Standard name
Human Ortholog
Description Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.13 0.29 0.38 0.16 0.44 0.37 0.53 – 0.58 0.58 0.62 0.67 0.52 0.75 0.89 0.22 0.18 0.18 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0 0 – 0 0 0 0 0.05 0 0 0 0 0 0.05 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0.14 0 0.05 0 0.14 0 – 0 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.14 0.1 0.33 0.16 0.07 0.19 0.15 – 0.11 0.1 0.07 0.06 0 0 0 0.3 0.23 0.28 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.77 0.71 0.57 0.82 0.64 0.56 0.53 – 0.5 0.49 0.41 0.41 0.58 0.34 0.16 0.6 0.66 0.72 0.88 0.86 0.92 0.91 0.87 0.83
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2
Bud 0 1 0 0 0 0 1 – 1 2 1 5 1 0 0 0 0 0 0 2 1 2 3 5
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 31 45 32 6 72 50 117 – 110 125 124 156 150 366 417 34 23 12 0 6 0 2 5 16
Endoplasmic Reticulum 1 0 0 0 1 0 1 – 0 2 0 1 4 1 0 8 3 1 0 0 0 0 2 3
Endosome 8 2 2 1 8 3 4 – 3 2 4 4 15 12 9 3 2 3 14 4 2 5 19 19
Golgi 0 0 1 0 1 0 0 – 0 0 0 0 0 0 0 0 0 0 4 0 0 0 2 0
Mitochondria 20 22 2 2 1 19 5 – 5 11 9 14 1 0 1 0 4 3 6 3 0 3 5 12
Nucleus 35 15 28 6 11 25 34 – 20 21 15 14 7 3 0 47 30 19 2 1 0 4 7 2
Nuclear Periphery 0 1 0 0 1 0 1 – 1 1 0 3 0 0 0 1 0 0 1 1 0 3 1 6
Nucleolus 1 0 0 0 0 1 0 – 1 1 1 0 0 0 1 0 2 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 1 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
SpindlePole 0 0 0 0 1 0 1 – 0 0 0 1 1 2 0 0 0 0 0 1 1 1 2 3
Vac/Vac Membrane 187 110 48 31 105 75 118 – 95 105 83 96 168 168 73 93 85 49 234 144 89 253 410 389
Unique Cell Count 243 154 84 38 164 134 222 189 214 200 234 288 489 467 156 129 68 268 168 97 280 469 469
Labelled Cell Count 283 196 113 46 202 173 283 236 270 237 297 347 552 501 186 149 87 268 168 97 280 469 469


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 5.2 5.9 5.5 8.1 5.9 7.0 – 6.2 6.6 6.3 7.9 5.7 5.4 5.2 10.0 9.7 9.9 5.4 5.4 5.5
Std Deviation (1e-4) 1.2 1.2 1.4 1.1 1.6 1.7 1.7 – 1.7 1.8 1.6 22.5 1.4 1.4 1.5 3.0 2.6 2.5 1.5 1.9 1.8
Intensity Change (Log2) – – – -0.1 0.45 0.0 0.25 – 0.08 0.16 0.08 0.42 -0.06 -0.14 -0.17 0.76 0.72 0.75 -0.14 -0.12 -0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.5 0.9 -0.1 2.3 – 3.1 3.2 3.7 4.6 2.3 6.8 11.1 -2.7 -3.3 -2.8
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 2.9 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus -2.0 -5.5 -2.5 -3.5 – -4.6 -4.9 -5.5 -6.4 -8.5 0 0 -0.5 -1.6 -0.7
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 2.6 1.1 -0.2 -0.6 – -1.1 -1.3 -2.4 -2.5 0.2 -4.0 -8.5 0.4 1.3 1.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.6271 1.4993 0.966 0.3006 0.2599 0.8418 0.8552 2.7486 2.4571 2.051 2.0049 2.4009 2.9592 5.636 4.9729 4.8356 4.751 5.0207
Actin 0.0035 0 0.0017 0 0.004 0 0.0025 0.0001 0.0025 0.0007 0.0002 0.0003 0.0006 0 0.0005 0 0.0024 0
Bud 0.0003 0.0002 0.0003 0.0002 0.0005 0.0003 0.0011 0.0016 0.0014 0.0011 0.0003 0.0009 0.0002 0.0002 0.0003 0.0002 0.0002 0.0003
Bud Neck 0.0005 0.0005 0.0008 0.001 0.0013 0.001 0.0012 0.0012 0.0009 0.0018 0.0012 0.0016 0.001 0.0006 0.0007 0.0005 0.0011 0.0011
Bud Periphery 0.0006 0.0005 0.0007 0.0006 0.0012 0.0009 0.0022 0.0009 0.0017 0.0017 0.0004 0.0012 0.0006 0.0005 0.0008 0.0004 0.0006 0.0008
Bud Site 0.0007 0.0005 0.0014 0.0005 0.0012 0.0005 0.0015 0.01 0.0037 0.0059 0.001 0.0011 0.0012 0.0005 0.0008 0.0003 0.0006 0.0006
Cell Periphery 0.001 0.002 0.0014 0.0023 0.0025 0.0025 0.0011 0.0014 0.0017 0.0016 0.0008 0.0018 0.0013 0.0017 0.0016 0.0008 0.0024 0.002
Cytoplasm 0.0035 0.0081 0.003 0.0018 0.0049 0.002 0.0032 0.0101 0.0126 0.0027 0.0041 0.0046 0.0082 0.0023 0.0031 0.0015 0.0016 0.0035
Cytoplasmic Foci 0.0191 0.0092 0.0084 0.002 0.0065 0.0028 0.0173 0.0188 0.0089 0.0077 0.0218 0.0188 0.009 0.0048 0.0043 0.0031 0.0026 0.0033
Eisosomes 0 0 0 0 0.0001 0 0.0001 0 0.0001 0 0 0 0.0001 0 0.0001 0 0.0013 0
Endoplasmic Reticulum 0.002 0.0027 0.002 0.0039 0.0011 0.0011 0.003 0.0033 0.005 0.0008 0.0009 0.0015 0.0032 0.0037 0.0028 0.0007 0.0012 0.002
Endosome 0.3197 0.1303 0.2384 0.1384 0.1087 0.1111 0.3318 0.2 0.2425 0.2416 0.3112 0.166 0.2719 0.1187 0.2265 0.1747 0.1299 0.13
Golgi 0.0097 0.0024 0.0105 0.002 0.0049 0.0027 0.0167 0.0067 0.0099 0.0145 0.0444 0.0064 0.0084 0.0036 0.0071 0.0033 0.0038 0.0029
Lipid Particles 0.0261 0.0052 0.0169 0.0056 0.0095 0.0122 0.0459 0.0124 0.0086 0.005 0.0056 0.0152 0.0175 0.0079 0.0063 0.0041 0.01 0.0089
Mitochondria 0.0171 0.0071 0.0452 0.0056 0.0032 0.0173 0.048 0.0211 0.0674 0.0048 0.0034 0.005 0.0256 0.0017 0.0351 0.0334 0.0047 0.0082
None 0.0036 0.0014 0.0005 0.0002 0.0144 0.0012 0.0006 0.0009 0.0041 0.0001 0.0002 0.0003 0.0122 0.0004 0.005 0.0002 0.0046 0.0005
Nuclear Periphery 0.0093 0.0068 0.009 0.0103 0.0035 0.0029 0.0036 0.0131 0.0107 0.0023 0.0017 0.0029 0.0058 0.006 0.0079 0.0013 0.0037 0.0078
Nucleolus 0.007 0.0044 0.0044 0.0045 0.003 0.0049 0.0015 0.0076 0.0037 0.0033 0.0025 0.0048 0.0035 0.001 0.0016 0.0011 0.0035 0.0022
Nucleus 0.007 0.0034 0.0072 0.0027 0.0043 0.0043 0.0016 0.0138 0.0035 0.0279 0.003 0.0042 0.0042 0.002 0.0025 0.0014 0.0023 0.0027
Peroxisomes 0.0048 0.0007 0.0039 0.0005 0.0018 0.007 0.0053 0.001 0.0023 0.0017 0.0011 0.0009 0.0026 0.0003 0.0006 0.0005 0.0013 0.0004
Punctate Nuclear 0.0018 0.0002 0.0003 0.0001 0.0177 0.0002 0.0027 0.0051 0.0002 0.0001 0.0002 0.0001 0.0006 0.0001 0.0001 0 0.0011 0.0001
Vacuole 0.4174 0.6184 0.4689 0.6505 0.6822 0.6295 0.3841 0.5322 0.4555 0.5613 0.4965 0.6418 0.461 0.6301 0.5242 0.6365 0.6898 0.6351
Vacuole Periphery 0.1452 0.1961 0.175 0.1672 0.1235 0.1955 0.1251 0.1387 0.1534 0.1131 0.0994 0.1205 0.1613 0.214 0.1683 0.136 0.1313 0.1878

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 34.0851 7.086 10.5163 19.2992 27.7489 22.1826 16.4788 24.1784 18.1741 34.6412
Translational Efficiency 2.2616 4.5265 1.9389 1.8152 1.9582 3.8262 1.8349 1.2134 1.8962 1.6457

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication
Localization
Cell Percentages vacuole (91%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Grx6

Grx6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Grx6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available