Standard name | |
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Human Ortholog | |
Description | Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 4 | 3 | 10 | 10 |
Bud | 1 | 1 | 6 | 4 | 2 | 1 | 5 | 7 | 9 | 1 | 1 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 4 | 6 | 10 | 25 |
Bud Neck | 0 | 0 | 1 | 4 | 1 | 3 | 3 | 2 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 3 | 5 | 11 |
Bud Site | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | – | 1 | 2 | 0 | 0 | 2 | 0 | 2 | 4 |
Cytoplasm | 47 | 159 | 211 | 175 | 230 | 378 | 297 | 386 | 214 | 168 | 49 | 26 | 276 | 586 | 527 | – | 146 | 70 | 82 | 166 | 175 | 284 | 410 | 353 |
Endoplasmic Reticulum | 1 | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 1 | 1 | – | 16 | 9 | 8 | 0 | 1 | 3 | 4 | 5 |
Endosome | 2 | 0 | 0 | 0 | 4 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 19 | 14 | 2 | – | 2 | 0 | 4 | 1 | 2 | 2 | 9 | 8 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 2 | 0 | 0 | 2 | 3 | 4 |
Mitochondria | 16 | 23 | 39 | 75 | 105 | 166 | 138 | 189 | 225 | 152 | 44 | 35 | 9 | 5 | 3 | – | 2 | 3 | 15 | 9 | 27 | 21 | 25 | 38 |
Nucleus | 1 | 2 | 1 | 3 | 6 | 2 | 5 | 2 | 12 | 5 | 0 | 2 | 29 | 2 | 1 | – | 4 | 1 | 2 | 8 | 2 | 14 | 6 | 9 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 3 | 1 | 0 | 0 | 1 | 2 | – | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 3 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 1 | 5 | 2 | 1 | 3 | 5 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | – | 1 | 0 | 6 | 4 | 9 | 24 | 18 | 30 |
Vac/Vac Membrane | 3 | 15 | 8 | 5 | 19 | 10 | 4 | 11 | 7 | 7 | 1 | 4 | 83 | 73 | 20 | – | 40 | 8 | 22 | 27 | 26 | 28 | 17 | 52 |
Unique Cell Count | 62 | 189 | 251 | 223 | 326 | 461 | 371 | 508 | 367 | 276 | 80 | 51 | 393 | 646 | 546 | 184 | 80 | 154 | 223 | 271 | 410 | 539 | 578 | |
Labelled Cell Count | 73 | 202 | 269 | 271 | 370 | 563 | 459 | 608 | 472 | 340 | 98 | 68 | 419 | 684 | 557 | 212 | 93 | 154 | 223 | 271 | 410 | 539 | 578 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.6 | 3.6 | 3.2 | 3.4 | 3.3 | 3.4 | 2.5 | 3.0 | 3.0 | 2.5 | 3.8 | 3.0 | 4.5 | 4.1 | 4.1 | 4.6 | 5.8 | 5.8 | 3.8 | 4.1 | 4.9 |
Std Deviation (1e-4) | 0.6 | 0.5 | 0.9 | 1.4 | 1.0 | 2.7 | 1.0 | 1.0 | 1.4 | 0.9 | 2.9 | 1.7 | 1.0 | 1.1 | 1.1 | 1.4 | 1.6 | 1.4 | 1.0 | 1.4 | 1.7 |
Intensity Change (Log2) | – | – | – | 0.07 | 0.04 | 0.06 | -0.37 | -0.1 | -0.12 | -0.35 | 0.26 | -0.09 | 0.48 | 0.36 | 0.36 | 0.52 | 0.84 | 0.85 | 0.23 | 0.34 | 0.61 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | 6.1 | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.6931 | 3.2058 | 3.5156 | 2.9962 | 2.2795 | 2.5632 | 1.007 | 0.7629 | 1.0961 | 1.275 | 0.7051 | 0.752 | 2.0337 | 1.7703 | 1.5879 | 1.8676 | 1.8055 | 1.6372 |
Actin | 0.0632 | 0.002 | 0.0134 | 0.0111 | 0.0043 | 0.0018 | 0.0226 | 0.0001 | 0.0046 | 0.0061 | 0.0018 | 0.009 | 0.0247 | 0.0179 | 0.0046 | 0.0058 | 0.0181 | 0.008 |
Bud | 0.0013 | 0.001 | 0.0005 | 0.0009 | 0.0014 | 0.0001 | 0.0008 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.001 | 0.0077 | 0.0008 | 0.0008 | 0.0002 | 0.0004 |
Bud Neck | 0.0012 | 0.0005 | 0.0015 | 0.002 | 0.0007 | 0.0013 | 0.0017 | 0.0001 | 0.0017 | 0.0003 | 0.0002 | 0.0016 | 0.0017 | 0.0009 | 0.0004 | 0.0011 | 0.0008 | 0.0008 |
Bud Periphery | 0.0029 | 0.001 | 0.0004 | 0.0015 | 0.0026 | 0.0002 | 0.0022 | 0 | 0.0001 | 0.0006 | 0.0001 | 0.0006 | 0.0017 | 0.012 | 0.0006 | 0.0008 | 0.0002 | 0.0009 |
Bud Site | 0.0038 | 0.0267 | 0.0051 | 0.0026 | 0.0004 | 0.0001 | 0.0039 | 0.0004 | 0.0064 | 0.0005 | 0.0001 | 0.0003 | 0.0073 | 0.0124 | 0.0102 | 0.0082 | 0.0005 | 0.0001 |
Cell Periphery | 0.0005 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0007 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0 |
Cytoplasm | 0.1586 | 0.2833 | 0.1886 | 0.322 | 0.3062 | 0.3375 | 0.1155 | 0.1328 | 0.1211 | 0.2002 | 0.2041 | 0.1765 | 0.1698 | 0.2968 | 0.3146 | 0.3021 | 0.2531 | 0.3024 |
Cytoplasmic Foci | 0.0295 | 0.0078 | 0.0111 | 0.0349 | 0.0317 | 0.0173 | 0.0346 | 0.0021 | 0.0283 | 0.0277 | 0.0097 | 0.0133 | 0.0257 | 0.0099 | 0.0076 | 0.0251 | 0.0074 | 0.0096 |
Eisosomes | 0.0008 | 0.0003 | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0001 |
Endoplasmic Reticulum | 0.0138 | 0.0024 | 0.0028 | 0.0049 | 0.0036 | 0.0035 | 0.0116 | 0.001 | 0.0046 | 0.0047 | 0.0012 | 0.0023 | 0.0146 | 0.0023 | 0.0038 | 0.0073 | 0.0033 | 0.0022 |
Endosome | 0.042 | 0.002 | 0.0038 | 0.0275 | 0.0053 | 0.0057 | 0.0636 | 0.0005 | 0.0167 | 0.081 | 0.0115 | 0.0069 | 0.0371 | 0.0021 | 0.0022 | 0.0065 | 0.0073 | 0.002 |
Golgi | 0.0139 | 0.0004 | 0.0025 | 0.0168 | 0.0011 | 0.0011 | 0.0458 | 0 | 0.0061 | 0.041 | 0.0203 | 0.005 | 0.0166 | 0.0012 | 0.0009 | 0.0015 | 0.0149 | 0.0004 |
Lipid Particles | 0.0226 | 0.0008 | 0.0091 | 0.008 | 0.0017 | 0.006 | 0.0284 | 0.0002 | 0.0147 | 0.0056 | 0.001 | 0.002 | 0.0383 | 0.0022 | 0.001 | 0.0016 | 0.0041 | 0.0006 |
Mitochondria | 0.0455 | 0.0005 | 0.0015 | 0.0212 | 0.0011 | 0.0017 | 0.0134 | 0.0002 | 0.0018 | 0.0176 | 0.0425 | 0.0095 | 0.0214 | 0.0023 | 0.0004 | 0.0014 | 0.0203 | 0.0003 |
None | 0.492 | 0.6254 | 0.5596 | 0.4206 | 0.5534 | 0.5136 | 0.5972 | 0.821 | 0.7256 | 0.5814 | 0.6819 | 0.6967 | 0.5172 | 0.5663 | 0.5864 | 0.4876 | 0.6138 | 0.6217 |
Nuclear Periphery | 0.0181 | 0.0023 | 0.0059 | 0.0073 | 0.0038 | 0.0057 | 0.019 | 0.0011 | 0.0056 | 0.0046 | 0.0019 | 0.0037 | 0.0179 | 0.003 | 0.0039 | 0.0161 | 0.0036 | 0.0022 |
Nucleolus | 0.002 | 0.0007 | 0.0045 | 0.0013 | 0.001 | 0.001 | 0.0027 | 0.0006 | 0.0004 | 0.0006 | 0.0002 | 0.0009 | 0.0024 | 0.0006 | 0.0004 | 0.001 | 0.0005 | 0.0002 |
Nucleus | 0.0316 | 0.0256 | 0.1729 | 0.0913 | 0.0439 | 0.0638 | 0.0105 | 0.0355 | 0.0243 | 0.0179 | 0.0163 | 0.0606 | 0.0398 | 0.0326 | 0.0455 | 0.1093 | 0.0437 | 0.0388 |
Peroxisomes | 0.0208 | 0.002 | 0.0026 | 0.0033 | 0.0059 | 0.007 | 0.0098 | 0 | 0.0334 | 0.0027 | 0.0008 | 0.0019 | 0.028 | 0.0102 | 0.0078 | 0.0063 | 0.0018 | 0.0014 |
Punctate Nuclear | 0.0247 | 0.0138 | 0.0117 | 0.017 | 0.0303 | 0.0312 | 0.0061 | 0.0036 | 0.003 | 0.0034 | 0.0044 | 0.007 | 0.0151 | 0.0175 | 0.0077 | 0.015 | 0.0042 | 0.0073 |
Vacuole | 0.007 | 0.0011 | 0.0015 | 0.0039 | 0.001 | 0.001 | 0.0067 | 0.0006 | 0.0009 | 0.0024 | 0.0011 | 0.0014 | 0.0115 | 0.0014 | 0.0008 | 0.0016 | 0.0013 | 0.0005 |
Vacuole Periphery | 0.0043 | 0.0001 | 0.0003 | 0.0015 | 0.0003 | 0.0003 | 0.003 | 0 | 0.0004 | 0.0009 | 0.0005 | 0.0003 | 0.0072 | 0.0002 | 0.0002 | 0.0005 | 0.0005 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 35.9505 | 40.79 | 29.8093 | 40.5593 | 38.5441 | 44.9133 | 48.8976 | 54.6177 | 49.865 | 45.6876 |
Translational Efficiency | 1.0476 | 1.4186 | 1.2952 | 0.9715 | 0.843 | 1.1567 | 1.2325 | 1.1779 | 1.1733 | 1.0598 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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