Standard name | |
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Human Ortholog | |
Description | Enoyl reductase; catalyzes last step in each cycle of very long chain fatty acid elongation; localizes to ER, highly enriched in a structure marking nuclear-vacuolar junctions; coimmunoprecipitates with elongases Elo2p and Elo3p; protein increases in abundance and relative distribution to ER foci increases upon DNA replication stress; human homolog TECR implicated in nonsyndromic mental retardation, can complement yeast mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 17 | 11 | 11 | 14 | 17 | 36 | 30 | – | 28 | 45 | 35 | 32 | 22 | 18 | 3 | 8 | 9 | 8 | 12 | 5 | 8 | 5 | 6 |
Bud | 0 | 1 | 1 | 1 | 0 | 2 | 11 | 3 | – | 9 | 21 | 11 | 6 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 4 | 2 | 7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 1 | 1 | 2 | 7 | 9 | – | 2 | 5 | 6 | 26 | 39 | 55 | 10 | 11 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 25 | 11 | 10 | 22 | 25 | 50 | 65 | – | 125 | 65 | 91 | 26 | 40 | 51 | 40 | 36 | 31 | 0 | 3 | 0 | 1 | 1 | 2 |
Endoplasmic Reticulum | 0 | 209 | 150 | 75 | 119 | 120 | 109 | 156 | – | 91 | 88 | 56 | 199 | 190 | 217 | 11 | 2 | 4 | 8 | 369 | 251 | 253 | 355 | 452 |
Endosome | 0 | 2 | 5 | 12 | 16 | 13 | 34 | 40 | – | 66 | 60 | 70 | 5 | 13 | 8 | 2 | 1 | 1 | 15 | 27 | 8 | 17 | 23 | 30 |
Golgi | 1 | 91 | 72 | 58 | 20 | 4 | 5 | 5 | – | 11 | 6 | 3 | 138 | 173 | 130 | 214 | 116 | 125 | 1 | 4 | 1 | 7 | 7 | 12 |
Mitochondria | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 2 | – | 1 | 6 | 9 | 0 | 0 | 1 | 10 | 2 | 3 | 2 | 2 | 1 | 2 | 3 | 6 |
Nucleus | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 0 | 3 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 4 | 3 | 2 | 4 | 2 | 5 | – | 2 | 1 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 3 | 35 | 18 | 22 | 29 | 19 |
Nucleolus | 11 | 59 | 44 | 26 | 16 | 16 | 20 | 26 | – | 34 | 32 | 37 | 69 | 72 | 62 | 22 | 9 | 14 | 7 | 1 | 0 | 1 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 2 | 2 | 0 | 0 | 0 | 4 | 1 | – | 4 | 1 | 1 | 4 | 7 | 16 | 4 | 0 | 2 | 7 | 5 | 1 | 1 | 0 | 4 |
Vac/Vac Membrane | 2 | 55 | 16 | 12 | 30 | 20 | 63 | 52 | – | 90 | 45 | 69 | 12 | 31 | 41 | 48 | 30 | 28 | 1 | 29 | 8 | 18 | 42 | 64 |
Unique Cell Count | 18 | 389 | 239 | 148 | 196 | 195 | 274 | 335 | 382 | 302 | 323 | 396 | 455 | 478 | 307 | 191 | 187 | 74 | 502 | 303 | 342 | 476 | 617 | |
Labelled Cell Count | 20 | 463 | 316 | 209 | 241 | 224 | 343 | 394 | 465 | 375 | 392 | 518 | 590 | 601 | 364 | 217 | 222 | 74 | 502 | 303 | 342 | 476 | 617 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 12.2 | 29.7 | 24.0 | 22.4 | 19.5 | 16.3 | 15.4 | 14.8 | – | 16.1 | 13.8 | 14.7 | 26.3 | 25.1 | 23.9 | 28.2 | 28.4 | 28.7 | 30.7 | 30.5 | 30.9 |
Std Deviation (1e-4) | 5.1 | 5.8 | 4.3 | 4.1 | 3.1 | 2.8 | 2.8 | 2.7 | – | 7.2 | 2.7 | 5.5 | 5.0 | 5.2 | 4.8 | 5.9 | 6.2 | 6.5 | 9.5 | 6.4 | 6.2 |
Intensity Change (Log2) | – | – | – | -0.1 | -0.3 | -0.56 | -0.64 | -0.7 | – | -0.58 | -0.8 | -0.71 | 0.13 | 0.06 | -0.01 | 0.23 | 0.24 | 0.26 | 0.35 | 0.34 | 0.36 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.2 | 1.1 | 1.7 | 3.3 | 2.0 | – | 1.4 | 3.9 | 2.7 | 1.7 | 0.1 | -0.5 | -2.7 | -0.2 | 0.1 |
Bud | 0 | 0 | 0 | 2.7 | 0 | – | 0 | 3.8 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 4.0 | 4.7 | 5.5 | 0 | 0 | 0 |
Cytoplasm | 0.9 | 2.6 | 3.1 | 4.8 | 5.2 | – | 8.2 | 5.6 | 7.2 | 1.0 | 2.0 | 2.7 | 3.4 | 4.7 | 4.1 |
Endoplasmic Reticulum | -2.3 | -0.4 | -0.3 | -5.2 | -3.8 | – | -9.7 | -7.8 | -11.0 | -3.1 | -5.3 | -4.4 | -15.0 | -13.3 | -12.9 |
Endosome | 2.8 | 2.9 | 2.4 | 4.4 | 4.3 | – | 5.8 | 6.3 | 6.7 | 0 | 0.6 | 0 | 0 | 0 | 0 |
Golgi | 1.8 | -5.1 | -7.7 | -9.0 | -9.9 | – | -9.7 | -9.3 | -10.1 | 1.2 | 2.1 | -0.8 | 9.2 | 6.4 | 7.5 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -0.2 | -3.1 | -3.1 | -3.8 | -3.8 | – | -3.5 | -2.6 | -2.3 | -0.3 | -0.9 | -1.9 | -4.0 | -4.3 | -3.3 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2.0 | 0 | 0 | 0 |
Vacuole | 0.5 | 2.9 | 1.3 | 5.1 | 3.2 | – | 5.4 | 3.0 | 4.8 | -2.2 | 0.1 | 0.9 | 3.2 | 3.0 | 2.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 19.8473 | 23.6081 | 21.4662 | 17.2076 | 17.4962 | 22.3353 | 27.7752 | 29.5667 | 28.0033 | 26.0101 | 24.8382 | 27.8845 | 28.3722 | 28.2966 | 28.497 | 27.0819 | 26.6936 | 27.8409 |
Actin | 0.0046 | 0.0001 | 0.0029 | 0.0202 | 0.0274 | 0.0004 | 0.0045 | 0.0001 | 0.0019 | 0.0002 | 0.0005 | 0.0002 | 0.0014 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 |
Bud | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 |
Bud Neck | 0.0003 | 0.0022 | 0.0007 | 0.0046 | 0.0029 | 0.0017 | 0.0006 | 0.0013 | 0.0007 | 0.0005 | 0.0009 | 0.0019 | 0.0008 | 0.0032 | 0.0007 | 0.0015 | 0.0027 | 0.0028 |
Bud Periphery | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0004 | 0.0003 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0.0003 | 0.0003 | 0.0004 | 0.0001 | 0.0004 | 0.0008 | 0.0004 |
Bud Site | 0.0005 | 0.0006 | 0.0002 | 0.0022 | 0.0008 | 0.0001 | 0.0009 | 0.0005 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0001 |
Cell Periphery | 0.0141 | 0.0571 | 0.0338 | 0.0237 | 0.0111 | 0.025 | 0.0207 | 0.0642 | 0.0371 | 0.0093 | 0.019 | 0.0296 | 0.0482 | 0.0848 | 0.0559 | 0.0354 | 0.0816 | 0.0474 |
Cytoplasm | 0.0069 | 0.0095 | 0.0098 | 0.0098 | 0.0615 | 0.0037 | 0.0094 | 0.0073 | 0.005 | 0.0044 | 0.0038 | 0.0028 | 0.0083 | 0.0074 | 0.0071 | 0.0069 | 0.0106 | 0.0047 |
Cytoplasmic Foci | 0.0151 | 0.0074 | 0.0069 | 0.0075 | 0.0119 | 0.0038 | 0.0085 | 0.0056 | 0.0066 | 0.0051 | 0.0113 | 0.0041 | 0.0041 | 0.0054 | 0.0051 | 0.005 | 0.0025 | 0.0038 |
Eisosomes | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.001 | 0.0001 | 0.0008 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.2388 | 0.2388 | 0.3503 | 0.4798 | 0.3098 | 0.234 | 0.212 | 0.1746 | 0.3036 | 0.3887 | 0.2082 | 0.1928 | 0.2186 | 0.1438 | 0.3416 | 0.3655 | 0.2843 | 0.1958 |
Endosome | 0.0278 | 0.0062 | 0.0157 | 0.015 | 0.0555 | 0.0094 | 0.0178 | 0.0082 | 0.0096 | 0.0174 | 0.0332 | 0.0087 | 0.009 | 0.0049 | 0.0046 | 0.0085 | 0.0054 | 0.0043 |
Golgi | 0.006 | 0.002 | 0.0029 | 0.0035 | 0.0111 | 0.0027 | 0.0039 | 0.0023 | 0.0032 | 0.0039 | 0.014 | 0.0034 | 0.0023 | 0.0016 | 0.0013 | 0.0017 | 0.0012 | 0.0021 |
Lipid Particles | 0.3037 | 0.4143 | 0.3465 | 0.1893 | 0.3142 | 0.3566 | 0.3912 | 0.4428 | 0.4333 | 0.41 | 0.4665 | 0.4339 | 0.3023 | 0.3565 | 0.3086 | 0.2714 | 0.2897 | 0.3839 |
Mitochondria | 0.0033 | 0.0001 | 0.0003 | 0.0069 | 0.0098 | 0.0002 | 0.0016 | 0.0002 | 0.0015 | 0.0009 | 0.0036 | 0.0056 | 0.0011 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
None | 0.0025 | 0.0002 | 0.0005 | 0.0007 | 0.01 | 0.0004 | 0.0017 | 0.0003 | 0.0004 | 0.0005 | 0.0006 | 0.0003 | 0.0011 | 0.0003 | 0.0003 | 0.0002 | 0.0004 | 0.0002 |
Nuclear Periphery | 0.293 | 0.0945 | 0.1362 | 0.1564 | 0.083 | 0.2686 | 0.2313 | 0.111 | 0.1177 | 0.0754 | 0.1234 | 0.2216 | 0.2418 | 0.1085 | 0.1472 | 0.1435 | 0.1594 | 0.1943 |
Nucleolus | 0.0042 | 0.0288 | 0.0054 | 0.0014 | 0.0035 | 0.0126 | 0.0171 | 0.0393 | 0.0061 | 0.0314 | 0.0271 | 0.0111 | 0.0284 | 0.0814 | 0.0196 | 0.0153 | 0.0137 | 0.0248 |
Nucleus | 0.0139 | 0.0375 | 0.0111 | 0.0149 | 0.0131 | 0.0214 | 0.0123 | 0.0153 | 0.0065 | 0.0105 | 0.0127 | 0.0141 | 0.0297 | 0.0473 | 0.0133 | 0.0243 | 0.0184 | 0.0282 |
Peroxisomes | 0.0027 | 0.0001 | 0.0016 | 0.0105 | 0.0056 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 |
Punctate Nuclear | 0.0078 | 0.0045 | 0.0062 | 0.0018 | 0.0087 | 0.008 | 0.0082 | 0.0058 | 0.0035 | 0.0042 | 0.008 | 0.007 | 0.009 | 0.0087 | 0.0084 | 0.0077 | 0.0123 | 0.0089 |
Vacuole | 0.0414 | 0.0904 | 0.0614 | 0.0413 | 0.0506 | 0.043 | 0.0463 | 0.1155 | 0.0573 | 0.0282 | 0.0512 | 0.0537 | 0.0833 | 0.1396 | 0.0817 | 0.106 | 0.112 | 0.0907 |
Vacuole Periphery | 0.0128 | 0.005 | 0.0071 | 0.0101 | 0.0075 | 0.0078 | 0.0103 | 0.0051 | 0.005 | 0.0089 | 0.0148 | 0.0083 | 0.0094 | 0.0052 | 0.0039 | 0.006 | 0.0044 | 0.0072 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 138.9604 | 102.5493 | 91.7859 | 127.4467 | 103.5009 | 82.7391 | 104.222 | 110.0233 | 94.7146 | 140.3373 |
Translational Efficiency | 1.4727 | 1.8185 | 1.9836 | 1.3006 | 1.6707 | 2.5882 | 1.7038 | 1.4529 | 1.7361 | 1.3837 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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