Standard name
Human Ortholog
Description Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; functions as a chaperone under ATP-depleted oxidative stress conditions; subunit of GET complex, involved in ATP dependent Golgi to ER trafficking and insertion of tail-anchored (TA) proteins into ER membrane under non-stress conditions; binds as dimer to transmembrane domain (TMD) cargo, shielding TMDs from aqueous solvent; protein abundance increases under DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.07 0.05 0.07 0.05 0 0.06 0.07 0.1 0.09 0.06 0 0.06 0.07 0.06 0.05 0 0 0 0 0 0
Cytoplasm 0.87 0.87 0.82 0.86 0.83 0.8 0.73 0.66 0.71 0.67 0.7 0.67 0.94 0.99 0.98 0.88 0.95 0.92 0.44 0.74 0.65 0.78 0.74 0.76
Endoplasmic Reticulum 0.14 0.17 0.22 0.13 0.16 0.14 0.1 0.15 0.12 0.15 0.12 0.18 0.07 0 0 0 0 0 0.51 0.23 0.27 0.17 0.15 0.14
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0.06 0.17 0.18 0.13 0.14 0.08 0.07 0 0 0 0.1 0 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 4 5 4 8 6 7 0 0 0 0 0 0 2 0 0 0 3 3
Bud 1 1 0 1 0 0 2 0 2 3 4 2 1 1 0 0 1 0 0 0 0 0 2 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 5 4 13 18 26 29 23 18 21 31 31 24 14 7 18 28 12 22 0 0 0 1 3 0
Cytoplasm 194 188 259 232 397 341 365 286 265 304 225 179 222 280 315 328 202 385 104 152 227 249 293 303
Endoplasmic Reticulum 32 36 68 35 75 61 51 66 44 68 38 49 16 8 10 5 2 7 120 47 95 54 58 54
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 0 4 5
Golgi 0 0 1 0 0 1 2 0 1 0 0 0 0 0 1 3 0 0 0 0 3 0 6 9
Mitochondria 1 1 0 0 0 2 9 4 15 12 23 8 0 0 0 0 0 2 0 0 1 2 3 1
Nucleus 2 0 1 1 0 1 4 6 1 10 4 3 5 3 1 3 0 1 0 0 1 0 4 1
Nuclear Periphery 0 0 0 0 0 2 0 2 0 0 0 0 0 0 0 0 0 0 3 0 3 0 2 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Vac/Vac Membrane 1 0 6 6 25 27 85 76 48 62 25 20 0 1 0 37 7 23 2 2 6 2 8 4
Unique Cell Count 223 215 316 270 478 427 503 431 375 451 321 267 236 284 322 374 213 417 239 207 348 318 396 398
Labelled Cell Count 236 230 348 294 523 464 545 463 401 498 356 292 258 300 345 405 224 442 239 207 348 318 396 398


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 18.1 23.7 18.5 18.0 17.6 14.9 13.8 14.5 13.2 13.2 11.8 12.5 23.2 22.1 22.9 29.8 27.5 27.7 21.3 21.6 22.7
Std Deviation (1e-4) 2.9 4.4 3.4 2.5 2.7 2.5 2.8 3.0 3.9 2.7 2.6 2.7 4.1 3.6 3.8 8.0 6.7 7.3 4.3 4.8 4.8
Intensity Change (Log2) – – – -0.03 -0.07 -0.3 -0.42 -0.35 -0.48 -0.48 -0.64 -0.56 0.33 0.26 0.31 0.69 0.58 0.59 0.21 0.23 0.3


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.4 0.8 1.6 0.3 0 0.9 1.6 2.8 2.4 1.0 -1.1 0.9 1.9 0.8 0.7
Cytoplasm 1.3 0.4 -0.7 -3.1 -4.7 -3.5 -4.5 -3.5 -4.2 4.2 6.7 6.7 2.1 4.3 4.3
Endoplasmic Reticulum -2.7 -2.1 -2.6 -4.5 -2.2 -3.5 -2.3 -3.3 -1.0 -4.8 -6.9 -7.1 -8.6 -6.9 -8.8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 3.6 0 4.8 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.3 2.4 2.9 6.6 6.8 5.3 5.7 3.5 3.3 0 0 0 4.3 1.0 2.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 17.1142 20.7924 19.4869 17.4423 16.1794 19.2114 20.5026 22.3587 20.4336 19.4852 20.2267 20.0433 18.5996 21.7244 19.8663 17.4778 18.0363 20.2306
Actin 0.0145 0.0009 0.0025 0.0047 0.0062 0.0012 0.0002 0.0009 0.0004 0.0003 0.0002 0.0002 0.0135 0.0012 0.0046 0.021 0.0041 0.0036
Bud 0.0007 0.0007 0.0005 0.0011 0.0006 0.0001 0 0 0.0002 0 0 0 0.0011 0.0003 0.0001 0.0012 0 0.0001
Bud Neck 0.0015 0.0005 0.001 0.0016 0.0043 0.0029 0.0002 0.0004 0.0004 0.0013 0.0018 0.0017 0.0008 0.0003 0.0007 0.0051 0.0005 0.0016
Bud Periphery 0.0011 0.0001 0.0006 0.0011 0.0011 0.0001 0 0 0.0001 0 0 0 0.0008 0.0001 0.0002 0.0009 0.0001 0.0001
Bud Site 0.0025 0.0006 0.0052 0.0015 0.0004 0.0001 0 0.0001 0.0001 0 0 0 0.0019 0.0014 0.0019 0.0054 0 0.0001
Cell Periphery 0.0005 0.0007 0.0004 0.0003 0.0004 0.0001 0.0002 0.0003 0.0002 0 0 0.0001 0.0003 0.0004 0.0002 0.0003 0.0001 0.0001
Cytoplasm 0.7559 0.9271 0.8664 0.8077 0.8119 0.8687 0.8321 0.8492 0.826 0.9068 0.9019 0.8563 0.7538 0.9005 0.7863 0.7629 0.7927 0.8382
Cytoplasmic Foci 0.0115 0.0018 0.0025 0.0026 0.0021 0.0041 0.0006 0.001 0.0009 0.0009 0.0005 0.0006 0.0099 0.0094 0.0049 0.0114 0.0009 0.0009
Eisosomes 0.0001 0 0 0 0.0001 0 0 0 0 0 0 0 0.0002 0 0.0001 0.0003 0.0001 0
Endoplasmic Reticulum 0.0987 0.0483 0.0815 0.1 0.0481 0.0602 0.1296 0.1265 0.1395 0.0711 0.0686 0.114 0.1094 0.0558 0.1372 0.0718 0.0927 0.12
Endosome 0.0266 0.0009 0.0058 0.0183 0.0216 0.0128 0.0013 0.0008 0.0007 0.0005 0.0003 0.0006 0.0192 0.001 0.0051 0.0197 0.0094 0.0031
Golgi 0.0065 0.0001 0.0037 0.0044 0.0039 0.0033 0 0.0001 0 0 0 0 0.0025 0.0002 0.0019 0.0055 0.0033 0.0004
Lipid Particles 0.003 0.0017 0.0005 0.001 0.0022 0.0025 0.0001 0.0005 0.0003 0.0001 0 0.0001 0.0036 0.0018 0.0027 0.0154 0.0058 0.0002
Mitochondria 0.004 0.0001 0.0034 0.0039 0.014 0.001 0 0 0 0 0 0 0.0035 0.0001 0.0017 0.0153 0.0034 0.0004
None 0.0031 0.0006 0.0012 0.0007 0.0013 0.0007 0.0006 0.0004 0.0005 0.0004 0.0004 0.0005 0.0033 0.0063 0.0134 0.0019 0.0009 0.0004
Nuclear Periphery 0.0266 0.0073 0.0106 0.0234 0.036 0.0163 0.0178 0.0069 0.0147 0.0054 0.0082 0.0086 0.0407 0.0112 0.0221 0.0234 0.0522 0.0137
Nucleolus 0.0004 0 0.0001 0.0001 0.0002 0.0001 0 0 0 0 0 0.0001 0.0006 0.0001 0.0005 0.0009 0.0002 0
Nucleus 0.0273 0.0054 0.0099 0.02 0.0217 0.0198 0.0151 0.0109 0.0144 0.0123 0.0172 0.0155 0.0208 0.0078 0.0121 0.0165 0.0217 0.0151
Peroxisomes 0.0009 0 0.0001 0.0002 0.0001 0.0001 0 0 0 0 0 0 0.0006 0 0.0003 0.009 0.0001 0
Punctate Nuclear 0.0047 0.0005 0.0008 0.0011 0.0008 0.0012 0.0002 0.0003 0.0005 0.0004 0.0004 0.0004 0.0046 0.0006 0.0025 0.0031 0.001 0.0005
Vacuole 0.0086 0.0026 0.0025 0.0049 0.0183 0.0037 0.0016 0.0014 0.001 0.0004 0.0003 0.0011 0.0071 0.0014 0.0013 0.006 0.0078 0.0013
Vacuole Periphery 0.0012 0.0001 0.0004 0.0014 0.0045 0.0009 0.0001 0 0.0001 0 0 0.0001 0.0018 0.0001 0.0004 0.0028 0.0028 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 279.7363 236.1746 187.6224 239.1714 222.3158 190.5296 240.9029 228.1677 229.4605 253.7634
Translational Efficiency 1.3844 1.3806 1.5058 1.2737 1.495 2.0161 1.3302 1.3198 1.3715 1.3852

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; functions as a chaperone under ATP-depleted oxidative stress conditions; subunit of GET complex, involved in ATP dependent Golgi to ER trafficking and insertion of tail-anchored (TA) proteins into ER membrane under non-stress conditions; binds as dimer to transmembrane domain (TMD) cargo, shielding TMDs from aqueous solvent; protein abundance increases under DNA replication stress
Localization
Cell Percentages ER (62%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Get3

Get3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Get3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available