ORF | |
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Human Ortholog | |
Description | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 1 | 0 | 1 | 7 | 5 | 20 | 11 | 7 | 19 | 11 | 1 | 1 | 1 | 0 | 0 | 0 | 5 | 0 | 1 | 0 | 1 | 2 |
Bud Neck | 2 | 0 | 0 | 4 | 5 | 5 | 5 | 5 | 3 | 6 | 3 | 3 | 4 | 7 | 6 | 1 | 0 | 0 | 1 | 0 | 2 | 1 | 1 | 2 |
Bud Site | 3 | 0 | 6 | 5 | 3 | 10 | 8 | 25 | 18 | 28 | 25 | 29 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 3 | 1 | 6 | 15 | 13 | 6 | 26 | 13 | 15 | 8 | 11 | 19 | 25 | 72 | 13 | 7 | 12 | 0 | 0 | 0 | 1 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 12 | 0 | 1 | 0 | 45 | 45 | 15 | 36 | 19 | 19 | 14 | 16 | 3 | 6 | 36 | 6 | 5 | 3 | 14 | 1 | 7 | 0 | 9 | 12 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 2 | 1 |
Mitochondria | 31 | 5 | 61 | 29 | 16 | 96 | 79 | 208 | 214 | 171 | 210 | 232 | 4 | 3 | 4 | 11 | 27 | 11 | 3 | 1 | 0 | 0 | 1 | 1 |
Nucleus | 209 | 32 | 58 | 29 | 45 | 33 | 32 | 89 | 56 | 42 | 41 | 35 | 44 | 27 | 47 | 28 | 30 | 20 | 140 | 25 | 29 | 7 | 10 | 17 |
Nuclear Periphery | 3 | 2 | 1 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 2 | 2 | 0 | 1 | 0 | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 2 |
Nucleolus | 509 | 109 | 175 | 231 | 284 | 325 | 171 | 521 | 302 | 248 | 209 | 210 | 189 | 194 | 293 | 178 | 190 | 121 | 441 | 97 | 135 | 80 | 126 | 134 |
Peroxisomes | 0 | 0 | 0 | 5 | 7 | 7 | 1 | 17 | 5 | 7 | 4 | 6 | 0 | 0 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 77 | 14 | 21 | 71 | 130 | 156 | 89 | 295 | 168 | 182 | 119 | 147 | 38 | 40 | 96 | 29 | 18 | 17 | 63 | 11 | 27 | 13 | 43 | 54 |
Vac/Vac Membrane | 5 | 1 | 2 | 0 | 2 | 1 | 0 | 3 | 3 | 2 | 7 | 5 | 0 | 2 | 3 | 3 | 5 | 4 | 13 | 3 | 4 | 2 | 5 | 7 |
Unique Cell Count | 654 | 135 | 211 | 257 | 347 | 408 | 237 | 769 | 475 | 424 | 402 | 421 | 234 | 235 | 406 | 210 | 221 | 150 | 688 | 143 | 212 | 110 | 209 | 244 |
Labelled Cell Count | 855 | 166 | 328 | 381 | 553 | 699 | 411 | 1249 | 813 | 727 | 661 | 707 | 303 | 307 | 562 | 273 | 285 | 191 | 688 | 143 | 212 | 110 | 209 | 244 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.4 | 8.7 | 5.8 | 7.2 | 6.4 | 4.7 | 4.3 | 4.3 | 3.7 | 3.7 | 3.5 | 3.4 | 8.1 | 8.0 | 7.4 | 14.2 | 13.6 | 12.9 | 8.3 | 9.8 | 9.6 |
Std Deviation (1e-4) | 1.5 | 2.0 | 1.6 | 2.2 | 2.2 | 1.5 | 1.7 | 1.4 | 1.3 | 1.1 | 1.0 | 1.0 | 2.1 | 2.0 | 2.3 | 5.2 | 4.4 | 4.1 | 2.1 | 2.4 | 2.3 |
Intensity Change (Log2) | – | – | – | 0.31 | 0.15 | -0.29 | -0.44 | -0.42 | -0.65 | -0.63 | -0.71 | -0.75 | 0.5 | 0.46 | 0.35 | 1.3 | 1.24 | 1.16 | 0.51 | 0.76 | 0.74 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 2.6 | 0 | 0 | 2.3 | 0 | 2.3 | 0 | 0 | 3.9 | 4.6 | 6.3 | 3.3 | 0 | 3.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 5.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.2 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -4.8 | -8.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -8.1 | -8.3 | -10.7 | -6.4 | -4.3 | -5.1 |
Nucleus | -4.5 | -4.3 | -6.5 | -3.7 | -5.7 | -5.1 | -5.7 | -5.5 | -6.4 | -2.2 | -4.3 | -5.0 | -3.6 | -3.6 | -3.2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2.2 | -0.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.6 | -0.1 | -3.0 | 0.5 | 0.9 | -0.6 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 4.8 | 7.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.0 | 2.2 | 4.1 | 1.2 | -0.7 | 0.4 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.097 | 3.9146 | 3.5131 | 2.6384 | 2.0946 | 3.2186 | 3.021 | 3.6271 | 3.034 | 2.9459 | 2.0923 | 3.2052 | 4.4321 | 4.9207 | 4.827 | 4.6959 | 4.0078 | 4.5922 |
Actin | 0.0129 | 0 | 0 | 0.0279 | 0.0008 | 0 | 0.0069 | 0 | 0.0084 | 0.0017 | 0.0051 | 0.004 | 0.0204 | 0 | 0.0064 | 0.0001 | 0.0008 | 0.0142 |
Bud | 0.0001 | 0 | 0 | 0.0002 | 0.0004 | 0 | 0.0006 | 0 | 0.0004 | 0.0003 | 0.0059 | 0.0001 | 0.0004 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Bud Neck | 0.0008 | 0.0006 | 0.0007 | 0.002 | 0.0016 | 0.0007 | 0.0008 | 0.0002 | 0.0016 | 0.0004 | 0.0024 | 0.0005 | 0.0016 | 0.0008 | 0.001 | 0.0006 | 0.0008 | 0.0007 |
Bud Periphery | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0006 | 0.0001 | 0.0015 | 0 | 0.0004 | 0.0004 | 0.0114 | 0.0001 | 0.0013 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 |
Bud Site | 0.001 | 0.0003 | 0.0006 | 0.001 | 0.0061 | 0.0002 | 0.0024 | 0.0002 | 0.0024 | 0.0007 | 0.0067 | 0.0002 | 0.0015 | 0.0003 | 0.0012 | 0.0004 | 0.0004 | 0.0001 |
Cell Periphery | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0005 | 0.0001 | 0.0008 | 0.0001 | 0.0001 | 0.0002 | 0.0005 | 0 | 0.0006 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 |
Cytoplasm | 0.0042 | 0 | 0 | 0.0002 | 0.0172 | 0 | 0.0259 | 0.0125 | 0.003 | 0.0004 | 0.0015 | 0.0201 | 0.0022 | 0 | 0.0011 | 0.0008 | 0.0002 | 0 |
Cytoplasmic Foci | 0.0261 | 0.0009 | 0.0042 | 0.0149 | 0.0342 | 0.0011 | 0.0121 | 0.0001 | 0.0047 | 0.005 | 0.0161 | 0.0039 | 0.0054 | 0.0003 | 0.0069 | 0.0101 | 0.0079 | 0.0011 |
Eisosomes | 0.0001 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0005 | 0 | 0.0001 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0005 | 0 | 0 | 0.0004 | 0.0032 | 0 | 0.0037 | 0 | 0.0011 | 0.0003 | 0.0016 | 0.0001 | 0.0037 | 0 | 0.0003 | 0.0003 | 0.0002 | 0.0001 |
Endosome | 0.0078 | 0.0001 | 0.0004 | 0.034 | 0.0778 | 0.0001 | 0.0266 | 0 | 0.0148 | 0.0387 | 0.0918 | 0.0058 | 0.0109 | 0.0002 | 0.0041 | 0.0012 | 0.0025 | 0.0009 |
Golgi | 0.0035 | 0.0001 | 0.0004 | 0.0336 | 0.0059 | 0.0002 | 0.0103 | 0 | 0.007 | 0.0154 | 0.0165 | 0.0113 | 0.0025 | 0 | 0.0017 | 0.0004 | 0.0016 | 0.0013 |
Lipid Particles | 0.017 | 0.003 | 0.0123 | 0.0684 | 0.0224 | 0.007 | 0.0233 | 0.0002 | 0.0083 | 0.0107 | 0.0075 | 0.0054 | 0.0091 | 0.0007 | 0.0061 | 0.0072 | 0.0155 | 0.0057 |
Mitochondria | 0.0086 | 0.0001 | 0.0005 | 0.0172 | 0.0027 | 0.0001 | 0.0282 | 0 | 0.0035 | 0.0246 | 0.0191 | 0.0065 | 0.0022 | 0.0001 | 0.0005 | 0.0002 | 0.0003 | 0.0008 |
None | 0.0018 | 0 | 0 | 0.0004 | 0.042 | 0 | 0.0011 | 0 | 0.0095 | 0.0005 | 0.0006 | 0 | 0.005 | 0 | 0.0057 | 0.0013 | 0.0003 | 0.0004 |
Nuclear Periphery | 0.0037 | 0 | 0.0001 | 0.0009 | 0.0071 | 0.0001 | 0.0135 | 0.0001 | 0.0027 | 0.0007 | 0.0038 | 0.0001 | 0.0105 | 0.0002 | 0.0008 | 0.0024 | 0.0036 | 0.0012 |
Nucleolus | 0.8188 | 0.8834 | 0.8933 | 0.6973 | 0.6838 | 0.8981 | 0.7313 | 0.8562 | 0.8189 | 0.801 | 0.7044 | 0.8543 | 0.7908 | 0.8332 | 0.845 | 0.8788 | 0.6863 | 0.8601 |
Nucleus | 0.0536 | 0.1044 | 0.0681 | 0.0583 | 0.069 | 0.0724 | 0.071 | 0.1256 | 0.1061 | 0.0776 | 0.0609 | 0.0806 | 0.0898 | 0.1409 | 0.1005 | 0.0602 | 0.0496 | 0.0642 |
Peroxisomes | 0.0031 | 0.0001 | 0.003 | 0.0316 | 0.0022 | 0.0004 | 0.0035 | 0 | 0.0023 | 0.0023 | 0.003 | 0.0031 | 0.0036 | 0 | 0.0027 | 0.0003 | 0.0017 | 0.0016 |
Punctate Nuclear | 0.0349 | 0.006 | 0.015 | 0.0079 | 0.0119 | 0.0193 | 0.0256 | 0.0044 | 0.0032 | 0.0023 | 0.0245 | 0.0034 | 0.0348 | 0.0223 | 0.0141 | 0.0346 | 0.2278 | 0.0471 |
Vacuole | 0.0009 | 0.0007 | 0.0007 | 0.0014 | 0.0079 | 0.0002 | 0.006 | 0.0003 | 0.0008 | 0.0103 | 0.0112 | 0.0002 | 0.0026 | 0.0006 | 0.0012 | 0.0005 | 0.0002 | 0.0001 |
Vacuole Periphery | 0.0003 | 0.0001 | 0.0001 | 0.0016 | 0.0023 | 0 | 0.0045 | 0 | 0.0004 | 0.0063 | 0.0056 | 0.0002 | 0.0009 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 72.8438 | 70.4554 | 83.9664 | 49.5656 | 68.1244 | 42.966 | 43.7139 | 45.895 | 38.0267 | 43.1715 |
Translational Efficiency | 2.4954 | 2.6885 | 1.84 | 2.4293 | 2.3303 | 3.0251 | 1.8621 | 1.9575 | 1.792 | 2.3011 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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