Standard name | |
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Human Ortholog | |
Description | Flavin adenine dinucleotide (FAD) synthetase; performs the second step in synthesis of FAD from riboflavin; mutation is functionally complemented by human FLAD1 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 0 | 0 | 2 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 2 | 3 | 7 |
Bud | 1 | 0 | 0 | 1 | 0 | 2 | 3 | – | 6 | 10 | 9 | 12 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 4 | 1 | 6 | 17 | 18 |
Bud Neck | 0 | 2 | 2 | 2 | 3 | 2 | 3 | – | 4 | 1 | 1 | 5 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 1 | 0 | 1 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 1 | 4 | 3 | 4 | 2 | 4 | – | 2 | 3 | 3 | 9 | 2 | 0 | 2 | 3 | 5 | 2 | 0 | 1 | 1 | 0 | 2 | 5 |
Cytoplasm | 456 | 419 | 374 | 303 | 238 | 252 | 377 | – | 281 | 313 | 271 | 524 | 712 | 864 | 917 | 577 | 339 | 358 | 438 | 387 | 342 | 481 | 562 | 593 |
Endoplasmic Reticulum | 1 | 4 | 0 | 0 | 3 | 0 | 2 | – | 0 | 1 | 2 | 2 | 1 | 0 | 0 | 32 | 11 | 14 | 1 | 1 | 1 | 1 | 2 | 3 |
Endosome | 1 | 1 | 1 | 0 | 0 | 1 | 0 | – | 0 | 4 | 1 | 2 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 3 | 1 | 1 | 3 | 3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 |
Mitochondria | 4 | 7 | 0 | 2 | 8 | 31 | 27 | – | 138 | 118 | 125 | 193 | 0 | 0 | 0 | 16 | 4 | 1 | 4 | 4 | 5 | 1 | 2 | 6 |
Nucleus | 0 | 0 | 0 | 0 | 2 | 2 | 6 | – | 4 | 1 | 3 | 12 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 2 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 2 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 0 | 0 | 0 | 1 | 1 | – | 1 | 1 | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 5 | 7 | 13 |
Vac/Vac Membrane | 3 | 1 | 0 | 0 | 2 | 9 | 9 | – | 4 | 17 | 13 | 18 | 1 | 0 | 2 | 4 | 9 | 2 | 1 | 4 | 0 | 3 | 3 | 3 |
Unique Cell Count | 463 | 431 | 375 | 304 | 249 | 280 | 406 | 370 | 399 | 367 | 671 | 716 | 865 | 919 | 585 | 345 | 361 | 459 | 422 | 370 | 518 | 619 | 683 | |
Labelled Cell Count | 469 | 438 | 381 | 312 | 261 | 303 | 434 | 443 | 473 | 429 | 783 | 718 | 865 | 922 | 635 | 369 | 380 | 459 | 422 | 370 | 518 | 619 | 683 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.5 | 4.2 | 5.1 | 4.7 | 4.7 | 4.7 | 4.9 | – | 3.7 | 4.4 | 4.2 | 4.3 | 5.3 | 5.4 | 4.9 | 5.1 | 6.4 | 6.2 | 5.7 | 5.9 | 5.8 |
Std Deviation (1e-4) | 0.5 | 0.8 | 0.9 | 1.1 | 1.2 | 1.3 | 1.3 | – | 0.9 | 1.2 | 1.0 | 1.0 | 1.1 | 1.1 | 1.1 | 0.9 | 1.2 | 1.0 | 1.7 | 1.6 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.13 | -0.12 | -0.07 | – | -0.44 | -0.2 | -0.3 | -0.24 | 0.06 | 0.07 | -0.06 | 0.0 | 0.32 | 0.28 | 0.17 | 0.2 | 0.18 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.9471 | 4.0881 | 4.155 | 4.2246 | 3.887 | 4.5267 | 4.9312 | 5.4578 | 4.8171 | 4.4153 | 5.7923 | 5.1126 | 2.5967 | 2.2032 | 2.5505 | 2.682 | 2.4371 | 2.2951 |
Actin | 0.0194 | 0.0008 | 0.0163 | 0.0333 | 0.0044 | 0.0047 | 0.0145 | 0.0003 | 0.0017 | 0.0014 | 0.0004 | 0.0017 | 0.0362 | 0.0014 | 0.0086 | 0.0569 | 0.0533 | 0.008 |
Bud | 0.0006 | 0.001 | 0.0027 | 0.0013 | 0.003 | 0.0007 | 0.0008 | 0.0009 | 0.0027 | 0.0001 | 0.0001 | 0.0009 | 0.0033 | 0.0033 | 0.0036 | 0.0002 | 0.0078 | 0.002 |
Bud Neck | 0.003 | 0.0004 | 0.0041 | 0.0008 | 0.0004 | 0.0018 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0003 | 0.0034 | 0.0004 | 0.001 | 0.0051 | 0.0014 | 0.0005 |
Bud Periphery | 0.0014 | 0.0034 | 0.0048 | 0.0012 | 0.0037 | 0.0013 | 0.0012 | 0.0014 | 0.0032 | 0.0001 | 0.0001 | 0.0009 | 0.0131 | 0.0035 | 0.0014 | 0.0006 | 0.0146 | 0.0019 |
Bud Site | 0.0051 | 0.0119 | 0.0044 | 0.0008 | 0.0092 | 0.0008 | 0.0027 | 0.0044 | 0.0015 | 0.0002 | 0.0001 | 0.0001 | 0.0028 | 0.0078 | 0.0075 | 0.0006 | 0.0014 | 0.0002 |
Cell Periphery | 0.0004 | 0.0006 | 0.0005 | 0.0001 | 0.0006 | 0.0001 | 0.0003 | 0.0006 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0003 | 0.0002 | 0.0002 | 0.0003 | 0.0001 |
Cytoplasm | 0.1196 | 0.1913 | 0.1562 | 0.1615 | 0.1366 | 0.2411 | 0.1787 | 0.2319 | 0.2237 | 0.2349 | 0.1422 | 0.304 | 0.0737 | 0.2056 | 0.0756 | 0.1403 | 0.1513 | 0.1621 |
Cytoplasmic Foci | 0.0227 | 0.0038 | 0.0117 | 0.0054 | 0.0155 | 0.0088 | 0.0079 | 0.0024 | 0.0057 | 0.0064 | 0.0021 | 0.0037 | 0.0374 | 0.0036 | 0.0133 | 0.0029 | 0.0091 | 0.0102 |
Eisosomes | 0.0003 | 0.0002 | 0.0004 | 0.0003 | 0.0002 | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0005 | 0.0002 | 0.0002 | 0.0005 | 0.0004 | 0.0001 |
Endoplasmic Reticulum | 0.0016 | 0.0043 | 0.0026 | 0.0015 | 0.0017 | 0.0016 | 0.0085 | 0.0011 | 0.0039 | 0.0039 | 0.0006 | 0.0019 | 0.0023 | 0.0013 | 0.0022 | 0.0006 | 0.0047 | 0.0009 |
Endosome | 0.0139 | 0.0012 | 0.0037 | 0.0015 | 0.0256 | 0.0019 | 0.013 | 0.0002 | 0.0017 | 0.0033 | 0.0003 | 0.0017 | 0.0066 | 0.0005 | 0.0033 | 0.0005 | 0.0133 | 0.0071 |
Golgi | 0.0048 | 0.0002 | 0.0029 | 0.0015 | 0.0053 | 0.0004 | 0.0024 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0033 | 0.0001 | 0.0008 | 0.0005 | 0.0049 | 0.0013 |
Lipid Particles | 0.0037 | 0.0002 | 0.0037 | 0.0018 | 0.0076 | 0.0022 | 0.0011 | 0.0001 | 0.0004 | 0.0006 | 0.0001 | 0.0001 | 0.0137 | 0.0001 | 0.0019 | 0.0002 | 0.005 | 0.0014 |
Mitochondria | 0.0013 | 0.001 | 0.0029 | 0.0007 | 0.0201 | 0.0007 | 0.002 | 0.0002 | 0.0007 | 0.0003 | 0.0001 | 0.0002 | 0.0026 | 0.0003 | 0.0004 | 0.0002 | 0.0046 | 0.0005 |
None | 0.773 | 0.7738 | 0.7602 | 0.7809 | 0.7519 | 0.7182 | 0.7423 | 0.7527 | 0.7381 | 0.743 | 0.8518 | 0.6761 | 0.7379 | 0.7671 | 0.8513 | 0.789 | 0.657 | 0.794 |
Nuclear Periphery | 0.0022 | 0.0013 | 0.0013 | 0.0009 | 0.0014 | 0.0021 | 0.0119 | 0.0004 | 0.0057 | 0.0013 | 0.0004 | 0.0029 | 0.009 | 0.0005 | 0.0031 | 0.0002 | 0.0248 | 0.0015 |
Nucleolus | 0.0007 | 0.0002 | 0.0007 | 0.0002 | 0.0002 | 0.0015 | 0.0005 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0014 | 0.0002 | 0.0005 | 0.0001 | 0.001 | 0.0002 |
Nucleus | 0.0014 | 0.002 | 0.0018 | 0.0014 | 0.0016 | 0.0043 | 0.0041 | 0.0013 | 0.0062 | 0.0012 | 0.0004 | 0.0018 | 0.0101 | 0.0017 | 0.0023 | 0.0005 | 0.0046 | 0.0009 |
Peroxisomes | 0.0156 | 0.0001 | 0.0125 | 0.0021 | 0.0056 | 0.0043 | 0.0007 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0235 | 0.0002 | 0.0061 | 0.0003 | 0.0048 | 0.0011 |
Punctate Nuclear | 0.0076 | 0.0011 | 0.0057 | 0.0019 | 0.0035 | 0.0029 | 0.0034 | 0.0007 | 0.0018 | 0.0011 | 0.0005 | 0.0028 | 0.0167 | 0.0012 | 0.0161 | 0.0003 | 0.0336 | 0.0056 |
Vacuole | 0.0015 | 0.001 | 0.0006 | 0.0005 | 0.001 | 0.0004 | 0.002 | 0.0007 | 0.0011 | 0.001 | 0.0002 | 0.0005 | 0.0015 | 0.0006 | 0.0005 | 0.0002 | 0.001 | 0.0003 |
Vacuole Periphery | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0008 | 0.0001 | 0.0011 | 0.0001 | 0.0004 | 0.0001 | 0 | 0.0001 | 0.0007 | 0.0001 | 0.0002 | 0 | 0.0013 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.7825 | 4.2839 | 11.126 | 15.7146 | 8.5433 | 14.3686 | 13.2652 | 13.8864 | 13.9249 | 18.8535 |
Translational Efficiency | 1.298 | 1.7151 | 0.8817 | 0.5949 | 1.4479 | 0.9991 | 0.89 | 0.7842 | 0.9323 | 0.6575 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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