Standard name
Human Ortholog
Description Transport adaptor or symportin; facilitates synchronized nuclear coimport of the two 5S-rRNA binding proteins Rpl5p and Rpl11p; binds to nascent Rpl5p during translation; required for biogenesis of the large ribosomal subunit; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.06 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.16 0.07 0.16 0.09 0.09 0.11 0.1 0.12 0.1 0.08 0.09 0.07 – 0.39 0.29 0.07 0.11 0.09 0.08 0 0.07 0.18 0.06 0.07
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.06 0 0.16 0.06 0.34 0.12 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.39 0.47 0.49 0.86 0.88 0.79 0.79 0.76 0.79 0.82 0.72 0.74 – 0.38 0.55 0.68 0.7 0.7 0.7 0.76 0.63 0.55 0.54 0.45
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0.06 0 0.07 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.59 0.58 0.56 0.07 0 0.05 0.08 0.06 0 0.06 0 0.12 – 0.29 0.16 0.26 0.21 0.2 0.12 0.12 0.21 0.13 0.15 0.25
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 1 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 1 0
Bud 1 0 0 0 0 0 1 1 2 1 0 0 – 0 0 0 0 0 12 4 7 0 4 1
Bud Neck 0 0 0 0 0 0 1 0 0 2 0 0 – 0 0 0 0 0 0 1 0 0 0 1
Bud Site 0 0 0 0 0 1 2 0 3 2 4 8 – 0 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 62 25 33 19 22 30 41 37 28 21 22 15 – 40 36 17 23 18 34 11 14 8 4 10
Endoplasmic Reticulum 0 3 0 0 0 0 0 1 0 0 0 0 – 1 0 5 6 2 0 1 1 0 1 4
Endosome 2 4 1 0 0 2 2 1 0 2 0 3 – 2 1 2 0 3 5 5 1 1 3 6
Golgi 0 1 0 0 0 1 0 0 0 0 0 0 – 0 1 2 6 0 1 2 0 0 0 1
Mitochondria 5 0 0 1 1 15 25 11 44 16 88 26 – 0 1 3 2 0 2 5 2 0 0 2
Nucleus 152 161 99 191 226 221 308 240 218 229 186 167 – 39 68 157 152 135 288 293 130 24 39 61
Nuclear Periphery 0 0 0 3 0 2 3 1 2 0 1 1 – 0 0 1 1 0 2 1 0 0 1 1
Nucleolus 1 0 0 2 2 4 6 9 17 5 18 8 – 2 1 5 2 7 4 5 0 0 1 4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 2 3 5 1 4 3 5 – 0 2 0 0 0 5 5 0 0 1 3
Vac/Vac Membrane 230 196 113 16 8 13 30 20 11 17 10 26 – 30 20 60 46 39 48 44 43 5 11 34
Unique Cell Count 387 340 202 221 257 279 391 314 275 278 258 225 103 123 231 216 194 410 386 206 45 74 137
Labelled Cell Count 453 390 246 234 259 291 423 326 326 299 332 260 114 131 252 238 204 410 386 206 45 74 137


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.1 11.9 10.0 9.9 9.7 7.4 7.3 7.4 6.3 6.7 5.9 6.2 – 9.3 9.2 16.4 18.0 16.6 – 10.2 9.8
Std Deviation (1e-4) 1.9 2.3 1.5 2.1 2.2 1.7 1.9 1.6 1.5 1.5 1.6 1.3 – 2.1 2.6 4.5 4.7 4.6 – 2.0 2.2
Intensity Change (Log2) – – – -0.01 -0.04 -0.44 -0.45 -0.42 -0.65 -0.58 -0.77 -0.68 – -0.1 -0.12 0.72 0.85 0.74 – 0.03 -0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Cytoplasm -2.4 -2.5 -1.8 -2.0 -1.5 -2.0 -3.0 -2.6 -3.2 – 4.4 2.8 -2.9 -1.7 -2.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Mitochondria 0 0 3.3 3.7 0 6.0 3.5 9.2 5.0 – 0 0 0 0 0
Nucleus 8.3 9.1 6.9 7.4 6.4 6.9 7.8 5.1 5.4 – -1.8 1.1 4.0 4.5 4.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nucleolus 0 0 0 0 0 3.6 0 3.8 0 – 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Vacuole -10.9 -12.8 -12.6 -13.0 -12.6 -12.8 -12.1 -12.5 -9.8 – -4.4 -7.1 -6.4 -7.3 -7.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.6011 8.3277 7.0024 7.5008 7.3912 7.1576 5.5471 7.8489 7.1574 6.6817 6.2097 6.7907 6.1508 8.429 7.3312 6.6019 6.2706 7.3894
Actin 0.0195 0.0008 0.0054 0.0005 0 0.0001 0.0271 0 0.006 0.0304 0.0031 0.0172 0.014 0 0.0019 0.0165 0.0202 0.002
Bud 0.0003 0.0009 0.0042 0.0001 0 0.0003 0.0006 0 0.0009 0.0006 0.0017 0.0004 0.0002 0 0.0002 0.0015 0.0013 0.0001
Bud Neck 0.0011 0.0004 0.0038 0.0003 0.0012 0.0021 0.0031 0.0001 0.0025 0.0048 0.001 0.0026 0.0012 0.0001 0.0002 0.0008 0.0025 0.0012
Bud Periphery 0.0009 0.0003 0.0044 0.0001 0.0001 0.0013 0.001 0 0.0018 0.0006 0.0039 0.0011 0.0002 0 0.0003 0.0011 0.003 0.0002
Bud Site 0.0009 0.002 0.0135 0.0001 0.0001 0.0003 0.0037 0 0.0061 0.0026 0.0019 0.0008 0.0086 0.0001 0.0017 0.0011 0.001 0.0001
Cell Periphery 0.0005 0.0001 0.0006 0 0 0.0005 0.0005 0 0.0007 0.0001 0.0003 0.0005 0.0001 0 0.0001 0.0002 0.0005 0
Cytoplasm 0.0048 0.0031 0.0009 0.0011 0.0006 0.0008 0.007 0.0065 0.0013 0.0053 0.0141 0.0012 0.0041 0.0017 0.0024 0.0632 0.0054 0.0006
Cytoplasmic Foci 0.0123 0.0002 0.0067 0.0003 0 0 0.011 0 0.0019 0.0094 0.0108 0.0058 0.0241 0 0.006 0.0073 0.0074 0
Eisosomes 0.0002 0 0.0001 0 0 0 0.0005 0 0.0002 0.0001 0 0.0001 0.0001 0 0 0.0001 0.0001 0
Endoplasmic Reticulum 0.004 0.0002 0.0009 0.0006 0.0001 0.0004 0.0036 0.0001 0.0008 0.0036 0.0055 0.0015 0.0009 0 0.0004 0.0008 0.0028 0.0004
Endosome 0.0126 0.0029 0.0072 0.0004 0.0001 0.0001 0.0171 0.0001 0.0051 0.0168 0.0332 0.0128 0.0094 0 0.0091 0.0179 0.0067 0.0005
Golgi 0.0014 0.0028 0.0021 0 0 0 0.007 0 0.0036 0.0069 0.0098 0.0052 0.0044 0 0.0077 0.0443 0.0067 0.0001
Lipid Particles 0.0077 0.0003 0.004 0 0 0 0.022 0 0.0027 0.0042 0.0267 0.006 0.0249 0 0.0044 0.0054 0.0081 0.0001
Mitochondria 0.0259 0.0007 0.0077 0.0007 0.0002 0.0005 0.0131 0.0001 0.006 0.0023 0.0116 0.0048 0.0019 0.0002 0.015 0.0153 0.0101 0.0043
None 0.0054 0.0001 0.0004 0.0001 0 0 0.0035 0 0.0003 0.0009 0.0021 0.0005 0.0014 0 0.001 0.001 0.0007 0.0001
Nuclear Periphery 0.0201 0.0042 0.0054 0.0267 0.0037 0.0012 0.0243 0.0028 0.0049 0.0054 0.0403 0.0041 0.0115 0.0024 0.0061 0.0046 0.021 0.0102
Nucleolus 0.0222 0.0058 0.0087 0.0084 0.0087 0.0236 0.0168 0.0044 0.0069 0.0119 0.0168 0.0193 0.0066 0.0068 0.0168 0.0053 0.0275 0.0287
Nucleus 0.8285 0.9707 0.9107 0.9294 0.9701 0.9642 0.8095 0.9845 0.942 0.8814 0.7292 0.9014 0.8482 0.9872 0.9085 0.7824 0.8383 0.9476
Peroxisomes 0.0096 0.0001 0.0069 0 0 0 0.0102 0 0.0014 0.0064 0.0066 0.0047 0.0265 0 0.006 0.0013 0.0239 0
Punctate Nuclear 0.0137 0.0034 0.0006 0.0301 0.0141 0.0016 0.0129 0.0012 0.0022 0.0053 0.0109 0.0057 0.011 0.0012 0.0052 0.0276 0.0062 0.003
Vacuole 0.0055 0.0008 0.0039 0.0004 0.0007 0.0024 0.0035 0.0002 0.0023 0.0006 0.0447 0.0024 0.0006 0.0002 0.0048 0.0012 0.0045 0.0004
Vacuole Periphery 0.0029 0.0004 0.0019 0.0005 0.0002 0.0004 0.002 0 0.0006 0.0004 0.0259 0.0019 0.0003 0.0002 0.002 0.0009 0.0022 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 54.7852 44.4743 37.7164 30.9419 36.4756 27.8214 34.7966 28.7244 17.6343 38.51
Translational Efficiency 0.9565 1.0852 1.0135 0.6931 0.7867 1.0771 0.9966 0.6538 0.8104 0.8182

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Transport adaptor or symportin; facilitates synchronized nuclear coimport of the two 5S-rRNA binding proteins Rpl5p and Rpl11p; binds to nascent Rpl5p during translation; required for biogenesis of the large ribosomal subunit; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
Localization
Cell Percentages nucleus (68%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-5

Syo1

Syo1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Syo1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available