Standard name
Human Ortholog
Description Flavin adenine dinucleotide (FAD) synthetase; performs the second step in synthesis of FAD from riboflavin; mutation is functionally complemented by human FLAD1

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.97 1.0 1.0 0.96 0.9 0.93 0.76 0.78 0.74 0.78 0.99 1.0 1.0 0.99 0.98 0.99 0.96 0.92 0.92 0.93 0.91 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.11 0.07 0.37 0.3 0.34 0.29 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 2 0 0 0 1 0 0 0 0 0 0 0 2 3 2 3 7
Bud 1 0 0 1 0 2 3 6 10 9 12 0 0 1 0 0 0 1 4 1 6 17 18
Bud Neck 0 2 2 2 3 2 3 4 1 1 5 0 0 0 1 1 0 2 0 1 0 1 4
Bud Site 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cell Periphery 3 1 4 3 4 2 4 2 3 3 9 2 0 2 3 5 2 0 1 1 0 2 5
Cytoplasm 456 419 374 303 238 252 377 281 313 271 524 712 864 917 577 339 358 438 387 342 481 562 593
Endoplasmic Reticulum 1 4 0 0 3 0 2 0 1 2 2 1 0 0 32 11 14 1 1 1 1 2 3
Endosome 1 1 1 0 0 1 0 0 4 1 2 0 1 0 1 0 1 1 3 1 1 3 3
Golgi 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 0 0
Mitochondria 4 7 0 2 8 31 27 138 118 125 193 0 0 0 16 4 1 4 4 5 1 2 6
Nucleus 0 0 0 0 2 2 6 4 1 3 12 0 0 0 1 0 2 0 0 0 1 0 2
Nuclear Periphery 0 1 0 0 0 0 0 2 1 0 2 0 0 0 0 0 0 0 0 0 0 1 2
Nucleolus 0 0 0 0 1 0 0 0 3 0 0 1 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
SpindlePole 0 1 0 0 0 1 1 1 1 1 3 1 0 0 0 0 0 0 2 2 5 7 13
Vac/Vac Membrane 3 1 0 0 2 9 9 4 17 13 18 1 0 2 4 9 2 1 4 0 3 3 3
Unique Cell Count 463 431 375 304 249 280 406 370 399 367 671 716 865 919 585 345 361 459 422 370 518 619 683
Labelled Cell Count 469 438 381 312 261 303 434 443 473 429 783 718 865 922 635 369 380 459 422 370 518 619 683


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 4.2 5.1 4.7 4.7 4.7 4.9 3.7 4.4 4.2 4.3 5.3 5.4 4.9 5.1 6.4 6.2 5.7 5.9 5.8
Std Deviation (1e-4) 0.5 0.8 0.9 1.1 1.2 1.3 1.3 0.9 1.2 1.0 1.0 1.1 1.1 1.1 0.9 1.2 1.0 1.7 1.6 1.5
Intensity Change (Log2) -0.11 -0.13 -0.12 -0.07 -0.44 -0.2 -0.3 -0.24 0.06 0.07 -0.06 0.0 0.32 0.28 0.17 0.2 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.9471 4.0881 4.155 4.2246 3.887 4.5267 4.9312 5.4578 4.8171 4.4153 5.7923 5.1126 2.5967 2.2032 2.5505 2.682 2.4371 2.2951
Actin 0.0194 0.0008 0.0163 0.0333 0.0044 0.0047 0.0145 0.0003 0.0017 0.0014 0.0004 0.0017 0.0362 0.0014 0.0086 0.0569 0.0533 0.008
Bud 0.0006 0.001 0.0027 0.0013 0.003 0.0007 0.0008 0.0009 0.0027 0.0001 0.0001 0.0009 0.0033 0.0033 0.0036 0.0002 0.0078 0.002
Bud Neck 0.003 0.0004 0.0041 0.0008 0.0004 0.0018 0.0004 0.0001 0.0002 0.0001 0 0.0003 0.0034 0.0004 0.001 0.0051 0.0014 0.0005
Bud Periphery 0.0014 0.0034 0.0048 0.0012 0.0037 0.0013 0.0012 0.0014 0.0032 0.0001 0.0001 0.0009 0.0131 0.0035 0.0014 0.0006 0.0146 0.0019
Bud Site 0.0051 0.0119 0.0044 0.0008 0.0092 0.0008 0.0027 0.0044 0.0015 0.0002 0.0001 0.0001 0.0028 0.0078 0.0075 0.0006 0.0014 0.0002
Cell Periphery 0.0004 0.0006 0.0005 0.0001 0.0006 0.0001 0.0003 0.0006 0.0003 0.0001 0.0001 0.0001 0.0004 0.0003 0.0002 0.0002 0.0003 0.0001
Cytoplasm 0.1196 0.1913 0.1562 0.1615 0.1366 0.2411 0.1787 0.2319 0.2237 0.2349 0.1422 0.304 0.0737 0.2056 0.0756 0.1403 0.1513 0.1621
Cytoplasmic Foci 0.0227 0.0038 0.0117 0.0054 0.0155 0.0088 0.0079 0.0024 0.0057 0.0064 0.0021 0.0037 0.0374 0.0036 0.0133 0.0029 0.0091 0.0102
Eisosomes 0.0003 0.0002 0.0004 0.0003 0.0002 0.0001 0.0004 0.0002 0.0001 0.0002 0.0001 0.0001 0.0005 0.0002 0.0002 0.0005 0.0004 0.0001
Endoplasmic Reticulum 0.0016 0.0043 0.0026 0.0015 0.0017 0.0016 0.0085 0.0011 0.0039 0.0039 0.0006 0.0019 0.0023 0.0013 0.0022 0.0006 0.0047 0.0009
Endosome 0.0139 0.0012 0.0037 0.0015 0.0256 0.0019 0.013 0.0002 0.0017 0.0033 0.0003 0.0017 0.0066 0.0005 0.0033 0.0005 0.0133 0.0071
Golgi 0.0048 0.0002 0.0029 0.0015 0.0053 0.0004 0.0024 0.0001 0.0002 0.0002 0 0.0001 0.0033 0.0001 0.0008 0.0005 0.0049 0.0013
Lipid Particles 0.0037 0.0002 0.0037 0.0018 0.0076 0.0022 0.0011 0.0001 0.0004 0.0006 0.0001 0.0001 0.0137 0.0001 0.0019 0.0002 0.005 0.0014
Mitochondria 0.0013 0.001 0.0029 0.0007 0.0201 0.0007 0.002 0.0002 0.0007 0.0003 0.0001 0.0002 0.0026 0.0003 0.0004 0.0002 0.0046 0.0005
None 0.773 0.7738 0.7602 0.7809 0.7519 0.7182 0.7423 0.7527 0.7381 0.743 0.8518 0.6761 0.7379 0.7671 0.8513 0.789 0.657 0.794
Nuclear Periphery 0.0022 0.0013 0.0013 0.0009 0.0014 0.0021 0.0119 0.0004 0.0057 0.0013 0.0004 0.0029 0.009 0.0005 0.0031 0.0002 0.0248 0.0015
Nucleolus 0.0007 0.0002 0.0007 0.0002 0.0002 0.0015 0.0005 0.0002 0.0003 0.0001 0.0001 0.0002 0.0014 0.0002 0.0005 0.0001 0.001 0.0002
Nucleus 0.0014 0.002 0.0018 0.0014 0.0016 0.0043 0.0041 0.0013 0.0062 0.0012 0.0004 0.0018 0.0101 0.0017 0.0023 0.0005 0.0046 0.0009
Peroxisomes 0.0156 0.0001 0.0125 0.0021 0.0056 0.0043 0.0007 0.0001 0.0003 0.0003 0.0001 0.0001 0.0235 0.0002 0.0061 0.0003 0.0048 0.0011
Punctate Nuclear 0.0076 0.0011 0.0057 0.0019 0.0035 0.0029 0.0034 0.0007 0.0018 0.0011 0.0005 0.0028 0.0167 0.0012 0.0161 0.0003 0.0336 0.0056
Vacuole 0.0015 0.001 0.0006 0.0005 0.001 0.0004 0.002 0.0007 0.0011 0.001 0.0002 0.0005 0.0015 0.0006 0.0005 0.0002 0.001 0.0003
Vacuole Periphery 0.0004 0.0002 0.0002 0.0001 0.0008 0.0001 0.0011 0.0001 0.0004 0.0001 0 0.0001 0.0007 0.0001 0.0002 0 0.0013 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.7825 4.2839 11.126 15.7146 8.5433 14.3686 13.2652 13.8864 13.9249 18.8535
Translational Efficiency 1.298 1.7151 0.8817 0.5949 1.4479 0.9991 0.89 0.7842 0.9323 0.6575

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Flavin adenine dinucleotide (FAD) synthetase; performs the second step in synthesis of FAD from riboflavin; mutation is functionally complemented by human FLAD1
Localization
Cell Percentages cytoplasm (48%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Fad1

Fad1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fad1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available