Standard name | |
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Human Ortholog | |
Description | Serine/threonine protein kinase, subunit of transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters; phosphorylates Ser5 residue of the PolII C-terminal domain (CTD) at gene promoters; relocalizes to the cytosol in response to hypoxia |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 0 | 3 | 3 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 3 | 4 | 6 | 12 | 7 | 14 | 13 | 10 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 3 | 2 | 14 | 13 | 21 | 23 | 16 | 5 | 5 | 15 | 6 | 12 | 35 | 61 | 13 | 3 | 4 | 0 | 0 | 1 | 1 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 2 | 5 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 |
Mitochondria | 43 | 2 | 8 | 2 | 4 | 47 | 120 | 95 | 135 | 121 | 153 | 123 | 2 | 0 | 0 | 1 | 3 | 4 | 1 | 0 | 0 | 1 | 8 | 7 |
Nucleus | 257 | 92 | 183 | 180 | 315 | 338 | 433 | 364 | 283 | 298 | 219 | 389 | 261 | 305 | 416 | 110 | 54 | 72 | 245 | 90 | 184 | 172 | 327 | 322 |
Nuclear Periphery | 0 | 0 | 0 | 3 | 0 | 2 | 7 | 2 | 6 | 1 | 5 | 2 | 0 | 3 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Nucleolus | 46 | 18 | 12 | 6 | 10 | 18 | 62 | 32 | 54 | 42 | 56 | 41 | 9 | 7 | 22 | 6 | 2 | 7 | 19 | 6 | 9 | 8 | 37 | 50 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1 | 3 | 0 | 5 | 3 | 1 | 2 | 1 | 6 | 2 | 5 | 5 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 3 |
Vac/Vac Membrane | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 6 | 0 | 3 | 2 | 1 | 1 | 1 | 7 | 8 | 6 | 3 | 3 | 3 | 5 | 10 | 31 | 44 |
Unique Cell Count | 276 | 99 | 191 | 196 | 333 | 379 | 521 | 429 | 349 | 351 | 292 | 457 | 279 | 345 | 489 | 135 | 62 | 83 | 273 | 104 | 207 | 204 | 419 | 442 |
Labelled Cell Count | 349 | 115 | 207 | 207 | 350 | 430 | 661 | 530 | 494 | 489 | 469 | 579 | 288 | 356 | 518 | 143 | 68 | 92 | 273 | 104 | 207 | 204 | 419 | 442 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.8 | 8.5 | 6.8 | 6.5 | 6.8 | 5.7 | 5.1 | 5.4 | 4.6 | 4.6 | 4.1 | 4.9 | 7.3 | 7.5 | 7.5 | 11.2 | 10.1 | 10.0 | 7.0 | 7.2 | 7.9 |
Std Deviation (1e-4) | 0.8 | 1.5 | 1.3 | 1.4 | 1.4 | 1.7 | 1.4 | 1.3 | 1.3 | 1.1 | 1.3 | 1.0 | 1.2 | 1.6 | 1.8 | 2.8 | 3.0 | 2.8 | 1.8 | 1.4 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.0 | -0.25 | -0.4 | -0.32 | -0.58 | -0.55 | -0.73 | -0.45 | 0.1 | 0.15 | 0.15 | 0.72 | 0.58 | 0.56 | 0.04 | 0.09 | 0.22 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3.0 | 1.9 | 2.6 | 2.2 | 1.8 | 0 | 0 | 2.4 | 0 | 2.0 | 4.0 | 4.6 | 3.6 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 3.1 | 5.8 | 5.5 | 0 | 0 | 0 | 6.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -1.6 | -0.6 | -2.7 | -4.4 | -3.9 | -4.8 | -3.8 | -6.0 | -3.9 | -1.1 | -2.9 | -3.9 | -4.2 | -2.4 | -2.7 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -1.5 | -1.8 | -0.8 | 2.2 | 0.5 | 0 | 0 | 0 | 1.1 | -1.6 | -2.6 | -1.0 | -0.7 | 0 | 0.6 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.8721 | 2.0785 | 1.7458 | 1.7031 | 1.337 | 1.7228 | 2.2002 | 2.3918 | 2.1661 | 1.7903 | 1.7657 | 2.1888 | 1.6236 | 1.819 | 1.2025 | 0.8518 | 0.8613 | 1.548 |
Actin | 0.0016 | 0 | 0.0008 | 0.0002 | 0 | 0 | 0.0426 | 0 | 0.0001 | 0.0008 | 0 | 0 | 0.0408 | 0 | 0.0085 | 0 | 0.1503 | 0.0001 |
Bud | 0.0006 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0009 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0006 | 0 | 0.0001 | 0 | 0.0014 | 0 |
Bud Neck | 0.0006 | 0 | 0.0004 | 0.0004 | 0.0001 | 0.0004 | 0.008 | 0 | 0.0002 | 0.0008 | 0.0003 | 0.0005 | 0.0012 | 0 | 0.0001 | 0 | 0.0014 | 0.0007 |
Bud Periphery | 0.0006 | 0 | 0.0004 | 0.0002 | 0 | 0 | 0.0014 | 0 | 0 | 0.0002 | 0.0002 | 0 | 0.0018 | 0 | 0.0001 | 0 | 0.0029 | 0 |
Bud Site | 0.0004 | 0 | 0.0003 | 0.0003 | 0 | 0 | 0.0206 | 0 | 0.0011 | 0.0006 | 0.0001 | 0 | 0.0011 | 0 | 0.0005 | 0 | 0.0023 | 0 |
Cell Periphery | 0.0003 | 0 | 0.0002 | 0.0002 | 0 | 0 | 0.0004 | 0 | 0 | 0.0001 | 0 | 0 | 0.0003 | 0 | 0 | 0 | 0.0003 | 0 |
Cytoplasm | 0.0045 | 0 | 0.0007 | 0.0029 | 0 | 0.0002 | 0.0049 | 0 | 0.008 | 0.0036 | 0.0001 | 0 | 0.0153 | 0 | 0.0026 | 0 | 0.005 | 0 |
Cytoplasmic Foci | 0.0016 | 0 | 0.0023 | 0.0045 | 0 | 0 | 0.023 | 0 | 0.0078 | 0.008 | 0 | 0 | 0.0072 | 0 | 0.0069 | 0 | 0.0165 | 0.0035 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.001 | 0 |
Endoplasmic Reticulum | 0.0016 | 0 | 0.0024 | 0.0022 | 0 | 0 | 0.0042 | 0 | 0.0003 | 0.0014 | 0 | 0 | 0.0043 | 0 | 0.0003 | 0 | 0.0074 | 0 |
Endosome | 0.0078 | 0 | 0.004 | 0.0071 | 0 | 0 | 0.0292 | 0 | 0.0073 | 0.0349 | 0 | 0 | 0.0069 | 0 | 0.0009 | 0 | 0.0523 | 0.0002 |
Golgi | 0.0013 | 0 | 0.0003 | 0.0004 | 0 | 0 | 0.0102 | 0 | 0.0009 | 0.0125 | 0 | 0 | 0.0032 | 0 | 0.0005 | 0 | 0.0165 | 0.0001 |
Lipid Particles | 0.0087 | 0 | 0.004 | 0.0037 | 0 | 0 | 0.0178 | 0 | 0.0077 | 0.0101 | 0 | 0 | 0.0152 | 0 | 0.001 | 0 | 0.0619 | 0.0009 |
Mitochondria | 0.0036 | 0 | 0.002 | 0.0005 | 0.0004 | 0 | 0.0083 | 0.0001 | 0.0011 | 0.005 | 0.0011 | 0.0001 | 0.0017 | 0.0001 | 0.0034 | 0.0001 | 0.0135 | 0.0001 |
None | 0.0014 | 0 | 0.0002 | 0.0018 | 0 | 0 | 0.0144 | 0 | 0.0005 | 0.0029 | 0.0001 | 0 | 0.0014 | 0 | 0.0067 | 0 | 0.0111 | 0.0001 |
Nuclear Periphery | 0.0147 | 0.0004 | 0.007 | 0.0128 | 0.0025 | 0.0004 | 0.0143 | 0.0004 | 0.0016 | 0.0138 | 0.0027 | 0.0002 | 0.0094 | 0.0003 | 0.0008 | 0.0002 | 0.0338 | 0.0002 |
Nucleolus | 0.0216 | 0.0211 | 0.0141 | 0.0145 | 0.0652 | 0.0537 | 0.028 | 0.0178 | 0.006 | 0.0162 | 0.1141 | 0.0645 | 0.0179 | 0.0174 | 0.0097 | 0.0136 | 0.0432 | 0.0532 |
Nucleus | 0.9152 | 0.9734 | 0.9502 | 0.9116 | 0.9284 | 0.9432 | 0.7492 | 0.9812 | 0.9545 | 0.8718 | 0.88 | 0.934 | 0.8514 | 0.982 | 0.9481 | 0.9859 | 0.5229 | 0.9295 |
Peroxisomes | 0.0025 | 0 | 0.0001 | 0.0003 | 0 | 0 | 0.007 | 0 | 0.0008 | 0.0017 | 0 | 0 | 0.0111 | 0 | 0.0073 | 0 | 0.0367 | 0.0016 |
Punctate Nuclear | 0.0068 | 0.0049 | 0.0074 | 0.0328 | 0.0029 | 0.0018 | 0.0087 | 0.0002 | 0.0006 | 0.0121 | 0.0008 | 0.0004 | 0.0067 | 0.0001 | 0.0019 | 0.0002 | 0.0116 | 0.0095 |
Vacuole | 0.003 | 0 | 0.0021 | 0.0024 | 0.0001 | 0.0001 | 0.0039 | 0.0001 | 0.0008 | 0.0025 | 0.0002 | 0.0001 | 0.0015 | 0 | 0.0002 | 0 | 0.0033 | 0.0001 |
Vacuole Periphery | 0.0016 | 0 | 0.001 | 0.0009 | 0.0001 | 0 | 0.0025 | 0 | 0.0003 | 0.001 | 0.0002 | 0 | 0.0007 | 0 | 0.0001 | 0 | 0.0046 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 23.362 | 14.9938 | 10.3313 | 14.7997 | 20.9699 | 13.1713 | 18.4088 | 16.4445 | 15.451 | 19.9363 |
Translational Efficiency | 0.788 | 1.0961 | 1.13 | 0.7187 | 0.7407 | 1.5788 | 1.0078 | 0.9797 | 1.0359 | 1.0299 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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