Standard name
Human Ortholog
Description Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.07 0.24 0.13 0 0 0 0 0 0 0 0 0 0.18 0.09 0 0.15 0.12 0.11 0.13 0.17 0.21 0.13 0.14 0.16
Bud 0 0.05 0.05 0.07 0.1 0.06 0.11 0.09 0.07 0.09 0.1 0.11 0 0 0 0.05 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.07 0 0.05 0 0 0 0 0 0 0 0 0.07 0.09 0.09 0.09 0.12 0.14 0 0 0 0 0 0
Endoplasmic Reticulum 0.07 0.22 0.08 0 0 0 0 0 0 0 0 0 0.18 0.07 0 0.05 0.05 0.05 0 0 0 0 0 0
Endosome 0.06 0.05 0.14 0.13 0.15 0 0 0.08 0 0 0 0 0.23 0.22 0.38 0.26 0.28 0.34 0.22 0.17 0.15 0.16 0.13 0.15
Golgi 0.12 0.3 0.21 0 0 0 0 0 0 0 0 0 0.35 0.42 0.16 0.3 0.29 0.19 0.47 0.47 0.45 0.42 0.46 0.49
Mitochondria 0.79 0.56 0.62 0.78 0.77 0.92 0.93 0.83 0.92 0.91 0.96 0.9 0.19 0.12 0.27 0.11 0.13 0.11 0.06 0.05 0.05 0.13 0.12 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.05 0.1 0.15 0.05 0 0.08 0 0 0 0.06 0 0.05 0.22 0 0 0 0.05 0 0.09 0 0 0
SpindlePole 0 0 0 0 0 0 0 0.05 0 0 0 0 0.05 0.09 0 0.09 0.1 0.17 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.09 0.07 0.07 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 19 49 49 8 18 10 18 6 3 6 3 4 48 24 9 53 33 23 39 44 99 49 51 69
Bud 9 10 17 21 41 29 72 55 27 35 35 49 7 6 6 18 8 9 1 3 1 3 6 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1
Bud Site 0 0 1 1 2 13 14 11 10 23 10 11 0 0 0 0 1 1 – – – – – –
Cell Periphery 1 1 5 3 1 2 8 6 3 2 1 9 2 2 1 5 3 5 0 0 0 0 0 0
Cytoplasm 9 15 9 17 15 14 13 12 8 4 5 5 18 23 27 32 34 30 1 0 0 4 2 4
Endoplasmic Reticulum 19 44 28 7 9 15 11 8 4 4 5 9 48 17 5 17 15 10 6 6 5 11 10 9
Endosome 16 10 51 40 63 10 11 54 15 9 1 18 61 57 115 92 80 74 67 43 70 61 50 65
Golgi 32 62 78 11 17 0 1 3 0 1 2 3 93 108 47 106 83 41 142 119 208 160 173 210
Mitochondria 203 115 228 246 323 440 613 534 377 344 351 394 50 30 81 40 37 23 18 13 23 50 46 30
Nucleus 0 0 0 0 2 1 4 2 3 1 1 3 1 1 0 8 6 2 0 0 1 0 0 2
Nuclear Periphery 2 0 1 1 0 4 4 6 5 2 5 4 1 0 0 3 1 2 1 0 1 2 1 1
Nucleolus 5 2 1 5 6 10 25 26 12 14 14 15 0 0 4 5 2 3 0 0 0 0 0 0
Peroxisomes 2 2 19 33 64 23 16 51 15 16 8 25 11 14 65 1 12 2 15 7 41 12 14 13
SpindlePole 4 1 12 12 8 5 20 29 13 15 5 7 14 24 8 32 28 37 3 2 2 2 3 1
Vac/Vac Membrane 6 3 4 5 8 2 3 18 10 7 5 13 12 17 27 25 20 12 7 10 3 16 12 10
Unique Cell Count 256 204 365 315 419 478 662 640 408 380 365 438 267 257 299 350 285 217 306 255 465 383 379 429
Labelled Cell Count 327 314 503 410 577 578 833 821 505 483 451 569 366 323 395 437 363 274 306 255 465 383 379 429


Late Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.2 8.6 7.4 6.9 6.7 5.9 5.7 5.8 5.5 5.4 5.0 5.6 9.1 9.0 8.5 8.7 8.4 7.7 9.1 9.3 9.8
Std Deviation (1e-4) 1.0 1.6 1.0 1.5 1.4 1.3 1.4 1.3 1.7 1.1 1.4 3.6 1.5 1.4 1.5 1.7 1.7 1.5 1.5 1.5 1.6
Intensity Change (Log2) – – – -0.1 -0.14 -0.32 -0.38 -0.35 -0.43 -0.45 -0.55 -0.39 0.3 0.28 0.2 0.24 0.19 0.06 0.3 0.33 0.4


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -5.1 -4.6 -6.4 -6.6 -8.4 -7.0 -6.2 -6.6 -7.1 1.6 -1.6 -4.7 0.7 -0.7 -1.0
Bud 1.1 2.7 0.9 3.4 2.3 1.2 2.4 2.6 3.4 -1.3 -1.5 -1.9 0.3 -1.2 -0.3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 2.8 2.4 0 2.6 4.5 2.7 2.6 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0.7 0 0 0 0 0 0
Cytoplasm 2.0 0.9 0.4 -0.5 -0.6 -0.5 -1.5 -1.1 -1.4 2.6 3.6 3.7 3.8 4.8 5.3
Endoplasmic Reticulum -3.2 -3.6 -3.0 -4.8 -5.3 -4.7 -4.5 -4.1 -3.8 3.9 -0.5 -3.5 -1.5 -1.2 -1.4
Endosome -0.5 0.4 -6.6 -7.9 -2.8 -5.1 -5.8 -7.2 -5.0 2.9 2.7 7.3 4.1 4.4 5.7
Golgi -6.9 -7.4 -10.6 -12.2 -11.7 -9.8 -9.4 -9.0 -9.7 3.8 5.5 -1.9 2.7 2.3 -0.7
Mitochondria 4.4 4.5 10.5 12.0 7.5 10.1 9.1 11.2 9.3 -10.9 -12.7 -9.1 -14.1 -12.7 -12.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 3.4 3.6 2.9 3.3 3.4 3.2 0 0 0 0 0 0
Peroxisomes 2.6 4.6 -0.3 -2.4 1.7 -1.0 -0.6 -2.2 0.3 -0.6 0.1 6.4 -4.0 -0.6 -2.7
SpindlePole 0.4 -1.2 -2.3 -0.2 1.0 -0.1 0.5 -1.7 -1.6 1.2 3.2 -0.5 3.3 3.4 5.8
Vacuole 0 0.9 0 0 1.8 1.4 0.8 0 1.8 2.7 3.8 4.8 4.1 4.0 3.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.4989 6.0248 5.6896 5.7449 5.7999 5.5542 4.2853 4.7723 4.9515 3.8722 5.1615 4.4907 3.8865 4.1162 3.673 3.6944 3.3587 3.8478
Actin 0.0473 0.0441 0.0198 0.0015 0.0505 0.0343 0.0583 0.0101 0.0201 0.0323 0.0417 0.0316 0.0301 0.0375 0.0161 0.0209 0.0049 0.0191
Bud 0.0005 0.001 0.0035 0.0001 0.0012 0.0009 0.0008 0.0007 0.0016 0.0014 0.0023 0.0005 0.0006 0.0003 0.0005 0.0004 0.0004 0.0005
Bud Neck 0.0007 0.0005 0.0005 0.0002 0.0005 0.0011 0.0033 0.0007 0.0024 0.0015 0.0025 0.0023 0.0011 0.0004 0.0005 0.0373 0.0004 0.0013
Bud Periphery 0.0004 0.0008 0.0013 0.0001 0.0014 0.0005 0.0008 0.0004 0.0012 0.002 0.0034 0.0008 0.0009 0.0002 0.0004 0.0004 0.0003 0.0003
Bud Site 0.0017 0.0032 0.0014 0.0002 0.0016 0.0007 0.0078 0.0039 0.0061 0.0027 0.005 0.001 0.0043 0.0008 0.0007 0.0024 0.0006 0.0005
Cell Periphery 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0003 0.0001 0.0002 0.0003 0.0004 0.0003 0.0004 0.0001 0.0001 0.0001 0.0001 0.0001
Cytoplasm 0.0037 0.0078 0.005 0.0015 0.0021 0.0048 0.0102 0.011 0.0082 0.0082 0.004 0.0075 0.0059 0.0116 0.0033 0.0026 0.0042 0.0122
Cytoplasmic Foci 0.1669 0.1853 0.1587 0.1652 0.1398 0.1329 0.1265 0.1641 0.1209 0.1302 0.0937 0.1003 0.1522 0.1716 0.1238 0.1429 0.1262 0.1128
Eisosomes 0.0002 0.0001 0.0001 0 0.0001 0.0001 0.0004 0 0.0001 0.0001 0.0008 0.0001 0.0002 0.0003 0.0001 0.0002 0 0.0001
Endoplasmic Reticulum 0.0006 0.0062 0.0005 0.0002 0.0007 0.0005 0.0058 0.0013 0.0035 0.002 0.0057 0.0042 0.0069 0.0009 0.0013 0.0008 0.002 0.0012
Endosome 0.2038 0.2493 0.2047 0.2029 0.1954 0.168 0.3138 0.3342 0.2884 0.3059 0.3047 0.2623 0.2549 0.3277 0.2641 0.2955 0.3247 0.2057
Golgi 0.455 0.4141 0.5046 0.5322 0.4421 0.517 0.3279 0.4221 0.4818 0.4288 0.4336 0.5015 0.4144 0.4184 0.4965 0.3926 0.477 0.5734
Lipid Particles 0.0256 0.0088 0.0095 0.0078 0.0387 0.021 0.0556 0.0147 0.009 0.0242 0.0399 0.0169 0.028 0.0047 0.0141 0.0662 0.0175 0.0175
Mitochondria 0.0231 0.0247 0.0269 0.0393 0.014 0.0418 0.0184 0.0113 0.0201 0.0151 0.0182 0.037 0.0333 0.0098 0.0348 0.0094 0.0091 0.0276
None 0.0034 0.0002 0.004 0.0001 0.0003 0.0022 0.004 0.0002 0.0011 0.0044 0.0015 0.0008 0.0045 0.0003 0.0002 0.0003 0.0015 0.0009
Nuclear Periphery 0.0001 0.0005 0.0002 0 0.0002 0.0001 0.016 0.0008 0.0024 0.0019 0.0215 0.0045 0.0089 0.0007 0.0004 0.0006 0.0012 0.0015
Nucleolus 0.0004 0.0006 0.0006 0 0.0001 0.0003 0.0004 0.0001 0.0002 0.002 0.0012 0.0004 0.0017 0.0001 0.0004 0.0001 0.0001 0.0001
Nucleus 0.0002 0.0001 0.0009 0 0.0001 0.0006 0.0072 0.0006 0.001 0.0032 0.0025 0.0009 0.0014 0.0001 0.0001 0.0001 0.0002 0.0003
Peroxisomes 0.0625 0.0496 0.0543 0.0473 0.1079 0.0696 0.0312 0.0168 0.0252 0.0224 0.005 0.0177 0.038 0.0099 0.037 0.0226 0.023 0.0211
Punctate Nuclear 0.0008 0.0002 0.001 0.0001 0.0007 0.0013 0.0036 0.0005 0.001 0.0061 0.0044 0.0013 0.0053 0.0006 0.0004 0.0008 0.001 0.0008
Vacuole 0.002 0.0015 0.0013 0.0006 0.0012 0.001 0.0047 0.0043 0.0032 0.0035 0.0035 0.0044 0.0046 0.0024 0.0031 0.0021 0.0035 0.0013
Vacuole Periphery 0.0009 0.0013 0.001 0.0007 0.0014 0.0011 0.0031 0.0018 0.0024 0.002 0.0044 0.0037 0.0024 0.0016 0.0022 0.0016 0.0022 0.0015

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 31.432 36.0665 21.1613 28.0282 26.1549 22.506 31.5904 30.6227 31.5214 35.0678
Translational Efficiency 1.023 0.744 1.1557 0.6456 0.9275 1.3639 0.9215 0.7537 0.7455 0.9677

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication
Localization
Cell Percentages Golgi (7%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Cdc50

Cdc50


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cdc50-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available