Standard name | |
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Human Ortholog | |
Description | SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC) |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 7 | 2 | 3 | 5 | 7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 50 | 42 | 10 | 68 | 58 | 50 | 68 | – | 33 | 54 | 41 | 42 | 48 | 81 | 71 | 36 | 30 | 38 | 27 | 15 | 12 | 19 | 46 | 30 |
Endoplasmic Reticulum | 0 | 0 | 0 | 1 | 0 | 2 | 0 | – | 1 | 0 | 0 | 1 | 4 | 0 | 1 | 5 | 5 | 5 | 0 | 0 | 0 | 1 | 2 | 3 |
Endosome | 1 | 0 | 0 | 0 | 0 | 0 | 3 | – | 5 | 2 | 0 | 5 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 4 | 4 |
Golgi | 1 | 0 | 0 | 0 | 2 | 0 | 2 | – | 0 | 1 | 0 | 0 | 5 | 0 | 3 | 5 | 5 | 5 | 1 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1 | 0 | 0 | 0 | 0 | 14 | 0 | – | 3 | 8 | 14 | 14 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleus | 25 | 6 | 8 | 48 | 90 | 45 | 114 | – | 71 | 83 | 70 | 104 | 9 | 22 | 18 | 24 | 7 | 5 | 88 | 69 | 48 | 42 | 89 | 70 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 6 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 0 | 1 | – | 2 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 6 | 8 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 4 | 6 |
Vac/Vac Membrane | 90 | 61 | 29 | 69 | 167 | 71 | 141 | – | 78 | 92 | 66 | 87 | 78 | 114 | 111 | 86 | 51 | 59 | 4 | 8 | 6 | 14 | 27 | 29 |
Unique Cell Count | 131 | 86 | 42 | 148 | 273 | 159 | 296 | 178 | 216 | 174 | 239 | 118 | 187 | 180 | 127 | 82 | 98 | 140 | 103 | 74 | 89 | 204 | 175 | |
Labelled Cell Count | 169 | 109 | 47 | 187 | 317 | 182 | 330 | 194 | 242 | 193 | 258 | 146 | 219 | 204 | 157 | 101 | 114 | 140 | 103 | 74 | 89 | 204 | 175 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 14.0 | 15.8 | 13.2 | 12.3 | 12.9 | 8.2 | 11.0 | – | 9.5 | 10.0 | 9.0 | 9.9 | 15.6 | 15.9 | 16.3 | 23.8 | 24.0 | 23.2 | 13.8 | 14.2 | 15.3 |
Std Deviation (1e-4) | 2.9 | 2.5 | 3.4 | 2.0 | 1.9 | 2.1 | 1.9 | – | 1.9 | 1.8 | 1.7 | 2.9 | 2.7 | 3.0 | 2.7 | 6.2 | 6.7 | 6.6 | 2.8 | 2.6 | 2.6 |
Intensity Change (Log2) | – | – | – | -0.1 | -0.03 | -0.69 | -0.26 | – | -0.47 | -0.4 | -0.55 | -0.42 | 0.24 | 0.27 | 0.3 | 0.85 | 0.86 | 0.81 | 0.06 | 0.1 | 0.21 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.6 | -0.4 | 1.0 | -0.1 | – | -0.8 | 0.2 | 0 | -1.0 | 2.0 | 2.3 | 1.9 | 0.6 | 1.4 | 1.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 1.7 | 1.8 | 1.2 | 2.5 | – | 2.5 | 2.4 | 2.6 | 3.0 | 0 | -1.3 | 0 | 0 | -1.7 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -2.6 | -1.0 | -2.8 | -2.6 | – | -2.9 | -3.1 | -3.6 | -4.0 | -0.3 | -1.0 | -0.9 | -0.2 | -0.8 | -1.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.9549 | 8.3466 | 6.0276 | 6.4548 | 7.0101 | 6.2221 | 8.4503 | 11.6111 | 10.3052 | 7.838 | 8.9502 | 10.088 | 9.0349 | 11.8159 | 10.6988 | 10.9397 | 9.3581 | 10.6989 |
Actin | 0.0172 | 0.0002 | 0.0839 | 0.0011 | 0.0001 | 0.0042 | 0.0034 | 0.0063 | 0.0258 | 0.1604 | 0 | 0.0005 | 0.0423 | 0.0008 | 0.0152 | 0.0001 | 0.0759 | 0.0099 |
Bud | 0.0033 | 0.0001 | 0.0003 | 0.0005 | 0 | 0.0001 | 0.0002 | 0.0042 | 0.0003 | 0.0021 | 0 | 0.0004 | 0.0005 | 0.0012 | 0.0002 | 0.0001 | 0.0005 | 0.001 |
Bud Neck | 0.0057 | 0.0005 | 0.0028 | 0.0017 | 0.0005 | 0.0012 | 0.0002 | 0.0002 | 0.0002 | 0.0012 | 0.0001 | 0.0025 | 0.001 | 0.0003 | 0.0006 | 0.0003 | 0.0011 | 0.002 |
Bud Periphery | 0.0023 | 0 | 0.0003 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0025 | 0.0004 | 0.0036 | 0 | 0.0002 | 0.0007 | 0.0009 | 0.0002 | 0 | 0.0006 | 0.0008 |
Bud Site | 0.1168 | 0.0004 | 0.0031 | 0.0028 | 0 | 0.0003 | 0.0011 | 0.004 | 0.0004 | 0.0021 | 0 | 0.0001 | 0.0009 | 0.0069 | 0.0041 | 0 | 0.0026 | 0.0007 |
Cell Periphery | 0.001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0.0002 | 0.0002 | 0 | 0 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 |
Cytoplasm | 0.0787 | 0.1183 | 0.0447 | 0.0827 | 0.1066 | 0.0648 | 0.1207 | 0.1311 | 0.1954 | 0.0056 | 0.0025 | 0.1007 | 0.0943 | 0.0717 | 0.0975 | 0.0173 | 0.0173 | 0.0758 |
Cytoplasmic Foci | 0.0717 | 0.0003 | 0.0155 | 0.027 | 0.0001 | 0.0058 | 0.0418 | 0.0033 | 0.0037 | 0.0124 | 0 | 0.0003 | 0.0028 | 0.0055 | 0.0031 | 0 | 0.012 | 0.0032 |
Eisosomes | 0.0002 | 0 | 0.0013 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0004 | 0 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0044 | 0.002 | 0.01 | 0.0024 | 0.0001 | 0.002 | 0.0028 | 0.0005 | 0.0243 | 0.0026 | 0 | 0.002 | 0.0026 | 0.0006 | 0.0028 | 0.0001 | 0.0102 | 0.0042 |
Endosome | 0.0453 | 0.0012 | 0.0159 | 0.0564 | 0 | 0.0213 | 0.0196 | 0.0003 | 0.0082 | 0.0329 | 0 | 0.0012 | 0.0068 | 0.0079 | 0.0089 | 0 | 0.0392 | 0.0286 |
Golgi | 0.0283 | 0 | 0.0057 | 0.0071 | 0 | 0.005 | 0.0029 | 0.0004 | 0.0095 | 0.0446 | 0 | 0 | 0.0064 | 0.0038 | 0.0051 | 0 | 0.0036 | 0.0108 |
Lipid Particles | 0.014 | 0 | 0.0056 | 0.0461 | 0 | 0.0112 | 0.0148 | 0 | 0.0018 | 0.0382 | 0 | 0.0001 | 0.0048 | 0.0002 | 0.0017 | 0 | 0.0107 | 0.0041 |
Mitochondria | 0.012 | 0.0002 | 0.0012 | 0.0017 | 0.0001 | 0.0011 | 0.001 | 0.0279 | 0.0032 | 0.0414 | 0 | 0.0004 | 0.0534 | 0.0015 | 0.0175 | 0.0001 | 0.0014 | 0.004 |
None | 0.0119 | 0.0005 | 0.0037 | 0.0029 | 0.0001 | 0.0009 | 0.009 | 0.0003 | 0.0009 | 0.0007 | 0 | 0.0003 | 0.0009 | 0.0002 | 0.0013 | 0 | 0.0029 | 0.0008 |
Nuclear Periphery | 0.0384 | 0.0252 | 0.0451 | 0.0217 | 0.0081 | 0.0167 | 0.0185 | 0.0177 | 0.0304 | 0.0104 | 0.0047 | 0.0501 | 0.0252 | 0.0072 | 0.0106 | 0.0083 | 0.0273 | 0.0177 |
Nucleolus | 0.0017 | 0.0024 | 0.0006 | 0.0018 | 0.0004 | 0.0007 | 0.0008 | 0.0004 | 0.0003 | 0.0005 | 0.0003 | 0.0018 | 0.0016 | 0.0015 | 0.0004 | 0.0004 | 0.0011 | 0.0007 |
Nucleus | 0.4843 | 0.8238 | 0.7425 | 0.7175 | 0.8818 | 0.8559 | 0.7033 | 0.7961 | 0.6759 | 0.6289 | 0.9919 | 0.8325 | 0.7435 | 0.8605 | 0.8247 | 0.9717 | 0.7683 | 0.8245 |
Peroxisomes | 0.0111 | 0 | 0.0061 | 0.004 | 0 | 0.0016 | 0.0142 | 0.0018 | 0.001 | 0.0076 | 0 | 0 | 0.0017 | 0.0003 | 0.0033 | 0 | 0.0029 | 0.0013 |
Punctate Nuclear | 0.0442 | 0.0048 | 0.005 | 0.0113 | 0.0018 | 0.005 | 0.044 | 0.0012 | 0.0015 | 0.0018 | 0.0003 | 0.0048 | 0.0046 | 0.0007 | 0.0012 | 0.0011 | 0.0136 | 0.0063 |
Vacuole | 0.0061 | 0.0064 | 0.0045 | 0.0097 | 0.0001 | 0.0012 | 0.0011 | 0.0013 | 0.0102 | 0.0011 | 0 | 0.0012 | 0.0032 | 0.0214 | 0.0007 | 0.0002 | 0.0067 | 0.0022 |
Vacuole Periphery | 0.0012 | 0.0135 | 0.002 | 0.0013 | 0 | 0.0006 | 0.0005 | 0.0003 | 0.0062 | 0.0014 | 0 | 0.0007 | 0.0024 | 0.0066 | 0.001 | 0.0001 | 0.0017 | 0.0011 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 62.8522 | 68.6718 | 63.3107 | 62.5321 | 78.4724 | 79.1033 | 70.486 | 70.2948 | 50.7777 | 79.3305 |
Translational Efficiency | 2.0893 | 2.2629 | 1.6086 | 1.4725 | 1.768 | 1.8558 | 1.7383 | 1.5672 | 2.017 | 1.679 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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