Standard name
Human Ortholog
Description Protein with similarity to mammalian monocarboxylate permeases; monocarboxylate permeases are involved in transport of monocarboxylic acids across the plasma membrane but mutant is not deficient in monocarboxylate transport
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.08 0.18 – 0.24 0.34 0.38 0.49 0.51 0.06 0.06 0 0.12 0.14 0.15 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.07 0 0 0 0.06 – 0.05 0.08 0.09 0.16 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.15 0.08 0.06 0 0 0.05 – 0.05 0 0 0 0 0.12 0.12 0.14 0.16 0.14 0.17 0.06 0.06 0 0.07 0.05 0
Nuclear Periphery 0.05 0 0.13 0.16 0.06 0.08 – 0.06 0.06 0 0 0 0 0.05 0 0.08 0.05 0.06 0 0 0.07 0 0 0
Nucleolus 0.11 0.06 0.06 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.75 0.82 0.86 0.89 0.85 0.75 – 0.71 0.59 0.58 0.48 0.45 0.84 0.83 0.84 0.72 0.73 0.7 0.83 0.84 0.85 0.82 0.81 0.83
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 2 1 2 4 4 0 0 0 0 0 0 2 0 0 1 6 13
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 0 3 – 13 7 7 10 3 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 5 13 15 27 71 – 148 149 203 193 215 20 20 11 8 12 13 0 0 0 0 3 2
Endoplasmic Reticulum 0 1 0 1 3 2 – 14 20 8 8 12 0 0 0 2 1 4 0 0 1 0 1 2
Endosome 1 1 6 5 7 5 – 10 3 6 4 5 7 10 7 0 1 0 4 6 9 9 33 33
Golgi 2 0 2 5 4 2 – 1 0 0 0 0 3 3 2 0 0 3 0 0 0 1 2 3
Mitochondria 7 12 8 11 10 22 – 30 37 49 61 56 0 0 3 0 2 0 3 1 5 2 7 9
Nucleus 26 13 21 4 14 20 – 29 16 8 6 4 41 41 44 10 12 15 12 11 6 20 31 20
Nuclear Periphery 8 7 43 46 21 31 – 35 25 9 5 6 12 16 8 5 4 5 3 2 23 7 30 28
Nucleolus 19 10 19 8 9 4 – 2 4 2 1 2 9 9 9 1 3 2 6 5 2 5 7 5
Peroxisomes 0 0 0 0 0 1 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 1 1 0 0 0 2 2
Vac/Vac Membrane 128 142 292 263 285 294 – 442 263 310 187 193 289 293 271 46 61 62 176 162 305 243 560 607
Unique Cell Count 171 173 341 296 335 394 621 444 538 393 425 346 353 321 64 83 88 214 194 358 295 693 731
Labelled Cell Count 196 191 404 359 380 455 729 526 604 479 500 381 392 355 72 96 105 214 194 358 295 693 731


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.6 15.2 14.2 13.5 12.2 9.7 – 8.8 7.8 7.1 7.2 6.9 11.8 11.8 11.2 15.4 13.6 18.0 13.2 14.1 14.9
Std Deviation (1e-4) 5.0 4.8 4.0 3.4 3.2 3.0 – 2.4 2.2 1.9 1.9 1.6 2.7 2.7 2.6 5.2 5.2 6.8 3.7 3.9 4.0
Intensity Change (Log2) – – – -0.07 -0.22 -0.55 – -0.69 -0.86 -1.0 -0.98 -1.05 -0.26 -0.26 -0.34 0.12 -0.06 0.34 -0.1 -0.01 0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.8 2.3 6.0 – 8.0 10.2 11.4 13.6 14.1 1.2 1.1 -0.3 0 0 3.8
Endoplasmic Reticulum 0 0 0 – 0 4.0 0 0 3.1 0 0 0 0 0 0
Endosome -0.1 0.3 -0.5 – -0.2 0 0 0 0 0.3 0.9 0.4 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.0 0.5 2.2 – 1.9 3.6 4.0 6.1 5.4 0 0 -1.4 0 0 0
Nucleus -3.1 -1.2 -0.6 – -1.0 -1.7 -3.8 -3.3 -4.0 2.6 2.5 3.3 0 2.5 3.3
Nuclear Periphery 1.1 -2.8 -2.1 – -3.8 -3.4 -6.7 -6.2 -6.3 -4.4 -3.8 -4.9 -1.1 -2.0 -1.8
Nucleolus -1.8 -1.9 -3.5 – -5.3 -3.8 -4.9 -4.4 -4.3 -2.0 -2.0 -1.8 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.2 -0.2 -3.7 – -5.0 -8.1 -8.7 -10.8 -11.5 -0.8 -1.0 -0.4 -2.7 -2.7 -3.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.4662 12.7462 10.8677 10.2665 10.18 10.6951 10.9002 13.926 12.8932 12.539 12.4264 12.7637 12.8905 15.2567 13.6876 13.3758 13.3197 14.1207
Actin 0.028 0 0.0016 0.0017 0.0874 0.0007 0.0078 0 0.0083 0.0002 0 0 0.0142 0.0004 0.0133 0 0.0131 0.0001
Bud 0.0006 0.0001 0.0002 0.0001 0.0014 0.0001 0.0003 0.0001 0.0002 0.0002 0.0001 0.0001 0.0002 0.0001 0.0005 0 0.0003 0.0001
Bud Neck 0.0012 0.0001 0.0001 0.0001 0.0011 0.0001 0.0004 0.0001 0.0011 0.0001 0 0.0001 0.0004 0.0001 0.0007 0 0.0004 0.0001
Bud Periphery 0.0015 0.0001 0.0004 0.0002 0.0029 0.0003 0.0006 0.0003 0.0003 0.0008 0.0003 0.0003 0.0007 0.0003 0.0007 0.0001 0.0007 0.0003
Bud Site 0.0016 0.0001 0.0003 0.0002 0.0066 0.0002 0.0015 0.0001 0.0033 0.0001 0.0001 0.0001 0.0005 0.0007 0.004 0 0.0017 0
Cell Periphery 0.0016 0.0009 0.0011 0.0007 0.0006 0.0008 0.0021 0.0016 0.001 0.0005 0.0004 0.0005 0.0019 0.0013 0.0016 0.0011 0.0014 0.0008
Cytoplasm 0.0024 0.0057 0.0004 0.0014 0.0007 0.0001 0.0014 0.0007 0.0024 0.0002 0.0001 0.0001 0.0007 0.0002 0.0045 0.0001 0.0018 0.0001
Cytoplasmic Foci 0.0087 0.0002 0.0006 0.0367 0.0035 0.0003 0.0085 0.0001 0.0048 0.0006 0.0002 0.0005 0.0013 0.0001 0.0015 0 0.0063 0.0005
Eisosomes 0.0005 0 0 0 0.0006 0 0.0001 0 0 0 0 0 0.0004 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0065 0.0001 0.0006 0.0017 0.0011 0.0005 0.0016 0.0003 0.0007 0.0019 0.0003 0.0002 0.0016 0.0004 0.0005 0.0001 0.0046 0.0003
Endosome 0.021 0.0043 0.0144 0.0268 0.0123 0.0063 0.0288 0.0051 0.0129 0.0218 0.0106 0.0113 0.0107 0.008 0.013 0.0046 0.0207 0.0084
Golgi 0.005 0.0001 0.0019 0.0022 0.006 0.0004 0.0045 0.0001 0.0016 0.0031 0.0002 0.0005 0.0014 0.0005 0.0028 0.0001 0.0087 0.0023
Lipid Particles 0.0126 0.0004 0.0019 0.0175 0.0087 0.002 0.0072 0.0007 0.0031 0.0027 0.0011 0.0026 0.0013 0.0006 0.0093 0.0003 0.0149 0.0011
Mitochondria 0.0103 0.0012 0.0087 0.0026 0.0078 0.0108 0.0164 0.0041 0.0119 0.0316 0.0022 0.0063 0.0101 0.0041 0.0061 0.0005 0.005 0.0029
None 0.0078 0 0.0001 0.0013 0.0029 0.0001 0.0004 0 0.0007 0.0001 0 0 0.0003 0.0001 0.0001 0 0.0021 0
Nuclear Periphery 0.0283 0.006 0.0013 0.0021 0.0107 0.0135 0.0076 0.0068 0.0125 0.005 0.0025 0.0112 0.0167 0.0079 0.005 0.0009 0.0176 0.0157
Nucleolus 0.0073 0.0084 0.0009 0.0005 0.0086 0.0033 0.0075 0.0025 0.0031 0.0003 0.0006 0.001 0.0041 0.0013 0.0025 0.0007 0.0038 0.0012
Nucleus 0.0119 0.0092 0.0012 0.001 0.0067 0.0021 0.0168 0.002 0.0124 0.0008 0.0008 0.0014 0.0054 0.002 0.0041 0.0007 0.0051 0.0011
Peroxisomes 0.0157 0 0.0002 0.0062 0.0044 0.0011 0.0014 0.0001 0.002 0.0004 0 0.0001 0.0007 0 0.0007 0 0.0017 0.0001
Punctate Nuclear 0.0029 0.0003 0 0.018 0.0129 0.0001 0.0007 0 0.0006 0 0 0 0.0041 0 0.0001 0 0.0021 0
Vacuole 0.3069 0.3897 0.3969 0.4215 0.3348 0.3129 0.3197 0.3388 0.3054 0.3055 0.2454 0.2307 0.334 0.3355 0.3555 0.4129 0.2671 0.2639
Vacuole Periphery 0.5178 0.5731 0.5671 0.4573 0.4782 0.6443 0.5649 0.6366 0.6117 0.624 0.7348 0.7332 0.5891 0.6364 0.5736 0.5778 0.6205 0.701

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.3705 11.4773 9.5186 13.0614 12.7296 12.832 17.7484 14.2826 12.9869 19.5943
Translational Efficiency 0.9536 1.1853 1.1349 0.7867 0.9479 1.552 0.9408 0.917 1.0637 0.8125

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein with similarity to mammalian monocarboxylate permeases; monocarboxylate permeases are involved in transport of monocarboxylic acids across the plasma membrane but mutant is not deficient in monocarboxylate transport
Localization
Cell Percentages vacuolar membrane (89%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-2

Mch1

Mch1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mch1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available