Standard name | |
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Human Ortholog | |
Description | Subunit VIIa of cytochrome c oxidase (Complex IV); Complex IV is the terminal member of the mitochondrial inner membrane electron transport chain |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 3 | 4 |
Bud | 1 | 1 | 4 | 2 | 3 | 1 | – | 9 | 14 | 6 | 7 | 17 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 9 | 10 |
Bud Neck | 0 | 0 | 2 | 0 | 0 | 0 | – | 8 | 1 | 2 | 0 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 2 | 2 | 4 | – | 0 | 4 | 1 | 1 | 2 | 0 | 0 | 1 | 0 | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 335 | 53 | 186 | 387 | 480 | 334 | – | 559 | 360 | 356 | 203 | 243 | 248 | 353 | 469 | 61 | 58 | 73 | 324 | 71 | 170 | 120 | 302 | 287 |
Endoplasmic Reticulum | 0 | 0 | 1 | 0 | 0 | 2 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 3 | 7 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 0 | 1 | 0 | 0 | 7 | 1 | – | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 2 | 1 | 0 | 1 | 7 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 1 | 3 |
Mitochondria | 14 | 0 | 8 | 93 | 30 | 162 | – | 274 | 265 | 202 | 206 | 230 | 2 | 0 | 1 | 0 | 4 | 7 | 4 | 1 | 8 | 2 | 6 | 10 |
Nucleus | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 2 | 1 | 2 | 1 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1 | 5 | 2 | – | 29 | 4 | 12 | 3 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 3 | 6 |
Vac/Vac Membrane | 2 | 0 | 0 | 1 | 1 | 3 | – | 4 | 4 | 2 | 2 | 2 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 6 | 2 | 6 | 8 |
Unique Cell Count | 346 | 53 | 189 | 405 | 499 | 411 | 727 | 510 | 482 | 337 | 391 | 250 | 354 | 470 | 63 | 63 | 81 | 348 | 81 | 196 | 129 | 345 | 351 | |
Labelled Cell Count | 352 | 55 | 201 | 486 | 530 | 509 | 890 | 655 | 582 | 426 | 511 | 250 | 355 | 472 | 66 | 71 | 93 | 348 | 81 | 196 | 129 | 345 | 351 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.0 | 5.0 | 3.3 | 3.4 | 3.8 | 2.8 | – | 2.9 | 2.4 | 2.7 | 2.4 | 2.4 | 4.5 | 4.4 | 4.3 | 6.0 | 5.8 | 6.3 | 4.3 | 4.9 | 4.9 |
Std Deviation (1e-4) | 0.5 | 1.4 | 0.6 | 0.9 | 1.6 | 1.0 | – | 1.1 | 0.8 | 1.0 | 0.6 | 0.9 | 1.0 | 1.1 | 1.3 | 2.2 | 1.5 | 2.2 | 0.5 | 1.3 | 1.9 |
Intensity Change (Log2) | – | – | – | 0.05 | 0.22 | -0.21 | – | -0.17 | -0.45 | -0.27 | -0.42 | -0.44 | 0.46 | 0.45 | 0.4 | 0.88 | 0.84 | 0.96 | 0.4 | 0.58 | 0.59 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.8 | -1.5 | -5.7 | – | -6.8 | -7.9 | -7.3 | -9.6 | -9.3 | 0.8 | 1.7 | 2.1 | -0.8 | -2.5 | -3.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.0628 | 0.2326 | 0.049 | 0.2944 | -0.0884 | 0.1226 | -0.8089 | -0.059 | -0.28 | -0.2932 | -0.1279 | -0.2194 | 0.1896 | 1.5315 | 1.1594 | 0.9327 | 0.6442 | 1.1057 |
Actin | 0.0247 | 0.0011 | 0.0062 | 0.0052 | 0.0089 | 0.0034 | 0.0033 | 0.0014 | 0.0181 | 0.0037 | 0.0128 | 0.0019 | 0.0187 | 0.0001 | 0.0037 | 0.0002 | 0.0001 | 0.0002 |
Bud | 0.0004 | 0.016 | 0.0096 | 0.0002 | 0.0072 | 0.0017 | 0.0012 | 0.0023 | 0.0018 | 0.0013 | 0.0048 | 0.0016 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 |
Bud Neck | 0.0105 | 0.0004 | 0.0029 | 0.0018 | 0.0231 | 0.0018 | 0.0028 | 0.0003 | 0.0008 | 0.0003 | 0.0009 | 0.0028 | 0.0005 | 0 | 0.0001 | 0.0002 | 0.0002 | 0.0003 |
Bud Periphery | 0.0005 | 0.0029 | 0.0048 | 0.0002 | 0.0043 | 0.0032 | 0.0021 | 0.0049 | 0.0033 | 0.0019 | 0.0101 | 0.0022 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0002 |
Bud Site | 0.0032 | 0.0279 | 0.0102 | 0.001 | 0.0029 | 0.0002 | 0.008 | 0.006 | 0.0041 | 0.0009 | 0.0051 | 0.0006 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 |
Cell Periphery | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 |
Cytoplasm | 0.0893 | 0.1792 | 0.1539 | 0.0917 | 0.1665 | 0.2238 | 0.1858 | 0.3689 | 0.2603 | 0.2496 | 0.2063 | 0.3508 | 0.1809 | 0.3437 | 0.2885 | 0.35 | 0.4452 | 0.4694 |
Cytoplasmic Foci | 0.0175 | 0.008 | 0.0082 | 0.0107 | 0.0174 | 0.017 | 0.0128 | 0.0142 | 0.0128 | 0.0201 | 0.0314 | 0.0161 | 0.0055 | 0.0019 | 0.0032 | 0.0061 | 0.0039 | 0.004 |
Eisosomes | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0022 | 0.0008 | 0.0008 | 0.0008 | 0.0028 | 0.0007 | 0.0022 | 0.0018 | 0.0017 | 0.0032 | 0.0026 | 0.0014 | 0.0021 | 0.0007 | 0.0014 | 0.0019 | 0.0005 | 0.0034 |
Endosome | 0.0101 | 0.0006 | 0.001 | 0.0088 | 0.0089 | 0.0038 | 0.0111 | 0.0024 | 0.0076 | 0.0119 | 0.0422 | 0.0055 | 0.0019 | 0.0003 | 0.0034 | 0.0023 | 0.0006 | 0.0014 |
Golgi | 0.0037 | 0.0002 | 0.0003 | 0.0028 | 0.0016 | 0.0008 | 0.0018 | 0.0003 | 0.0027 | 0.0009 | 0.0159 | 0.0011 | 0.0013 | 0 | 0.0008 | 0.0001 | 0 | 0.0001 |
Lipid Particles | 0.0105 | 0.0002 | 0.0004 | 0.0063 | 0.0021 | 0.0016 | 0.0064 | 0.0006 | 0.0018 | 0.0017 | 0.0259 | 0.0017 | 0.002 | 0 | 0.001 | 0.0001 | 0.0001 | 0.0001 |
Mitochondria | 0.0034 | 0.0004 | 0.0016 | 0.0011 | 0.0007 | 0.0017 | 0.0031 | 0.0015 | 0.0097 | 0.0012 | 0.0299 | 0.0033 | 0.0002 | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0.0002 |
None | 0.7843 | 0.7491 | 0.7844 | 0.8304 | 0.7277 | 0.7194 | 0.7371 | 0.5846 | 0.6593 | 0.6674 | 0.5685 | 0.591 | 0.7806 | 0.65 | 0.6915 | 0.634 | 0.5462 | 0.5145 |
Nuclear Periphery | 0.0049 | 0.0006 | 0.001 | 0.0025 | 0.0025 | 0.0012 | 0.0023 | 0.0011 | 0.0014 | 0.0074 | 0.0037 | 0.0019 | 0.0007 | 0.0003 | 0.0024 | 0.0008 | 0.0002 | 0.0008 |
Nucleolus | 0.001 | 0.0006 | 0.0007 | 0.0011 | 0.0025 | 0.0003 | 0.0014 | 0.0005 | 0.0007 | 0.0007 | 0.0014 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 |
Nucleus | 0.003 | 0.0048 | 0.005 | 0.0029 | 0.0071 | 0.0018 | 0.0032 | 0.0036 | 0.0041 | 0.0073 | 0.0044 | 0.0044 | 0.0006 | 0.0017 | 0.0014 | 0.0015 | 0.0016 | 0.0033 |
Peroxisomes | 0.0214 | 0.0005 | 0.0028 | 0.0025 | 0.0012 | 0.0016 | 0.0029 | 0.0008 | 0.0035 | 0.0038 | 0.0172 | 0.0019 | 0.0028 | 0 | 0.0002 | 0.0001 | 0 | 0.0001 |
Punctate Nuclear | 0.0073 | 0.0058 | 0.0054 | 0.0292 | 0.0098 | 0.0152 | 0.0088 | 0.0032 | 0.0041 | 0.0142 | 0.0062 | 0.0096 | 0.0008 | 0.0004 | 0.0009 | 0.0016 | 0.0007 | 0.001 |
Vacuole | 0.001 | 0.0004 | 0.0005 | 0.0004 | 0.0019 | 0.0004 | 0.0026 | 0.001 | 0.0015 | 0.0019 | 0.0073 | 0.0011 | 0.0005 | 0.0003 | 0.0005 | 0.0006 | 0.0002 | 0.0009 |
Vacuole Periphery | 0.0006 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0002 | 0.0008 | 0.0002 | 0.0003 | 0.0006 | 0.0031 | 0.0003 | 0.0001 | 0 | 0.0002 | 0.0001 | 0 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 85.8203 | 98.6376 | 89.4586 | 85.9137 | 95.3741 | 55.1397 | 88.651 | 72.922 | 113.2181 | 75.2835 |
Translational Efficiency | 3.7613 | 2.841 | 2.9176 | 4.5804 | 3.9806 | 6.4932 | 3.0626 | 3.8398 | 2.5119 | 3.5355 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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