Standard name
Human Ortholog
Description Protein with similarity to Emp24p and Erv25p; member of the p24 family involved in ER to Golgi transport
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0.1 0.05 0.06 0 0.05 – 0.07 0 0.07 0 0 0 0.07 0.1 0.1 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.06 0 0.08 0.13 0.07 0.06 0.1 0.07 – 0.14 0.15 0.15 0.11 0.15 0.18 0.32 0.42 0.43 0 0 0 0 0 0
Cytoplasm 0.14 0.09 0.1 0.13 0.17 0.2 0.15 0.16 – 0.19 0.15 0.18 0.09 0.13 0.12 0.26 0.32 0.25 0 0 0 0 0 0
Endoplasmic Reticulum 0.55 0.9 0.86 0.76 0.59 0.71 0.63 0.52 – 0.59 0.4 0.44 0.89 0.75 0.82 0 0 0 0.91 0.92 0.94 0.84 0.88 0.86
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.21 0.08 0.12 0 0 0 0 0 0
Mitochondria 0 0 0.06 0.1 0.16 0.16 0.24 0.23 – 0.22 0.38 0.39 0 0 0 0.07 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.35 0.07 0 0.07 0.17 0 0.09 0.17 – 0.11 0.14 0.08 0 0.1 0 0.25 0.1 0.16 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 15 11 18 13 16 16 19 – 24 9 24 24 22 15 11 14 10 8 4 4 9 8 9
Bud 0 1 0 1 2 3 2 5 – 2 3 5 2 0 0 1 0 0 2 4 0 1 1 4
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 2 1 0 1 1 3
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 35 22 28 23 20 16 37 27 – 48 32 48 68 89 119 52 57 43 3 3 3 4 1 8
Cytoplasm 83 43 33 24 46 58 55 58 – 68 33 61 54 76 82 42 44 25 10 10 3 5 13 12
Endoplasmic Reticulum 338 440 287 138 161 203 225 194 – 206 87 145 536 433 536 3 2 2 678 481 325 291 438 492
Endosome 8 0 0 1 7 0 1 2 – 0 2 1 0 1 1 1 0 0 3 1 1 2 2 3
Golgi 12 2 3 2 3 0 0 0 – 2 2 5 2 19 3 35 11 12 8 2 0 6 4 1
Mitochondria 8 3 21 19 44 47 86 86 – 78 82 130 0 0 0 12 4 3 9 2 3 9 9 7
Nucleus 1 1 0 0 1 2 5 1 – 4 2 1 0 2 1 0 1 0 0 0 0 0 0 0
Nuclear Periphery 8 2 9 1 6 9 11 10 – 16 7 14 6 7 6 2 2 0 4 1 1 1 1 1
Nucleolus 3 0 1 1 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0
Vac/Vac Membrane 216 32 7 12 46 12 31 64 – 38 30 26 15 58 26 41 14 16 7 5 0 11 13 24
Unique Cell Count 612 491 332 182 273 287 359 371 350 217 330 603 576 656 164 137 99 745 522 348 349 500 573
Labelled Cell Count 720 561 400 240 350 366 469 466 486 289 460 708 707 789 201 150 111 745 522 348 349 500 573


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.9 12.1 9.9 10.1 9.6 9.3 8.7 9.1 – 9.0 8.7 8.9 13.4 14.6 14.3 16.2 16.5 16.7 12.5 12.1 12.2
Std Deviation (1e-4) 1.9 2.1 1.5 1.8 1.6 1.6 1.5 1.8 – 1.8 1.8 1.8 2.3 2.6 2.6 3.1 3.2 3.0 2.0 1.9 1.7
Intensity Change (Log2) – – – 0.02 -0.04 -0.09 -0.19 -0.13 – -0.14 -0.18 -0.15 0.43 0.55 0.53 0.71 0.74 0.75 0.33 0.29 0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 3.1 0.9 1.4 0.8 1.2 – 2.1 0.5 2.3 0.5 0.4 -1.0 1.7 3.0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 1.5 -0.5 -1.4 0.8 -0.6 – 2.2 2.3 2.5 1.4 3.0 4.0 6.6 8.5 8.2
Cytoplasm 1.1 2.5 3.6 2.1 2.2 – 3.5 1.9 3.1 -0.5 1.5 1.2 4.6 5.9 3.9
Endoplasmic Reticulum -3.0 -7.7 -4.8 -7.1 -9.7 – -8.0 -11.4 -11.5 1.1 -4.0 -1.9 -18.0 -17.2 -15.7
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 2.3 0 8.1 0 0
Mitochondria 1.7 3.9 4.0 6.4 6.2 – 5.9 9.2 10.1 -6.2 -6.1 -6.5 0.4 -1.5 -1.3
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 -0.4 0.3 0.3 0 – 1.3 0.4 1.1 -2.0 -1.6 -2.2 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 2.6 6.4 1.5 3.8 6.7 – 4.6 5.4 3.4 0.4 4.5 1.5 8.1 3.9 5.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.8113 9.2954 9.8396 9.7079 8.5736 8.9952 10.9086 11.9321 11.8135 11.3264 10.266 10.9766 8.6404 9.3471 9.691 9.2139 8.7756 8.8494
Actin 0.0002 0.0026 0.0013 0.0001 0.001 0.0004 0.0082 0.0033 0.0009 0 0.0447 0.0006 0.001 0.0004 0.0006 0.0002 0.0026 0.0002
Bud 0.0001 0.0001 0.0008 0 0.0006 0.0029 0 0 0 0 0.0002 0 0.0001 0 0 0 0.0001 0
Bud Neck 0.0002 0.0005 0.0007 0.0009 0.0023 0.0014 0.0003 0.0001 0.0001 0 0.0039 0.0004 0.0004 0.0007 0.0001 0.0031 0.01 0.0019
Bud Periphery 0.0001 0.0003 0.0009 0.0001 0.0014 0.0008 0.0001 0 0 0 0.0003 0.0001 0.0001 0.0001 0 0 0.0002 0
Bud Site 0.0004 0.0037 0.0028 0 0.0003 0.0001 0.0001 0.0001 0 0 0.0005 0.0001 0.0016 0.0014 0.0001 0 0.0001 0
Cell Periphery 0.0027 0.003 0.0021 0.001 0.0007 0.0011 0.0007 0.0041 0.0006 0.0003 0.0005 0.0005 0.0016 0.0038 0.0006 0.0016 0.002 0.0005
Cytoplasm 0.0518 0.097 0.0664 0.034 0.0757 0.0571 0.017 0.0249 0.0121 0.0016 0.017 0.0182 0.0411 0.0913 0.046 0.0322 0.0411 0.0443
Cytoplasmic Foci 0.0013 0.0016 0.0016 0.0002 0.0008 0.0006 0.001 0.0017 0.0006 0 0.0162 0.0009 0.0012 0.0011 0.0007 0.0003 0.0007 0.0004
Eisosomes 0 0.0001 0.0001 0 0 0 0.0005 0 0 0 0.0005 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.9091 0.8361 0.8821 0.9394 0.8741 0.9124 0.9431 0.9345 0.9766 0.9919 0.8556 0.9653 0.9234 0.8716 0.9386 0.95 0.9274 0.9391
Endosome 0.0021 0.0068 0.0029 0.0016 0.0029 0.0037 0.0046 0.004 0.001 0.0011 0.0131 0.0041 0.0036 0.0032 0.0023 0.0019 0.0027 0.0022
Golgi 0.0002 0.0021 0.0008 0.0001 0.0003 0.0002 0.0027 0.0022 0.0003 0.0002 0.0116 0.0008 0.001 0.0005 0.0006 0.0001 0.0005 0.0001
Lipid Particles 0.0012 0.0053 0.0035 0.0004 0.0012 0.0013 0.0035 0.0095 0.0018 0.0001 0.0073 0.0023 0.002 0.0078 0.0014 0.0015 0.0022 0.0007
Mitochondria 0.0001 0.0004 0.0007 0.0001 0.0001 0.0003 0.0082 0.0002 0 0.0001 0.0183 0.0001 0.0003 0.0001 0.0001 0.0001 0.0006 0.0001
None 0.0008 0.0017 0.0014 0.0001 0.001 0.0006 0.0005 0.0003 0.0001 0 0.0004 0.0007 0.0056 0.0006 0.0007 0.0002 0.0004 0.0004
Nuclear Periphery 0.02 0.0189 0.0168 0.0049 0.0208 0.01 0.0068 0.0122 0.0046 0.0032 0.0023 0.0036 0.0123 0.0087 0.0051 0.0055 0.0054 0.0059
Nucleolus 0.0001 0.0002 0.0006 0 0.0004 0.0001 0 0 0 0 0 0 0 0.0001 0 0 0 0
Nucleus 0.0038 0.0106 0.0057 0.0011 0.0102 0.003 0.0005 0.0006 0.0004 0.0001 0.0002 0.0003 0.0013 0.003 0.0008 0.0007 0.0009 0.0015
Peroxisomes 0 0 0 0 0 0 0.0001 0.0001 0 0 0.0056 0 0.0001 0 0 0 0 0
Punctate Nuclear 0.0013 0.0011 0.0046 0 0.0016 0.0003 0.0003 0.0001 0 0 0.0004 0.0002 0.0001 0.0004 0.0002 0.0001 0.0001 0.0001
Vacuole 0.0041 0.0072 0.0038 0.0156 0.004 0.0032 0.0017 0.0018 0.0009 0.001 0.0011 0.0016 0.0028 0.005 0.0022 0.0022 0.0026 0.0025
Vacuole Periphery 0.0004 0.0005 0.0004 0.0003 0.0004 0.0004 0.0003 0.0005 0.0001 0.0002 0.0002 0.0002 0.0003 0.0002 0.0001 0.0002 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 64.2839 27.6418 11.875 24.1248 36.9258 48.7962 20.5937 17.3742 20.4705 50.5293
Translational Efficiency 1.014 0.6252 1.2203 1.1417 1.3051 1.5051 1.0924 0.9327 1.2365 1.1104

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein with similarity to Emp24p and Erv25p; member of the p24 family involved in ER to Golgi transport
Localization
Cell Percentages ER (98%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Erp3

Erp3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Erp3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available