Standard name
Human Ortholog
Description Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.1 0.1 0.1 0.06 0 0.05 0.08 0.09 0.05 0.1 0 0.06 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0.05 0 0 0 0 0 0 0 0 0.05 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0.07 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0.68 0.82 0.6 0.8 0.56 0.5 0.42 0.39 0.38 0.36 0.35 0.45 – 0.74 0.72 0.71 0.78 0.76 0.74 0.72 0.67 0.43 0.33 0.49
Cytoplasm 0.68 0.41 0.58 0.49 0.51 0.58 0.61 0.66 0.64 0.63 0.57 0.59 – 0.57 0.66 0.56 0.48 0.47 0.13 0.12 0.09 0.29 0.42 0.24
Endoplasmic Reticulum 0 0 0.05 0 0.07 0.12 0.1 0.06 0.06 0.05 0 0 – 0 0.05 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0.08 0.09 0.19 0.15 0.38 0.33 – 0 0 0 0 0 0 0 0 0 0.08 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.05 0.05 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 34 34 13 6 6 10 20 21 8 17 5 11 – 0 2 6 9 13 7 10 4 1 0 0
Bud 16 12 4 1 4 3 8 5 3 9 4 2 – 0 0 9 10 8 0 5 1 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 12 25 10 1 1 1
Bud Site 1 1 0 1 1 0 1 1 0 0 0 0 – 0 0 1 0 0 – – – – – –
Cell Periphery 235 290 77 84 78 101 100 90 66 64 66 86 – 17 47 285 353 332 272 298 98 16 9 26
Cytoplasm 236 143 74 51 71 117 145 154 112 111 106 114 – 13 43 225 218 208 49 51 13 11 12 13
Endoplasmic Reticulum 10 12 7 4 10 24 23 14 10 8 7 7 – 1 3 4 6 11 11 10 4 1 0 1
Endosome 1 0 0 0 0 0 1 1 0 0 0 0 – 0 0 1 0 0 1 1 1 0 0 0
Golgi 1 1 0 0 0 0 0 0 0 0 0 0 – 0 0 2 1 2 2 1 0 0 0 0
Mitochondria 3 1 0 1 0 18 19 21 33 27 71 64 – 0 0 1 1 1 3 1 5 0 2 2
Nucleus 0 4 3 0 2 3 3 1 6 4 3 2 – 1 2 12 9 10 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 7 1 0 0 1 1 1 1 0 0 0 0 – 0 0 1 1 2 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 1 6 10 11 4 3 7 0 3 – 0 0 3 8 8 2 4 1 1 0 0
Unique Cell Count 346 353 128 105 140 202 236 232 176 177 187 193 23 65 402 452 438 368 415 146 38 29 55
Labelled Cell Count 544 499 179 149 180 287 332 313 241 247 263 289 32 97 550 616 595 368 415 146 38 29 55


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.6 14.0 9.9 10.1 10.1 8.6 8.3 8.4 7.0 7.1 6.3 6.7 – 11.8 12.3 16.4 16.5 15.4 – – 13.2
Std Deviation (1e-4) 1.9 2.7 1.5 1.4 1.5 1.5 1.7 1.5 1.2 1.5 1.3 1.4 – 2.1 2.5 3.5 3.3 3.3 – – 2.6
Intensity Change (Log2) – – – 0.03 0.03 -0.21 -0.25 -0.24 -0.49 -0.48 -0.66 -0.57 – 0.25 0.31 0.73 0.73 0.64 – – 0.41


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.2 -1.9 -1.8 -0.5 -0.3 -1.9 -0.2 -2.8 -1.5 – 0 -1.7 0 0 -3.4
Bud 0 0 0 0 0 0 0.8 0 0 – 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Cell Periphery 3.3 -0.7 -1.8 -3.2 -3.9 -3.9 -4.1 -4.4 -2.7 – 1.3 1.7 2.3 4.1 3.5
Cytoplasm -1.4 -1.2 0 0.7 1.6 1.0 0.9 -0.2 0.2 – -0.1 1.1 -0.4 -1.9 -2.1
Endoplasmic Reticulum 0 0.6 1.9 1.4 0.2 0.1 -0.4 -0.7 -0.8 – 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Mitochondria 0 0 3.5 3.3 3.5 5.2 4.6 7.9 7.3 – 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.0367 9.5298 9.4715 8.5829 8.8513 8.9867 8.7211 11.1045 10.0488 9.931 9.3813 9.7398 9.1359 11.2078 10.1635 9.2047 8.2718 10.6155
Actin 0.0311 0.0036 0.0098 0.0232 0.0024 0.0049 0.0221 0.0047 0.0208 0.0443 0.0316 0.0136 0.0465 0.0071 0.0175 0.0312 0.0604 0.0091
Bud 0.0012 0.0102 0.0109 0.0259 0.0082 0.0064 0.0033 0.0012 0.0019 0.0045 0.001 0.0011 0.0014 0.0004 0.0018 0.0011 0.0008 0.0011
Bud Neck 0.0054 0.0022 0.0056 0.0045 0.0046 0.0211 0.0032 0.0031 0.014 0.0171 0.0034 0.0308 0.0038 0.0016 0.0057 0.0028 0.0049 0.0326
Bud Periphery 0.0069 0.0064 0.0272 0.0369 0.0179 0.0157 0.0197 0.0049 0.0158 0.0187 0.0161 0.0132 0.0122 0.0019 0.0176 0.0224 0.0069 0.0119
Bud Site 0.0157 0.0761 0.0599 0.0485 0.0054 0.0054 0.0139 0.0727 0.0335 0.0145 0.0022 0.0014 0.0052 0.0578 0.027 0.0124 0.0041 0.0021
Cell Periphery 0.4557 0.2612 0.4016 0.2891 0.3441 0.5095 0.5365 0.4513 0.5014 0.405 0.6037 0.5895 0.5999 0.5004 0.5864 0.5385 0.4119 0.6306
Cytoplasm 0.3731 0.6299 0.4493 0.5319 0.545 0.4015 0.2785 0.4434 0.3554 0.3945 0.2462 0.3125 0.2094 0.4179 0.2939 0.3218 0.3422 0.2835
Cytoplasmic Foci 0.0129 0.0004 0.0011 0.0013 0.0008 0.001 0.0083 0.0013 0.0025 0.0181 0.004 0.0012 0.0079 0.0004 0.0023 0.0027 0.0093 0.0004
Eisosomes 0.0035 0.0003 0.0003 0.0013 0.0002 0.0011 0.0041 0.0004 0.0014 0.0005 0.0005 0.0008 0.008 0.0001 0.0011 0.0005 0.0216 0.0001
Endoplasmic Reticulum 0.0124 0.0054 0.0075 0.006 0.0121 0.0164 0.0294 0.0066 0.0137 0.0257 0.0225 0.0144 0.0276 0.0064 0.0102 0.038 0.0186 0.0111
Endosome 0.0144 0.0001 0.0026 0.0019 0.0155 0.0003 0.0115 0.0006 0.0053 0.0124 0.0115 0.004 0.012 0.0001 0.0028 0.0015 0.0102 0.0024
Golgi 0.0063 0.0004 0.0029 0.0033 0.0012 0.0007 0.0079 0.0008 0.0031 0.0163 0.006 0.0052 0.0077 0.0005 0.0024 0.0016 0.0077 0.0011
Lipid Particles 0.0083 0.0006 0.0012 0.0009 0.0011 0.001 0.011 0.0016 0.0047 0.0063 0.0072 0.0028 0.0141 0.0007 0.0049 0.0021 0.0107 0.0011
Mitochondria 0.0026 0.0001 0.0039 0.0161 0.0038 0.0015 0.0079 0.0008 0.0038 0.0061 0.0193 0.0019 0.0036 0.0002 0.0046 0.0013 0.0057 0.0004
None 0.0185 0.0004 0.006 0.0011 0.0029 0.0053 0.0043 0.0008 0.0034 0.001 0.0013 0.0003 0.0024 0.0001 0.0009 0.0017 0.0174 0.0002
Nuclear Periphery 0.0052 0.0004 0.0025 0.0005 0.0062 0.0004 0.0084 0.0007 0.004 0.0017 0.006 0.001 0.0079 0.0005 0.0024 0.0022 0.0125 0.0056
Nucleolus 0.0009 0.0001 0.0004 0.0003 0.0017 0.0004 0.0009 0.0003 0.0006 0.0003 0.0004 0.0002 0.0006 0 0.0004 0.0005 0.0023 0.0001
Nucleus 0.0061 0.0004 0.0018 0.001 0.0118 0.0009 0.0083 0.0006 0.0043 0.0013 0.0011 0.0005 0.0023 0.0002 0.0007 0.0031 0.0039 0.0005
Peroxisomes 0.0111 0 0.0005 0.0004 0.0001 0.0001 0.0065 0.0018 0.0024 0.0054 0.0015 0.0007 0.0155 0.0014 0.0099 0.0017 0.0051 0.0002
Punctate Nuclear 0.0016 0 0.0005 0.0001 0.0007 0.0002 0.003 0.0001 0.0008 0.0011 0.0023 0.0004 0.0051 0 0.0019 0.0041 0.0404 0.0004
Vacuole 0.0066 0.0017 0.0041 0.0056 0.0126 0.0061 0.01 0.0025 0.0066 0.0048 0.0105 0.0041 0.0064 0.0025 0.0056 0.0086 0.0026 0.0053
Vacuole Periphery 0.0007 0 0.0003 0.0003 0.0019 0.0001 0.0014 0 0.0005 0.0005 0.0017 0.0004 0.0007 0 0.0002 0.0001 0.0008 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.6223 7.9418 5.8932 17.5861 11.9026 17.7584 17.3983 22.0401 20.2748 20.7192
Translational Efficiency 0.8098 1.1006 1.5447 0.6407 0.7522 0.9593 0.7634 0.5856 0.9008 0.8392

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (34%), cell periphery (5%), mixed (61%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Rtk1

Rtk1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rtk1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available