 0.05 0.09 0 0 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0 0 0 0 0 0 0.06 0.12 0.05 0 0 0 0.1 0 0.1 0 0 0 0 0 0
Nucleus 0.71 0.7 0.76 0.8 0.72 0.6 0.6 0.56 0.52 0.48 0.48 0.43 0.73 0.66 0.66 0.71 0.71 0.69 0.52 0.6 0.66 0.41 0.35 0.42
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.69 0.5 0.44 0.37 0.32 0.57 0.55 0.57 0.7 0.74 0.68 0.7 0.56 0.49 0.54 0.37 0.41 0.4 0.45 0.3 0.28 0.5 0.55 0.44
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 0 0 1 0 0 1 1 1 0 1 0 1 0 0 0 0 0 2 1 0 1 0
Bud Neck 0 0 3 0 4 1 2 0 4 2 1 4 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 1 0 2 9 3 11 15 8 6 1 0 0 0 0 1
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 7 2 2 5 3 11 7 3 3 2 3 10 9 17 11 8 10 0 0 1 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 3 0 0 0 0 0 2
Endosome 0 1 1 1 2 1 1 1 0 0 0 0 0 1 0 0 0 0 0 0 3 1 1 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Mitochondria 1 7 0 1 2 5 7 5 7 13 22 9 2 0 0 26 9 18 0 1 0 0 1 3
Nucleus 70 90 176 162 188 95 137 135 119 103 90 72 123 117 120 178 169 118 59 90 171 40 53 89
Nuclear Periphery 1 0 0 0 1 0 0 1 0 2 3 0 0 0 0 2 1 0 0 0 0 0 0 1
Nucleolus 68 64 102 76 84 91 126 136 160 159 128 118 94 87 98 93 98 68 51 44 73 48 82 94
Peroxisomes 0 1 2 0 0 0 0 0 0 0 0 0 0 1 0 0 2 1 0 0 0 0 0 0
SpindlePole 0 0 3 0 3 0 0 2 0 0 0 1 0 0 0 1 0 0 1 1 2 0 0 4
Vac/Vac Membrane 0 1 3 0 3 0 0 1 4 0 0 0 1 2 4 3 1 4 0 7 6 4 3 7
Unique Cell Count 99 129 233 203 260 159 228 239 229 216 189 168 169 176 183 249 239 172 113 150 262 98 151 212
Labelled Cell Count 140 171 292 243 293 198 293 292 309 298 254 214 231 218 240 314 289 223 113 150 262 98 151 212


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.1 16.8 14.4 14.0 14.8 11.6 11.2 11.6 10.6 9.0 8.9 8.7 16.7 17.7 16.6 24.2 26.1 19.6 16.0 16.4 17.2
Std Deviation (1e-4) 4.3 7.4 4.3 4.3 4.8 4.0 3.6 3.8 3.5 2.8 3.8 2.9 7.0 8.2 7.0 9.9 10.8 10.1 7.4 6.2 7.6
Intensity Change (Log2) -0.04 0.04 -0.31 -0.37 -0.31 -0.44 -0.68 -0.69 -0.73 0.22 0.3 0.2 0.75 0.86 0.45 0.15 0.19 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 3.4 4.1 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 2.6 0 0 0 0 0 2.9 0 4.1 2.4 0 2.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 3.8 5.3 0 0 0 0 5.1 0 5.1
Nucleus 1.1 -0.8 -3.3 -3.6 -4.4 -5.3 -6.1 -5.9 -6.6 -0.6 -2.0 -2.2 -1.0 -1.2 -1.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -1.3 -2.6 2.6 2.5 2.9 5.7 6.4 4.9 5.3 2.3 1.1 2.0 -1.4 -0.6 -0.9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin
Bud
Bud Neck
Bud Periphery
Bud Site
Cell Periphery
Cytoplasm
Cytoplasmic Foci
Eisosomes
Endoplasmic Reticulum
Endosome
Golgi
Lipid Particles
Mitochondria
None
Nuclear Periphery
Nucleolus
Nucleus
Peroxisomes
Punctate Nuclear
Vacuole
Vacuole Periphery

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 116.6765 138.1479 99.4364 92.3975 102.5205 81.3452 139.5648 111.5592 94.2391 113.573
Translational Efficiency 0.8224 0.8942 0.8977 0.8447 0.8832 1.0062 0.7576 0.827 0.9428 0.8178

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description General repressor of transcription; forms complex with Cyc8p, involved in the establishment of repressive chromatin structure through interactions with histones H3 and H4, appears to enhance expression of some genes
Localization
Cell Percentages nucleus (76%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Tup1

Tup1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tup1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available