Standard name | |
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Human Ortholog | |
Description | Mismatch repair protein; forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 1 | 4 | 5 | 5 | 11 | 8 | 6 | 6 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 5 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 5 | 4 | 11 | 13 | 11 | 17 | 17 | 16 | 7 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 7 | 3 | 4 | 5 | 18 | 22 | 30 | 45 | 24 | 39 | 24 | 30 | 23 | 65 | 92 | 20 | 16 | 36 | 0 | 1 | 0 | 0 | 3 | 3 |
Endoplasmic Reticulum | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 10 | 7 | 8 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 2 | 4 | 3 | 0 | 0 | 0 | 3 | 6 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 1 | 1 | 0 | 1 |
Mitochondria | 9 | 35 | 113 | 161 | 157 | 333 | 439 | 481 | 455 | 525 | 359 | 517 | 10 | 14 | 48 | 21 | 10 | 29 | 1 | 1 | 0 | 1 | 3 | 12 |
Nucleus | 180 | 111 | 182 | 248 | 334 | 360 | 427 | 503 | 335 | 351 | 244 | 262 | 264 | 322 | 344 | 280 | 183 | 253 | 176 | 111 | 184 | 159 | 299 | 218 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 3 | 1 | 4 | 1 | 1 | 1 | 0 | 0 | 4 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 2 | 2 | 0 | 4 | 3 | 4 | 2 | 3 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 11 | 18 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 1 | 1 | 2 | 2 | 5 | 3 | 5 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 5 | 0 | 0 | 0 | 0 | 4 | 2 |
Vac/Vac Membrane | 6 | 9 | 1 | 2 | 10 | 9 | 14 | 18 | 10 | 4 | 8 | 11 | 2 | 8 | 14 | 28 | 25 | 37 | 11 | 9 | 25 | 17 | 67 | 98 |
Unique Cell Count | 195 | 130 | 212 | 287 | 409 | 483 | 643 | 764 | 553 | 624 | 425 | 573 | 292 | 392 | 460 | 323 | 219 | 326 | 200 | 128 | 214 | 185 | 403 | 371 |
Labelled Cell Count | 206 | 158 | 302 | 424 | 529 | 743 | 937 | 1074 | 860 | 955 | 663 | 839 | 301 | 414 | 502 | 366 | 244 | 376 | 200 | 128 | 214 | 185 | 403 | 371 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.8 | 4.3 | 3.6 | 3.5 | 3.7 | 3.3 | 3.2 | 3.2 | 2.8 | 2.8 | 2.8 | 2.5 | 5.2 | 5.2 | 4.5 | 6.3 | 6.7 | 7.0 | 5.3 | 4.7 | 5.4 |
Std Deviation (1e-4) | 1.1 | 0.6 | 0.7 | 0.7 | 0.6 | 0.7 | 0.8 | 0.7 | 0.7 | 0.6 | 0.7 | 0.6 | 1.1 | 1.3 | 1.2 | 1.3 | 1.6 | 1.9 | 1.0 | 1.0 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.05 | 0.03 | -0.14 | -0.18 | -0.16 | -0.4 | -0.4 | -0.4 | -0.53 | 0.52 | 0.52 | 0.3 | 0.8 | 0.89 | 0.95 | 0.53 | 0.37 | 0.59 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 4.1 | 4.8 | 4.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.1291 | 1.1864 | 1.1174 | 1.0663 | 0.5704 | 1.063 | 1.5019 | 1.9036 | 1.6092 | 1.5148 | 1.1378 | 1.3396 | 0.6493 | 0.8256 | 0.6191 | 0.5494 | 0.6708 | 0.3452 |
Actin | 0.0326 | 0.0001 | 0.0241 | 0.0047 | 0.0076 | 0.0135 | 0.0408 | 0.0008 | 0.0022 | 0.0708 | 0.0077 | 0 | 0.0174 | 0 | 0.0033 | 0.0006 | 0.0004 | 0.0003 |
Bud | 0.0006 | 0 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.001 | 0.0001 | 0.0002 | 0.0002 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 |
Bud Neck | 0.0104 | 0.003 | 0.0054 | 0.0005 | 0.0065 | 0.004 | 0.0022 | 0.0002 | 0.0004 | 0.0005 | 0.0003 | 0.001 | 0.0016 | 0.0002 | 0.0001 | 0.0002 | 0.0004 | 0.0012 |
Bud Periphery | 0.0016 | 0 | 0.0014 | 0.0001 | 0.0007 | 0.0003 | 0.0039 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0 | 0.001 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 |
Bud Site | 0.0035 | 0.0003 | 0.0035 | 0.0002 | 0.0017 | 0.0003 | 0.0054 | 0.0006 | 0.0014 | 0.0007 | 0.0001 | 0 | 0.0098 | 0.0002 | 0.0002 | 0 | 0.0002 | 0.0002 |
Cell Periphery | 0.0008 | 0 | 0.0004 | 0 | 0.0005 | 0.0001 | 0.0007 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0.0007 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0112 | 0.0148 | 0.0046 | 0.0103 | 0.0029 | 0.0016 | 0.0055 | 0.0342 | 0.0122 | 0.0014 | 0.0051 | 0.0008 | 0.0062 | 0.0081 | 0.0034 | 0.0041 | 0.0197 | 0.0024 |
Cytoplasmic Foci | 0.0063 | 0.0001 | 0.0058 | 0.0039 | 0.0023 | 0.0015 | 0.0088 | 0.0023 | 0.0044 | 0.0043 | 0.0059 | 0 | 0.0067 | 0.0005 | 0.01 | 0.0001 | 0.0066 | 0.0009 |
Eisosomes | 0.0007 | 0 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0003 | 0.0002 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0115 | 0.0006 | 0.0013 | 0.001 | 0.0006 | 0.0005 | 0.0046 | 0.0012 | 0.0008 | 0.0017 | 0.001 | 0 | 0.0035 | 0.0002 | 0.0005 | 0.0003 | 0.0014 | 0.0001 |
Endosome | 0.0209 | 0.0004 | 0.0045 | 0.005 | 0.007 | 0.0034 | 0.0228 | 0.0015 | 0.006 | 0.0094 | 0.0069 | 0.0001 | 0.0186 | 0.0006 | 0.0031 | 0.0002 | 0.0241 | 0.0007 |
Golgi | 0.0097 | 0 | 0.0023 | 0.0048 | 0.0044 | 0.0033 | 0.0049 | 0.0031 | 0.0012 | 0.0104 | 0.0023 | 0 | 0.0054 | 0 | 0.0011 | 0 | 0.0116 | 0.0002 |
Lipid Particles | 0.0227 | 0 | 0.0035 | 0.0292 | 0.0135 | 0.0106 | 0.0087 | 0.0012 | 0.0028 | 0.0072 | 0.0181 | 0 | 0.0194 | 0.0002 | 0.0004 | 0 | 0.0032 | 0.0005 |
Mitochondria | 0.0152 | 0.0002 | 0.0049 | 0.0083 | 0.0088 | 0.0022 | 0.0218 | 0.0007 | 0.0011 | 0.0026 | 0.0007 | 0.0001 | 0.0033 | 0.0002 | 0.0095 | 0.0006 | 0.0025 | 0.0002 |
None | 0.0116 | 0.0123 | 0.0029 | 0.0028 | 0.001 | 0.0003 | 0.0206 | 0.0231 | 0.024 | 0.0009 | 0.0221 | 0.0005 | 0.0031 | 0.0335 | 0.0252 | 0.0023 | 0.0784 | 0.0128 |
Nuclear Periphery | 0.0413 | 0.0124 | 0.0081 | 0.0198 | 0.0075 | 0.0063 | 0.0246 | 0.0075 | 0.0066 | 0.0139 | 0.0329 | 0.002 | 0.0099 | 0.0026 | 0.0142 | 0.0261 | 0.0382 | 0.0025 |
Nucleolus | 0.0115 | 0.0065 | 0.0079 | 0.0034 | 0.0341 | 0.0152 | 0.0051 | 0.0051 | 0.0043 | 0.0039 | 0.0051 | 0.0101 | 0.0042 | 0.0139 | 0.0049 | 0.0045 | 0.0057 | 0.0116 |
Nucleus | 0.7629 | 0.9381 | 0.9019 | 0.8517 | 0.8276 | 0.9265 | 0.7842 | 0.9097 | 0.9198 | 0.8643 | 0.8654 | 0.9662 | 0.874 | 0.9236 | 0.9177 | 0.9534 | 0.7814 | 0.9506 |
Peroxisomes | 0.0048 | 0 | 0.0034 | 0.0021 | 0.0031 | 0.0043 | 0.0049 | 0.002 | 0.0004 | 0.0047 | 0.0029 | 0 | 0.0045 | 0 | 0.0008 | 0 | 0.0006 | 0.0013 |
Punctate Nuclear | 0.0119 | 0.0105 | 0.0112 | 0.05 | 0.0669 | 0.0046 | 0.011 | 0.0054 | 0.0055 | 0.0019 | 0.0219 | 0.0189 | 0.005 | 0.0155 | 0.0041 | 0.0068 | 0.0234 | 0.0141 |
Vacuole | 0.005 | 0.0004 | 0.0015 | 0.0004 | 0.002 | 0.0008 | 0.0119 | 0.0011 | 0.0052 | 0.0002 | 0.0007 | 0.0001 | 0.004 | 0.0006 | 0.0005 | 0.0001 | 0.0011 | 0.0002 |
Vacuole Periphery | 0.003 | 0.0001 | 0.0007 | 0.0015 | 0.0007 | 0.0003 | 0.0061 | 0.0002 | 0.0009 | 0.0003 | 0.0009 | 0 | 0.0013 | 0.0001 | 0.0004 | 0.0003 | 0.0007 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 12.8135 | 8.7119 | 7.9011 | 10.6363 | 11.6995 | 12.626 | 9.2979 | 11.2298 | 8.1606 | 13.5094 |
Translational Efficiency | 0.7328 | 0.7284 | 0.7671 | 0.7735 | 0.7418 | 0.662 | 0.8169 | 0.5044 | 0.7582 | 0.6499 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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