Standard name
Human Ortholog
Description Subunit of protein N-terminal acetyltransferase NatA; NatA comprised of Nat1p, Ard1p, and Nat5p; N-terminally acetylates many proteins to influence multiple processes such as cell cycle progression, heat-shock resistance, mating, sporulation, telomeric silencing and early stages of mitophagy; orthologous to human NAA15; expression of both human NAA10 and NAA15 functionally complements ard1 nat1 double mutant although single mutations are not complemented by their orthologs
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0.05 0.05 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0.06 0.06 0.07 0.06 0.06 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.97 0.98 0.95 0.91 0.83 0.74 0.73 0.73 0.7 0.66 0.93 0.97 0.97 0.96 0.94 0.9 0.9 0.97 0.94 0.79 0.78 0.79
Endoplasmic Reticulum 0 0 0.05 0 0 0.07 0.08 0.14 0.09 0.1 0.05 0.21 0.07 0 0 0 0 0 0 0 0 0.12 0.14 0.13
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.06 0 0.19 0.14 0.3 0.14 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.06 0 0.06 0 0 0 0 0 0 0 0.08 0 0 0 0.05 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 2 18 30 17 11 12 13 0 0 0 2 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 5 4 3 2 5 2 0 1 0 0 1 0 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 4 2 2 2 5 18 38 24 22 18 15 5 3 3 19 11 11 0 0 0 0 0 0
Cytoplasm 121 166 254 180 237 326 449 487 274 245 198 174 326 367 448 365 268 189 114 170 261 169 276 331
Endoplasmic Reticulum 0 3 12 4 9 25 41 89 34 35 15 55 25 14 13 13 11 6 2 1 5 26 49 53
Endosome 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Golgi 0 0 0 0 2 0 7 18 0 2 0 2 0 0 0 0 0 1 0 0 1 0 1 2
Mitochondria 1 0 0 0 1 9 31 23 70 46 85 38 1 0 0 1 3 0 3 0 2 2 1 4
Nucleus 1 1 1 1 3 0 0 6 2 3 2 2 1 2 1 0 0 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 3 0 5 3 15 42 8 20 8 6 1 2 2 15 9 16 2 1 6 10 16 15
Unique Cell Count 122 170 263 183 249 357 539 658 375 337 281 263 350 380 463 382 284 209 127 176 280 214 355 417
Labelled Cell Count 123 175 272 188 259 371 585 740 432 386 343 307 359 389 467 415 303 224 127 176 280 214 355 417


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 19.2 20.5 16.7 15.9 14.9 12.4 12.2 12.3 10.2 9.9 8.6 9.5 22.3 22.7 21.9 25.2 24.6 23.7 19.4 19.9 21.7
Std Deviation (1e-4) 4.8 3.4 2.6 2.8 2.5 1.9 1.9 2.0 2.1 1.8 1.6 1.8 3.2 3.7 3.6 8.0 8.0 8.2 2.7 3.7 4.0
Intensity Change (Log2) – – – -0.07 -0.17 -0.43 -0.46 -0.45 -0.71 -0.76 -0.97 -0.81 0.41 0.44 0.38 0.59 0.56 0.5 0.21 0.25 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 3.0 3.5 3.5 0 3.4 3.7 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 2.2 3.4 3.5 3.6 3.5 3.2 0 0 0 3.0 2.4 3.0
Cytoplasm 1.1 -0.8 -2.6 -5.4 -7.8 -7.7 -7.8 -8.1 -9.0 -1.9 0 0.1 -0.7 -1.2 -2.8
Endoplasmic Reticulum -1.3 -0.5 1.3 1.6 3.9 2.2 2.6 0.4 5.6 1.3 -0.6 -1.2 -0.7 -0.4 -1.0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 2.7 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 4.0 3.1 7.4 6.2 9.7 6.4 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 1.5 3.3 0 3.0 1.4 0 0 0 0 2.1 1.6 3.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 14.0184 21.0674 18.7755 17.758 13.6344 18.8167 16.3364 22.3955 20.8474 19.5396 18.6088 20.4347 17.0733 23.0115 21.4769 18.7621 19.9496 20.7261
Actin 0.0255 0.0016 0.0112 0.001 0.0493 0.0012 0.0095 0.0015 0.007 0.0004 0.0001 0.001 0.0123 0.0003 0.0081 0.0269 0.0002 0.0013
Bud 0.0018 0.001 0.0018 0.0025 0.001 0.0013 0.0007 0.0005 0.0009 0.0008 0.0003 0.0004 0.0006 0.0004 0.0009 0.0007 0.0001 0.0013
Bud Neck 0.0016 0.0006 0.0009 0.0013 0.0012 0.0017 0.0006 0.0002 0.0006 0.0006 0.0003 0.0008 0.0007 0.0002 0.0005 0.0009 0.0004 0.0009
Bud Periphery 0.0034 0.0007 0.0014 0.0015 0.0012 0.0019 0.0004 0.0001 0.0004 0.0002 0 0.0001 0.0004 0.0001 0.0005 0.0004 0 0.002
Bud Site 0.0046 0.0043 0.0014 0.0008 0.0014 0.0004 0.0009 0.0077 0.0028 0.0008 0.0001 0.0001 0.0009 0.001 0.0004 0.0019 0 0.0001
Cell Periphery 0.0017 0.0017 0.0013 0.0019 0.0004 0.0008 0.0004 0.0001 0.0002 0.0001 0 0.0001 0.0007 0.0001 0.0003 0.0002 0 0.0002
Cytoplasm 0.6041 0.8953 0.8389 0.7126 0.5669 0.8102 0.8066 0.9643 0.9149 0.8417 0.915 0.8848 0.8086 0.9634 0.8865 0.7407 0.8698 0.8368
Cytoplasmic Foci 0.0194 0.0046 0.0078 0.0091 0.0475 0.0122 0.0144 0.0049 0.0083 0.0169 0.0116 0.0116 0.0093 0.0058 0.0094 0.0134 0.0115 0.011
Eisosomes 0.0005 0 0.0001 0 0.0009 0 0.0001 0 0.0001 0 0 0 0.0001 0 0 0.0003 0 0
Endoplasmic Reticulum 0.0887 0.0349 0.0282 0.0414 0.0298 0.0486 0.0223 0.0034 0.0037 0.0089 0.0046 0.0116 0.069 0.0062 0.0204 0.0117 0.0258 0.067
Endosome 0.0622 0.0158 0.035 0.0912 0.0938 0.0487 0.0774 0.0093 0.0275 0.0699 0.0453 0.058 0.038 0.0105 0.0342 0.0602 0.0722 0.0461
Golgi 0.0139 0.0022 0.0047 0.0062 0.0199 0.0098 0.0183 0.0008 0.0029 0.0034 0.0008 0.009 0.0063 0.0009 0.0032 0.0062 0.0017 0.0059
Lipid Particles 0.0175 0.0003 0.0007 0.0006 0.0311 0.0004 0.0017 0 0.0002 0.0002 0 0.0003 0.0024 0.0001 0.0013 0.0045 0 0.0001
Mitochondria 0.0377 0.0124 0.014 0.0025 0.0673 0.024 0.0045 0.0003 0.0085 0.0009 0.0004 0.0079 0.002 0.0002 0.0017 0.0031 0.0007 0.0096
None 0.0021 0.0024 0.0007 0.0004 0.0026 0.0009 0.0017 0.0002 0.0003 0.0003 0.0002 0.0003 0.002 0.0003 0.0004 0.0019 0.0003 0.0004
Nuclear Periphery 0.0182 0.003 0.0036 0.0046 0.0075 0.0061 0.0074 0.0008 0.0009 0.0033 0.0017 0.0013 0.0095 0.0007 0.0027 0.0093 0.0017 0.0033
Nucleolus 0.0009 0.0001 0.0001 0.0001 0.0003 0.0025 0.0001 0 0 0 0 0 0.0004 0 0 0.0005 0 0
Nucleus 0.0042 0.0013 0.0017 0.0021 0.0021 0.0049 0.0023 0.0008 0.0009 0.0015 0.0008 0.0007 0.0038 0.0007 0.0013 0.0039 0.0006 0.0011
Peroxisomes 0.0138 0.0001 0.0008 0.0001 0.0345 0.0001 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001 0.0007 0 0.001 0.0008 0 0.0001
Punctate Nuclear 0.0009 0.0001 0.0004 0.0001 0.0035 0.0008 0.0006 0.0001 0.0001 0.0001 0 0 0.0054 0 0.0002 0.0062 0.0001 0.0001
Vacuole 0.0584 0.015 0.0371 0.0957 0.0283 0.0159 0.025 0.0041 0.016 0.0383 0.0138 0.0066 0.0215 0.0079 0.0216 0.0839 0.0097 0.0082
Vacuole Periphery 0.019 0.0027 0.0083 0.0243 0.0096 0.0078 0.0048 0.0009 0.0038 0.0115 0.0048 0.0054 0.0054 0.0012 0.0052 0.0224 0.0049 0.0045

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 97.8653 84.2169 80.1845 86.0489 103.7403 81.2585 81.7495 73.6109 85.0677 98.8686
Translational Efficiency 1.0868 1.064 0.9492 1.0125 0.9179 1.2725 0.9369 0.9666 0.8937 0.9438

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of protein N-terminal acetyltransferase NatA; NatA comprised of Nat1p, Ard1p, and Nat5p; N-terminally acetylates many proteins to influence multiple processes such as cell cycle progression, heat-shock resistance, mating, sporulation, telomeric silencing and early stages of mitophagy; orthologous to human NAA15; expression of both human NAA10 and NAA15 functionally complements ard1 nat1 double mutant although single mutations are not complemented by their orthologs
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Nat1

Nat1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nat1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available