Standard name | |
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Human Ortholog | |
Description | Guanine nucleotide exchange factor for the GTPase Gtr1p; subunit of the HOPS endocytic tethering complex; vacuole membrane protein; functions as a Rab GTPase effector, interacting with both GTP- and GDP-bound conformations of Ypt7p; facilitates tethering and promotes membrane fusion events at the late endosome and vacuole; required for both membrane and protein trafficking; component of vacuole-mitochondrion contacts (vCLAMPs) important for lipid transfer between organelles |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 0 | 0 |
Bud | 1 | 2 | 5 | 7 | 5 | 12 | 26 | 28 | 24 | 34 | 39 | 29 | 0 | 0 | 0 | 7 | 1 | 4 | 2 | 0 | 0 | 2 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 28 | 31 | 31 | 41 | 76 | 72 | 145 | 163 | 135 | 185 | 151 | 203 | 30 | 30 | 72 | 112 | 66 | 36 | 3 | 5 | 0 | 3 | 3 | 4 |
Endoplasmic Reticulum | 2 | 6 | 0 | 1 | 1 | 3 | 3 | 1 | 3 | 1 | 0 | 2 | 9 | 8 | 1 | 25 | 15 | 10 | 0 | 1 | 1 | 0 | 3 | 1 |
Endosome | 182 | 30 | 240 | 165 | 326 | 107 | 157 | 89 | 45 | 31 | 16 | 48 | 29 | 29 | 97 | 153 | 157 | 125 | 240 | 139 | 217 | 111 | 177 | 160 |
Golgi | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 3 | 0 | 2 | 2 |
Mitochondria | 68 | 103 | 62 | 118 | 78 | 163 | 261 | 392 | 317 | 480 | 337 | 408 | 0 | 0 | 1 | 48 | 38 | 26 | 18 | 14 | 11 | 7 | 17 | 11 |
Nucleus | 12 | 15 | 2 | 1 | 3 | 1 | 4 | 10 | 2 | 5 | 0 | 2 | 22 | 22 | 13 | 14 | 12 | 7 | 5 | 6 | 1 | 4 | 3 | 2 |
Nuclear Periphery | 11 | 5 | 1 | 0 | 2 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 8 | 2 | 18 | 4 | 23 | 5 | 10 | 9 |
Nucleolus | 29 | 11 | 27 | 7 | 25 | 19 | 19 | 27 | 10 | 14 | 4 | 10 | 2 | 2 | 9 | 12 | 29 | 16 | 2 | 2 | 0 | 1 | 0 | 2 |
Peroxisomes | 0 | 0 | 15 | 6 | 19 | 5 | 27 | 18 | 11 | 16 | 2 | 12 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 33 | 4 | 61 | 33 | 62 | 32 | 53 | 65 | 39 | 39 | 26 | 43 | 16 | 16 | 41 | 61 | 63 | 23 | 4 | 6 | 10 | 5 | 5 | 3 |
Vac/Vac Membrane | 279 | 168 | 121 | 83 | 122 | 67 | 79 | 61 | 46 | 43 | 30 | 64 | 88 | 91 | 184 | 171 | 151 | 109 | 219 | 147 | 77 | 57 | 82 | 73 |
Unique Cell Count | 451 | 288 | 355 | 333 | 504 | 352 | 563 | 633 | 476 | 642 | 455 | 603 | 169 | 171 | 321 | 423 | 363 | 241 | 520 | 338 | 356 | 205 | 312 | 279 |
Labelled Cell Count | 646 | 375 | 565 | 462 | 721 | 481 | 775 | 856 | 636 | 850 | 606 | 823 | 200 | 202 | 419 | 606 | 544 | 359 | 520 | 338 | 356 | 205 | 312 | 279 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 5.0 | 5.4 | 5.1 | 5.2 | 4.4 | 4.1 | 4.0 | 3.9 | 3.4 | 3.6 | 3.7 | 6.1 | 6.1 | 6.4 | 4.9 | 5.6 | 6.9 | 6.1 | 6.6 | 6.9 |
Std Deviation (1e-4) | 0.6 | 0.8 | 1.6 | 1.6 | 1.9 | 1.5 | 1.4 | 1.1 | 1.3 | 1.1 | 0.9 | 0.9 | 0.7 | 0.7 | 0.8 | 1.1 | 1.3 | 1.7 | 1.3 | 1.6 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.05 | -0.3 | -0.39 | -0.44 | -0.46 | -0.65 | -0.59 | -0.54 | 0.18 | 0.18 | 0.25 | -0.12 | 0.06 | 0.36 | 0.18 | 0.29 | 0.36 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.3 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.5 | 2.8 | 4.4 | 6.4 | 6.5 | 7.0 | 7.4 | 8.3 | 8.7 | 3.0 | 3.0 | 4.9 | 6.4 | 3.7 | 2.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.7 | 3.9 | 0 |
Endosome | -4.8 | -0.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -10.8 | -10.9 | -9.7 | -8.7 | -6.6 | -3.9 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 5.4 | -0.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -5.8 | -5.8 | -7.7 | -2.4 | -2.7 | -2.3 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6.4 | 6.3 | 3.1 | 2.7 | 2.7 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -3.3 | -1.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.0 | -3.0 | -2.8 | -3.0 | 0.2 | -0.4 |
Peroxisomes | -1.8 | -0.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.3 | -4.3 | -4.0 | -3.2 |
SpindlePole | -2.8 | -2.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.3 | -2.4 | -1.6 | -1.1 | 0.1 | -2.6 |
Vacuole | -2.6 | -3.2 | -4.5 | -7.2 | -9.5 | -8.7 | -11.2 | -10.0 | -8.9 | 3.9 | 4.2 | 6.1 | 1.8 | 2.1 | 2.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.8124 | 1.1826 | 1.0155 | 0.8493 | 1.3962 | 0.9665 | 2.0718 | 2.4266 | 2.5457 | 1.8021 | 2.4221 | 2.4091 | 2.9153 | 2.8546 | 2.096 | 1.0957 | 1.8063 | 2.2782 |
Actin | 0.0412 | 0.0075 | 0.0018 | 0.0008 | 0.0165 | 0.0006 | 0.0009 | 0 | 0.0207 | 0.0008 | 0.0039 | 0.002 | 0.0016 | 0 | 0.0002 | 0 | 0 | 0 |
Bud | 0.0013 | 0.0026 | 0.0026 | 0.0004 | 0.001 | 0.0005 | 0.0005 | 0.0003 | 0.0005 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0001 |
Bud Neck | 0.0063 | 0.0049 | 0.003 | 0.0005 | 0.0007 | 0.0023 | 0.0008 | 0.0003 | 0.0008 | 0.0004 | 0.0007 | 0.0007 | 0.0017 | 0.0009 | 0.0004 | 0.0001 | 0.0001 | 0.0005 |
Bud Periphery | 0.0027 | 0.0032 | 0.0045 | 0.0004 | 0.0022 | 0.001 | 0.0011 | 0.0004 | 0.0007 | 0.0001 | 0.0006 | 0.0001 | 0.0004 | 0.0002 | 0.0004 | 0.0001 | 0 | 0.0002 |
Bud Site | 0.0091 | 0.0069 | 0.0032 | 0.0007 | 0.0019 | 0.0011 | 0.0031 | 0.0007 | 0.0051 | 0.0007 | 0.0016 | 0.0003 | 0.0081 | 0.0011 | 0.0013 | 0.0002 | 0.0001 | 0.0004 |
Cell Periphery | 0.0009 | 0.0007 | 0.0007 | 0.0001 | 0.0003 | 0.0002 | 0.0004 | 0.0002 | 0.0004 | 0.0001 | 0.0004 | 0.0002 | 0.0015 | 0.0018 | 0.0006 | 0 | 0 | 0.0003 |
Cytoplasm | 0.0113 | 0.0565 | 0.0275 | 0.0177 | 0.0082 | 0.0109 | 0.0037 | 0.0055 | 0.0087 | 0.0022 | 0.0105 | 0.0095 | 0.0397 | 0.0024 | 0.0436 | 0.0024 | 0.0015 | 0.0369 |
Cytoplasmic Foci | 0.0783 | 0.0832 | 0.0914 | 0.1214 | 0.0694 | 0.0749 | 0.0846 | 0.0811 | 0.0948 | 0.1136 | 0.0934 | 0.0885 | 0.0676 | 0.0456 | 0.0657 | 0.0684 | 0.1378 | 0.0614 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0.0007 | 0 | 0.0007 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0076 | 0.0051 | 0.0067 | 0.0004 | 0.0029 | 0.0014 | 0.001 | 0.0015 | 0.0052 | 0.0003 | 0.0012 | 0.0011 | 0.0095 | 0.0006 | 0.0101 | 0.0004 | 0.0004 | 0.002 |
Endosome | 0.481 | 0.4693 | 0.5556 | 0.616 | 0.5616 | 0.5709 | 0.5752 | 0.6151 | 0.585 | 0.6442 | 0.5726 | 0.6399 | 0.1964 | 0.545 | 0.4848 | 0.6226 | 0.6808 | 0.5031 |
Golgi | 0.035 | 0.0156 | 0.0273 | 0.0236 | 0.0549 | 0.0281 | 0.0449 | 0.0237 | 0.0511 | 0.0452 | 0.0382 | 0.0375 | 0.0112 | 0.0119 | 0.0348 | 0.0266 | 0.0236 | 0.0277 |
Lipid Particles | 0.0551 | 0.0743 | 0.0433 | 0.0804 | 0.0955 | 0.0757 | 0.0579 | 0.0352 | 0.0708 | 0.0637 | 0.0528 | 0.0806 | 0.1987 | 0.1333 | 0.0376 | 0.0455 | 0.0893 | 0.1815 |
Mitochondria | 0.0259 | 0.005 | 0.0376 | 0.0791 | 0.0713 | 0.0051 | 0.0175 | 0.0155 | 0.0266 | 0.0071 | 0.0274 | 0.0065 | 0.003 | 0.0014 | 0.0096 | 0.014 | 0.0014 | 0.0017 |
None | 0.0496 | 0.036 | 0.0231 | 0.001 | 0.0015 | 0.0019 | 0.0018 | 0.0295 | 0.0017 | 0.0002 | 0.089 | 0.01 | 0.0861 | 0.0008 | 0.0544 | 0.0008 | 0.0002 | 0.0044 |
Nuclear Periphery | 0.0348 | 0.0138 | 0.0059 | 0.0004 | 0.0342 | 0.0084 | 0.0028 | 0.0016 | 0.0052 | 0.0005 | 0.0012 | 0.0017 | 0.0294 | 0.0006 | 0.0255 | 0.0008 | 0.0007 | 0.0512 |
Nucleolus | 0.0032 | 0.003 | 0.0033 | 0.0005 | 0.002 | 0.0035 | 0.0008 | 0.0005 | 0.0008 | 0.0012 | 0.0023 | 0.0009 | 0.0164 | 0.0072 | 0.0032 | 0.0004 | 0.0002 | 0.001 |
Nucleus | 0.0088 | 0.0048 | 0.0072 | 0.0004 | 0.0053 | 0.0116 | 0.0008 | 0.0007 | 0.0008 | 0.0007 | 0.0011 | 0.0007 | 0.0195 | 0.001 | 0.0044 | 0.0003 | 0.0001 | 0.0034 |
Peroxisomes | 0.0105 | 0.0074 | 0.0109 | 0.0072 | 0.0184 | 0.0161 | 0.0188 | 0.012 | 0.027 | 0.0225 | 0.0142 | 0.0144 | 0.0069 | 0.0076 | 0.0047 | 0.0059 | 0.0062 | 0.0114 |
Punctate Nuclear | 0.0054 | 0.0066 | 0.0014 | 0.0005 | 0.0056 | 0.0029 | 0.0008 | 0.0005 | 0.0008 | 0.0004 | 0.0041 | 0.0011 | 0.1635 | 0.0005 | 0.0017 | 0.0004 | 0.0004 | 0.0013 |
Vacuole | 0.1016 | 0.162 | 0.1165 | 0.0389 | 0.0331 | 0.147 | 0.1429 | 0.1343 | 0.0705 | 0.0837 | 0.0712 | 0.0797 | 0.1261 | 0.2164 | 0.1622 | 0.1688 | 0.046 | 0.0976 |
Vacuole Periphery | 0.0301 | 0.0316 | 0.0264 | 0.0096 | 0.0133 | 0.036 | 0.0394 | 0.0414 | 0.0222 | 0.0121 | 0.0123 | 0.0245 | 0.0122 | 0.0219 | 0.0545 | 0.0422 | 0.0111 | 0.0137 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 11.3843 | 10.9597 | 10.6886 | 17.8279 | 8.1749 | 11.203 | 9.5775 | 14.531 | 13.1623 | 15.1591 |
Translational Efficiency | 0.4618 | 0.4106 | 0.444 | 0.379 | 0.5234 | 0.4548 | 0.5532 | 0.2961 | 0.4128 | 0.3235 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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