Standard name | |
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Human Ortholog | |
Description | Peroxisomal malate dehydrogenase; catalyzes interconversion of malate and oxaloacetate; involved in the glyoxylate cycle |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | – | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | – | 2 | 0 | 0 | 1 | 1 | 1 | 5 | 4 | 4 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 4 |
Bud Neck | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud Site | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 0 | – | 3 | 7 | 4 | 5 | 11 | 2 | 3 | 5 | 12 | 4 | 8 | 6 | 22 | 18 | 30 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 217 | 17 | – | 221 | 317 | 269 | 384 | 357 | 135 | 231 | 167 | 192 | 290 | 320 | 387 | 398 | 220 | 311 | 209 | 49 | 43 | 180 | 308 | 429 |
Endoplasmic Reticulum | 1 | 0 | – | 1 | 3 | 4 | 9 | 22 | 3 | 10 | 2 | 5 | 1 | 3 | 1 | 14 | 11 | 15 | 1 | 0 | 0 | 0 | 0 | 3 |
Endosome | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 |
Golgi | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 0 | 0 | – | 1 | 1 | 9 | 25 | 8 | 22 | 24 | 40 | 17 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 1 | 0 |
Nucleus | 0 | 0 | – | 4 | 2 | 0 | 7 | 9 | 2 | 6 | 5 | 5 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 |
Nuclear Periphery | 0 | 0 | – | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 0 | 0 | – | 4 | 5 | 12 | 41 | 24 | 14 | 55 | 10 | 22 | 0 | 0 | 1 | 15 | 6 | 2 | 0 | 0 | 1 | 0 | 1 | 3 |
Unique Cell Count | 218 | 17 | 227 | 326 | 288 | 446 | 418 | 167 | 314 | 209 | 243 | 291 | 322 | 389 | 411 | 230 | 320 | 218 | 51 | 49 | 188 | 321 | 452 | |
Labelled Cell Count | 220 | 17 | 237 | 335 | 298 | 473 | 439 | 182 | 334 | 235 | 262 | 297 | 333 | 396 | 450 | 258 | 361 | 218 | 51 | 49 | 188 | 321 | 452 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.0 | 12.4 | – | 11.1 | 11.1 | 9.8 | 9.1 | 9.9 | 8.1 | 8.6 | 8.4 | 8.9 | 14.0 | 15.3 | 15.2 | 25.6 | 26.0 | 25.7 | 12.9 | 13.0 | 13.9 |
Std Deviation (1e-4) | 1.7 | 2.4 | – | 2.6 | 1.6 | 1.5 | 1.5 | 1.6 | 1.6 | 1.9 | 1.9 | 1.9 | 2.1 | 2.3 | 2.2 | 5.6 | 5.2 | 5.1 | 4.4 | 3.0 | 2.9 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 8.382 | 10.9042 | 9.7036 | 8.708 | 7.3384 | 9.6911 | 7.8765 | 11.2161 | 10.1759 | 9.279 | 8.9413 | 9.4162 | 6.6293 | 8.4637 | 7.0656 | 7.3309 | 5.8984 | 7.824 |
Actin | 0.0344 | 0.0007 | 0.0086 | 0.0018 | 0.0411 | 0.0033 | 0.0319 | 0.0002 | 0.0064 | 0.0094 | 0.0149 | 0.0001 | 0.0201 | 0.0006 | 0.0457 | 0.0057 | 0.0104 | 0.0001 |
Bud | 0.0009 | 0.0001 | 0.0052 | 0.0002 | 0.0008 | 0.0002 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0004 | 0.0001 | 0.0014 | 0.0002 | 0.0007 | 0 |
Bud Neck | 0.0008 | 0.0001 | 0.0006 | 0.0006 | 0.0014 | 0.0014 | 0.0014 | 0.0001 | 0.0003 | 0.0006 | 0.0032 | 0.0008 | 0.0005 | 0.0001 | 0.0008 | 0.0005 | 0.0005 | 0.0005 |
Bud Periphery | 0.0015 | 0 | 0.0024 | 0.0001 | 0.0006 | 0.0002 | 0.0008 | 0 | 0.0002 | 0 | 0.0004 | 0 | 0.0003 | 0 | 0.0037 | 0 | 0.0007 | 0 |
Bud Site | 0.0049 | 0.0003 | 0.0025 | 0.0012 | 0.002 | 0.0002 | 0.0014 | 0.0002 | 0.0002 | 0.0002 | 0.001 | 0 | 0.0008 | 0.0001 | 0.0058 | 0.0002 | 0.0021 | 0 |
Cell Periphery | 0.0009 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0006 | 0 | 0.0001 | 0 |
Cytoplasm | 0.7709 | 0.9638 | 0.9189 | 0.8562 | 0.724 | 0.9267 | 0.8516 | 0.9904 | 0.9858 | 0.9545 | 0.9024 | 0.9892 | 0.8934 | 0.9926 | 0.8519 | 0.9197 | 0.7897 | 0.9912 |
Cytoplasmic Foci | 0.0224 | 0.0044 | 0.0116 | 0.0598 | 0.0314 | 0.0154 | 0.0154 | 0.0027 | 0.0012 | 0.0046 | 0.0022 | 0.0026 | 0.009 | 0.0019 | 0.0152 | 0.0319 | 0.035 | 0.0029 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0008 | 0 | 0.0001 | 0 | 0.0006 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0086 | 0.0014 | 0.0012 | 0.001 | 0.0026 | 0.0016 | 0.0065 | 0.0007 | 0.0007 | 0.0006 | 0.007 | 0.0014 | 0.004 | 0.0014 | 0.0016 | 0.0005 | 0.0008 | 0.0006 |
Endosome | 0.0692 | 0.0031 | 0.0151 | 0.0462 | 0.0607 | 0.0139 | 0.0299 | 0.0018 | 0.001 | 0.002 | 0.0166 | 0.0015 | 0.0196 | 0.0012 | 0.01 | 0.0066 | 0.0202 | 0.0013 |
Golgi | 0.0252 | 0.0017 | 0.0116 | 0.0057 | 0.0366 | 0.0151 | 0.0085 | 0.0001 | 0.0003 | 0.0024 | 0.0071 | 0.0001 | 0.0042 | 0.0002 | 0.0134 | 0.003 | 0.0153 | 0.0001 |
Lipid Particles | 0.0069 | 0.0001 | 0.0027 | 0.002 | 0.004 | 0.0018 | 0.009 | 0 | 0.0001 | 0.0069 | 0.0071 | 0 | 0.0032 | 0 | 0.0096 | 0.0047 | 0.033 | 0 |
Mitochondria | 0.0117 | 0.0198 | 0.0123 | 0.0009 | 0.0253 | 0.009 | 0.0133 | 0 | 0.0004 | 0.0006 | 0.0092 | 0.0002 | 0.0027 | 0 | 0.0184 | 0.0004 | 0.0521 | 0.0001 |
None | 0.0046 | 0.0017 | 0.0013 | 0.0101 | 0.018 | 0.0009 | 0.0074 | 0.0014 | 0.0009 | 0.001 | 0.0028 | 0.0009 | 0.0045 | 0.0006 | 0.001 | 0.0027 | 0.0007 | 0.0011 |
Nuclear Periphery | 0.0098 | 0.0003 | 0.0009 | 0.0005 | 0.0093 | 0.0004 | 0.0048 | 0.0004 | 0.0004 | 0.0005 | 0.0132 | 0.0004 | 0.0062 | 0.0003 | 0.0013 | 0.0004 | 0.0005 | 0.0002 |
Nucleolus | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0006 | 0 | 0.0003 | 0 | 0 | 0 | 0.0005 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0003 | 0 |
Nucleus | 0.0067 | 0.001 | 0.0015 | 0.0019 | 0.0074 | 0.0023 | 0.0029 | 0.0013 | 0.0014 | 0.0021 | 0.0054 | 0.0022 | 0.0026 | 0.0007 | 0.0011 | 0.001 | 0.001 | 0.0015 |
Peroxisomes | 0.0108 | 0.001 | 0.0018 | 0.003 | 0.0222 | 0.0063 | 0.0058 | 0 | 0.0001 | 0.0135 | 0.0012 | 0 | 0.0126 | 0 | 0.0131 | 0.0193 | 0.0329 | 0 |
Punctate Nuclear | 0.0035 | 0.0001 | 0.0005 | 0.0006 | 0.0075 | 0.0003 | 0.004 | 0.0002 | 0.0001 | 0.0006 | 0.0012 | 0.0002 | 0.0138 | 0.0001 | 0.0035 | 0.0023 | 0.0013 | 0.0001 |
Vacuole | 0.0042 | 0.0003 | 0.0008 | 0.0061 | 0.0031 | 0.0006 | 0.0025 | 0.0003 | 0.0002 | 0.0003 | 0.0018 | 0.0003 | 0.0014 | 0.0002 | 0.0008 | 0.0006 | 0.0016 | 0.0002 |
Vacuole Periphery | 0.0016 | 0.0001 | 0.0006 | 0.002 | 0.0011 | 0.0003 | 0.0011 | 0 | 0.0001 | 0.0001 | 0.0016 | 0.0001 | 0.0004 | 0 | 0.0005 | 0.0002 | 0.0009 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 78.5855 | 74.5517 | 70.9238 | 64.2623 | 60.302 | 70.5275 | 70.5471 | 75.0092 | 78.1382 | 81.8546 |
Translational Efficiency | 1.2826 | 1.0931 | 1.0754 | 1.4724 | 1.6194 | 1.7262 | 1.3394 | 1.4413 | 1.4118 | 1.3509 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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