Standard name
Human Ortholog
Description Peripherally bound inner membrane protein of the mitochondrial matrix; involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0 0
Cytoplasm 0.94 0.96 0.99 0.99 0.97 0.94 0.9 0.9 0.89 0.92 0.81 0.81 0.97 1.0 0.99 0.55 0.37 0.48 0.7 0.67 0.72 0.87 0.83 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.24 0.16 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0 0 0 0 0.07 0.15 0.11 0.2 0.13 0.32 0.26 0 0 0 0.09 0.16 0.13 0.15 0.19 0.16 0.07 0.06 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.19 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.1 0.11 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.16 0.14 0.17 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 3 1 3 6 1 0 1 1
Bud 0 0 0 0 0 2 5 3 2 5 3 3 0 0 0 0 6 1 2 2 1 0 3 3
Bud Neck 1 1 9 2 3 9 8 15 7 8 1 4 0 0 0 0 2 0 1 1 0 0 0 1
Bud Site 0 0 0 0 0 0 1 0 0 1 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 3 2 3 0 1 2 0 0 2 0 1 0 3 2 8 8 3 1 0 2 0 0 2
Cytoplasm 246 262 223 214 227 298 352 291 266 300 188 175 34 187 169 80 42 49 174 203 143 91 139 218
Endoplasmic Reticulum 1 3 2 0 0 1 1 0 1 0 0 0 0 0 1 11 1 4 1 1 1 0 0 0
Endosome 0 0 0 0 2 2 3 0 0 2 0 0 0 0 0 11 28 16 6 7 1 0 0 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 0 2 0 0 0 0 0
Mitochondria 18 3 0 2 2 21 57 34 60 44 74 56 0 0 0 13 18 13 36 56 31 7 9 15
Nucleus 0 0 0 0 2 2 1 2 2 0 1 1 1 1 0 6 3 3 0 0 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 1 0 1 1
Nucleolus 1 0 0 0 1 0 0 1 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 1 0 0 0 0 0 2 0 0 0 0 0 0 0 4 11 20 0 0 0 0 0 1
SpindlePole 1 3 1 2 0 4 7 7 1 6 7 7 0 0 0 12 12 11 6 4 3 0 1 3
Vac/Vac Membrane 0 2 0 0 2 2 2 1 2 2 0 1 0 0 0 24 16 17 5 13 1 0 2 5
Unique Cell Count 261 274 226 217 233 316 391 322 299 326 231 215 35 187 171 146 115 103 250 302 199 105 168 262
Labelled Cell Count 268 280 237 223 239 342 439 357 341 370 276 248 35 191 173 172 153 138 250 302 199 105 168 262


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.1 4.1 4.4 4.5 4.7 3.6 3.3 3.9 3.1 3.1 2.8 3.1 4.8 4.6 4.6 6.6 6.1 5.6 4.0 4.6 4.8
Std Deviation (1e-4) 0.8 0.8 0.9 2.1 2.4 1.6 1.6 1.7 1.4 1.2 1.2 1.2 1.9 0.8 1.1 1.7 1.8 1.6 1.1 1.6 1.4
Intensity Change (Log2) – – – 0.05 0.11 -0.29 -0.41 -0.16 -0.49 -0.47 -0.64 -0.51 0.13 0.08 0.08 0.6 0.48 0.35 -0.13 0.08 0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 6.3 5.7 6.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.0246 -0.1568 0.0384 0.1943 0.2701 -0.0371 -0.9994 -0.5306 -0.6413 -0.4404 -0.4386 -0.4427 -0.5238 -1.1345 -1.2699 -1.0485 -1.9946 -0.8794
Actin 0.0334 0.0033 0.0108 0.0059 0.0487 0.0036 0.0142 0.0005 0.0019 0.0033 0.0044 0.003 0.0457 0.0018 0.0151 0.0254 0 0.0214
Bud 0.0034 0.0023 0.0024 0.0048 0.0029 0.001 0.0003 0.0003 0.0007 0.002 0.0099 0.0001 0.0016 0.0066 0.0009 0.0026 0 0.0023
Bud Neck 0.008 0.0005 0.0065 0.0032 0.001 0.0069 0.0027 0.0004 0.0009 0.0001 0.0014 0.0055 0.0115 0.0005 0.0002 0.0134 0 0.0007
Bud Periphery 0.0132 0.004 0.0067 0.0108 0.0064 0.0041 0.0006 0.0007 0.0013 0.0043 0.007 0.0003 0.0028 0.0069 0.0007 0.001 0 0.0033
Bud Site 0.0063 0.0177 0.011 0.0109 0.0185 0.0011 0.0012 0.0015 0.012 0.0002 0.016 0.0002 0.0158 0.0206 0.0014 0.016 0 0.0005
Cell Periphery 0.0004 0.0005 0.0004 0.0005 0.0002 0.0001 0.0002 0.0002 0.0002 0.0003 0.0004 0.0001 0.0014 0.0006 0.0001 0.0004 0 0.0001
Cytoplasm 0.0571 0.1272 0.0629 0.0871 0.0564 0.101 0.0342 0.0446 0.0323 0.0263 0.0197 0.0503 0.0816 0.0662 0.0975 0.0831 0.0527 0.1131
Cytoplasmic Foci 0.0478 0.0233 0.0383 0.0902 0.0345 0.0221 0.009 0.0092 0.0088 0.0044 0.0041 0.0129 0.0245 0.0065 0.013 0.0158 0.0034 0.0154
Eisosomes 0.0004 0.0002 0.0003 0.0003 0.001 0.0001 0.0004 0.0002 0.0002 0.0003 0.0001 0.0001 0.0007 0.0003 0.0002 0.0003 0 0.0003
Endoplasmic Reticulum 0.0029 0.0012 0.0012 0.0015 0.0055 0.0007 0.0009 0.0005 0.0004 0.0017 0.0006 0.0008 0.005 0.0012 0.003 0.0015 0.0006 0.0012
Endosome 0.0379 0.0102 0.0176 0.0278 0.0263 0.0105 0.0032 0.0006 0.0016 0.0023 0.0079 0.0028 0.0312 0.0011 0.008 0.0087 0.0007 0.0058
Golgi 0.0159 0.002 0.0066 0.0171 0.0166 0.0043 0.0014 0.0002 0.0003 0.0007 0.0021 0.0004 0.0127 0.0003 0.0037 0.0068 0.0001 0.0109
Lipid Particles 0.0141 0.0037 0.0099 0.0227 0.0137 0.0035 0.0023 0.0005 0.0008 0.0003 0.0061 0.0015 0.0264 0.0005 0.0059 0.0187 0.0001 0.005
Mitochondria 0.0079 0.0083 0.0149 0.0224 0.0081 0.007 0.0021 0.001 0.0012 0.022 0.0005 0.0008 0.0199 0.0022 0.0105 0.0114 0.0001 0.0116
None 0.7176 0.7864 0.7897 0.6245 0.7365 0.8209 0.9143 0.934 0.9211 0.8844 0.9061 0.9112 0.67 0.8228 0.8209 0.7039 0.9415 0.7533
Nuclear Periphery 0.0064 0.0005 0.0009 0.0015 0.0044 0.0009 0.0033 0.0003 0.0005 0.0012 0.003 0.0006 0.0073 0.0005 0.0019 0.001 0.0001 0.0015
Nucleolus 0.0007 0.0008 0.0012 0.0024 0.0007 0.0009 0.0012 0.0007 0.001 0.0007 0.0005 0.0004 0.0012 0.0061 0.0018 0.0017 0 0.0043
Nucleus 0.0034 0.0011 0.0012 0.0028 0.0016 0.0014 0.0011 0.001 0.002 0.0033 0.0018 0.0011 0.0192 0.0501 0.0031 0.0738 0.0001 0.0395
Peroxisomes 0.0139 0.0019 0.011 0.0509 0.0101 0.0066 0.0039 0.0008 0.0081 0.0005 0.0019 0.0031 0.0068 0.0018 0.0052 0.0109 0 0.0064
Punctate Nuclear 0.0044 0.0014 0.0025 0.0065 0.0021 0.0013 0.0027 0.0022 0.0036 0.0411 0.0056 0.0036 0.0059 0.0021 0.0039 0.0017 0 0.0024
Vacuole 0.0034 0.0031 0.0029 0.005 0.0035 0.0012 0.0006 0.0004 0.0009 0.0005 0.0005 0.0009 0.0056 0.0011 0.0016 0.0017 0.0004 0.0005
Vacuole Periphery 0.0017 0.0004 0.001 0.0012 0.0014 0.0004 0.0004 0.0001 0.0001 0.0002 0.0002 0.0002 0.0029 0.0001 0.0012 0.0005 0 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.8367 5.6044 0.6931 4.3182 2.7095 7.3102 6.611 9.5615 7.8192 6.5551
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –
Standard name
Human Ortholog
Description Peripherally bound inner membrane protein of the mitochondrial matrix; involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Mss2

Mss2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mss2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available