Standard name
Human Ortholog
Description Subunit of the SF3a splicing factor complex; required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0.05 0 0 0.05 0.07 0 0.11 0.13 0.18 0.08 0.07 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.17 0.14 0.43 0.43 0.37 0.67 0.7 0.54 0.69 0.79 0.72 0.8 0.05 0 0 0 0.07 0.1 0 0 0 0 0 0
Nucleus 0.96 0.93 0.89 0.88 0.87 0.77 0.69 0.78 0.72 0.71 0.72 0.68 0.88 0.83 0.78 0.87 0.82 0.81 0.88 0.88 0.87 0.83 0.77 0.66
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0 0.05 0.07 0.06 0.1 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1 0 0 3 3 7 4 0 1 0 0 0 0 0 0 0 4 0 1 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 12 8 12 8 6 12 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 3 4 8 14 11 23 24 16 18 13 5 19 50 65 7 3 1 0 1 1 1 1 1
Endoplasmic Reticulum 0 1 0 0 1 1 1 0 0 0 0 0 0 2 0 3 1 1 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 3 3 0 1 0 1 2 0 1 1 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 2 1 2
Mitochondria 27 43 84 79 106 220 334 319 291 307 141 127 9 8 6 3 3 2 0 3 3 0 4 4
Nucleus 149 285 175 161 249 252 329 461 302 273 141 107 150 312 283 72 33 17 138 273 169 145 155 124
Nuclear Periphery 1 1 0 4 3 7 9 7 6 6 1 4 1 1 2 0 0 0 0 0 0 2 0 2
Nucleolus 9 7 1 0 2 4 7 17 14 5 7 5 3 6 1 0 0 0 6 3 0 3 10 8
Peroxisomes 0 1 0 0 0 0 0 0 0 0 0 0 0 2 4 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 1 0 0 1 6 5 1 0 0 1 0 3 5 1 0 1 0 2 1 1 1 0
Vac/Vac Membrane 1 9 1 2 6 2 9 3 2 1 0 4 1 7 9 5 0 1 6 16 14 11 20 35
Unique Cell Count 156 305 196 184 285 327 477 588 421 387 195 158 171 377 364 83 40 21 158 312 195 175 202 189
Labelled Cell Count 191 351 266 255 381 510 729 851 647 620 315 259 183 394 378 91 41 23 158 312 195 175 202 189


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 5.1 4.2 4.0 4.2 3.7 3.2 3.7 3.5 3.2 3.5 3.2 5.7 5.8 5.7 6.8 7.2 8.1 5.5 5.7 6.2
Std Deviation (1e-4) 0.8 0.9 0.7 1.0 1.2 1.0 1.2 1.0 0.9 1.4 1.4 1.0 1.3 1.6 1.4 2.0 1.6 1.6 1.4 1.3 1.1
Intensity Change (Log2) – – – -0.07 -0.03 -0.21 -0.39 -0.19 -0.28 -0.4 -0.28 -0.4 0.41 0.46 0.42 0.68 0.77 0.93 0.38 0.43 0.55


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.3 1.6 0.9 1.7 1.3 1.1 1.6 2.2 0 3.6 4.4 5.4 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -8.3 -12.6 -12.7 -6.5 -4.2 -3.0
Nucleus -0.5 -0.6 -3.5 -5.5 -3.4 -4.8 -5.1 -4.3 -5.0 -0.5 -2.1 -3.4 -0.6 -1.2 -1.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.9694 4.5751 4.478 4.334 4.5563 4.3711 2.7288 2.9741 2.4119 2.0286 1.9265 2.2314 2.5726 3.2757 3.3368 3.6512 3.3267 3.4674
Actin 0.0621 0.0006 0.0066 0.001 0.0003 0.0037 0.0303 0 0.019 0 0.0029 0.0015 0 0 0.045 0.0001 0.006 0.0047
Bud 0.0012 0.0002 0.0003 0.0006 0.0001 0.0002 0.0008 0.0001 0.0006 0 0.0017 0 0 0 0.0001 0.0002 0.0049 0.0003
Bud Neck 0.0251 0.0023 0.0008 0.001 0.0006 0.0007 0.0018 0.0002 0.0014 0 0.0004 0.0005 0 0.0001 0.0001 0.0003 0.0008 0.0006
Bud Periphery 0.0035 0.0004 0.001 0.002 0.0001 0.0005 0.0014 0.0002 0.0009 0 0.0036 0 0 0 0.0001 0.0006 0.0059 0.0012
Bud Site 0.0108 0.0093 0.0013 0.0005 0.0005 0.0004 0.0055 0.0024 0.0063 0 0.0011 0.0001 0 0 0.0002 0.0001 0.0065 0.0003
Cell Periphery 0.0015 0.0007 0.0003 0.0022 0 0.0001 0.0006 0.0001 0.0003 0 0.0002 0 0 0 0 0.0001 0.0002 0.0004
Cytoplasm 0.0287 0.0001 0.0004 0.0029 0.0005 0.0006 0.008 0.0006 0.001 0.0019 0.0015 0.0005 0 0.0004 0.0002 0.0002 0.0148 0.001
Cytoplasmic Foci 0.0076 0.0009 0.005 0.0004 0.0178 0.0021 0.0429 0.0027 0.0261 0 0.0078 0.0067 0 0 0.0022 0 0.0334 0.0002
Eisosomes 0.0004 0.0001 0.0002 0 0 0 0.0004 0 0.0001 0 0 0 0 0 0.0001 0 0.0001 0.0001
Endoplasmic Reticulum 0.0088 0.0001 0.0013 0.0018 0.0001 0.0001 0.004 0 0.0004 0 0.0014 0.0001 0 0 0 0.0001 0.0039 0.0042
Endosome 0.0311 0.0011 0.0109 0.0117 0.0071 0.0004 0.0682 0.0001 0.0038 0 0.0141 0.008 0 0 0.001 0.0001 0.0891 0.0015
Golgi 0.0125 0.0006 0.0058 0.0074 0.0022 0.0007 0.0279 0.0001 0.004 0 0.0056 0.0031 0 0 0.0096 0 0.0135 0.0014
Lipid Particles 0.0197 0.0034 0.0176 0.001 0.0099 0.001 0.0406 0.0022 0.0168 0 0.0332 0.0026 0 0 0.0004 0 0.0061 0.0001
Mitochondria 0.0167 0.005 0.0237 0.039 0.0009 0.0022 0.0155 0.0004 0.0073 0 0.0136 0.0024 0.0001 0.0003 0.0017 0.0013 0.0407 0.0024
None 0.0015 0.0001 0.0008 0.0006 0.0004 0.0001 0.0251 0.0007 0.0004 0.001 0.0003 0.0001 0 0.0001 0 0.0001 0.0028 0.0001
Nuclear Periphery 0.0405 0.0011 0.0094 0.009 0.0011 0.0014 0.0111 0.0025 0.0038 0.0024 0.0019 0.0002 0.0001 0.0021 0.0016 0.002 0.02 0.0006
Nucleolus 0.0096 0.0126 0.008 0.0232 0.0188 0.0193 0.0225 0.0119 0.0047 0.004 0.0264 0.0155 0.0054 0.0076 0.0035 0.0169 0.0119 0.012
Nucleus 0.6942 0.9566 0.8883 0.8628 0.9231 0.9614 0.6386 0.9721 0.8802 0.9835 0.8385 0.9511 0.9943 0.9888 0.9319 0.9758 0.6983 0.9681
Peroxisomes 0.0048 0.0019 0.0114 0.0002 0.0089 0.0024 0.0217 0.0016 0.0202 0 0.0118 0.0064 0 0 0.0023 0 0.0133 0
Punctate Nuclear 0.0051 0.0012 0.0032 0.0002 0.0072 0.0023 0.0242 0.0019 0.001 0.007 0.0301 0.0011 0 0.0003 0.0002 0.0001 0.0111 0.0002
Vacuole 0.0096 0.0015 0.002 0.021 0.0003 0.0003 0.0068 0.0002 0.0013 0 0.0026 0.0001 0 0.0001 0 0.001 0.0099 0.0003
Vacuole Periphery 0.0051 0.0002 0.0015 0.0112 0.0001 0.0001 0.0024 0.0001 0.0004 0 0.0013 0.0001 0 0.0001 0 0.0009 0.0067 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.4289 8.0495 14.703 10.3612 10.3277 9.6918 11.7387 10.5638 12.021 12.005
Translational Efficiency 1.2452 1.5952 0.7229 1.026 1.1683 1.1424 0.9911 0.9901 0.8795 1.1834

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of the SF3a splicing factor complex; required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Prp9

Prp9


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Prp9-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available