Standard name
Human Ortholog
Description Cytoplasmic protein required for replication of Brome mosaic virus; S. cerevisiae is a model system for studying replication of positive-strand RNA viruses in their natural hosts
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0.05 0 0 0 0 0 0
Cytoplasm 0.92 0.98 0.95 0.96 0.96 0.89 0.82 0.81 0.76 0.78 0.67 0.77 0.97 1.0 0.99 0.94 0.97 0.9 0.9 0.93 0.89 0.91 0.94 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.19 0 0 0 0.05 0.17 0.31 0.23 0.39 0.29 0.5 0.3 0 0 0 0 0.05 0 0 0 0.06 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 0 2 3 2 0 5 2 2 0 0 0 0 1 0 0 0 0 1
Bud 0 3 0 2 2 1 9 9 11 10 14 10 1 0 0 1 0 0 0 0 0 1 2 5
Bud Neck 0 0 0 0 1 0 1 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 1 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 10 9 4 21 16 13 11 5 14 8 10 11 13 9 8 0 0 1 0 0 1
Cytoplasm 174 124 58 239 467 291 467 500 310 344 238 311 341 489 541 167 117 140 165 121 79 213 392 423
Endoplasmic Reticulum 2 1 1 1 2 1 2 1 0 5 1 1 3 0 0 5 8 10 2 0 0 1 5 4
Endosome 0 0 0 0 0 1 0 2 0 2 0 0 2 0 0 1 0 3 2 0 0 1 1 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 1 3 0 0 0 0 0
Mitochondria 36 5 2 8 23 56 177 142 161 128 177 121 2 1 3 3 6 5 3 1 5 5 6 5
Nucleus 0 1 1 1 1 3 4 7 2 4 1 6 0 2 0 1 5 2 0 1 0 1 1 0
Nuclear Periphery 0 0 0 0 0 1 0 1 0 0 1 1 0 0 0 0 1 1 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 0 1 0 2 0 2 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 1 0 0 0 0 0 0 1 0 0 0 1 0 3 0 0 0 0 0 3
Vac/Vac Membrane 0 0 0 6 10 11 21 26 13 14 8 6 3 1 1 5 3 7 0 3 0 3 2 5
Unique Cell Count 189 126 61 248 485 328 568 617 408 441 357 402 350 491 544 177 121 156 184 131 90 235 418 458
Labelled Cell Count 212 134 62 269 517 369 703 706 515 520 447 476 363 507 556 197 149 182 184 131 90 235 418 458


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 5.7 5.5 6.2 6.3 5.4 5.3 5.9 5.2 5.2 4.7 5.3 7.0 7.2 6.9 6.5 6.6 6.3 6.4 6.8 7.2
Std Deviation (1e-4) 0.8 1.4 0.6 1.4 1.4 1.2 1.5 1.5 1.2 1.1 0.8 0.9 1.1 1.1 1.1 1.9 2.6 2.5 1.2 1.2 1.6
Intensity Change (Log2) – – – 0.17 0.2 -0.03 -0.06 0.09 -0.09 -0.07 -0.22 -0.07 0.35 0.39 0.34 0.24 0.25 0.21 0.22 0.31 0.39


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.5 0.5 -1.5 -2.6 -2.7 -3.4 -3.1 -4.5 -3.2 1.0 3.5 3.3 -0.2 0.5 -1.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 2.8 4.6 3.6 5.5 4.3 0 4.4 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.79 3.6881 3.1322 2.9947 2.8841 3.2039 1.9279 2.8276 2.5546 2.2443 1.5929 2.5025 0.6972 1.7536 1.4097 1.558 1.3186 1.2974
Actin 0.0257 0.0003 0.0034 0.0031 0.0016 0.0032 0.013 0.0003 0.007 0.0007 0.0018 0.0026 0.0173 0.0041 0.0063 0.0231 0.0066 0.016
Bud 0.0014 0.0004 0.0013 0.0004 0.0001 0.0006 0.0012 0.0007 0.0018 0.0007 0.0015 0.002 0.0003 0.0004 0.0008 0.0015 0.0035 0.0002
Bud Neck 0.0016 0.0003 0.0006 0.0007 0.0003 0.0053 0.0101 0.0002 0.0008 0.0182 0.0004 0.0007 0.0006 0.0002 0.0004 0.0007 0.0003 0.0004
Bud Periphery 0.0015 0.0002 0.001 0.0004 0.0001 0.0005 0.001 0.0004 0.0013 0.0012 0.0015 0.0016 0.0003 0.0002 0.0011 0.0014 0.0059 0.0001
Bud Site 0.008 0.0024 0.0051 0.0008 0.0003 0.0006 0.0035 0.0013 0.0057 0.002 0.0011 0.0016 0.0016 0.002 0.0034 0.0022 0.0007 0.0001
Cell Periphery 0.0006 0.0002 0.0002 0.0002 0 0.0001 0.0004 0.0002 0.0003 0.0004 0.0001 0.0001 0.0002 0.0002 0.0003 0.0002 0.0001 0
Cytoplasm 0.7237 0.9102 0.8317 0.7561 0.8073 0.8793 0.6955 0.9079 0.829 0.7534 0.7862 0.8896 0.7352 0.9399 0.8569 0.8879 0.8755 0.902
Cytoplasmic Foci 0.027 0.0179 0.022 0.0315 0.0248 0.0163 0.042 0.0137 0.0273 0.0424 0.0354 0.0182 0.0462 0.0101 0.0296 0.0103 0.0126 0.0153
Eisosomes 0.0003 0 0 0.0001 0 0 0.0001 0.0001 0.0001 0.0001 0 0 0.0001 0 0.0001 0.0003 0.0001 0
Endoplasmic Reticulum 0.0153 0.0024 0.0064 0.0079 0.0014 0.007 0.0256 0.0019 0.003 0.0027 0.0046 0.0054 0.0136 0.0025 0.0066 0.0037 0.0048 0.0086
Endosome 0.0325 0.0133 0.0272 0.0977 0.0259 0.0237 0.046 0.0094 0.0346 0.0492 0.1016 0.0283 0.0621 0.0077 0.0416 0.0167 0.0351 0.0327
Golgi 0.0095 0.0011 0.0037 0.0099 0.0014 0.0024 0.0061 0.0008 0.0088 0.0034 0.0028 0.004 0.0167 0.0014 0.009 0.0027 0.0076 0.0039
Lipid Particles 0.0049 0.0001 0.0016 0.0015 0.0038 0.0005 0.0086 0.0001 0.0083 0.0024 0.0003 0.0009 0.0174 0.0013 0.0058 0.0008 0.0055 0.0064
Mitochondria 0.0102 0.0003 0.0013 0.0081 0.0007 0.0022 0.0065 0.0111 0.0115 0.0312 0.003 0.004 0.0166 0.0002 0.0052 0.0262 0.0042 0.0011
None 0.1115 0.0438 0.0777 0.0475 0.0763 0.0514 0.1142 0.0477 0.0455 0.0434 0.0341 0.0268 0.0498 0.0253 0.02 0.0077 0.0069 0.0047
Nuclear Periphery 0.0047 0.0004 0.0009 0.0043 0.0004 0.0006 0.0043 0.0003 0.001 0.0014 0.0018 0.0045 0.003 0.0004 0.0012 0.0007 0.0125 0.0005
Nucleolus 0.0004 0.0001 0.0001 0.0002 0 0.0002 0.0006 0 0.0003 0.002 0.0001 0.0003 0.0002 0 0.0001 0 0.0005 0
Nucleus 0.0021 0.0011 0.0013 0.0105 0.0007 0.0012 0.003 0.0007 0.0016 0.0027 0.0017 0.0018 0.001 0.0005 0.0007 0.0009 0.0062 0.0005
Peroxisomes 0.0089 0.0001 0.0069 0.0005 0.0499 0.0014 0.0066 0.0002 0.0043 0.0031 0.0006 0.0022 0.0076 0.0015 0.0045 0.0045 0.0007 0.0037
Punctate Nuclear 0.0027 0.0002 0.001 0.001 0.0015 0.0007 0.0027 0.0002 0.0013 0.019 0.0006 0.0006 0.0041 0.0003 0.001 0.0002 0.0011 0.0009
Vacuole 0.0062 0.0048 0.0055 0.0139 0.0028 0.0021 0.0076 0.0026 0.0057 0.0169 0.0157 0.0037 0.0048 0.002 0.0044 0.0071 0.0056 0.002
Vacuole Periphery 0.0012 0.0005 0.001 0.0035 0.0007 0.0007 0.0012 0.0003 0.0011 0.0034 0.005 0.0013 0.0012 0.0003 0.001 0.0013 0.0039 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.1323 6.3403 10.0817 13.882 9.8527 11.1393 14.6531 12.0532 10.8088 15.8373
Translational Efficiency 0.9737 1.7575 1.0622 0.8354 1.25 0.9488 0.552 0.9059 1.1122 0.8164

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Cytoplasmic protein required for replication of Brome mosaic virus; S. cerevisiae is a model system for studying replication of positive-strand RNA viruses in their natural hosts
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Oca4

Oca4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Oca4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available