Standard name
Human Ortholog
Description Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.2 0.19 0.11 0.09 0.07 0.08 0 0 0 0 0 0 0.08 0 0.12 0.1 0.15 0.16 0.09 0.08 0 0.1 0.14 0.08
Bud 0 0 0.08 0.05 0.07 0.06 0 0.07 0.07 0.05 0.05 0.07 0 0 0.05 0 0 0 0 0 0 0.06 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.13 0.24 0.2 0.16 0.19 0.15 0.15 0.14 0.15 0.11 0.09 0.11 0.33 0.46 0.49 0.27 0.23 0.28 0.08 0.13 0.07 0.05 0.06 0
Endoplasmic Reticulum 0.07 0.16 0.1 0 0.08 0.14 0 0 0 0.08 0.06 0.05 0.13 0.16 0.1 0.05 0 0 0.05 0.05 0 0.07 0.1 0.07
Endosome 0.09 0.06 0.05 0 0.1 0 0 0.11 0 0 0 0 0 0.1 0.07 0.1 0 0 0.18 0.19 0.24 0.15 0.13 0.09
Golgi 0.53 0.37 0.07 0 0.05 0 0 0 0 0 0 0 0.38 0.27 0.15 0.43 0.54 0.45 0.36 0.31 0.24 0.37 0.42 0.63
Mitochondria 0.07 0.16 0.51 0.78 0.62 0.73 0.74 0.74 0.85 0.88 0.88 0.85 0.17 0 0.19 0.05 0.05 0.05 0.05 0 0.2 0.06 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.15 0.11 0.09 0 0.06 0.05 0.07 0.13 0 0.05 0 0.05 0.06 0 0 0.2 0.22 0.16 0.09 0.12 0.09 0.1 0.06 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 38 45 17 16 15 17 12 6 1 11 4 6 8 6 21 25 30 30 20 24 7 7 22 14
Bud 2 3 12 9 15 14 15 27 12 14 9 21 2 4 9 2 3 2 7 11 3 4 4 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0
Bud Site 0 0 0 0 2 0 4 8 2 3 5 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 6 3 2 1 0 1 2 1 3 6 10 3 1 4 7 4 6 0 0 0 0 1 0
Cytoplasm 25 56 30 27 40 33 52 53 27 33 16 32 34 70 83 67 45 52 18 38 14 3 10 2
Endoplasmic Reticulum 14 38 15 6 16 30 13 9 6 23 11 13 14 24 17 13 7 2 11 13 7 4 16 11
Endosome 17 13 8 6 20 3 15 42 0 1 0 5 4 15 12 26 5 8 42 54 50 11 21 16
Golgi 101 85 11 4 10 9 6 0 0 2 2 0 40 41 25 106 106 82 84 92 50 27 69 107
Mitochondria 13 38 76 135 128 160 248 276 153 252 154 239 18 5 32 13 10 10 12 12 42 4 5 6
Nucleus 0 1 0 2 0 1 2 1 1 0 0 2 0 1 0 1 2 0 0 2 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 1 0 1 2 0 0 0 0 0 0 1 1 1 0 0 0
Nucleolus 2 1 1 2 1 2 3 6 1 2 2 4 0 0 0 1 0 3 0 0 0 0 0 0
Peroxisomes 0 0 1 0 1 0 0 0 0 0 1 0 0 1 0 0 0 0 1 0 2 0 1 1
SpindlePole 3 1 2 1 2 1 3 7 1 1 0 2 0 0 1 5 4 5 4 4 4 0 0 0
Vac/Vac Membrane 29 26 13 2 12 10 24 50 3 15 4 13 6 6 4 51 44 30 21 35 18 7 10 5
Unique Cell Count 189 232 148 172 207 219 336 373 179 287 176 280 104 151 170 249 196 183 232 297 210 74 167 171
Labelled Cell Count 245 313 189 212 263 280 398 487 209 360 215 354 129 174 208 317 260 230 232 297 210 74 167 171


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.1 10.8 6.6 6.4 6.7 6.0 5.7 5.7 5.2 5.0 4.9 4.7 10.5 10.6 9.5 13.2 14.3 14.2 9.8 12.2 13.2
Std Deviation (1e-4) 1.8 1.6 0.9 1.1 1.2 0.9 1.1 1.1 1.2 1.0 1.2 1.1 1.9 1.4 1.4 2.5 2.6 2.2 2.1 1.8 2.3
Intensity Change (Log2) – – – -0.04 0.02 -0.13 -0.21 -0.21 -0.35 -0.39 -0.44 -0.48 0.67 0.69 0.52 1.0 1.12 1.1 0.57 0.89 1.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.6 -1.4 -1.2 -3.4 -4.9 -4.3 -3.1 0 0 -1.0 -2.4 0.2 -0.5 1.0 1.3
Bud -1.0 -0.3 -0.6 -1.6 -0.3 -0.5 -1.3 0 0 -2.1 -2.1 -1.0 -3.8 -3.0 -3.2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.1 -0.2 -1.3 -1.3 -1.7 -1.2 -2.5 -2.9 -2.5 2.2 4.8 5.3 1.5 0.6 1.7
Endoplasmic Reticulum -2.4 -0.8 1.0 -2.7 -3.8 -2.5 -0.7 0 0 0.8 1.5 0 -1.8 -2.5 -3.7
Endosome -0.8 1.5 0 -0.4 2.0 0 0 0 0 0 1.5 0.6 1.7 -1.4 -0.4
Golgi -2.2 -1.0 -1.4 -3.1 0 0 0 0 0 6.0 4.5 2.0 7.4 9.0 7.5
Mitochondria 5.1 2.0 4.3 4.8 5.0 6.7 8.4 0 0 -5.5 -9.3 -6.1 -10.7 -9.8 -9.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -3.2 -1.1 -1.6 -0.6 1.5 -3.0 -1.4 -2.6 -1.7 -0.9 -1.7 -2.5 3.1 3.4 2.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.5077 9.1906 8.1769 7.8023 6.2511 8.063 8.2315 9.4735 9.3677 8.4408 8.2899 8.8975 9.9879 10.8382 9.9648 9.6683 10.1923 10.2449
Actin 0.0744 0.0099 0.0304 0.0014 0.0261 0.0146 0.0298 0.0121 0.0354 0.036 0.0363 0.0056 0.0247 0.0066 0.0205 0.024 0.031 0.0068
Bud 0.0039 0.0042 0.0069 0.0005 0.0023 0.001 0.0008 0.0019 0.0021 0.0006 0.0006 0.0004 0.0008 0.0007 0.0017 0.0014 0.0009 0.0007
Bud Neck 0.0044 0.0021 0.003 0.0029 0.0009 0.0093 0.001 0.0018 0.0025 0.0049 0.0029 0.0015 0.0017 0.0006 0.002 0.0008 0.0041 0.0022
Bud Periphery 0.0025 0.0033 0.0036 0.0004 0.0023 0.0008 0.0006 0.0007 0.0011 0.0004 0.0007 0.0002 0.0005 0.0002 0.0009 0.0014 0.001 0.0004
Bud Site 0.0115 0.0233 0.0132 0.0006 0.0053 0.0018 0.0039 0.0181 0.0096 0.0063 0.0034 0.0005 0.0155 0.0068 0.0051 0.0042 0.0035 0.001
Cell Periphery 0.0007 0.0008 0.0005 0.0002 0.0002 0.0003 0.0002 0.0007 0.0002 0.0005 0.0001 0.0001 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001
Cytoplasm 0.0443 0.1118 0.049 0.0161 0.0739 0.0423 0.0447 0.1377 0.0679 0.0357 0.035 0.0422 0.0591 0.1014 0.0441 0.0092 0.0174 0.0387
Cytoplasmic Foci 0.0992 0.1461 0.1221 0.0787 0.0729 0.1059 0.1495 0.2227 0.148 0.1625 0.169 0.1615 0.1531 0.2094 0.1612 0.1244 0.1848 0.1332
Eisosomes 0.0009 0 0.0001 0 0.0003 0.0001 0.0002 0.0001 0.0001 0.0027 0.0002 0 0.0006 0 0 0.0001 0.0001 0
Endoplasmic Reticulum 0.0089 0.0087 0.0057 0.0024 0.0019 0.0047 0.0059 0.0026 0.0058 0.0065 0.0031 0.0035 0.0084 0.002 0.0027 0.0007 0.003 0.0035
Endosome 0.4216 0.4118 0.3983 0.6045 0.4122 0.4048 0.4509 0.3853 0.3991 0.4355 0.4585 0.4116 0.3852 0.4111 0.4046 0.3885 0.2963 0.368
Golgi 0.2035 0.2125 0.2814 0.2118 0.2606 0.3509 0.2373 0.1849 0.2866 0.2841 0.2001 0.3411 0.3013 0.2392 0.3259 0.3869 0.3965 0.4126
Lipid Particles 0.0206 0.0081 0.0053 0.0055 0.0502 0.0047 0.0103 0.0022 0.004 0.0017 0.0127 0.002 0.0059 0.0011 0.0021 0.0218 0.0037 0.0014
Mitochondria 0.0396 0.0181 0.0334 0.0053 0.0475 0.0354 0.0166 0.0066 0.0103 0.0085 0.0533 0.0141 0.0101 0.0076 0.0077 0.0179 0.0059 0.0204
None 0.0039 0.0004 0.0095 0.0001 0.0011 0.0003 0.0121 0.0005 0.001 0.0031 0.0006 0.0002 0.0098 0.0002 0.001 0.0001 0.0017 0.0002
Nuclear Periphery 0.0172 0.0015 0.0037 0.0014 0.0029 0.0007 0.0055 0.0004 0.0017 0.0005 0.0014 0.0003 0.0028 0.0003 0.001 0.0001 0.0047 0.0004
Nucleolus 0.0005 0.0005 0.0003 0.0001 0.0007 0.0001 0.0004 0.0001 0.0002 0.0002 0.0004 0 0.0008 0 0.0001 0.0001 0.0059 0
Nucleus 0.0053 0.0012 0.0006 0.0004 0.0009 0.0005 0.0014 0.0004 0.0008 0.0003 0.0004 0.0001 0.0023 0.0002 0.0003 0.0001 0.0107 0.0002
Peroxisomes 0.014 0.0043 0.0114 0.0013 0.0152 0.0032 0.0172 0.0075 0.0135 0.0043 0.0141 0.0089 0.0045 0.0065 0.0085 0.0142 0.0064 0.0044
Punctate Nuclear 0.005 0.0005 0.0003 0.0003 0.004 0.0003 0.0029 0.0005 0.0013 0.0007 0.0021 0.0003 0.0034 0.0002 0.0013 0.0003 0.0194 0.0003
Vacuole 0.0113 0.024 0.0128 0.0414 0.0121 0.0132 0.0055 0.0106 0.0057 0.0028 0.0024 0.003 0.0063 0.0034 0.0057 0.0018 0.0017 0.0028
Vacuole Periphery 0.0069 0.0072 0.0084 0.0245 0.0063 0.0052 0.0034 0.0028 0.003 0.0021 0.0029 0.0028 0.0028 0.0025 0.0035 0.0021 0.0013 0.0027

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 51.251 17.6673 18.2206 22.4505 31.7045 31.8049 17.2579 17.6762 17.067 33.8013
Translational Efficiency 1.2652 1.3145 1.0749 1.0102 1.1444 1.7124 1.1072 0.8224 1.0174 1.0658

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✔
Standard name
Human Ortholog
Description Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER)
Localization
Cell Percentages cytoplasm (24%), Golgi (4%), mixed (44%)
Cell Cycle Regulation Yes
cytoplasm - T/G1 (0.00065)
Golgi to ER - MA (5.5e-05)
Subcompartmental Group N/A

Pol3

Pol3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pol3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available