Standard name | |
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Human Ortholog | |
Description | E3 ubiquitin ligase; forms heterodimer with Rad6p to regulate K63 polyubiquitination in response to oxidative stress and to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control; interacts with RNA-binding protein Npl3p, linking histone ubiquitination to mRNA processing; Bre1p-dependent histone ubiquitination promotes pre-mRNA splicing |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | – | 0 | – | 0 | 0 | – | – | – | – | – | 0 | 0 | 0 | – | – | – | 1 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | – | 0 | – | 0 | 0 | – | – | – | – | – | 0 | 0 | 0 | – | – | – | 0 | 3 | 2 | 2 | 0 | 2 |
Bud Neck | 0 | 0 | – | 0 | – | 0 | 0 | – | – | – | – | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 1 | 0 | 0 |
Bud Site | 0 | 1 | – | 0 | – | 2 | 2 | – | – | – | – | – | 1 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | – | 0 | – | 0 | 0 | – | – | – | – | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 4 | – | 1 | – | 1 | 3 | – | – | – | – | – | 1 | 3 | 6 | – | – | – | 0 | 0 | 0 | 1 | 0 | 1 |
Endoplasmic Reticulum | 0 | 0 | – | 1 | – | 0 | 0 | – | – | – | – | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 0 | 2 | – | 0 | – | 0 | 0 | – | – | – | – | – | 1 | 0 | 0 | – | – | – | 1 | 1 | 2 | 1 | 1 | 1 |
Golgi | 0 | 0 | – | 0 | – | 0 | 0 | – | – | – | – | – | 0 | 0 | 0 | – | – | – | 0 | 1 | 0 | 1 | 0 | 0 |
Mitochondria | 3 | 12 | – | 6 | – | 5 | 7 | – | – | – | – | – | 16 | 7 | 3 | – | – | – | 1 | 0 | 1 | 0 | 1 | 0 |
Nucleus | 15 | 67 | – | 39 | – | 19 | 31 | – | – | – | – | – | 109 | 94 | 71 | – | – | – | 37 | 70 | 25 | 96 | 86 | 117 |
Nuclear Periphery | 0 | 0 | – | 0 | – | 0 | 0 | – | – | – | – | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 5 | 23 | – | 11 | – | 4 | 6 | – | – | – | – | – | 25 | 11 | 17 | – | – | – | 2 | 2 | 1 | 5 | 2 | 7 |
Peroxisomes | 0 | 2 | – | 0 | – | 0 | 1 | – | – | – | – | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 2 | – | 0 | – | 0 | 1 | – | – | – | – | – | 0 | 0 | 0 | – | – | – | 0 | 3 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 3 | – | 0 | – | 0 | 3 | – | – | – | – | – | 1 | 3 | 0 | – | – | – | 2 | 7 | 1 | 3 | 2 | 5 |
Unique Cell Count | 18 | 86 | 44 | 20 | 40 | 124 | 105 | 78 | 49 | 91 | 39 | 116 | 96 | 144 | ||||||||||
Labelled Cell Count | 24 | 116 | 58 | 31 | 54 | 154 | 118 | 97 | 49 | 91 | 39 | 116 | 96 | 144 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 6.1 | 6.1 | – | 6.2 | – | 6.1 | 6.6 | – | – | – | – | – | 7.2 | 7.4 | 7.7 | – | – | – | 7.0 | 6.6 | 7.0 |
Std Deviation (1e-4) | 0.9 | 1.6 | – | 1.2 | – | 1.3 | 1.4 | – | – | – | – | – | 1.6 | 1.5 | 2.2 | – | – | – | 1.9 | 2.5 | 2.3 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.069 | 0.0481 | 0.0486 | 0.001 | 0.17 | 0.0436 | 0.0118 | 0.0013 | 0.0249 | 0.0139 | 0.0503 | 0.0278 | – | – | – | – | – | – |
Bud | 0.0017 | 0.0009 | 0.0013 | 0.0015 | 0.0007 | 0.0005 | 0.0015 | 0.0009 | 0.0006 | 0.0001 | 0.0014 | 0.0031 | – | – | – | – | – | – |
Bud Neck | 0.0121 | 0.0041 | 0.0024 | 0.0039 | 0.0012 | 0.0016 | 0.0012 | 0.0002 | 0.0001 | 0.0001 | 0.0007 | 0.0009 | – | – | – | – | – | – |
Bud Periphery | 0.004 | 0.0013 | 0.0024 | 0.0046 | 0.0006 | 0.0025 | 0.0031 | 0.0013 | 0.0007 | 0.0003 | 0.0033 | 0.0043 | – | – | – | – | – | – |
Bud Site | 0.0319 | 0.0215 | 0.0235 | 0.0006 | 0.0037 | 0.0011 | 0.0028 | 0.002 | 0.001 | 0.0003 | 0.0023 | 0.0027 | – | – | – | – | – | – |
Cell Periphery | 0.0033 | 0.0004 | 0.0015 | 0.0005 | 0.0002 | 0.0009 | 0.0004 | 0.0002 | 0 | 0 | 0.0003 | 0.0001 | – | – | – | – | – | – |
Cytoplasm | 0.0212 | 0.019 | 0.0115 | 0.001 | 0.0004 | 0.0003 | 0.0157 | 0.0002 | 0.0002 | 0.0002 | 0.0012 | 0.001 | – | – | – | – | – | – |
Cytoplasmic Foci | 0.0143 | 0.0026 | 0.0024 | 0.0001 | 0.0158 | 0.0032 | 0.0026 | 0.0003 | 0.0005 | 0.034 | 0.0128 | 0.0017 | – | – | – | – | – | – |
Eisosomes | 0.0039 | 0.0003 | 0.0005 | 0 | 0.0004 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | – | – | – | – | – | – |
Endoplasmic Reticulum | 0.0147 | 0.0014 | 0.0012 | 0.0004 | 0.0001 | 0.001 | 0.0028 | 0.0001 | 0.0001 | 0.0002 | 0.004 | 0.0007 | – | – | – | – | – | – |
Endosome | 0.1068 | 0.0034 | 0.0182 | 0.0005 | 0.0054 | 0.0066 | 0.0242 | 0.0012 | 0.0006 | 0.009 | 0.061 | 0.0044 | – | – | – | – | – | – |
Golgi | 0.0434 | 0.0063 | 0.0086 | 0 | 0.0271 | 0.0065 | 0.0242 | 0.0006 | 0.0009 | 0.0211 | 0.0794 | 0.0021 | – | – | – | – | – | – |
Lipid Particles | 0.076 | 0.0022 | 0.0025 | 0.0001 | 0.0056 | 0.0167 | 0.0029 | 0.0041 | 0.0003 | 0.0273 | 0.0696 | 0.0014 | – | – | – | – | – | – |
Mitochondria | 0.0381 | 0.0098 | 0.0231 | 0.0009 | 0.0093 | 0.0251 | 0.1153 | 0.023 | 0.0094 | 0.0581 | 0.0781 | 0.005 | – | – | – | – | – | – |
None | 0.0086 | 0.0004 | 0.0007 | 0.0002 | 0.0001 | 0.0007 | 0.0035 | 0.0002 | 0.0002 | 0.0001 | 0.0015 | 0.0006 | – | – | – | – | – | – |
Nuclear Periphery | 0.1278 | 0.002 | 0.0119 | 0.0231 | 0.0002 | 0.0202 | 0.0914 | 0.0017 | 0.0063 | 0.0008 | 0.0166 | 0.0099 | – | – | – | – | – | – |
Nucleolus | 0.0228 | 0.0282 | 0.0099 | 0.0497 | 0.087 | 0.1011 | 0.1461 | 0.0181 | 0.0097 | 0.0045 | 0.1283 | 0.0826 | – | – | – | – | – | – |
Nucleus | 0.3533 | 0.8437 | 0.813 | 0.9011 | 0.662 | 0.7251 | 0.4242 | 0.9389 | 0.9414 | 0.7645 | 0.4563 | 0.8404 | – | – | – | – | – | – |
Peroxisomes | 0.0076 | 0.0021 | 0.0025 | 0.0001 | 0.0096 | 0.0119 | 0.0007 | 0.0005 | 0.0013 | 0.0633 | 0.0128 | 0.0019 | – | – | – | – | – | – |
Punctate Nuclear | 0.0133 | 0.0007 | 0.0065 | 0.0023 | 0.0002 | 0.0104 | 0.0713 | 0.0005 | 0.001 | 0.0009 | 0.0116 | 0.0074 | – | – | – | – | – | – |
Vacuole | 0.0147 | 0.0009 | 0.0055 | 0.0052 | 0.0002 | 0.0114 | 0.0133 | 0.0022 | 0.0001 | 0.0002 | 0.0029 | 0.0009 | – | – | – | – | – | – |
Vacuole Periphery | 0.0114 | 0.0008 | 0.0024 | 0.0032 | 0.0002 | 0.0092 | 0.0407 | 0.0024 | 0.0005 | 0.0009 | 0.005 | 0.0007 | – | – | – | – | – | – |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 36.7277 | 32.8323 | 32.0199 | 33.9393 | 40.1361 | 26.7439 | 34.9751 | 35.3687 | 31.8285 | 43.6273 |
Translational Efficiency | 0.9797 | 1.1833 | 0.9054 | 0.8855 | 0.8522 | 1.0976 | 0.7939 | 0.7324 | 0.7458 | 0.7671 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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