ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.07 0.06 0.07 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.11 0.18 0.06 0 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.13 0.11 0.06 0.05 0 0 0 0 0 0 0.09 0 0 0 0 0 0 0 0.05 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0.29 0.11 0.05 0.24 0.33 0.27 0.45 0.4 0.52 0.55 0 0 0 0.05 0.12 0.07 0 0 0 0 0 0
Nucleus 0.32 0.24 0.27 0.11 0.13 0.08 0.14 0.12 0.12 0.1 0.1 0.08 0.19 0.11 0.12 0.13 0.14 0.13 0.2 0.18 0.14 0.07 0.05 0.07
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.78 0.81 0.83 0.9 0.82 0.8 0.72 0.68 0.64 0.58 0.52 0.5 0.81 0.83 0.72 0.85 0.86 0.81 0.64 0.68 0.64 0.73 0.61 0.55
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.12 0.1 0.1 0.28 0.37 0.38 0.38 0.38 0.35 0.43 0.3 0.35 0.16 0.17 0.24 0.14 0.08 0.11 0.09 0.08 0.13 0.12 0.21 0.22
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1 0 1 7 5 20 11 7 19 11 1 1 1 0 0 0 5 0 1 0 1 2
Bud Neck 2 0 0 4 5 5 5 5 3 6 3 3 4 7 6 1 0 0 1 0 2 1 1 2
Bud Site 3 0 6 5 3 10 8 25 18 28 25 29 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0
Cytoplasm 4 3 1 6 15 13 6 26 13 15 8 11 19 25 72 13 7 12 0 0 0 1 0 0
Endoplasmic Reticulum 0 0 1 1 0 0 0 1 0 0 0 0 1 1 0 2 0 1 0 0 0 0 0 0
Endosome 12 0 1 0 45 45 15 36 19 19 14 16 3 6 36 6 5 3 14 1 7 0 9 12
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 0 2 1
Mitochondria 31 5 61 29 16 96 79 208 214 171 210 232 4 3 4 11 27 11 3 1 0 0 1 1
Nucleus 209 32 58 29 45 33 32 89 56 42 41 35 44 27 47 28 30 20 140 25 29 7 10 17
Nuclear Periphery 3 2 1 0 0 1 0 2 1 0 2 2 0 1 0 1 3 1 0 0 0 0 0 2
Nucleolus 509 109 175 231 284 325 171 521 302 248 209 210 189 194 293 178 190 121 441 97 135 80 126 134
Peroxisomes 0 0 0 5 7 7 1 17 5 7 4 6 0 0 3 0 0 1 0 0 0 0 0 0
SpindlePole 77 14 21 71 130 156 89 295 168 182 119 147 38 40 96 29 18 17 63 11 27 13 43 54
Vac/Vac Membrane 5 1 2 0 2 1 0 3 3 2 7 5 0 2 3 3 5 4 13 3 4 2 5 7
Unique Cell Count 654 135 211 257 347 408 237 769 475 424 402 421 234 235 406 210 221 150 688 143 212 110 209 244
Labelled Cell Count 855 166 328 381 553 699 411 1249 813 727 661 707 303 307 562 273 285 191 688 143 212 110 209 244


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.4 8.7 5.8 7.2 6.4 4.7 4.3 4.3 3.7 3.7 3.5 3.4 8.1 8.0 7.4 14.2 13.6 12.9 8.3 9.8 9.6
Std Deviation (1e-4) 1.5 2.0 1.6 2.2 2.2 1.5 1.7 1.4 1.3 1.1 1.0 1.0 2.1 2.0 2.3 5.2 4.4 4.1 2.1 2.4 2.3
Intensity Change (Log2) – – – 0.31 0.15 -0.29 -0.44 -0.42 -0.65 -0.63 -0.71 -0.75 0.5 0.46 0.35 1.3 1.24 1.16 0.51 0.76 0.74


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2.6 0 0 2.3 0 2.3 0 0 3.9 4.6 6.3 3.3 0 3.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 5.2 0 0 0 0 0 0 0 0 0 4.2 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -4.8 -8.1 0 0 0 0 0 0 0 -8.1 -8.3 -10.7 -6.4 -4.3 -5.1
Nucleus -4.5 -4.3 -6.5 -3.7 -5.7 -5.1 -5.7 -5.5 -6.4 -2.2 -4.3 -5.0 -3.6 -3.6 -3.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.2 -0.3 0 0 0 0 0 0 0 -0.6 -0.1 -3.0 0.5 0.9 -0.6
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 4.8 7.1 0 0 0 0 0 0 0 2.0 2.2 4.1 1.2 -0.7 0.4
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.097 3.9146 3.5131 2.6384 2.0946 3.2186 3.021 3.6271 3.034 2.9459 2.0923 3.2052 4.4321 4.9207 4.827 4.6959 4.0078 4.5922
Actin 0.0129 0 0 0.0279 0.0008 0 0.0069 0 0.0084 0.0017 0.0051 0.004 0.0204 0 0.0064 0.0001 0.0008 0.0142
Bud 0.0001 0 0 0.0002 0.0004 0 0.0006 0 0.0004 0.0003 0.0059 0.0001 0.0004 0 0.0001 0 0.0001 0
Bud Neck 0.0008 0.0006 0.0007 0.002 0.0016 0.0007 0.0008 0.0002 0.0016 0.0004 0.0024 0.0005 0.0016 0.0008 0.001 0.0006 0.0008 0.0007
Bud Periphery 0.0001 0.0001 0.0001 0.0003 0.0006 0.0001 0.0015 0 0.0004 0.0004 0.0114 0.0001 0.0013 0 0.0001 0 0.0001 0.0001
Bud Site 0.001 0.0003 0.0006 0.001 0.0061 0.0002 0.0024 0.0002 0.0024 0.0007 0.0067 0.0002 0.0015 0.0003 0.0012 0.0004 0.0004 0.0001
Cell Periphery 0.0002 0.0002 0.0002 0.0002 0.0005 0.0001 0.0008 0.0001 0.0001 0.0002 0.0005 0 0.0006 0.0001 0.0002 0.0001 0 0
Cytoplasm 0.0042 0 0 0.0002 0.0172 0 0.0259 0.0125 0.003 0.0004 0.0015 0.0201 0.0022 0 0.0011 0.0008 0.0002 0
Cytoplasmic Foci 0.0261 0.0009 0.0042 0.0149 0.0342 0.0011 0.0121 0.0001 0.0047 0.005 0.0161 0.0039 0.0054 0.0003 0.0069 0.0101 0.0079 0.0011
Eisosomes 0.0001 0 0 0.0002 0.0001 0 0.0002 0 0.0002 0 0.0001 0 0.0005 0 0.0001 0 0 0.0001
Endoplasmic Reticulum 0.0005 0 0 0.0004 0.0032 0 0.0037 0 0.0011 0.0003 0.0016 0.0001 0.0037 0 0.0003 0.0003 0.0002 0.0001
Endosome 0.0078 0.0001 0.0004 0.034 0.0778 0.0001 0.0266 0 0.0148 0.0387 0.0918 0.0058 0.0109 0.0002 0.0041 0.0012 0.0025 0.0009
Golgi 0.0035 0.0001 0.0004 0.0336 0.0059 0.0002 0.0103 0 0.007 0.0154 0.0165 0.0113 0.0025 0 0.0017 0.0004 0.0016 0.0013
Lipid Particles 0.017 0.003 0.0123 0.0684 0.0224 0.007 0.0233 0.0002 0.0083 0.0107 0.0075 0.0054 0.0091 0.0007 0.0061 0.0072 0.0155 0.0057
Mitochondria 0.0086 0.0001 0.0005 0.0172 0.0027 0.0001 0.0282 0 0.0035 0.0246 0.0191 0.0065 0.0022 0.0001 0.0005 0.0002 0.0003 0.0008
None 0.0018 0 0 0.0004 0.042 0 0.0011 0 0.0095 0.0005 0.0006 0 0.005 0 0.0057 0.0013 0.0003 0.0004
Nuclear Periphery 0.0037 0 0.0001 0.0009 0.0071 0.0001 0.0135 0.0001 0.0027 0.0007 0.0038 0.0001 0.0105 0.0002 0.0008 0.0024 0.0036 0.0012
Nucleolus 0.8188 0.8834 0.8933 0.6973 0.6838 0.8981 0.7313 0.8562 0.8189 0.801 0.7044 0.8543 0.7908 0.8332 0.845 0.8788 0.6863 0.8601
Nucleus 0.0536 0.1044 0.0681 0.0583 0.069 0.0724 0.071 0.1256 0.1061 0.0776 0.0609 0.0806 0.0898 0.1409 0.1005 0.0602 0.0496 0.0642
Peroxisomes 0.0031 0.0001 0.003 0.0316 0.0022 0.0004 0.0035 0 0.0023 0.0023 0.003 0.0031 0.0036 0 0.0027 0.0003 0.0017 0.0016
Punctate Nuclear 0.0349 0.006 0.015 0.0079 0.0119 0.0193 0.0256 0.0044 0.0032 0.0023 0.0245 0.0034 0.0348 0.0223 0.0141 0.0346 0.2278 0.0471
Vacuole 0.0009 0.0007 0.0007 0.0014 0.0079 0.0002 0.006 0.0003 0.0008 0.0103 0.0112 0.0002 0.0026 0.0006 0.0012 0.0005 0.0002 0.0001
Vacuole Periphery 0.0003 0.0001 0.0001 0.0016 0.0023 0 0.0045 0 0.0004 0.0063 0.0056 0.0002 0.0009 0.0002 0.0002 0.0003 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 72.8438 70.4554 83.9664 49.5656 68.1244 42.966 43.7139 45.895 38.0267 43.1715
Translational Efficiency 2.4954 2.6885 1.84 2.4293 2.3303 3.0251 1.8621 1.9575 1.792 2.3011

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene
Localization
Cell Percentages nucleolus (84%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-2

YCR087C-A

YCR087C-A


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ycr087c-a-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available