Standard name | |
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Human Ortholog | |
Description | tRNA-specific 2-thiouridylase; responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human homolog TRMU is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF), and can complement yeast null mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 22 | 4 | 5 | 1 | 0 | 1 | 3 | 0 | 2 | 3 | 10 | 14 |
Bud | 14 | 4 | 9 | 11 | 12 | 13 | 29 | 24 | 21 | 27 | 29 | 17 | 22 | 12 | 8 | 8 | 4 | 14 | 0 | 2 | 0 | 2 | 7 | 10 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 6 | 6 | 6 | 0 | 1 | 0 | 1 | 2 | 1 | 4 | 10 | 17 |
Bud Site | 1 | 1 | 2 | 3 | 0 | 6 | 23 | 17 | 6 | 13 | 10 | 10 | 1 | 0 | 2 | 2 | 3 | 3 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 6 | 3 | 3 | 4 | 3 | 3 | 6 | 2 | 1 | 6 | 16 | 5 | 3 | 8 | 9 | 10 | 0 | 0 | 0 | 0 | 1 | 1 |
Cytoplasm | 7 | 26 | 28 | 10 | 23 | 15 | 19 | 13 | 7 | 12 | 11 | 6 | 112 | 138 | 196 | 16 | 27 | 19 | 1 | 5 | 1 | 12 | 21 | 18 |
Endoplasmic Reticulum | 6 | 1 | 2 | 1 | 2 | 1 | 2 | 1 | 3 | 3 | 1 | 7 | 1 | 3 | 10 | 3 | 9 | 4 | 1 | 0 | 0 | 0 | 3 | 4 |
Endosome | 5 | 12 | 18 | 12 | 46 | 11 | 5 | 29 | 5 | 0 | 0 | 2 | 81 | 116 | 121 | 40 | 19 | 42 | 32 | 18 | 26 | 21 | 48 | 59 |
Golgi | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 6 | 7 | 7 | 9 | 2 | 0 | 6 | 2 | 5 | 4 |
Mitochondria | 178 | 81 | 148 | 194 | 340 | 350 | 653 | 711 | 496 | 715 | 454 | 608 | 151 | 35 | 34 | 22 | 23 | 18 | 149 | 91 | 167 | 123 | 343 | 369 |
Nucleus | 1 | 0 | 1 | 1 | 3 | 11 | 28 | 27 | 10 | 13 | 13 | 4 | 1 | 1 | 1 | 2 | 4 | 3 | 0 | 1 | 0 | 2 | 4 | 8 |
Nuclear Periphery | 2 | 0 | 0 | 0 | 0 | 8 | 17 | 21 | 12 | 19 | 8 | 23 | 0 | 0 | 0 | 1 | 2 | 1 | 1 | 0 | 1 | 0 | 1 | 4 |
Nucleolus | 1 | 1 | 2 | 0 | 4 | 7 | 26 | 15 | 14 | 23 | 8 | 11 | 0 | 1 | 1 | 3 | 2 | 3 | 1 | 1 | 0 | 0 | 3 | 5 |
Peroxisomes | 7 | 9 | 52 | 30 | 64 | 38 | 23 | 34 | 6 | 19 | 2 | 5 | 41 | 33 | 50 | 36 | 32 | 30 | 13 | 2 | 22 | 3 | 15 | 29 |
SpindlePole | 23 | 17 | 32 | 22 | 23 | 33 | 58 | 63 | 21 | 40 | 12 | 21 | 86 | 129 | 144 | 48 | 43 | 39 | 10 | 13 | 8 | 15 | 50 | 44 |
Vac/Vac Membrane | 12 | 14 | 14 | 6 | 34 | 32 | 25 | 36 | 23 | 18 | 18 | 23 | 46 | 72 | 47 | 24 | 21 | 26 | 4 | 10 | 2 | 13 | 52 | 63 |
Unique Cell Count | 206 | 130 | 226 | 218 | 424 | 404 | 726 | 808 | 531 | 749 | 473 | 631 | 472 | 464 | 526 | 156 | 144 | 147 | 229 | 155 | 246 | 215 | 590 | 673 |
Labelled Cell Count | 259 | 168 | 315 | 295 | 554 | 529 | 911 | 995 | 631 | 904 | 567 | 744 | 589 | 558 | 634 | 221 | 206 | 222 | 229 | 155 | 246 | 215 | 590 | 673 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.9 | 4.3 | 4.7 | 4.4 | 4.6 | 4.1 | 4.0 | 4.0 | 3.9 | 3.8 | 3.5 | 3.7 | 5.7 | 5.6 | 5.5 | 6.1 | 6.7 | 6.4 | 5.5 | 5.6 | 6.1 |
Std Deviation (1e-4) | 0.6 | 0.8 | 1.7 | 1.8 | 1.6 | 1.5 | 1.3 | 0.9 | 0.9 | 1.0 | 0.8 | 0.9 | 0.8 | 0.7 | 1.0 | 1.4 | 1.7 | 1.6 | 1.0 | 1.1 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.09 | -0.04 | -0.19 | -0.24 | -0.23 | -0.26 | -0.3 | -0.42 | -0.34 | 0.27 | 0.25 | 0.23 | 0.38 | 0.5 | 0.44 | 0.22 | 0.25 | 0.37 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 2.9 | 2.7 | 3.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.8516 | 2.3013 | 2.3318 | 2.1557 | 2.0501 | 2.3044 | 0.0766 | 0.3533 | 0.142 | 0.0466 | 0.2373 | 0.1172 | 0.2884 | 0.4198 | 0.3066 | 0.3076 | 0.4112 | 0.1276 |
Actin | 0.0481 | 0.045 | 0.0128 | 0.0189 | 0.0065 | 0.0343 | 0.0919 | 0.0254 | 0.0328 | 0.0334 | 0.0053 | 0.0219 | 0.0289 | 0.0174 | 0.0326 | 0.0223 | 0.0793 | 0.0105 |
Bud | 0.0003 | 0.0018 | 0.0018 | 0.0023 | 0.0072 | 0.0007 | 0.0018 | 0.0019 | 0.0033 | 0.0071 | 0.0013 | 0.001 | 0.0009 | 0.0019 | 0.0061 | 0.0051 | 0.0219 | 0.001 |
Bud Neck | 0.0004 | 0.0013 | 0.0063 | 0.0152 | 0.0004 | 0.0023 | 0.0059 | 0.0033 | 0.0008 | 0.0011 | 0.0002 | 0.0023 | 0.0043 | 0.0024 | 0.002 | 0.001 | 0.0029 | 0.0008 |
Bud Periphery | 0.0007 | 0.003 | 0.0052 | 0.0135 | 0.026 | 0.002 | 0.003 | 0.0027 | 0.004 | 0.0273 | 0.0077 | 0.0024 | 0.0021 | 0.0026 | 0.0084 | 0.0053 | 0.0236 | 0.0017 |
Bud Site | 0.0013 | 0.0401 | 0.022 | 0.0097 | 0.0059 | 0.0016 | 0.0283 | 0.0239 | 0.0096 | 0.0054 | 0.0003 | 0.0049 | 0.0056 | 0.0268 | 0.0172 | 0.0129 | 0.0091 | 0.0008 |
Cell Periphery | 0.0003 | 0.0014 | 0.0029 | 0.0494 | 0.0007 | 0.0015 | 0.0013 | 0.001 | 0.0012 | 0.0061 | 0.0003 | 0.001 | 0.0008 | 0.0016 | 0.0019 | 0.0005 | 0.001 | 0.0006 |
Cytoplasm | 0.0009 | 0.0024 | 0.0052 | 0.0052 | 0.0021 | 0.003 | 0.0021 | 0.0062 | 0.0115 | 0.0037 | 0.0009 | 0.0132 | 0.0056 | 0.0044 | 0.0038 | 0.002 | 0.0097 | 0.0015 |
Cytoplasmic Foci | 0.0152 | 0.0264 | 0.041 | 0.0212 | 0.0174 | 0.0281 | 0.0298 | 0.0431 | 0.0422 | 0.0169 | 0.0082 | 0.0096 | 0.0325 | 0.0491 | 0.034 | 0.0238 | 0.0479 | 0.0289 |
Eisosomes | 0.0007 | 0.0006 | 0.0021 | 0.0009 | 0.0008 | 0.0009 | 0.001 | 0.0004 | 0.0007 | 0.0004 | 0.0002 | 0.0009 | 0.0013 | 0.0004 | 0.0005 | 0.0003 | 0.0005 | 0.0004 |
Endoplasmic Reticulum | 0.0018 | 0.0034 | 0.0018 | 0.0009 | 0.0005 | 0.0025 | 0.0028 | 0.0007 | 0.0019 | 0.0005 | 0.0018 | 0.0065 | 0.0044 | 0.0013 | 0.0014 | 0.0014 | 0.0017 | 0.0013 |
Endosome | 0.071 | 0.0446 | 0.0398 | 0.0972 | 0.0884 | 0.0508 | 0.0593 | 0.0732 | 0.0619 | 0.0277 | 0.0874 | 0.0416 | 0.0975 | 0.0771 | 0.0872 | 0.0503 | 0.1003 | 0.05 |
Golgi | 0.0399 | 0.0655 | 0.0301 | 0.0434 | 0.0349 | 0.0489 | 0.0563 | 0.0643 | 0.0775 | 0.0531 | 0.1115 | 0.0415 | 0.0464 | 0.0552 | 0.0352 | 0.0338 | 0.107 | 0.0373 |
Lipid Particles | 0.0543 | 0.0269 | 0.0208 | 0.0147 | 0.0052 | 0.0211 | 0.0449 | 0.0317 | 0.0181 | 0.015 | 0.0375 | 0.0169 | 0.0946 | 0.0544 | 0.0178 | 0.0213 | 0.0183 | 0.0376 |
Mitochondria | 0.6962 | 0.6372 | 0.7354 | 0.6417 | 0.7179 | 0.7138 | 0.6 | 0.6507 | 0.6631 | 0.7754 | 0.7263 | 0.8029 | 0.5586 | 0.5919 | 0.6874 | 0.7924 | 0.4826 | 0.769 |
None | 0.0009 | 0.0006 | 0.0093 | 0.0019 | 0.0012 | 0.0277 | 0.0014 | 0.0006 | 0.0014 | 0.0007 | 0.0004 | 0.0013 | 0.0037 | 0.0093 | 0.0099 | 0.001 | 0.0067 | 0.0013 |
Nuclear Periphery | 0.0109 | 0.0007 | 0.0005 | 0.0004 | 0.0003 | 0.0019 | 0.006 | 0.0004 | 0.0019 | 0.0003 | 0.0011 | 0.0049 | 0.0104 | 0.0015 | 0.0019 | 0.0005 | 0.0066 | 0.0023 |
Nucleolus | 0.0004 | 0.0005 | 0.0008 | 0.0011 | 0.0004 | 0.0005 | 0.001 | 0.0025 | 0.0004 | 0.0003 | 0.0001 | 0.0005 | 0.0046 | 0.0036 | 0.0023 | 0.0009 | 0.0015 | 0.0008 |
Nucleus | 0.0007 | 0.0005 | 0.0006 | 0.0011 | 0.0005 | 0.0004 | 0.0154 | 0.0011 | 0.0008 | 0.0005 | 0.0002 | 0.0009 | 0.0276 | 0.0013 | 0.0025 | 0.0006 | 0.0033 | 0.0006 |
Peroxisomes | 0.0348 | 0.0891 | 0.0424 | 0.0306 | 0.065 | 0.0459 | 0.0345 | 0.0356 | 0.0575 | 0.018 | 0.0023 | 0.0148 | 0.0365 | 0.0676 | 0.0256 | 0.0144 | 0.0456 | 0.0398 |
Punctate Nuclear | 0.0003 | 0.0002 | 0.0004 | 0.0003 | 0.0001 | 0.0013 | 0.0015 | 0.0003 | 0.0046 | 0.0002 | 0.0003 | 0.0017 | 0.0034 | 0.0005 | 0.0025 | 0.0002 | 0.006 | 0.0016 |
Vacuole | 0.0086 | 0.0056 | 0.0133 | 0.0218 | 0.0109 | 0.006 | 0.0061 | 0.0212 | 0.0024 | 0.0034 | 0.0022 | 0.0036 | 0.0192 | 0.0166 | 0.0126 | 0.0042 | 0.0135 | 0.0045 |
Vacuole Periphery | 0.0122 | 0.0031 | 0.0054 | 0.0083 | 0.0078 | 0.0048 | 0.0058 | 0.0099 | 0.0024 | 0.0033 | 0.0046 | 0.0057 | 0.0111 | 0.013 | 0.0072 | 0.0058 | 0.011 | 0.0077 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 14.5185 | 11.7988 | 8.1715 | 9.3281 | 12.5492 | 8.7942 | 11.9297 | 10.7357 | 14.4301 | 12.4903 |
Translational Efficiency | 0.373 | 0.4175 | 0.5416 | 0.3759 | 0.4385 | 0.5068 | 0.5455 | 0.5361 | 0.2737 | 0.5042 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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