Standard name
Human Ortholog
Description Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0 0 0.06 0.05 0 0 0 0 0.07 0.09 0.14 0.05 0.11 0.18 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0 0 0 0.23 0 0.16 0.1 0.08 0.14 0 0 0 0.06 0 0 0 0 0 0 0 0
Nucleus 0.59 0.64 0.63 0.9 0.9 0.84 0.77 0.78 0.78 0.75 0.78 0.73 0.53 0.73 0.71 0.64 0.62 0.55 0.36 0.5 0.4 0.44 0.38 0.31
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.4 0.44 0.29 0.05 0 0.07 0.08 0.08 0.13 0.14 0.11 0.17 0.4 0.16 0.14 0.23 0.2 0.2 0.51 0.39 0.44 0.41 0.29 0.29
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05
Vac/Vac Membrane 0.08 0 0.06 0 0.07 0.08 0.05 0.09 0 0.06 0.06 0.06 0.14 0.05 0.07 0.11 0.13 0.05 0 0 0.05 0.06 0.17 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 4 2 1 5 7 3 0 0 0 0 0 0 5 2 4 1 3 5
Bud Neck 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 1 0 2 12 2 9 6 9 20 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 7 1 12 23 16 20 36 36 15 16 13 18 6 11 20 7 11 10 0 1 2 0 2 0
Endoplasmic Reticulum 0 0 1 0 1 0 0 0 0 0 0 0 1 0 0 4 4 3 0 0 2 0 0 2
Endosome 0 4 9 0 1 5 2 5 5 8 2 9 0 3 3 5 0 0 5 4 10 0 5 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 2 0 0 0
Mitochondria 2 10 2 3 1 11 146 16 80 60 49 78 0 0 1 9 1 1 2 0 1 0 2 2
Nucleus 137 124 245 405 437 454 485 537 378 453 457 420 43 86 98 95 63 30 97 117 183 28 58 44
Nuclear Periphery 0 0 1 2 2 7 5 2 4 7 4 5 0 1 0 1 1 0 0 0 2 0 1 2
Nucleolus 93 85 113 24 6 37 50 55 62 84 65 98 32 19 20 34 20 11 138 89 202 26 44 41
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 6 0 0 4 2 7 1 4 2 4 1 1 0 0 0 0 6 5 13 1 7 6
Vac/Vac Membrane 18 8 25 6 33 42 30 65 17 37 35 34 11 6 9 16 13 3 10 9 23 3 26 28
Unique Cell Count 232 195 386 449 486 538 633 689 487 601 588 573 81 118 138 149 102 55 271 232 458 65 155 142
Labelled Cell Count 257 232 415 464 497 582 772 727 572 680 643 689 94 127 152 171 113 58 271 232 458 65 155 142


Cytoplasm, Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.2 9.2 11.2 10.6 10.3 8.5 5.6 8.3 7.1 7.7 7.8 7.4 10.9 10.5 10.3 9.2 11.9 11.3 9.3 12.0 12.4
Std Deviation (1e-4) 2.7 1.7 2.1 2.2 2.1 1.8 1.5 1.8 1.7 1.8 1.9 2.1 2.3 2.3 2.5 2.5 3.2 3.4 1.9 2.3 2.4
Intensity Change (Log2) – – – -0.07 -0.11 -0.39 -0.98 -0.42 -0.65 -0.53 -0.51 -0.58 -0.03 -0.08 -0.11 -0.27 0.1 0.02 -0.26 0.11 0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 3.7 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.4 0.2 0.5 1.9 1.6 0 -0.4 -0.9 0 0 2.8 4.8 0.9 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 -1.7 0 -2.2 -1.5 -1.2 0 -0.9 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 1.9 9.9 2.2 8.0 6.0 5.4 7.2 0 0 0 0 0 0
Nucleus 9.3 9.4 7.3 4.5 5.1 4.6 4.0 4.8 3.2 -1.7 1.9 1.6 0.1 -0.3 -1.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -9.3 -12.0 -9.1 -9.0 -9.2 -6.1 -5.9 -7.2 -4.5 1.8 -2.8 -3.4 -1.5 -2.0 -1.4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -3.9 0.2 0.8 -1.2 1.7 -2.0 -0.2 -0.3 -0.3 2.2 -0.6 0 1.7 2.1 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.3273 6.044 5.2591 4.0917 4.7921 5.1261 9.496 10.274 9.5908 9.8399 8.8807 9.9547 7.151 8.1234 7.3824 7.1274 6.2835 7.3238
Actin 0.0153 0 0.0014 0.0607 0.0001 0 0.0105 0 0.0051 0 0 0 0.0105 0.0062 0.0004 0.0134 0.0416 0.0024
Bud 0.0002 0.0001 0.0002 0.0003 0.0001 0.0001 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001 0.0005 0.0001 0.0002 0.0002 0.0025 0.0001
Bud Neck 0.0009 0.0006 0.0013 0.0011 0.0009 0.0025 0.0029 0.0007 0.0008 0.0015 0.0013 0.0021 0.0009 0.0006 0.0005 0.0013 0.0045 0.0025
Bud Periphery 0.0006 0.0001 0.0004 0.0005 0.0002 0.0003 0.0003 0.0001 0.0005 0.0002 0.0003 0.0003 0.0012 0.0001 0.0003 0.0003 0.0051 0.0003
Bud Site 0.001 0.0003 0.001 0.0009 0.0002 0.0004 0.0018 0.0003 0.0012 0.0004 0.0005 0.0003 0.0022 0.0007 0.0006 0.0006 0.0104 0.0005
Cell Periphery 0.0003 0.0001 0.0002 0.0001 0.0001 0.0002 0.0004 0.0002 0.0004 0.0001 0.0002 0.0002 0.0003 0.0001 0.0001 0.0001 0.0006 0.0001
Cytoplasm 0.0013 0.0001 0.0001 0.0003 0 0.0001 0.0056 0.0003 0.0009 0.0001 0.0001 0.0001 0.011 0.0001 0.0002 0.0005 0.0032 0.0006
Cytoplasmic Foci 0.0015 0 0.0004 0.0009 0 0.0012 0.0147 0.0001 0.0105 0 0 0.0012 0.0088 0.0005 0.0028 0.0015 0.0043 0.0019
Eisosomes 0.0004 0 0.0002 0.0003 0 0 0.0008 0 0.0001 0 0 0 0.0005 0 0 0 0.0006 0
Endoplasmic Reticulum 0.0034 0 0.0001 0.0005 0 0 0.0025 0.0002 0.0013 0 0 0.0001 0.0014 0 0.0003 0.0001 0.0021 0.0004
Endosome 0.008 0.0001 0.0007 0.0021 0.0001 0.0003 0.0106 0.0004 0.004 0.0001 0.0002 0.0009 0.0074 0.0003 0.0034 0.0006 0.0077 0.0054
Golgi 0.0026 0 0.0005 0.0025 0 0.0001 0.0032 0 0.0012 0 0 0.0002 0.0026 0.0006 0.001 0.0018 0.0045 0.006
Lipid Particles 0.0068 0.0001 0.0004 0.0009 0.0002 0.0018 0.0126 0.0003 0.0035 0.0001 0.0001 0.0064 0.0168 0.0003 0.0051 0.0025 0.0189 0.0018
Mitochondria 0.0051 0.0002 0.0269 0.0017 0.0005 0.0007 0.0028 0.0001 0.003 0.0001 0.0003 0.0001 0.0042 0.0002 0.0029 0.0008 0.0417 0.0022
None 0.0016 0 0.0001 0.0005 0 0 0.0028 0.0002 0.0014 0 0.0001 0 0.0089 0 0.0002 0.0001 0.0106 0.0003
Nuclear Periphery 0.0193 0.0016 0.0009 0.0108 0.0376 0.0026 0.0094 0.0069 0.0025 0.0009 0.0308 0.0018 0.0066 0.0009 0.0049 0.0032 0.0102 0.0024
Nucleolus 0.4924 0.4866 0.6027 0.3694 0.476 0.5838 0.5174 0.5536 0.6098 0.6085 0.3694 0.5948 0.4141 0.4794 0.5395 0.4687 0.3596 0.5386
Nucleus 0.4314 0.5078 0.3593 0.5426 0.4772 0.4015 0.3835 0.4311 0.3447 0.3856 0.5916 0.3866 0.479 0.5055 0.431 0.4802 0.4382 0.4273
Peroxisomes 0.0013 0 0.0003 0.0008 0 0.0004 0.0018 0 0.0023 0 0 0.0001 0.0036 0.0005 0.0015 0.0208 0.0057 0.0015
Punctate Nuclear 0.0026 0.0007 0.0018 0.0019 0.0058 0.0027 0.0142 0.0035 0.0038 0.0009 0.0039 0.0028 0.0157 0.0032 0.0038 0.0027 0.023 0.0046
Vacuole 0.0019 0.0012 0.0009 0.0006 0.0004 0.0009 0.001 0.0014 0.0023 0.0011 0.0009 0.0014 0.0022 0.0004 0.0009 0.0006 0.0028 0.0008
Vacuole Periphery 0.0019 0.0004 0.0003 0.0007 0.0004 0.0003 0.0011 0.0004 0.0006 0.0002 0.0003 0.0005 0.0014 0.0001 0.0006 0.0002 0.0025 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 56.6912 43.2621 38.5228 44.845 37.6477 31.7848 37.4934 38.8018 35.8852 46.533
Translational Efficiency 0.8953 0.9618 1.1548 0.728 1.1034 1.4025 1.002 0.7939 1.0082 0.9369

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7)
Localization
Cell Percentages nucleus (49%), nucleolus (18%), mixed (33%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Rrp42

Rrp42


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rrp42-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available